LOCUS       Exported               13355 bp ds-DNA     circular SYN 02-MAY-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    PBNeo_TetO_Cdk13_isoform1NFH
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 13355)
  AUTHORS   Sara Dubbury
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, May 2, 2019 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13355
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    600..615
                     /label=M13F
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     LTR             complement(746..946)
                     /label=pBac 3'- LTR
                     /label=pBac\3'-LTR
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=19"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    complement(1021..1246)
                     /label=BGH polyA
                     /label=BGH\polyA
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=21"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    1278..1283
                     /label=XbaI
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     primer_bind     complement(1297..1305)
                     /label=stop
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=28"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    complement(1306..1347)
                     /label=V5
                     /note="ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff00ff
                     vntifkey=21"
                     /note="color: #ff00ff; direction: LEFT"
     protein_bind    1349..1373
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    complement(1350..1356)
                     /label=attR2
                     /note="ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff00ff
                     vntifkey=21"
                     /note="color: #ff00ff; direction: LEFT"
     misc_feature    1387..1413
                     /note="SJD36_CDK13_R_XhoI_R+Stop"
                     /note="color: #50ff50; direction: RIGHT"
     CDS             complement(1396..5925)
                     /codon_start=1
                     /label=Cdk13_isoform1
                     /note="color: #993366"
                     /translation="PSSSDTALGGGGGLSWAEKKLEERRKRRRFLSPQQPPLLLPLLQP
                     QLLQPPPPPPPLLFLAAPGAAAAAAAAAAASSSCFSPGPPLEVKRLARGKRRPGGRQKR
                     RRGPRAGQEAEKRRVFSLPQPQQDGGGGASSGGGVTPLVEYEDVSSQSEQGLLLGGASA
                     ATAATAAGGTGGNGGSPASSSGTQRRAEGSERRPRRDRRSSSGRSKERHREHRRRDGTR
                     SGSEASKARSRHGHSGEERAEAAKSGSSSSSGGRRKSASATSSSSSSRKDRDLKAHRSR
                     TKSSKEPPSAYKEPPKAYREDKSEPKAYRRRQRSLSPLGGRDESPVSHRASQSLRSRKS
                     PSPAGGGSSPYSRRLPRSPSPYSRRRSPSYSRHSSYERGGDVSPSPYSSSSWRRSRSPY
                     SPVLRRSAKSRSRSPYSSRHSRSRSRHRLSRSRSRHSSISPSTLTLKSSLAAELNKNKK
                     ARAAEAARAAEAAKAAEAAKAAEAAAKAAKASNASTPTKGNTETGASVSQTNHVKEVKK
                     LKTEHAPSPSSGGTVKSDKAKTKPPLQVTKVDNNLTVEKATKKTVVGKESKPAATKEEP
                     VSTKEKSKPLTPSTGAKEKEQHVALVTSTLPPLPLPPMLPEDKDADSLRGNISVKAVKK
                     EVEKKLRCLLADLPLPPELPGGDDLSKSPEEKKTAAQLHSKRRPKICGPRYGEIKEKDI
                     DWGKRCVDKFDIIGIIGEGTYGQVYKARDKDTGEMVALKKVRLDNEKEGFPITAIREIK
                     ILRQLTHQSIINMKEIVTDKEDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFNENH
                     IKSFMRQLMEGLDYCHKKNFLHRDIKCSNILLNNRGQIKLADFGLARLYSSEESRPYTN
                     KVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIFQANQELAQLELISRICG
                     SPCPAVWPDVIKLPYFNTMKPKKQYRRKLREEFVFIPAAALDLFDYMLALDPSKRCTAE
                     QALQCEFLRDVEPSKMPPPDLPLWQDCHELWSKKRRRQKQMGMTDDLSTIKAPRKDLSL
                     GLDDSRTNTPQGVLPPAQLKSQSNSNVAPVITGPGQPLNHSELAILLNLLQSKSSVNMA
                     DFVQVLNIKVNSETQQQLNKINLPAGILATGEKQTDPSTPQQESSKSLGGVQPSQTIQP
                     KVETDAAQAAVQSAFAVLLTQLIKAQQSKQKDAMLEERENGSGHEAPLQLRPPLEPSTP
                     GSGQDDLIQHQDRRILELTPEPDRPRILPPDQRPPEPPEPPPVTEEDLDYRTENQHVPT
                     TSSSLTDPHAGVKAALLQLLAQHQPQDDPKREGGIDYPTGDTYVPSSDYKDNFGSSFSA
                     APYVSSDGLGSSSAAAPLEARSFIGNSDIQSLDNYSTASSHTGGPPQTSAFTESFASSV
                     AGYGDIYLNAGPMLFSGDKDHRFEYSHGPITVLTNSNDPSTGPESTHPLPAKMHNYNYG
                     GNLQENPGGPSLMHGQTWTSPAQGPGYSQGYRGHISTSAGRGRGRGLPY"
