LOCUS       Exported               13223 bp ds-DNA     circular SYN 01-MAY-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    PBNeo_TetO_Cdk12_isoform1NT
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 13223)
  AUTHORS   Sara Dubbury
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 1, 2019 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13223
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    600..615
                     /label=M13F
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     LTR             complement(746..946)
                     /label=pBac 3'- LTR
                     /label=pBac\3'-LTR
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=19"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    complement(1021..1246)
                     /label=BGH polyA
                     /label=BGH\polyA
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=21"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    1278..1283
                     /label=XbaI
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     primer_bind     complement(1297..1305)
                     /label=stop
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=28"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    complement(1306..1347)
                     /label=V5
                     /note="ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff00ff
                     vntifkey=21"
                     /note="color: #ff00ff; direction: LEFT"
     protein_bind    1349..1373
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    complement(1350..1356)
                     /label=attR2
                     /note="ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff00ff
                     vntifkey=21"
                     /note="color: #ff00ff; direction: LEFT"
     misc_feature    1387..1413
                     /note="SJD35"
                     /note="color: #50ff50; direction: RIGHT"
     CDS             complement(1393..5847)
                     /codon_start=1
                     /label=CDK12 Isoform 1 Coding Sequence
                     /note="color: #993366"
                     /translation="MPNSERHGGKKDGSGGASGTSQPSSGGGSSNSRERHRLVSKHKRH
                     KSKHSKDVGLVTPEAASLGTIIKPLVEYDDISSDSDTFSDDTAFKSDRRENEERRGTDR
                     SDRLHRHRHHQHRRSRDLLKTKQTEKEKNQEVSKSGSMKDRVSGSSKRSVEGSDDYGKA
                     QLSKSGSKESRSSKMHKEKTRKERELKSGYKDRSKSHRKRETPKSYKTVASPKRRSRSP
                     HRKWSDSSKQDDSPSGASYGQDYDLSPPRSHTSSNYDSYKKSPGSTSRRQSISPPYKEP
                     SAYQSSTRSPSPYSRRQRSVSPYSRRRSSSYERSGSYSGRSPSPYGRRRSSSPFLSKRS
                     LSRSPLPSRKSMKSRSRSPAYSRHSSSHSKKKRSGSRSRHSSISPVRLPLNSSLGAELS
                     RKKKERAAAAAAAKMDGKESKSSPIILPKKEKLEVKESGLESKKLPRSIKSEKSTPDTE
                     LVTVAHSNPEVKHCLDTGKVRLDENLQKHPAKDLKAQGTKDVKPVAPKEVIVTSKETET
                     SEKETLPPLPTITSPPPLPATTPPPQTPPLPPLPPLPAIPLQPPLPPPQPPFSQVPVSS
                     TSILPSSPHPRTSTLSSQTNSQPPVQVSMKTQVSITAAIPHLKTSTLPPLPLPPLLPGD
                     DDMDSPKETLPSKPAKKEKEQRTRHLLTDLPLPPELPGGDPSPPDSPEPKAITPPQQPY
                     KKRPKICCPRYGERRQTESDWGKRCVDKFDIIGIIGEGTYGQVYKAKDKDTGELVALKK
                     VRLDNEKEGFPITAIREIKILRQLVHQSVVNMKEIVTDKQDALDFKKDKGAFYLVFEYM
                     DHDLMGLLESGLVHFSEDHIKSFMKQLMEGLDYCHKKNFLHRDIKCSNILLNNSGQIKL
                     ADFGLARLYNSEESRPYTNKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKP
                     IFQANLELAQLELISRLCGSPCPAVWPDVIKLPYFNTMKPKKQYRRRLREEFSFIPSAA
                     LDLLDHMLTLDPSKRCTAEQTLQSDFLKDVELSKMAPPDLPHWQDCHELWSKKRRRQRQ
                     SGIVIEDPPPSKASRKETTSGTTAEPVKNNSPAPPQPAPVKAEPGPGDAVGLGDITQQL
                     NQSELAVLLNLLQSQTDLSIPQMAQLLNIHSNPEMQQQLEALNQSISALTEASSQQQDS
                     ESIAPEESLKEVPSVPVVLPPAEQTTPEASNTPADMQNVLAVLLSQLMKTQEPAGNLEE
                     NTNDKNSGPQGPRRTPTMPQEEAAACPPHILPPEKRPPEPPGPPPPPPPPPLVEGDLSS
                     APQELNPAVTAALLQLLSQPEAEPPGHLPHEHQALRPMEYSTRSHPNRTYGNTDGPETG
                     FSSADTDERSSGPALTESLVQTPVKNRTFSGSVSHLGESNSYQGTGSVQFPGDQDLRFT
                     RVPLALHSVVGQPFLKSEGNSNSVVHAETKLQNYGELGPGTTGANSSGTTLQWGGPAQS
                     YGKPYRGAARVLPRGGRGRGVPY"
