LOCUS       pAAV_CAG_DIO_ChR        7311 bp ds-DNA     circular     22-JAN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CAG-DIO(ChR_25_9_TS_YFP)-WPRE-pA (R03)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Aug 12, 2018 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     LOCUS pAAV-EF1a-DIO-hC 7266 bp DNA circular 04-MAR-2016 SOURCE
            ORGANISM KEYWORDS COMMENT 
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label=L-ITR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        164..1263
                     /note="CAG"
                     /label=CAG
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1303..1336
                     /label=lox2722
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1387..1420
                     /label=loxP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(1432..2148)
                     /codon_start=1
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     K
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     G
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     V
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     E FVTAAGITLGMDELYK"
                     /label=EYFP (no start)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2149..2208
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0 ApEinfo_revcolor=green"
                     /label=TS
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(2218..3285)
                     /codon_start=1
                     /translation="MSRLVAASWLLALLLCGITSTTTASAHIVMVDAYKPTKSAPAAS
                     S
                     TDGTAAAAVSHYAMNGFDELAKGAVVPEDHFVCGPADKCYCSAWLHSRGALWEQETAR
                     G
                     FQWFAVFLSALFLAFYGWHAYKASVGWEEVYVCSVELIKVILEIYFEFTSPAMLFLYG
                     G
                     NITPWLRYAEWLLTCPVILIHLSNITGLSEAYNKRTMALLVSDLGTICMGVTAALATG
                     W
                     VKWLFYCIGLVYGTQTFYNAGIIYVEAYHTVPKGRCRQVVTGMAWLFFVSWGMFPILF
                     I
                     LGPEGFGVLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKIHEHIIMYGDIRRPVSSQF
                     L GRKVDVLAFVTEEDKV"
                     /label=ChRger2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3296..3329)
                     /label=lox2722(1)
                     /ApEinfo_label=lox2722
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3380..3413)
                     /label=loxP(1)
                     /ApEinfo_label=loxP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3428..4036
                     /label=WPRE
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     polyA_signal    4056..4534
                     /label=hGH polyA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4574..4714
                     /label=R-ITR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      4806..5112
                     /direction=RIGHT
                     /label=f1 Origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5631..6488
                     /label=AmpR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      6639..7306
                     /direction=RIGHT
                     /label=pUC ori
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaacac tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
      541 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
      601 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
      661 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
      721 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
      781 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
      841 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
      901 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
      961 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagcgccgg caggaaggaa
     1021 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     1081 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1141 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     1201 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     1261 ttgcggccca acggtaccgg atcctctaga gtcgactccg gaataacttc gtataggata
     1321 ctttatacga agttatgcag aatggtagct ggattgtagc tgctattagc aatatgaaac
     1381 ctcttaataa cttcgtatag catacattat acgaagttat ggcgcgccct attacttgta
     1441 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
     1501 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg tagctcaggt agtggttgtc
     1561 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
     1621 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
     1681 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
     1741 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
     1801 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
     1861 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     1921 gtcggggtag cgggcgaagc actgcaggcc gtagccgaag gtggtcacga gggtgggcca
     1981 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     2041 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     2101 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca cgttgatgtc
     2161 gatctggtcc agggggatgt actcgccctc gctggtgatc ctgctcttgg cggccgccac
     2221 tttatcctcc tctgtcacga aggccaggac gtccaccttg cggcccagaa actgggagct
     2281 cactggtctg cgaatgtcgc cgtacataat gatatgctcg tggattttga tgcgcaggaa
     2341 gtgcccgagc agtccccaaa tattcttgga cagcaggtcg gcgatggtgt ggccaatagt
     2401 ggacccagcc acgctcagga cgccgaagcc ctcggggccg aggatgaaca ggatggggaa
     2461 cataccccat gatacgaaga agagccaagc catgccagtc accacctggc gacaccggcc
     2521 cttcggcacg gtgtggtacg cctccacgta gatgattcct gcgttgtaga atgtctgggt
     2581 gccgtacacc aggccgatac agtaaaacag ccacttcacc cacccagtgg ccagagcggc
     2641 tgtcacgccc atgcagatag tgcccaggtc tgacaccagc agagccatgg tacgcttatt
     2701 atatgcctca ctcagaccgg tgatgttgct cagatggata agaatgacag ggcatgtcag
     2761 cagccactcg gcgtaacgaa gccatggggt aatgttgcct ccgtacagga acagcatcgc
     2821 aggactggta aactcgaaat agatctccag aatcactttg atcagctcca cggagcacac
     2881 gtacacctcc tcccagccca cgctggcctt ataggcgtgc cagccgtaga aagccagaaa
     2941 cagggcagac aggaacacgg caaaccactg gaagcccctg gctgtttcct gctcccacag
     3001 ggctcctctg ctgtgcagcc aagcggagca atagcacttg tcggctggtc cgcagacaaa
     3061 gtggtcttct ggcaccacgg ctcctttagc cagctcgtcg aagccgttca tggcgtagtg
     3121 agacactgct gcggcggctg ttccgtctgt agaagaagct gctggggcgc tcttggtggg
     3181 cttgtaggcg tccaccatca cgatgtgggc agaggctgtt gttgtgctgg taattccgca
     3241 gaggaggaga gccagcagcc aagaagcggc gaccagtctg ctcatggtgg ctagcataac
     3301 ttcgtataaa gtatcctata cgaagttatt tgccttaacc cagaaattat cactgttatt
     3361 ctttagaatg gtgcaaagaa taacttcgta taatgtatgc tatacgaagt tatgaattcg
     3421 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     3481 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     3541 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     3601 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     3661 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     3721 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     3781 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     3841 cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct
     3901 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     3961 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4021 ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc
     4081 cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat
     4141 aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag
     4201 gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta
     4261 ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg
     4321 ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac
     4381 caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct
     4441 ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt
     4501 acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg
     4561 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     4621 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     4681 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
     4741 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     4801 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4861 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4921 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4981 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     5041 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5101 aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc
     5161 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     5221 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     5281 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     5341 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     5401 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     5461 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     5521 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5581 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5641 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5701 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5761 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5821 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5881 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5941 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     6001 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     6061 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     6121 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     6181 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     6241 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     6301 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     6361 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     6421 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6481 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     6541 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6601 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6661 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6721 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6781 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     6841 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6901 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6961 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7021 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7081 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7141 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7201 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7261 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
