LOCUS       Exported               13304 bp ds-DNA     circular SYN 02-MAY-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    PBNeo_TetO_Cdk13_isoform1NT
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 13304)
  AUTHORS   Sara Dubbury
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 2, 2019 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13304
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    600..615
                     /label=M13F
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     LTR             complement(746..946)
                     /label=pBac 3'- LTR
                     /label=pBac\3'-LTR
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=19"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    complement(1021..1246)
                     /label=BGH polyA
                     /label=BGH\polyA
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=21"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    1278..1283
                     /label=XbaI
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     primer_bind     complement(1297..1305)
                     /label=stop
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=28"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    complement(1306..1347)
                     /label=V5
                     /note="ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff00ff
                     vntifkey=21"
                     /note="color: #ff00ff; direction: LEFT"
     protein_bind    1349..1373
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    complement(1350..1356)
                     /label=attR2
                     /note="ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff00ff
                     vntifkey=21"
                     /note="color: #ff00ff; direction: LEFT"
     misc_feature    1387..1413
                     /note="SJD36_CDK13_R_XhoI_R+Stop"
                     /note="color: #50ff50; direction: RIGHT"
     CDS             complement(1393..5928)
                     /codon_start=1
                     /label=Cdk13 Transgene
                     /note="color: #ff0000"
                     /translation="MPSSSDTALGGGGGLSWAEKKLEERRKRRRFLSPQQPPLLLPLLQ
                     PQLLQPPPPPPPLLFLAAPGAAAAAAAAAAASSSCFSPGPPLEVKRLARGKRRPGGRQK
                     RRRGPRAGQEAEKRRVFSLPQPQQDGGGGASSGGGVTPLVEYEDVSSQSEQGLLLGGAS
                     AATAATAAGGTGGNGGSPASSSGTQRRAEGSERRPRRDRRSSSGRSKERHREHRRRDGT
                     RSGSEASKARSRHGHSGEERAEAAKSGSSSSSGGRRKSASATSSSSSSRKDRDLKAHRS
                     RTKSSKEPPSAYKEPPKAYREDKSEPKAYRRRQRSLSPLGGRDESPVSHRASQSLRSRK
                     SPSPAGGGSSPYSRRLPRSPSPYSRRRSPSYSRHSSYERGGDVSPSPYSSSSWRRSRSP
                     YSPVLRRSAKSRSRSPYSSRHSRSRSRHRLSRSRSRHSSISPSTLTLKSSLAAELNKNK
                     KARAAEAARAAEAAKAAEAAKAAEAAAKAAKASNASTPTKGNTETGASVSQTNHVKEVK
                     KLKTEHAPSPSSGGTVKSDKAKTKPPLQVTKVDNNLTVEKATKKTVVGKESKPAATKEE
                     PVSTKEKSKPLTPSTGAKEKEQHVALVTSTLPPLPLPPMLPEDKDADSLRGNISVKAVK
                     KEVEKKLRCLLADLPLPPELPGGDDLSKSPEEKKTAAQLHSKRRPKICGPRYGEIKEKD
                     IDWGKRCVDKFDIIGIIGEGTYGQVYKARDKDTGEMVALKKVRLDNEKEGFPITAIREI
                     KILRQLTHQSIINMKEIVTDKEDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFNEN
                     HIKSFMRQLMEGLDYCHKKNFLHRDIKCSNILLNNRGQIKLADFGLARLYSSEESRPYT
                     NKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIFQANQELAQLELISRIC
                     GSPCPAVWPDVIKLPYFNTMKPKKQYRRKLREEFVFIPAAALDLFDYMLALDPSKRCTA
                     EQALQCEFLRDVEPSKMPPPDLPLWQDCHELWSKKRRRQKQMGMTDDLSTIKAPRKDLS
                     LGLDDSRTNTPQGVLPPAQLKSQSNSNVAPVITGPGQPLNHSELAILLNLLQSKSSVNM
                     ADFVQVLNIKVNSETQQQLNKINLPAGILATGEKQTDPSTPQQESSKSLGGVQPSQTIQ
                     PKVETDAAQAAVQSAFAVLLTQLIKAQQSKQKDAMLEERENGSGHEAPLQLRPPLEPST
                     PGSGQDDLIQHQDRRILELTPEPDRPRILPPDQRPPEPPEPPPVTEEDLDYRTENQHVP
                     TTSSSLTDPHAGVKAALLQLLAQHQPQDDPKREGGIDYPTGDTYVPSSDYKDNFGSSFS
                     AAPYVSSDGLGSSSAAAPLEARSFIGNSDIQSLDNYSTASSHTGGPPQTSAFTESFASS
                     VAGYGDIYLNAGPMLFSGDKDHRFEYSHGPITVLTNSNDPSTGPESTHPLPAKMHNYNY
                     GGNLQENPGGPSLMHGQTWTSPAQGPGYSQGYRGHISTSAGRGRGRGLPY"
     misc_feature    1754..1774
                     /note="SJD25"
                     /note="color: #50ff50"
     misc_feature    2406..2427
                     /note="SJD24"
                     /note="color: #50ff50"
     misc_feature    3174..3195
                     /note="SJD23"
                     /note="color: #50ff50"
     misc_feature    3930..3951
                     /note="SJD22"
                     /note="color: #50ff50"
     misc_feature    4155..4173
                     /note="SJD167"
                     /note="color: #50ff50; direction: RIGHT"
     misc_feature    4375..5949
                     /note="Genewiz 5' Optimized"
                     /note="color: #0080ff"
     misc_feature    5638..5659
                     /note="SJD144"
                     /note="color: #50ff50"
     misc_feature    5832..5852
                     /note="SJD159"
                     /note="color: #50ff50; direction: RIGHT"