     misc_feature    5825..5868
                     /note="SJD103"
                     /note="color: #50ff50"
     misc_feature    5845..5856
                     /label=Kozak
                     /note="color: #993300"
     misc_feature    complement(5845..5847)
                     /label=StartCodon
                     /note="color: #339966; direction: LEFT"
     protein_bind    complement(5883..5907)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     misc_feature    complement(5893..5907)
                     /label=attR1
                     /note="ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff00ff
                     vntifkey=21"
                     /note="color: #ff00ff; direction: LEFT"
     misc_feature    5918..5923
                     /label=SpeI
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     misc_feature    complement(5951..6388)
                     /label=TetO
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#00ff00
                     vntifkey=21"
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    complement(6389..6394)
                     /label=XhoI
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    complement(6395)
                     /label=LoxP_mutation
                     /note="lox-P site
                     ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff00ff
                     vntifkey=21"
                     /note="color: #ff00ff; direction: LEFT"
     misc_feature    complement(6397..6593)
                     /label=bpA
                     /note="bovine growth hormone polyA_site
                     ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff00ff
                     vntifkey=21"
                     /note="color: #ff00ff; direction: LEFT"
     CDS             complement(6632..7435)
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /label=neo
                     /note="ApEinfo_fwdcolor=#0000ff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#0000ff
                     vntifkey=4"
                     /note="color: #0000ff"
                     /db_xref="GI:13492216"
                     /protein_id="AAK28133.1"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(7444..7769)
                     /label=pgk(1)
                     /note="PGK
                     ApEinfo_fwdcolor=#80ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=pgk
                     ApEinfo_revcolor=#999999
                     vntifkey=29"
                     /note="color: #999999; direction: LEFT"
     intron          complement(7770..7939)
                     /label=sequenced
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=15"
                     /note="color: #ffc0cb; direction: LEFT"
     promoter        complement(7770..7939)
                     /label=pgk
                     /note="PGK
                     ApEinfo_fwdcolor=#80ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#999999
                     vntifkey=29"
                     /note="color: #999999; direction: LEFT"
     promoter        complement(7940..8220)
                     /label=SV40 promoter /origin(1) promoter
                     /label=SV40\promoter/origin(1)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=SV40\promoter/origin
                     ApEinfo_revcolor=pink
                     vntifkey=30"
                     /note="color: #ffc0cb; direction: LEFT"
     promoter        complement(8221..8259)
                     /label=SV40 promoter /origin promoter
                     /label=SV40\promoter/origin
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=30"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_binding    8260..8279
                     /label=HIV flap/nuclear import signal
                     /label=HIV\flap/nuclear\import\signal
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=20"
                     /note="color: #ffc0cb"
     misc_feature    join(8282,8287..8291)
                     /label=TetO(1)
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=TetO
                     ApEinfo_revcolor=#00ff00
                     vntifkey=21"
                     /note="This feature has 2 segments:
