LOCUS pENTR_L1-YFP-Turbo-NES- 4397 bp ds-DNA circular 04-MAY-2019 DEFINITION . ACCESSION urn.local...2c-afa3se3 VERSION urn.local...2c-afa3se3 FEATURES Location/Qualifiers misc_feature 4230..4246 /label="M13 (-40) forward primer" rep_origin 3037..3710 /label="pUC origin" misc_feature 736..759 /label="GS linker" misc_feature complement(1838..1937) /label="attL2" misc_feature complement(1957..1973) /label="T7 promoter" misc_feature 4282..4381 /label="attL1" misc_feature 1768..1778 /label="NES" misc_feature complement(1978..1994) /label="M13 reverse primer" misc_feature 1780..1803 /label="NES" terminator 3981..4008 /label="rrnB T2 transcription terminator" misc_feature 4250..4265 /label="M13 (-20) forward primer" misc_feature complement(1954..1973) /label="T7 primer" misc_feature 1804..1827 /label="GS linker" terminator 4140..4183 /label="rrnB T1 transcription terminator" misc_feature 763..804 /label="V5 tag" CDS 1..717 /label="YFP" CDS 2107..2916 /label="Kan(R)" CDS 811..1767 /label="TurboID" ORIGIN 1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 181 ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag 241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 601 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggct 721 aagggtgggc gcgccggtgg aggcggttca ggaggtggca tgggcaagcc catccccaac 781 cccctgctgg gcctggacag caccgctagc aaagacaata ctgtgcctct gaagctgatc 841 gctctcctgg ctaatggcga gttccatagt ggcgaacagc tgggagaaac cctgggcatg 901 tccagggccg ctatcaacaa gcacattcag actctgcgcg actggggcgt ggacgtgttc 961 accgtgcccg gaaagggcta ctctctgccc gagcctatcc cgctgctgaa cgctaaacag 1021 attctgggac agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga ctccaccaat 1081 cagtacctgc tggatcgaat cggcgagctg aagagtgggg atgcttgcat tgcagaatat 1141 cagcaggcag ggagaggaag cagagggagg aaatggttct ctccttttgg agctaacctg 1201 tacctgagta tgttttggcg cctgaagcgg ggaccagcag caatcggcct gggcccggtc 1261 atcggaattg tcatggcaga agcgctgcga aagctgggag cagacaaggt gcgagtcaaa 1321 tggcccaatg acctgtatct gcaggataga aagctggcag gcatcctggt ggagctggcc 1381 ggaataacag gcgatgctgc acagatcgtc attggcgccg ggattaacgt ggctatgagg 1441 cgcgtggagg aaagcgtggt caatcagggc tggatcacac tgcaggaagc agggattaac 1501 ctggacagga atactctggc cgctacgctg atccgagagc tgcgggcagc cctggaactg 1561 ttcgagcagg aaggcctggc tccatatctg ccacggtggg agaagctgga taacttcatc 1621 aatagacccg tgaagctgat cattggggac aaagagattt tcgggattag ccgggggatt 1681 gataaacagg gagccctgct gctggaacag gacggagtta tcaaaccctg gatgggcgga 1741 gaaatcagtc tgcggtctgc cgaaaagctg cagctgcctc ccctggagcg cctgaccctg 1801 gacggaggcg gtggaagcgg cggaggtggg cgcgccgacc cagctttctt gtacaaagtt 1861 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa 1921 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc 1981 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa 2041 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg 2101 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat 2161 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc 2221 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc 2281 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct 2341 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg 2401 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt 2461 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct 2521 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg 2581 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa 2641 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca 2701 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc 2761 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct 2821 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa 2881 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa 2941 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt 3001 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 3061 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 3121 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 3181 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac 3241 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 3301 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 3361 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 3421 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa 3481 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 3541 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 3601 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 3661 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 3721 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 3781 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 3841 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 3901 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag 3961 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat 4021 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt 4081 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga 4141 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc 4201 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg 4261 gccagtctta agctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg 4321 ttgcaacaaa ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagcaggc 4381 tccgcggccg ccccctt //