     misc_feature    4375..6000
                     /note="Genewiz 5' Optimized"
                     /note="color: #0080ff"
     misc_feature    5906..6000
                     /note="SJD108_KpnI-strKozakATG-FH-opt5'CDK13_F"
                     /note="color: #50ff50"
     CDS             complement(5926..5952)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             complement(5953..5976)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     misc_feature    5976..5991
                     /label=Kozak
                     /note="color: #a6acb3"
     CDS             complement(5977..5979)
                     /codon_start=1
                     /label=startCodon
                     /note="color: #339966"
                     /translation="M"
     protein_bind    complement(6015..6039)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     misc_feature    complement(6025..6039)
                     /label=attR1
                     /note="ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff00ff
                     vntifkey=21"
                     /note="color: #ff00ff; direction: LEFT"
     misc_feature    6050..6055
                     /label=SpeI
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     misc_feature    complement(6083..6520)
                     /label=TetO
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#00ff00
                     vntifkey=21"
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    complement(6521..6526)
                     /label=XhoI
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    complement(6527)
                     /label=LoxP_mutation
                     /note="lox-P site
                     ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff00ff
                     vntifkey=21"
                     /note="color: #ff00ff; direction: LEFT"
     misc_feature    complement(6529..6725)
                     /label=bpA
                     /note="bovine growth hormone polyA_site
                     ApEinfo_fwdcolor=#ffff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff00ff
                     vntifkey=21"
                     /note="color: #ff00ff; direction: LEFT"
     CDS             complement(6764..7567)
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /label=neo
                     /note="ApEinfo_fwdcolor=#0000ff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#0000ff
                     vntifkey=4"
                     /note="color: #0000ff"
                     /db_xref="GI:13492216"
                     /protein_id="AAK28133.1"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(7576..7901)
                     /label=pgk(1)
                     /note="PGK
                     ApEinfo_fwdcolor=#80ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=pgk
                     ApEinfo_revcolor=#999999
                     vntifkey=29"
                     /note="color: #999999; direction: LEFT"
     promoter        complement(7902..8071)
                     /label=pgk
                     /note="PGK
                     ApEinfo_fwdcolor=#80ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#999999
                     vntifkey=29"
                     /note="color: #999999; direction: LEFT"
     intron          complement(7902..8071)
                     /label=sequenced
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=15"
                     /note="color: #ffc0cb; direction: LEFT"
     promoter        complement(8072..8352)
                     /label=SV40 promoter /origin(1) promoter
                     /label=SV40\promoter/origin(1)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=SV40\promoter/origin
                     ApEinfo_revcolor=pink
                     vntifkey=30"
                     /note="color: #ffc0cb; direction: LEFT"
     promoter        complement(8353..8391)
                     /label=SV40 promoter /origin promoter
                     /label=SV40\promoter/origin
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=30"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_binding    8392..8411
                     /label=HIV flap/nuclear import signal
                     /label=HIV\flap/nuclear\import\signal
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=20"
