LOCUS       pENTR_L1-YFP-miniTurbo- 4211 bp ds-DNA     circular     04-MAY-2019
DEFINITION  .
ACCESSION   urn.local...2a-afa3se3                                             
VERSION     urn.local...2a-afa3se3                                             
FEATURES             Location/Qualifiers
     misc_feature    4064..4079
                     /label="M13 (-20) forward primer"
     misc_feature    complement(1771..1787)
                     /label="T7 promoter"
     CDS             1..717
                     /label="YFP"
     CDS             1921..2730
                     /label="Kan(R)"
     terminator      3795..3822
                     /label="rrnB T2 transcription terminator"
     misc_feature    1582..1592
                     /label="NES"
     misc_feature    1594..1617
                     /label="NES"
     misc_feature    4044..4060
                     /label="M13 (-40) forward primer"
     terminator      3954..3997
                     /label="rrnB T1 transcription terminator"
     misc_feature    complement(1652..1751)
                     /label="attL2"
     misc_feature    complement(1768..1787)
                     /label="T7 primer"
     misc_feature    763..804
                     /label="V5-tag"
     rep_origin      2851..3524
                     /label="pUC origin"
     misc_feature    736..759
                     /label="GS linker"
     CDS             811..1581
                     /label="miniTurboID"
     misc_feature    1618..1641
                     /label="GS linker"
     misc_feature    4096..4195
                     /label="attL1"
     misc_feature    complement(1792..1808)
                     /label="M13 reverse primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggct
      721 aagggtgggc gcgccggtgg aggcggttca ggaggtggca tgggcaagcc catccccaac
      781 cccctgctgg gcctggacag caccgctagc atcccgctgc tgaacgctaa acagattctg
      841 ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg tcgactccac caatcagtac
      901 ctgctggatc gaatcggcga gctgaagagt ggggatgctt gcattgcaga atatcagcag
      961 gcagggagag gaagcagagg gaggaaatgg ttctctcctt ttggagctaa cctgtacctg
     1021 agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg gcctgggccc ggtcatcgga
     1081 attgtcatgg cagaagcgct gcgaaagctg ggagcagaca aggtgcgagt caaatggccc
     1141 aatgacctgt atctgcagga tagaaagctg gcaggcatcc tggtggagct ggccggaata
     1201 acaggcgatg ctgcacagat cgtcattggc gccgggatta acgtggctat gaggcgcgtg
     1261 gaggaaagcg tggtcaatca gggctggatc acactgcagg aagcagggat taacctggac
     1321 aggaatactc tggccgctat gctgatccga gagctgcggg cagccctgga actgttcgag
     1381 caggaaggcc tggctccata tctgtcacgg tgggagaagc tggataactt catcaataga
     1441 cccgtgaagc tgatcattgg ggacaaagag attttcggga ttagccgggg gattgataaa
     1501 cagggagccc tgctgctgga acaggacgga gttatcaaac cctggatggg cggagaaatc
     1561 agtctgcggt ctgccgaaaa gctgcagctg cctcccctgg agcgcctgac cctggacgga
     1621 ggcggtggaa gcggcggagg tgggcgcgcc gacccagctt tcttgtacaa agttggcatt
     1681 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1741 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     1801 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     1861 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     1921 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     1981 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     2041 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     2101 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     2161 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     2221 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     2281 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     2341 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     2401 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     2461 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     2521 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     2581 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     2641 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     2701 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     2761 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     2821 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2881 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2941 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3001 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     3061 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3121 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3181 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3241 accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga
     3301 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3361 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3421 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3481 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3541 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3601 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3661 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     3721 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     3781 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     3841 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     3901 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     3961 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     4021 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     4081 cttaagctcg ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
     4141 caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggctccgcg
     4201 gccgccccct t
//