                        1: 8282 .. 8282 / #00ff00
                        2: 8287 .. 8291 / #00ff00"
     misc_feature    8301..8305
                     /label=ClaI
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     misc_feature    join(8314..8319,8330..8378)
                     /label=UpdatedBarcodeFragment
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="This feature has 2 segments:
                        1: 8314 .. 8319 / #00ffff
                        2: 8330 .. 8378 / #00ffff"
     misc_feature    join(8314..8319,8330..8338)
                     /label=HW146
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="This feature has 2 segments:
                        1: 8314 .. 8319 / #00ffff
                        2: 8330 .. 8338 / #00ffff"
     misc_feature    complement(8343..8368)
                     /label=HW147
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: LEFT"
     LTR             8382..8687
                     /label=pBac 5'- LTR
                     /label=pBac\5'-LTR
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=19"
                     /note="color: #ffc0cb"
     misc_feature    complement(9180..9196)
                     /label=M13R
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    9521..11420
                     /label=CEN-URA3
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ff00"
     misc_feature    11459..12140
                     /label=colEI ori
                     /note="ApEinfo_fwdcolor=#c0c0c0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #c0c0c0"
     misc_feature    12237..13094
                     /label=Amp
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg
      661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
      721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa
      781 agataatcat gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
      841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
      901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct
      961 tctataaagt aacaaaactt ttatcgaatt cctgcagccc agcttgatcc cgcctcagaa
     1021 gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
     1081 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     1141 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     1201 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
     1261 cgggtttaaa cgggccctct agaggttagc gctagctcat tactacgtag aatcgagacc
     1321 gaggagaggg ttagggatag gcttaccaac cactttgtac aagaaagctg ggtcgaattc
     1381 gcccttctcg agttaataag gaactcctct ccctcttccc cctcgtggta ggactcttgc
     1441 agcccccctg tagggttttc cataagactg agctgggccc ccccactgaa gcgttgttcc
     1501 tgagctgttg gccccagtgg ttcccggtcc cagctcccca tagttttgca atttggtctc
     1561 tgcatgtacc acagagttgc tatttccctc agacttgagg aatggttgcc caaccactga
     1621 gtgtaatgct aagggaaccc tggtaaaacg gaggtcctgg tcccctggga actgcactga
     1681 ccctgtgccc tggtagctgt tggactcccc aaggtggctc acagagcctg agaaggtcct
     1741 gttcttcacc ggggtctgaa ccaaagattc tgtcaaggct ggaccagaac tgcgttcatc
     1801 agtgtcagcg gaactgaacc ctgtctcagg cccatcagta tttccgtaag tcctgtttgg
     1861 atgggatcgg gtggagtatt ccattggtct caaggcctgg tgctcatgtg gcaggtggcc
     1921 aggaggctct gcttcaggct gggataaaag ttgcagcaag gcggctgtca cggcgggatt
     1981 caactcctgg ggggcgctgg aaagatcgcc ttcaaccaga gggggtggag gtggcggcgg
     2041 tggaggtcca gggggctcag ggggcctctt ctctggtgga agaatgtgag gaggacatgc
     2101 tgctgcctcc tcctgtggca ttgtaggagt tcttcggggc ccctgtggcc cactattctt
     2161 gtcattggtg ttttcctcca ggttacctgc tggctcttgg gttttcatca gctgactcaa
     2221 gagaactgcc aacacattct gcatgtcagc tggtgtgttt gaagcttcag gagttgtctg
     2281 ttcagcagga ggcaggacca caggtacaga aggtacctcc ttcaatgatt cttcaggggc
     2341 tatggattct gagtcctgct gctgggaact ggcttcagtc agtgcactaa tagactgatt
     2401 caaggcttca agctgctgtt gcatctctgg attggagtgg atattaagca gctgtgccat
     2461 ctgagggatg ctcaggtcag tttggctctg aagcaggttt aataacactg ccaattcact
     2521 ttgattcaac tgctgtgtga tgtcaccaag gcctactgca tcccctggac caggctctgc
     2581 cttgacagga gcaggctgag gtggtgctgg gctattgttt ttcacaggct cagctgttgt
     2641 ccctgaggta gtttcttttc tagaagcttt ggacggaggt ggatcttcta tcacaatacc
     2701 actctgtcgc tgccgtcgac gtttcttact ccacaattca tggcaatcct gccagtgagg