     misc_feature    5906..5949
                     /note="SJD107_KpnI-strKozakATG-opt5'CDK13_F"
                     /note="color: #50ff50"
     misc_feature    complement(5926..5928)
                     /label=StartCodon
                     /note="color: #339966; direction: LEFT"
     protein_bind    complement(5964..5988)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     misc_feature    complement(5974..5988)
                     /label=attR1
                     /note="ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff00ff
                     vntifkey=21"
                     /note="color: #ff00ff; direction: LEFT"
     misc_feature    5999..6004
                     /label=SpeI
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     misc_feature    complement(6032..6469)
                     /label=TetO
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#00ff00
                     vntifkey=21"
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    complement(6470..6475)
                     /label=XhoI
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    complement(6476)
                     /label=LoxP_mutation
                     /note="lox-P site
                     ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff00ff
                     vntifkey=21"
                     /note="color: #ff00ff; direction: LEFT"
     misc_feature    complement(6478..6674)
                     /label=bpA
                     /note="bovine growth hormone polyA_site
                     ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff00ff
                     vntifkey=21"
                     /note="color: #ff00ff; direction: LEFT"
     CDS             complement(6713..7516)
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /label=neo
                     /note="ApEinfo_fwdcolor=#0000ff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#0000ff
                     vntifkey=4"
                     /note="color: #0000ff"
                     /db_xref="GI:13492216"
                     /protein_id="AAK28133.1"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(7525..7850)
                     /label=pgk(1)
                     /note="PGK
                     ApEinfo_fwdcolor=#80ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=pgk
                     ApEinfo_revcolor=#999999
                     vntifkey=29"
                     /note="color: #999999; direction: LEFT"
     promoter        complement(7851..8020)
                     /label=pgk
                     /note="PGK
                     ApEinfo_fwdcolor=#80ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#999999
                     vntifkey=29"
                     /note="color: #999999; direction: LEFT"
     intron          complement(7851..8020)
                     /label=sequenced
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=15"
                     /note="color: #ffc0cb; direction: LEFT"
     promoter        complement(8021..8301)
                     /label=SV40 promoter /origin(1) promoter
                     /label=SV40\promoter/origin(1)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=SV40\promoter/origin
                     ApEinfo_revcolor=pink
                     vntifkey=30"
                     /note="color: #ffc0cb; direction: LEFT"
     promoter        complement(8302..8340)
                     /label=SV40 promoter /origin promoter
                     /label=SV40\promoter/origin
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=30"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_binding    8341..8360
                     /label=HIV flap/nuclear import signal
                     /label=HIV\flap/nuclear\import\signal
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=20"
                     /note="color: #ffc0cb"
     misc_feature    join(8363,8368..8372)
                     /label=TetO(1)
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=TetO
                     ApEinfo_revcolor=#00ff00
                     vntifkey=21"
                     /note="This feature has 2 segments:
                        1: 8363 .. 8363 / #00ff00
                        2: 8368 .. 8372 / #00ff00"
     misc_feature    8382..8386
                     /label=ClaI
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     misc_feature    join(8395..8400,8411..8459)
                     /label=UpdatedBarcodeFragment
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="This feature has 2 segments:
                        1: 8395 .. 8400 / #00ffff
                        2: 8411 .. 8459 / #00ffff"
     misc_feature    join(8395..8400,8411..8419)
                     /label=HW146
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="This feature has 2 segments:
                        1: 8395 .. 8400 / #00ffff
                        2: 8411 .. 8419 / #00ffff"
     misc_feature    complement(8424..8449)
                     /label=HW147
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: LEFT"
     LTR             8463..8768
                     /label=pBac 5'- LTR
                     /label=pBac\5'-LTR
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=19"
