LOCUS       PB-GFP-Gal8             7422 bp ds-DNA     circular     01-MAY-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          complement(4881..4903)
                     /label="M13_pUC_rev_primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4811..4845
                     /label="5' ITR specific sequence"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    join(7377..7422,1..17)
                     /label="3' ITR specific sequence"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     regulatory      447..734
                     /label="CAG_enhancer"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(4917..4946)
                     /label="lac_promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          5149..5168
                     /label="SV40pro_F_primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      5245..5376
                     /label="SV40_PA_terminator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        complement(4864..4882)
                     /label="M13_reverse_primer"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      3955..4456
                     /label="rb_glob_PA_terminator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        5052..5231
                     /label="SV40_promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    join(7373..7422,1..380)
                     /label="3' Terminal Repeat"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2315..2344
                     /label="Y66 (EGFP)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4475..4850
                     /label="5' Terminal Repeat"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(2177..2198)
                     /label="EGFP_N_primer"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2785..2806
                     /label="EGFP_C_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     gene            2135..2848
                     /label="EGFP"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2849..2857
                     /label="Linker"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     misc_feature    2858..3937
                     /label="Gal8"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     feature         complement(314..347)
                     /label="loxP"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     origin          5087..5164
                     /label="SV40_origin"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          2041..2060
                     /label="pCAG_F_primer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    381..2082
                     /label="CAG Promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          5333..5352
                     /label="EBV_rev_primer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2126..2135
                     /label="Kozac consensus"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     origin          complement(5600..6219)
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     gene            complement(6374..7234)
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(7276..7304)
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 CGTAAAATTG ACGCATGTGT TTTATCGATC TGTATATCGA GGTTTATTTA TTAATTTGAA
       61 TAGATATTAA GTTTTATTAT ATTTACACTT ACATACTAAT AATAAATTCA ACAAACAATT
      121 TATTTATGTT TATTTATTTA TTAAAAAAAA ACAAAAACTC AAAATTTCTT CTATAAAGTA
      181 ACAAAACTTT TAAACATTCT CTCTTTTACA AAAATAAACT TATTTTGTAC TTTAAAAACA
      241 GTCATGTTGT ATTATAAAAT AAGTAATTAG CTTAACTTAT ACATAATAGA AACAAATTAT
      301 ACTTATTAAT CGCATAACTT CGTATAATGT ATGCTATACG AAGTTATGCG ATTAAGGGAT
      361 CTGTAGGGCG CAGTAGTCCA ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC
      421 ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC
      481 CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA
      541 TAGGGACTTT CCATTGACGT CAATGGGTGG ACTATTTACG GTAAACTGCC CACTTGGCAG
      601 TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC
      661 CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT
      721 ACGTATTAGT CATCGCTATT ACCATGGGTC GAGGTGAGCC CCACGTTCTG CTTCACTCTC
      781 CCCATCTCCC CCCCCTCCCC ACCCCCAATT TTGTATTTAT TTATTTTTTA ATTATTTTGT
      841 GCAGCGATGG GGGCGGGGGG GGGGGGGGCG CGCGCCAGGC GGGGCGGGGC GGGGCGAGGG
      901 GCGGGGCGGG GCGAGGCGGA GAGGTGCGGC GGCAGCCAAT CAGAGCGGCG CGCTCCGAAA
      961 GTTTCCTTTT ATGGCGAGGC GGCGGCGGCG GCGGCCCTAT AAAAAGCGAA GCGCGCGGCG
     1021 GGCGGGAGTC GCTGCGTTGC CTTCGCCCCG TGCCCCGCTC CGCGCCGCCT CGCGCCGCCC
     1081 GCCCCGGCTC TGACTGACCG CGTTACTCCC ACAGGTGAGC GGGCGGGACG GCCCTTCTCC
     1141 TCCGGGCTGT AATTAGCGCT TGGTTTAATG ACGGCTCGTT TCTTTTCTGT GGCTGCGTGA
     1201 AAGCCTTAAA GGGCTCCGGG AGGGCCCTTT GTGCGGGGGG GAGCGGCTCG GGGGGTGCGT
     1261 GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT GCGGCCCGCG CTGCCCGGCG GCTGTGAGCG
     1321 CTGCGGGCGC GGCGCGGGGC TTTGTGCGCT CCGCGTGTGC GCGAGGGGAG CGCGGCCGGG
     1381 GGCGGTGCCC CGCGGTGCGG GGGGGCTGCG AGGGGAACAA AGGCTGCGTG CGGGGTGTGT
     1441 GCGTGGGGGG GTGAGCAGGG GGTGTGGGCG CGGCGGTCGG GCTGTAACCC CCCCCTGCAC
     1501 CCCCCTCCCC GAGTTGCTGA GCACGGCCCG GCTTCGGGTG CGGGGCTCCG TGCGGGGCGT
     1561 GGCGCGGGGC TCGCCGTGCC GGGCGGGGGG TGGCGGCAGG TGGGGGTGCC GGGCGGGGCG
     1621 GGGCCGCCTC GGGCCGGGGA GGGCTCGGGG GAGGGGCGCG GCGGCCCCGG AGCGCCGGCG
     1681 GCTGTCGAGG CGCGGCGAGC CGCAGCCATT GCCTTTTATG GTAATCGTGC GAGAGGGCGC
     1741 AGGGACTTCC TTTGTCCCAA ATCTGGCGGA GCCGAAATCT GGGAGGCGCC GCCGCACCCC
     1801 CTCTAGCGGG CGCGGGCGAA GCGGTGCGGC GCCGGCAGGA AGGAAATGGG CGGGGAGGGC
     1861 CTTCGTGCGT CGCCGCGCCG CCGTCCCCTT CTCCATCTCC AGCCTCGGGG CTGCCGCAGG
     1921 GGGACGGCTG CCTTCGGGGG GGACGGGGCA GGGCGGGGTT CGGCTTCTGG CGTGTGACCG
     1981 GCGGCTCTAG AGCCTCTGCT AACCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG
     2041 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCTGC AGTCGACGGT
     2101 ACCGCGGGCC CGGGATCCAC CGGTCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC
     2161 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT
     2221 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC
     2281 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA
     2341 GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC
     2401 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG
     2461 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA
     2521 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA
     2581 CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA
     2641 CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG
     2701 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA
     2761 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT
     2821 CACTCTCGGC ATGGACGAGC TGTACAAGGA CATGTCCATG ATGTTGTCCT TAAACAACCT
     2881 ACAGAATATC ATCTATAACC CGGTAATCCC GTTTGTTGGC ACCATTCCTG ATCAGCTGGA
     2941 TCCTGGAACT TTGATTGTGA TACGTGGGCA TGTTCCTAGT GACGCAGACA GATTCCAGGT
     3001 GGATCTGCAG AATGGCAGCA GCATGAAACC TCGAGCCGAT GTGGCCTTTC ATTTCAATCC
     3061 TCGTTTCAAA AGGGCCGGCT GCATTGTTTG CAATACTTTG ATAAATGAAA AATGGGGACG
     3121 GGAAGAGATC ACCTATGACA CGCCTTTCAA AAGAGAAAAG TCTTTTGAGA TCGTGATTAT
     3181 GGTGCTGAAG GACAAATTCC AGGTGGCTGT AAATGGAAAA CATACTCTGC TCTATGGCCA
     3241 CAGGATCGGC CCAGAGAAAA TAGACACTCT GGGCATTTAT GGCAAAGTGA ATATTCACTC
     3301 AATTGGTTTT AGCTTCAGCT CGGACTTACA AAGTACCCAA GCATCTAGTC TGGAACTGAC
     3361 AGAGATAAGT AGAGAAAATG TTCCAAAGTC TGGCACGCCC CAGCTTCCTA GTAATAGAGG
     3421 AGGAGACATT TCTAAAATCG CACCCAGAAC TGTCTACACC AAGAGCAAAG ATTCGACTGT
     3481 CAATCACACT TTGACTTGCA CCAAAATACC ACCTATGAAC TATGTGTCAA AGAGGCTGCC
     3541 ATTCGCTGCA AGGTTGAACA CCCCCATGGG CCCTGGACGA ACTGTCGTCG TTAAAGGAGA
     3601 AGTGAATGCA AATGCCAAAA GCTTTAATGT TGACCTACTA GCAGGAAAAT CAAAGGATAT
     3661 TGCTCTACAC TTGAACCCAC GCCTGAATAT TAAAGCATTT GTAAGAAATT CTTTTCTTCA
     3721 GGAGTCCTGG GGAGAAGAAG AGAGAAATAT TACCTCTTTC CCATTTAGTC CTGGGATGTA
     3781 CTTTGAGATG ATAATTTATT GTGATGTTAG AGAATTCAAG GTTGCAGTAA ATGGCGTACA
     3841 CAGCCTGGAG TACAAACACA GATTTAAAGA GCTCAGCAGT ATTGACACGC TGGAAATTAA
     3901 TGGAGACATC CACTTACTGG AAGTAAGGAG CTGGTAGGCG GCCGCACTCC TCAGGTGCAG