                     /note="color: #ffc0cb"
     misc_feature    join(8414,8419..8423)
                     /label=TetO(1)
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=TetO
                     ApEinfo_revcolor=#00ff00
                     vntifkey=21"
                     /note="This feature has 2 segments:
                        1: 8414 .. 8414 / #00ff00
                        2: 8419 .. 8423 / #00ff00"
     misc_feature    8433..8437
                     /label=ClaI
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     misc_feature    join(8446..8451,8462..8510)
                     /label=UpdatedBarcodeFragment
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="This feature has 2 segments:
                        1: 8446 .. 8451 / #00ffff
                        2: 8462 .. 8510 / #00ffff"
     misc_feature    join(8446..8451,8462..8470)
                     /label=HW146
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="This feature has 2 segments:
                        1: 8446 .. 8451 / #00ffff
                        2: 8462 .. 8470 / #00ffff"
     misc_feature    complement(8475..8500)
                     /label=HW147
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: LEFT"
     LTR             8514..8819
                     /label=pBac 5'- LTR
                     /label=pBac\5'-LTR
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=pink
                     vntifkey=19"
                     /note="color: #ffc0cb"
     misc_feature    complement(9312..9328)
                     /label=M13R
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    9653..11552
                     /label=CEN-URA3
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ff00"
     misc_feature    11591..12272
                     /label=colEI ori
                     /note="ApEinfo_fwdcolor=#c0c0c0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #c0c0c0"
     misc_feature    12369..13226
                     /label=Amp
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg
      661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
      721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa
      781 agataatcat gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
      841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
      901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct
      961 tctataaagt aacaaaactt ttatcgaatt cctgcagccc agcttgatcc cgcctcagaa
     1021 gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
     1081 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     1141 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     1201 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
     1261 cgggtttaaa cgggccctct agaggttagc gctagctcat tactacgtag aatcgagacc
     1321 gaggagaggg ttagggatag gcttaccaac cactttgtac aagaaagctg ggtcgaattc
     1381 gcccttctcg agtcagtatg gtaaccctct gcctcgacct ctgccagctg atgtgctaat
     1441 gtgtcccctg tatccttgtg aatatccagg tccttgggca ggagaagtcc aggtctgtcc
     1501 atgcatgagg ctagggccac ctggattttc ctgtaagtta ccaccatagt tatagttgtg
     1561 catctttgct ggcaagggat gagtactctc tggccctgtg gaagggtcat tgctgtttgt
     1621 gaggactgtg ataggaccat ggctatattc aaatctatgg tccttgtctc cactaaacaa
     1681 catgggacca gcattgaggt aaatgtctcc atatccagct actgaactgg caaacgactc
     1741 agtaaaggca gaagtttgag gtggaccacc agtgtgagat gaagcagtac tgtagttatc
     1801 cagagactga atatctgagt ttccaatgaa actacgtgct tccaatggtg cagcagcgga
     1861 gctacttcct agaccatcac tgctaacgta aggagcggca gagaaagaag atccaaagtt
     1921 gtccttatag tctgaactgg gcacatatgt gtctcctgtg ggataatcga taccaccttc
     1981 tcttttggga tcatcctggg gctgatgctg agcaagcagc tgtaagaggg ctgccttcac
     2041 tccagcatgt gggtcagtta atgaagaact agtggtagga acatgctggt tttctgtccg
     2101 ataatctagg tcctcctcag taactggtgg tggttcagga ggttcaggag gccgttgatc
     2161 aggaggcaga attcgaggcc ggtctggttc tggtgtcagc tccaatatcc tcctgtcttg
     2221 gtgctggatg aggtcatctt gcccggatcc aggagtgctc ggttctagag gaggcctgag
     2281 ttgcaatgga gcttcatgtc ctgatccatt ttccctttcc tctagcatgg catctttctg
     2341 tttggactgt tgggccttta ttaactgagt caagaggact gcaaatgcac tctgcacagc
     2401 agcctgggca gcatcagttt ccactttagg ctggatggtc tgtgaaggct gaactcctcc
     2461 caatgatttt gaagactcct gttgtggtgt tgatggatct gtctgttttt cacctgttgc