     2761 taggtctgga ggtgccattt tgctgagttc cacatcttta agaaagtcac tctgtagggt
     2821 ctgttcagct gtgcacctct tgctaggatc cagtgtcagc atgtggtcca atagatcaag
     2881 tgccgctgaa ggaatgaaag agaattcttc tcttaggcgt ctcctgtatt gcttcttcgg
     2941 tttcatggtg ttgaagtagg gcagcttgat aacatcaggc cacactgctg gacaaggact
     3001 accacagaga cgactgataa gttctagctg agccagttct aaattggctt gaaaaatagg
     3061 tttctttgtg aacagttctc caaggatgca cccacagctc caaacatcaa tggctggtgt
     3121 gtatctttcc tctccaagaa gaagctctgg aggtcgatac caaagagtga tgactttgtt
     3181 tgtgtaaggg cgactctctt cagagttata gagccgagca agtccaaaat ctgccagttt
     3241 gatttgcccg ctgttattca gcaaaatgtt agaacattta atatcccgat ggaggaaatt
     3301 ctttttgtga cagtaatcca gtccttccat tagctgtttc ataaatgact tgatatggtc
     3361 ctcagaaaag tgcaccaaac ctgattcaag cagtcccatt aagtcatggt ccatatattc
     3421 aaatacaagg taaaaggcac ctttgtcctt cttgaaatcc agtgcatctt gtttgtctgt
     3481 gacaatttcc ttcatgttta caacactctg gtgaactaat tgacgaagaa tcttgatctc
     3541 ccggatggct gtgattggga agccctcttt ctcattgtcc agccgaacct tcttcagagc
     3601 tactagttct cctgtgtctt tgtccttggc tttatatact tggccatagg tcccctctcc
     3661 aataatccca ataatgtcaa acttgtccac acagcgcttc ccccaatcac tttctgtttg
     3721 tcttctttct ccataacgtg gacaacaaat ttttggtctc tttttatatg gttgttgagg
     3781 tggtgtaatt gcctttggct ctggagaatc tggaggcgat ggatctcctc ctggtagctc
     3841 aggaggaaga ggcaagtctg taagcaagtg gcgagtcctc tgttccttct ctttctttgc
     3901 aggctttgaa ggaagtgttt cttttggact atccatgtca tcatctccag gtaatagggg
     3961 agggaggggc agaggaggca atgttgaagt cttcagatgt ggaatagcag ctgttataga
     4021 tacttgagtc ttcatagaaa cctgtacagg gggctgagaa tttgtctgag aggatagagt
     4081 agatgtcctt gggtgaggag aagagggtaa aattgaagta cttgaaacag gaacttgact
     4141 aaatggtggt tggggaggag gcagaggtgg ctgcagcgga atagctggta gtggaggcaa
     4201 aggtggcaaa gggggtgtct gaggtggagg ggtagtagct ggtaaagggg gtggagaagt
     4261 aattgtggga agaggtggaa gggtctcctt ttctgatgtc tctgtctcct ttgaagtaac
     4321 aatcacctct ttcggtgcta caggtttaac gtcctttgtt ccctgtgctt tcaaatcctt
     4381 agcaggatgc ttctgcaagt tctcatccaa cctaaccttc cctgtgtcta aacaatgttt
     4441 tacctctggg tttgaatgtg ctacagtaac cagttcagta tctggggtcg atttttctga
     4501 ttttatactt ctgggtaact ttttagactc taaccctgac tccttcacct caagtttctc
     4561 ttttttaggc aaaattatag gtgaactctt ggactctttt ccatccattt ttgctgctgc
     4621 agcagctgct gctctttcct tcttttttct actgagttca gctcccaggc tggaattcaa
     4681 tggaagcctg acaggtgaga tactggaatg acgactgcgt gacccggatc gcttcttttt
     4741 actatgagaa gatgagtgtc ttgaatatgc aggacttcta cttctggact tcatggattt
     4801 cctactgggg agtggactcc gactcagaga cctcttgctc aggaaagggc tgcttgatcg
     4861 ccttcggcca taggggctgg gtgatctccc gctgtaagag ccgctccttt catagctgga
     4921 ggaccgtctc cggctatagg gactcacaga cctctgtcgt cggctgtaag gactgggtga
     4981 ccgagtgctg gactggtaag cagaaggctc tttgtaaggt gggctgattg actgccttct
     5041 tgaggtactt ccaggactct tcttgtagga gtcatagttg ctagaagtgt gagaccttgg
     5101 ggggctaaga tcgtagtctt ggccataaga agctccggaa gggctgtcat cttgcttgga
     5161 actgtcagac catttcctat ggggactcct ggatctccgt ttagggctag ccacggtttt
     5221 gtaacttttg ggtgtttccc ttttccgatg acttttactc cggtccttgt atccagactt
     5281 taactctcgc tctttgcggg tcttctcctt gtgcattttg gacgacctgg attccttgct
     5341 gccgcttttg gatagctggg ccttcccata atcatcactc ccctccacgg accgtttgga
     5401 actgcccgat acccggtcct tcatagatcc agatttggag acttcctgat ttttttcctt
     5461 ttccgtctgt ttagttttta gcaagtctcg ggaccgccgg tgctggtggt gacgatgtcg
     5521 gtgcaggcga tcgctccgat ccgttccccg acgttcctcg ttctccctcc ggtctgattt
     5581 gaaggccgtg tcatcggaga aggtgtctga atcagagctg atgtcatcgt actccaccag
     5641 tggtttgatt atggtaccca aagatgccgc ttcgggggtc accagcccca cgtctttgga
     5701 gtgcttggac ttatgccgct tgtgcttcga caccaagcgg tgacgctccc tgctgttgga
     5761 gctgccacct cccgatgacg gctgcgaagt tccagaagct cctccgctcc cgtccttctt
     5821 gcccccatgt ctctcggaat tgggcatggt ggcggcgagc tcagtagtaa gggcgaattc
     5881 ggagcctgct tttttgtaca aacttgttga tatctgcact agtggatccg agctcggtac
     5941 gaattcgggg ccgcggaggc tggatcggtc ccggtgtctt ctatggaggt caaaacagcg
     6001 tggatggcgt ctccaggcga tctgacggtt cactaaacga gctctgctta tataggcctc
