LOCUS pAAV_CAG_DIO_ChR 7326 bp ds-DNA circular 22-JAN-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAAV-CAG-DIO(ChR_11_10_TS_YFP)-WPRE-pA (R03) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7326) AUTHORS . TITLE Direct Submission JOURNAL Exported Sunday, Aug 12, 2018 from SnapGene 3.3.4 http://www.snapgene.com COMMENT LOCUS pAAV-EF1a-DIO-hC 7266 bp DNA circular 04-MAR-2016 SOURCE ORGANISM KEYWORDS COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 1..141 /label=L-ITR /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 164..1263 /note="CAG" /label=CAG /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1303..1336 /label=lox2722 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1387..1420 /label=loxP /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(1432..2148) /codon_start=1 /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL K FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD G NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK V NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL E FVTAAGITLGMDELYK" /label=EYFP (no start) /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2149..2208 /note="ApEinfo_fwdcolor=#00ff00 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ApEinfo_revcolor=green" /label=TS /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(2218..3300) /codon_start=1 /translation="MSRRPWLLALALAVALAAGSAGAAHIVMVDAYKPTKSTGSDATV P VATQDGPDYVFHRAHERMLFQTSYTLENNGSVICIPNNGQCFCLAWLKSNGTNAEKLA A NILQWITFALSALCLMFYGYQTWKSTCGWEEIYVATIEMIKFIIEYFHEFDSPAMLFL Y GGNITPWLRYAEWLLTCPVILIHLSNITGLSEAYNKRTMALLVSDLGTICMGVTAALA T GWVKWLFYCIGLVYGTQTFYNAGIIYVEAYHTVPKGRCRQVVTGMAWLFFVSWGMFPI L FILGPEGFGVLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKIHEHILIHGDIRKTTKL N IGGTEIEVETLVEDEAEAGAV" /label=ChRger3 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(3311..3344) /label=lox2722(1) /ApEinfo_label=lox2722 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(3395..3428) /label=loxP(1) /ApEinfo_label=loxP /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3443..4051 /label=WPRE /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 polyA_signal 4071..4549 /label=hGH polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4589..4729 /label=R-ITR /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 4821..5127 /direction=RIGHT /label=f1 Origin /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5646..6503 /label=AmpR /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 6654..7321 /direction=RIGHT /label=pUC ori /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtaacac tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 301 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 481 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca 541 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta 601 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg 661 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc 721 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata 781 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct 841 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga 901 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg 961 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagcgccgg caggaaggaa 1021 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct 1081 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc 1141 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt 1201 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa 1261 ttgcggccca acggtaccgg atcctctaga gtcgactccg gaataacttc gtataggata 1321 ctttatacga agttatgcag aatggtagct ggattgtagc tgctattagc aatatgaaac 1381 ctcttaataa cttcgtatag catacattat acgaagttat ggcgcgccct attacttgta 1441 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg 1501 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg tagctcaggt agtggttgtc 1561 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg 1621 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg 1681 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag 1741 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc 1801 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt 1861 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg 1921 gtcggggtag cgggcgaagc actgcaggcc gtagccgaag gtggtcacga gggtgggcca 1981 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc 2041 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc 2101 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca cgttgatgtc 2161 gatctggtcc agggggatgt actcgccctc gctggtgatc ctgctcttgg cggccgcgac 2221 cgcgccagcc tcggcctcgt cctccaccag cgtctcgacc tcaatctcag tgccaccaat 2281 gttcaatttg gtggtcttgc gaatgtcgcc gtggatgagg atatgctcgt ggattttgat 2341 gcgcaggaag tgcccgagca gtccccaaat attcttggac agcaggtcgg cgatggtgtg 2401 gccaatagtg gacccagcca cgctcaggac gccgaagccc tcggggccga ggatgaacag 2461 gatggggaac ataccccatg atacgaagaa gagccaagcc atgccagtca ccacctggcg 2521 acaccggccc ttcggcacgg tgtggtacgc ctccacgtag atgattcctg cgttgtagaa 2581 tgtctgggtg ccgtacacca ggccgataca gtaaaacagc cacttcaccc acccagtggc 2641 cagagcggct gtcacgccca tgcagatagt gcccaggtct gacaccagca gagccatggt 2701 acgcttatta tatgcctcac tcagaccggt gatgttgctc agatggataa gaatgacagg 2761 gcatgtcagc agccactcgg cgtaacgaag ccatggggta atgttgcctc cgtacaggaa 2821 cagcatcgca ggactgtcaa actcatggaa atactcgatg atgaacttga tcatctcgat 2881 cgtggccacg taaatctcct cccagccgca agtagacttc caggtctggt agccgtagaa 2941 catcaggcag agcgctgaaa gcgcaaaagt aatccactgc agaatgttgg cagccaactt 3001 ctcggcattt gttccgttgg atttaagcca agccaagcag aagcactggc cgttgttcgg 3061 gatgcaaata acagaaccat tgttctcaag agtgtatgag gtttggaaga gcatgcgctc 3121 gtgggcacgg tggaaaacgt agtcggggcc atcctgagtc gcgaccggca ccgtcgcgtc 3181 actgccagtc gacttggtgg gcttgtaggc gtccaccatc acgatgtggg cggctcctgc 3241 gctgccggcc gccagcgcca ctgccagcgc tagggcaaga agccatggcc tccgcgacat 3301 ggtggctagc ataacttcgt ataaagtatc ctatacgaag ttatttgcct taacccagaa 3361 attatcactg ttattcttta gaatggtgca aagaataact tcgtataatg tatgctatac 3421 gaagttatga attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg 3481 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 3541 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 3601 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 3661 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 3721 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 3781 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 3841 cggggaaatc atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg 3901 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 3961 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 4021 ccctttgggc cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca 4081 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac 4141 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa 4201 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag 4261 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc 4321 aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt 4381 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac 4441 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc 4501 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta 4561 ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc 4621 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc 4681 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg 4741 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat 4801 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 4861 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 4921 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 4981 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg 5041 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 5101 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt 5161 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 5221 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa 5281 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 5341 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 5401 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 5461 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 5521 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 5581 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 5641 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 5701 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 5761 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 5821 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 5881 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 5941 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 6001 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 6061 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 6121 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 6181 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 6241 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 6301 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 6361 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 6421 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 6481 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 6541 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 6601 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 6661 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 6721 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 6781 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 6841 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 6901 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 6961 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 7021 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 7081 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 7141 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 7201 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 7261 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 7321 acatgt //