     2521 caaaattcca gcaggaaggt ttattttatt tagctgctgt tgagtctcag agtttacctt
     2581 aatgttcaac acttggacaa aatcagccat attaacactt gatttagatt gtagtaggtt
     2641 tagaagaatt gccaattcac tgtggtttaa cggctgtcca gggcctgtta ttacaggtgc
     2701 tacatttgag ttgctctgag atttcaactg tgcgggtggc agcacaccct ggggtgtgtt
     2761 agttctgctg tcatctaagc ccagagacag gtccttccta ggggctttga ttgtggaaag
     2821 atcatcagtc atgcccatct gtttctgtct tcttctcttt ttactccata attcatgaca
     2881 atcttgccac aaaggaaggt ctggtggagg cattttggag ggttccacgt ctcgcaggaa
     2941 ctcacactga agagcctgct cagcagtgca gcgcttactg ggatccaagg caagcatgta
     3001 atcgaataag tcgagtgcag ctgcggggat gaaaacaaat tcttctctta acttccgccg
     3061 atattgcttc tttggtttca tggtgttgaa atatggcagt ttgattacat caggccacac
     3121 tgcaggacat ggactcccac atatacggct tattagctct agctgtgcaa gttcctggtt
     3181 tgcttgaaat ataggttttt tagtgaagag ttcaccaagg atacatccac agctccatac
     3241 atcaatggct ggtgtatatc gttcttctcc caagagcaat tcaggtggac gataccacaa
     3301 agtaatgacc ttgttagtat atgggcgact ttcttctgag ctatataaac gagcaagtcc
     3361 aaaatctgca agttttatct gtcctctatt atttagaagg atatttgaac atttaatatc
     3421 tctatgcaaa aagttcttct tatgacaata atccaggcct tccatgagct gtctcataaa
     3481 agattttata tggttttcat taaaatgaac caagcctgat tccagcagtc ccatcagatc
     3541 atggtccata tattcaaaca ccaggtaaaa tgcacctttg tctttcttaa aatccaaagc
     3601 atcttcttta tcagtcacga tttccttcat attgatgata ctctggtggg tgagttgccg
     3661 aagaatttta atttctctaa ttgctgtaat tgggaaaccc tccttttcat tatccagacg
     3721 tactttcttt aaggctacca tttctcccgt gtctttgtcc ctggctttgt aaacttgtcc
     3781 ataagtacct tctccaataa ttccgatgat atcaaattta tccacgcagc gtttccccca
     3841 gtcaatatct ttttctttga tttcaccata gcgaggccca catattttag gccttcgttt
     3901 gctatgtaac tgtgctgctg ttttcttctc ctctggactc ttggaaagat catctcctcc
     3961 tggtaactca gggggcaatg gtaaatcagc aagcagacat cggagtttct tttctacttc
     4021 ttttttaact gctttgacag aaatgttgcc tcttaagcta tcagcatctt tatcttcagg
     4081 cagcatggga ggcaaaggta atggcggtaa cgtagaggtc actaaagcca catgctgctc
     4141 cttctccttg gctcctgtgc ttggtgtgag tggcttgctt ttctctttag tggaaactgg
     4201 ttcttccttt gtagcagcag gtttactctc cttcccaaca actgttttct tggtggcttt
     4261 ctctactgtc aaattattgt ctacctttgt tacttgaagc ggtggctttg tttttgcttt
     4321 gtcgcttttg acggtcccac cacttgaagg agaaggtgca tgctcagttt taagcttctt
     4381 gacttccttc acgtggttgg tctgggagac ggaggctccg gtctcggtgt ttcccttggt
     4441 gggtgtggaa gcgttggagg ccttagcagc cttggcggcg gcttcagcag ctttggcggc
     4501 ttcggcggct ttagcggctt cggcagctct agcagcctca gcggccctag ctttcttgtt
     4561 cttgttgagc tcggcggcca gggagctctt cagtgtcagg gtggaggggc tgatggagga
     4621 gtgcctgctt ctggacctgc tcagcctgtg cctggacctg cttctgctgt gcctggagga
     4681 gtaagggctc ctggacctgg acttggcgga cctcctcagc acaggggaat agggggatct
     4741 gctcctcctc caggaggagg agctgtaggg ggagggggag acatctcctc ctctctcgta
     4801 ggaggaatgc ctgctgtagc tgggggatct cctcctgctg taggggctag gggacctggg
     4861 cagtcttctg gaataagggc tgcttccgcc tccagcgggg gaagggcttt tcctggatct
     4921 cagggactgg ctagccctgt ggctgacagg ggactcgtct ctgcctccga gagggctgag
     4981 ggacctctgc ctcctcctgt aggccttggg ttcggacttg tcttccctgt aggctttggg
     5041 gggctccttg taggcggaag ggggttcctt ggagctcttg gtcctgctcc tatgagcctt
     5101 gaggtccctg tccttcctgg agctgctgct ggaggatgtg gcggaggcgg actttcttct
     5161 tccgccggag gagctggagg agccggattt ggcagcctcg gctctttcct cgccgctgtg
     5221 tccgtgtctg gatctggcct tgctggcctc ggatccgctc cttgtgccat ctctcctcct
     5281 atgttccctg tgcctctctt tggacctgcc gctggagctc cttctgtccc ttctaggtct
     5341 tctctcgctg ccctcggctc ttctctgggt tccgctgctg gaagcggggg atcctccatt
     5401 tcctccggtt cctccagcag cggtggcagc tgtagcagcg ctagctcctc ccaggagcag
     5461 gccttgttcg ctttggctgg acacgtcctc gtactccacc aggggtgtca ctcctcctcc
     5521 gctggaagct cctccgcctc catcctgctg gggttgaggc agggagaaca ctctcctctt
     5581 ttcggcttcc tgtccggccc taggtcctct ccttctcttt tgcctgccgc cgggtctcct
     5641 ttttcctctg gccagcctct tgacctccag aggagggcca gggctaaaac aggagctgga
     5701 agcggcagca gcagcggcgg cagcggcagc ggctccagga gcagccagaa acaggagagg
     5761 agggggagga ggagggggtt ggaggagctg aggttgcagg agagggagca gcagaggagg
     5821 ctgctgaggg ctgagaaacc tcctcctctt cctcctctcc tccagctttt tctcggccca