     6061 ccaccgtaca cgcctactcg acccgggtac cgagctcgac tttcactttt ctctatcact
     6121 gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact
     6181 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct
     6241 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
     6301 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
     6361 ttctctatca ctgataggga gtggtaaact cgagttgcct gctattgtct tcccaatcct
     6421 cccccttgct gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat
     6481 gcgatgcaat ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca
     6541 aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg
     6601 ctgatcagcg agctctagag aattgatccc ctcagaagaa ctcgtcaaga aggcgataga
     6661 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     6721 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     6781 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     6841 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg
     6901 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
     6961 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
     7021 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
     7081 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
     7141 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
     7201 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg
     7261 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
     7321 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
     7381 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatggccgat cccatattgg
     7441 ctgcaggtcg aaaggcccgg agatgaggaa gaggagaaca gcgcggcaga cgtgcgcttt
     7501 tgaagcgtgc agaatgccgg gcctccggag gaccttcggg cgcccgcccc gcccctgagc
     7561 ccgcccctga gcccgccccc ggacccaccc cttcccagcc tctgagccca gaaagcgaag
     7621 gagcaaagct gctattggcc gctgccccaa aggcctaccc gcttccattg ctcagcggtg
     7681 ctgtccatct gcacgagact agtgagacgt gctacttcca tttgtcacgt cctgcacgac
     7741 gcgagctgcg gggcgggggg gaacttcctg actaggggag gagtagaagg tggcgcgaag
     7801 gggccaccaa agaacggagc cggttggcgc ctaccggtgg atgtggaatg tgtgcgaggc
     7861 cagaggccac ttgtgtagcg ccaagtgccc agcggggctg ctaaagcgca tgctccagac
     7921 tgccttggga aaagtactgc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     7981 ctcagaggca gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     8041 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     8101 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     8161 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     8221 tggggagcct ggggactttc cacaccctaa ctgacacaca cagcagagat ccagtttggt
     8281 tagatctcga ggtcgacggt atcgttggcc ggtactcgag aagcgtatga gatcggaaga
     8341 gcggttcagc aggaatgccg agaccgcgcc aacgataagc tatctataac aagaaaatat
     8401 atatataata agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt
     8461 taaatcttaa aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat
     8521 agttcaaaat cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg
     8581 cgactgagat gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat
     8641 atttcaagaa tgcatgcgtc aattttacgc agactatctt tctagggtta atctagctgc
     8701 atcaggatca tatcgtcggg tcttttttca attgctcagt catcgcccaa gctggcgcta
     8761 tctgggcatc ggggaggaag aagcccgtgc cttttcccgc gaggttgaag cggcatggaa
     8821 agagtttgcc gaggatgact gctgctgcat tgacgttgag cgaaaacgca cgtttaccat
     8881 gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt gaactgttcg ttcaggccac
     8941 ctgggatacc agttcgtcgc ggcttttccg gacacagttc cggatggtca gcccgaagcg
     9001 catcagcaac ccgaacaata ccggcgacag ccggaactgc cgtgccggtg tgcagattaa
     9061 tgacagcggt gcggcgctgg gatattacgt cagcgaggac gggtatcctg gctggatgcc
     9121 gcagaaatgg acatggatac cccgtgagtt acccggcggg cgcgcttggc gtaatcatgg
     9181 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9241 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9301 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9361 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9421 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9481 atacggttat ccacagaatc aggggataac gcaggaaaga cctgggtcct tttcatcacg
     9541 tgctataaaa ataattataa tttaaatttt ttaatataaa tatataaatt aaaaatagaa