     3961 GCTGCCTATC AGAAGGTGGT GGCTGGTGTG GCCAATGCCC TGGCTCACAA ATACCACTGA
     4021 GATCTTTTTC CCTCTGCCAA AAATTATGGG GACATCATGA AGCCCCTTGA GCATCTGACT
     4081 TCTGGCTAAT AAAGGAAATT TATTTTCATT GCAATAGTGT GTTGGAATTT TTTGTGTCTC
     4141 TCACTCGGAA GGACATATGG GAGGGCAAAT CATTTAAAAC ATCAGAATGA GTATTTGGTT
     4201 TAGAGTTTGG CAACATATGC CATATGCTGG CTGCCATGAA CAAAGGTGGC TATAAAGAGG
     4261 TCATCAGTAT ATGAAACAGC CCCCTGCTGT CCATTCCTTA TTCCATAGAA AAGCCTTGAC
     4321 TTGAGGTTAG ATTTTTTTTA TATTTTGTTT TGTGTTATTT TTTTCTTTAA CATCCCTAAA
     4381 ATTTTCCTTA CATGTTTTAC TAGCCAGATT TTTCCTCCTC TCCTGACTAC TCCCAGTCAT
     4441 AGCTGTCCCT CTTCTCTTAT GAAGATCCCT CGACCTGCAG GCTCCAGCCT ACACAATCGC
     4501 TCAAGACGTG TAATGCTTTT ATTATATATT AGTCACGATA TCTATAACAA GAAAATATAT
     4561 ATATAATAAG TTATCACGTA AGTAGAACAT GAAATAACAA TATAATTATC GTATGAGTTA
     4621 AATCTTAAAA GTCACGTAAA AGATAATCAT GCGTCATTTT GACTCACGCG GTCGTTATAG
     4681 TTCAAAATCA GTGACACTTA CCGCATTGAC AAGCACGCCT CACGGGAGCT CCAAGCGGCG
     4741 ACTGAGATGT CCTAAATGCA CAGCGACGGA TTCGCGCTAT TTAGAAAGAG AGAGCAATAT
     4801 TTCAAGAATG CATGCGTCAA TTTTACGCAG ACTATCTTTC TAGGGTTAAA AGCTTGGCGT
     4861 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA
     4921 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT
     4981 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCGGATCC
     5041 GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC
     5101 TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA
     5161 GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG
     5221 CCTAGGCTTT TGCAAAAAGC TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC
     5281 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG
     5341 TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCCGCT GCATTAATGA ATCGGCCAAC
     5401 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
     5461 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT
     5521 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG
     5581 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG
     5641 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT
     5701 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
     5761 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT
     5821 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC
     5881 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA
     5941 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG
     6001 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG
     6061 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT
     6121 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA
     6181 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC
     6241 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA
     6301 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA
     6361 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT
     6421 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT
     6481 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT
     6541 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT
     6601 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA
     6661 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG
     6721 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT
     6781 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG
     6841 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG
     6901 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC
     6961 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA
     7021 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC
     7081 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT
     7141 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG
     7201 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA
     7261 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA
     7321 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGGGTCG ACTTAACCCT
     7381 AGAAAGATAA TCATATTGTG ACGTACGTTA AAGATAATCA TG
//