     5881 gctgagtccg cctcctccgc cgagagctgt atcggagctg gaaggagcgt aatcgggcac
     5941 gtcataaggg tacttgtcat cgtcgtcctt gtagtccatg gtggcggcgg taccagtagt
     6001 aagggcgaat tcggagcctg cttttttgta caaacttgtt gatatctgca ctagtggatc
     6061 cgagctcggt acgaattcgg ggccgcggag gctggatcgg tcccggtgtc ttctatggag
     6121 gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac gagctctgct
     6181 tatataggcc tcccaccgta cacgcctact cgacccgggt accgagctcg actttcactt
     6241 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
     6301 gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta aactcgactt
     6361 tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact
     6421 gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact
     6481 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgagttgc ctgctattgt
     6541 cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc
     6601 tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac
     6661 cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag
     6721 gcacagtcga ggctgatcag cgagctctag agaattgatc ccctcagaag aactcgtcaa
     6781 gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga
     6841 agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt
     6901 cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat
     6961 tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt
     7021 cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt
     7081 cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc
     7141 gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca
     7201 ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct
     7261 gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca
     7321 cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca
     7381 gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg
     7441 acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga
     7501 atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatggccg
     7561 atcccatatt ggctgcaggt cgaaaggccc ggagatgagg aagaggagaa cagcgcggca
     7621 gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc
     7681 ccgcccctga gcccgcccct gagcccgccc ccggacccac cccttcccag cctctgagcc
     7741 cagaaagcga aggagcaaag ctgctattgg ccgctgcccc aaaggcctac ccgcttccat
     7801 tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac gtgctacttc catttgtcac
     7861 gtcctgcacg acgcgagctg cggggcgggg gggaacttcc tgactagggg aggagtagaa
     7921 ggtggcgcga aggggccacc aaagaacgga gccggttggc gcctaccggt ggatgtggaa
     7981 tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg
     8041 catgctccag actgccttgg gaaaagtact gcctaggcct ccaaaaaagc ctcctcacta
     8101 cttctggaat agctcagagg cagaggcggc ctcggcctct gcataaataa aaaaaattag
     8161 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     8221 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     8281 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     8341 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cacagcagag
     8401 atccagtttg gttagatctc gaggtcgacg gtatcgttgg ccggtactcg agaagcgtat
     8461 gagatcggaa gagcggttca gcaggaatgc cgagaccgcg ccaacgataa gctatctata
     8521 acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata acaatataat
     8581 tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca
     8641 cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg
     8701 agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa
     8761 agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt
     8821 taatctagct gcatcaggat catatcgtcg ggtctttttt caattgctca gtcatcgccc
     8881 aagctggcgc tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga
     8941 agcggcatgg aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg
     9001 cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt
     9061 cgttcaggcc acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt
     9121 cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg
     9181 tgtgcagatt aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc
     9241 tggctggatg ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgcgcttg
     9301 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9361 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9421 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9481 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9601 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gacctgggtc