                     /note="color: #ffc0cb"
     misc_feature    complement(9261..9277)
                     /label=M13R
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    9602..11501
                     /label=CEN-URA3
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ff00"
     misc_feature    11540..12221
                     /label=colEI ori
                     /note="ApEinfo_fwdcolor=#c0c0c0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #c0c0c0"
     misc_feature    12318..13175
                     /label=Amp
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg
      661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
      721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa
      781 agataatcat gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
      841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
      901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct
      961 tctataaagt aacaaaactt ttatcgaatt cctgcagccc agcttgatcc cgcctcagaa
     1021 gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
     1081 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     1141 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     1201 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
     1261 cgggtttaaa cgggccctct agaggttagc gctagctcat tactacgtag aatcgagacc
     1321 gaggagaggg ttagggatag gcttaccaac cactttgtac aagaaagctg ggtcgaattc
     1381 gcccttctcg agtcagtatg gtaaccctct gcctcgacct ctgccagctg atgtgctaat
     1441 gtgtcccctg tatccttgtg aatatccagg tccttgggca ggagaagtcc aggtctgtcc
     1501 atgcatgagg ctagggccac ctggattttc ctgtaagtta ccaccatagt tatagttgtg
     1561 catctttgct ggcaagggat gagtactctc tggccctgtg gaagggtcat tgctgtttgt
     1621 gaggactgtg ataggaccat ggctatattc aaatctatgg tccttgtctc cactaaacaa
     1681 catgggacca gcattgaggt aaatgtctcc atatccagct actgaactgg caaacgactc
     1741 agtaaaggca gaagtttgag gtggaccacc agtgtgagat gaagcagtac tgtagttatc
     1801 cagagactga atatctgagt ttccaatgaa actacgtgct tccaatggtg cagcagcgga
     1861 gctacttcct agaccatcac tgctaacgta aggagcggca gagaaagaag atccaaagtt
     1921 gtccttatag tctgaactgg gcacatatgt gtctcctgtg ggataatcga taccaccttc
     1981 tcttttggga tcatcctggg gctgatgctg agcaagcagc tgtaagaggg ctgccttcac
     2041 tccagcatgt gggtcagtta atgaagaact agtggtagga acatgctggt tttctgtccg
     2101 ataatctagg tcctcctcag taactggtgg tggttcagga ggttcaggag gccgttgatc
     2161 aggaggcaga attcgaggcc ggtctggttc tggtgtcagc tccaatatcc tcctgtcttg
     2221 gtgctggatg aggtcatctt gcccggatcc aggagtgctc ggttctagag gaggcctgag
     2281 ttgcaatgga gcttcatgtc ctgatccatt ttccctttcc tctagcatgg catctttctg
     2341 tttggactgt tgggccttta ttaactgagt caagaggact gcaaatgcac tctgcacagc
     2401 agcctgggca gcatcagttt ccactttagg ctggatggtc tgtgaaggct gaactcctcc
     2461 caatgatttt gaagactcct gttgtggtgt tgatggatct gtctgttttt cacctgttgc
     2521 caaaattcca gcaggaaggt ttattttatt tagctgctgt tgagtctcag agtttacctt
     2581 aatgttcaac acttggacaa aatcagccat attaacactt gatttagatt gtagtaggtt
     2641 tagaagaatt gccaattcac tgtggtttaa cggctgtcca gggcctgtta ttacaggtgc
     2701 tacatttgag ttgctctgag atttcaactg tgcgggtggc agcacaccct ggggtgtgtt
     2761 agttctgctg tcatctaagc ccagagacag gtccttccta ggggctttga ttgtggaaag
     2821 atcatcagtc atgcccatct gtttctgtct tcttctcttt ttactccata attcatgaca
     2881 atcttgccac aaaggaaggt ctggtggagg cattttggag ggttccacgt ctcgcaggaa
     2941 ctcacactga agagcctgct cagcagtgca gcgcttactg ggatccaagg caagcatgta
     3001 atcgaataag tcgagtgcag ctgcggggat gaaaacaaat tcttctctta acttccgccg
     3061 atattgcttc tttggtttca tggtgttgaa atatggcagt ttgattacat caggccacac
     3121 tgcaggacat ggactcccac atatacggct tattagctct agctgtgcaa gttcctggtt
     3181 tgcttgaaat ataggttttt tagtgaagag ttcaccaagg atacatccac agctccatac
     3241 atcaatggct ggtgtatatc gttcttctcc caagagcaat tcaggtggac gataccacaa
     3301 agtaatgacc ttgttagtat atgggcgact ttcttctgag ctatataaac gagcaagtcc
     3361 aaaatctgca agttttatct gtcctctatt atttagaagg atatttgaac atttaatatc
     3421 tctatgcaaa aagttcttct tatgacaata atccaggcct tccatgagct gtctcataaa
     3481 agattttata tggttttcat taaaatgaac caagcctgat tccagcagtc ccatcagatc
     3541 atggtccata tattcaaaca ccaggtaaaa tgcacctttg tctttcttaa aatccaaagc
     3601 atcttcttta tcagtcacga tttccttcat attgatgata ctctggtggg tgagttgccg
     3661 aagaatttta atttctctaa ttgctgtaat tgggaaaccc tccttttcat tatccagacg
     3721 tactttcttt aaggctacca tttctcccgt gtctttgtcc ctggctttgt aaacttgtcc
     3781 ataagtacct tctccaataa ttccgatgat atcaaattta tccacgcagc gtttccccca
     3841 gtcaatatct ttttctttga tttcaccata gcgaggccca catattttag gccttcgttt
     3901 gctatgtaac tgtgctgctg ttttcttctc ctctggactc ttggaaagat catctcctcc
     3961 tggtaactca gggggcaatg gtaaatcagc aagcagacat cggagtttct tttctacttc
     4021 ttttttaact gctttgacag aaatgttgcc tcttaagcta tcagcatctt tatcttcagg
     4081 cagcatggga ggcaaaggta atggcggtaa cgtagaggtc actaaagcca catgctgctc
     4141 cttctccttg gctcctgtgc ttggtgtgag tggcttgctt ttctctttag tggaaactgg
     4201 ttcttccttt gtagcagcag gtttactctc cttcccaaca actgttttct tggtggcttt
     4261 ctctactgtc aaattattgt ctacctttgt tacttgaagc ggtggctttg tttttgcttt