     9601 agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc cgaagatgta aaagactcta
     9661 gggggatcgc caacaaatac taccttttat cttgctcttc ctgctctcag gtattaatgc
     9721 cgaattgttt catcttgtct gtgtagaaga ccacacacga aaatcctgtg attttacatt
     9781 ttacttatcg ttaatcgaat gtatatctat ttaatctgct tttcttgtct aataaatata
     9841 tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt gtttattttt ttttcttcat
     9901 tccgtaactc ttctaccttc tttatttact ttctaaaatc caaatacaaa acataaaaat
     9961 aaataaacac agagtaaatt cccaaattat tccatcatta aaagatacga ggcgcgtgta
    10021 agttacaggc aagcgatccg tcctaagaaa ccattattat catgacatta acctataaaa
    10081 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
    10141 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    10201 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
    10261 catcagagca gattgtactg agagtgcacc acgcttttca attcaattca tcattttttt
    10321 tttattcttt tttttgattt cggtttcttt gaaatttttt tgattcggta atctccgaac
    10381 agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg
    10441 ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca
    10501 ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta
    10561 gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt
    10621 cattggatgt tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa
    10681 tttgtttact aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta
    10741 agccgctaaa ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg
    10801 ctgacattgg taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat
    10861 gggcagacat tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc
    10921 aggcggcaga agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat
    10981 gcaagggctc cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg
    11041 acaaagattt tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt
    11101 acgattggtt gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc
    11161 aacagtatag aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa
    11221 gaggactatt tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag
    11281 gctgggaagc atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa
    11341 atgcatgtat actaaactca caaattagag cttcaattta attatatcag ttattaccct
    11401 gcggtgtgaa ataccgcaca gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    11461 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    11521 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    11581 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    11641 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    11701 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    11761 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    11821 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    11881 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    11941 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    12001 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    12061 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    12121 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    12181 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    12241 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    12301 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    12361 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    12421 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    12481 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    12541 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    12601 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    12661 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    12721 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    12781 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    12841 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    12901 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    12961 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    13021 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    13081 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    13141 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    13201 cacatttccc cgaaaagtgc cac
//