     9661 cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa
     9721 ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg
     9781 taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc
     9841 aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg
     9901 tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt
     9961 ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt
    10021 ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca
    10081 aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac
    10141 gaggcgcgtg taagttacag gcaagcgatc cgtcctaaga aaccattatt atcatgacat
    10201 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
    10261 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
    10321 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
    10381 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccacgctttt caattcaatt
    10441 catcattttt tttttattct tttttttgat ttcggtttct ttgaaatttt tttgattcgg
    10501 taatctccga acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac
    10561 gcatatgtag tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa
    10621 caaaaacctg caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg
    10681 ctactcatcc tagtcctgtt gctgccaagc tatttaatat catgcacgaa aagcaaacaa
    10741 acttgtgtgc ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat
    10801 taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg
    10861 agggcacagt taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgaag
    10921 acagaaaatt tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca
    10981 gaatagcaga atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta
    11041 gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag
    11101 cagaattgtc atgcaagggc tccctatcta ctggagaata tactaagggt actgttgaca
    11161 ttgcgaagag cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa
    11221 gagatgaagg ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag
    11281 acgcattggg tcaacagtat agaaccgtgg atgatgtggt ctctacagga tctgacatta
    11341 ttattgttgg aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt
    11401 acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaaaaactg
    11461 tattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc
    11521 agttattacc ctgcggtgtg aaataccgca cagagcaaaa ggccagcaaa aggccaggaa
    11581 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    11641 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    11701 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    11761 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    11821 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    11881 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    11941 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    12001 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    12061 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    12121 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    12181 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    12241 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    12301 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    12361 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    12421 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    12481 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    12541 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    12601 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    12661 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    12721 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    12781 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    12841 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    12901 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    12961 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    13021 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    13081 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    13141 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    13201 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    13261 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    13321 aggggttccg cgcacatttc cccgaaaagt gccac
//