     4321 gtcgcttttg acggtcccac cacttgaagg agaaggtgca tgctcagttt taagcttctt
     4381 gacttccttc acgtggttgg tctgggagac ggaggctccg gtctcggtgt ttcccttggt
     4441 gggtgtggaa gcgttggagg ccttagcagc cttggcggcg gcttcagcag ctttggcggc
     4501 ttcggcggct ttagcggctt cggcagctct agcagcctca gcggccctag ctttcttgtt
     4561 cttgttgagc tcggcggcca gggagctctt cagtgtcagg gtggaggggc tgatggagga
     4621 gtgcctgctt ctggacctgc tcagcctgtg cctggacctg cttctgctgt gcctggagga
     4681 gtaagggctc ctggacctgg acttggcgga cctcctcagc acaggggaat agggggatct
     4741 gctcctcctc caggaggagg agctgtaggg ggagggggag acatctcctc ctctctcgta
     4801 ggaggaatgc ctgctgtagc tgggggatct cctcctgctg taggggctag gggacctggg
     4861 cagtcttctg gaataagggc tgcttccgcc tccagcgggg gaagggcttt tcctggatct
     4921 cagggactgg ctagccctgt ggctgacagg ggactcgtct ctgcctccga gagggctgag
     4981 ggacctctgc ctcctcctgt aggccttggg ttcggacttg tcttccctgt aggctttggg
     5041 gggctccttg taggcggaag ggggttcctt ggagctcttg gtcctgctcc tatgagcctt
     5101 gaggtccctg tccttcctgg agctgctgct ggaggatgtg gcggaggcgg actttcttct
     5161 tccgccggag gagctggagg agccggattt ggcagcctcg gctctttcct cgccgctgtg
     5221 tccgtgtctg gatctggcct tgctggcctc ggatccgctc cttgtgccat ctctcctcct
     5281 atgttccctg tgcctctctt tggacctgcc gctggagctc cttctgtccc ttctaggtct
     5341 tctctcgctg ccctcggctc ttctctgggt tccgctgctg gaagcggggg atcctccatt
     5401 tcctccggtt cctccagcag cggtggcagc tgtagcagcg ctagctcctc ccaggagcag
     5461 gccttgttcg ctttggctgg acacgtcctc gtactccacc aggggtgtca ctcctcctcc
     5521 gctggaagct cctccgcctc catcctgctg gggttgaggc agggagaaca ctctcctctt
     5581 ttcggcttcc tgtccggccc taggtcctct ccttctcttt tgcctgccgc cgggtctcct
     5641 ttttcctctg gccagcctct tgacctccag aggagggcca gggctaaaac aggagctgga
     5701 agcggcagca gcagcggcgg cagcggcagc ggctccagga gcagccagaa acaggagagg
     5761 agggggagga ggagggggtt ggaggagctg aggttgcagg agagggagca gcagaggagg
     5821 ctgctgaggg ctgagaaacc tcctcctctt cctcctctcc tccagctttt tctcggccca
     5881 gctgagtccg cctcctccgc cgagagctgt atcggagctg gaaggcatgg tggcggcggt
     5941 accagtagta agggcgaatt cggagcctgc ttttttgtac aaacttgttg atatctgcac
     6001 tagtggatcc gagctcggta cgaattcggg gccgcggagg ctggatcggt cccggtgtct
     6061 tctatggagg tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg
     6121 agctctgctt atataggcct cccaccgtac acgcctactc gacccgggta ccgagctcga
     6181 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
     6241 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
     6301 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
     6361 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
     6421 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgagttgcc
     6481 tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag
     6541 aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg
     6601 gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc
     6661 aactagaagg cacagtcgag gctgatcagc gagctctaga gaattgatcc cctcagaaga
     6721 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
     6781 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
     6841 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
     6901 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat
     6961 cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
     7021 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
     7081 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
     7141 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
     7201 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
     7261 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
     7321 cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
     7381 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
     7441 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
     7501 caatggccga tcccatattg gctgcaggtc gaaaggcccg gagatgagga agaggagaac
     7561 agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg ggcctccgga ggaccttcgg
     7621 gcgcccgccc cgcccctgag cccgcccctg agcccgcccc cggacccacc ccttcccagc
     7681 ctctgagccc agaaagcgaa ggagcaaagc tgctattggc cgctgcccca aaggcctacc
     7741 cgcttccatt gctcagcggt gctgtccatc tgcacgagac tagtgagacg tgctacttcc
     7801 atttgtcacg tcctgcacga cgcgagctgc ggggcggggg ggaacttcct gactagggga
     7861 ggagtagaag gtggcgcgaa ggggccacca aagaacggag ccggttggcg cctaccggtg
     7921 gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc gccaagtgcc cagcggggct
     7981 gctaaagcgc atgctccaga ctgccttggg aaaagtactg cctaggcctc caaaaaagcc
     8041 tcctcactac ttctggaata gctcagaggc agaggcggcc tcggcctctg cataaataaa
     8101 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     8161 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     8221 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     8281 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     8341 acagcagaga tccagtttgg ttagatctcg aggtcgacgg tatcgttggc cggtactcga
     8401 gaagcgtatg agatcggaag agcggttcag caggaatgcc gagaccgcgc caacgataag
     8461 ctatctataa caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa
     8521 caatataatt atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat
     8581 tttgactcac gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg
     8641 cctcacggga gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc
     8701 tatttagaaa gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct
     8761 ttctagggtt aatctagctg catcaggatc atatcgtcgg gtcttttttc aattgctcag
     8821 tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg ccttttcccg
     8881 cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca ttgacgttga
     8941 gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg
     9001 tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc ggacacagtt
     9061 ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca gccggaactg
     9121 ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg tcagcgagga
     9181 cgggtatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt tacccggcgg
     9241 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     9301 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     9361 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9421 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     9481 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9541 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9601 acctgggtcc ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa
     9661 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt
     9721 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt
     9781 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg
     9841 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc
     9901 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt
     9961 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat
    10021 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt
    10081 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcctaagaa accattatta
    10141 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
    10201 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
    10261 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    10321 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac cacgcttttc
    10381 aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttctt tgaaattttt
    10441 ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg
    10501 tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac
    10561 tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg
    10621 aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa
    10681 agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag
    10741 ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt
    10801 tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac
    10861 tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg
    10921 gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag
    10981 gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt
    11041 tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta
    11101 ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca
    11161 tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg
    11221 acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat
    11281 ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg
    11341 gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact
    11401 aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt
    11461 aattatatca gttattaccc tgcggtgtga aataccgcac agagcaaaag gccagcaaaa
    11521 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    11581 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    11641 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    11701 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    11761 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    11821 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    11881 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    11941 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    12001 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    12061 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    12121 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    12181 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    12241 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    12301 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    12361 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    12421 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    12481 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    12541 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    12601 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    12661 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    12721 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    12781 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    12841 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    12901 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    12961 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    13021 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    13081 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    13141 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    13201 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    13261 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//
