LOCUS       pENTR_L1-YFP-miniTurbo- 4175 bp ds-DNA     circular     04-MAY-2019
DEFINITION  .
ACCESSION   urn.local...29-afa3se3                                             
VERSION     urn.local...29-afa3se3                                             
FEATURES             Location/Qualifiers
     misc_feature    4060..4159
                     /label="attL1"
     misc_feature    complement(1756..1772)
                     /label="M13 reverse primer"
     misc_feature    complement(1735..1751)
                     /label="T7 promoter"
     misc_feature    763..804
                     /label="V5 tag"
     terminator      3759..3786
                     /label="rrnB T2 transcription terminator"
     misc_feature    4028..4043
                     /label="M13 (-20) forward primer"
     misc_feature    complement(1732..1751)
                     /label="T7 primer"
     misc_feature    736..759
                     /label="GS linker"
     misc_feature    4008..4024
                     /label="M13 (-40) forward primer"
     misc_feature    1582..1605
                     /label="GS linker"
     rep_origin      2815..3488
                     /label="pUC origin"
     misc_feature    complement(1616..1715)
                     /label="attL2"
     CDS             1..717
                     /label="YFP"
     CDS             811..1581
                     /label="miniTurboID"
     terminator      3918..3961
                     /label="rrnB T1 transcription terminator"
     CDS             1885..2694
                     /label="Kan(R)"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggct
      721 aagggtgggc gcgccggtgg aggcggttca ggaggtggca tgggcaagcc catccccaac
      781 cccctgctgg gcctggacag caccgctagc atcccgctgc tgaacgctaa acagattctg
      841 ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg tcgactccac caatcagtac
      901 ctgctggatc gaatcggcga gctgaagagt ggggatgctt gcattgcaga atatcagcag
      961 gcagggagag gaagcagagg gaggaaatgg ttctctcctt ttggagctaa cctgtacctg
     1021 agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg gcctgggccc ggtcatcgga
     1081 attgtcatgg cagaagcgct gcgaaagctg ggagcagaca aggtgcgagt caaatggccc
     1141 aatgacctgt atctgcagga tagaaagctg gcaggcatcc tggtggagct ggccggaata
     1201 acaggcgatg ctgcacagat cgtcattggc gccgggatta acgtggctat gaggcgcgtg
     1261 gaggaaagcg tggtcaatca gggctggatc acactgcagg aagcagggat taacctggac
     1321 aggaatactc tggccgctat gctgatccga gagctgcggg cagccctgga actgttcgag
     1381 caggaaggcc tggctccata tctgtcacgg tgggagaagc tggataactt catcaataga
     1441 cccgtgaagc tgatcattgg ggacaaagag attttcggga ttagccgggg gattgataaa
     1501 cagggagccc tgctgctgga acaggacgga gttatcaaac cctggatggg cggagaaatc
     1561 agtctgcggt ctgccgaaaa gggaggcggt ggaagcggcg gaggtgggcg cgccgaccca
     1621 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     1681 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     1741 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     1801 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     1861 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     1921 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     1981 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     2041 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     2101 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     2161 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     2221 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     2281 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     2341 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     2401 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     2461 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     2521 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2581 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2641 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     2701 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     2761 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     2821 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2881 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2941 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3001 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3061 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3121 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3181 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa
     3241 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3301 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3361 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3421 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3481 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3541 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3601 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3661 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     3721 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     3781 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     3841 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     3901 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     3961 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     4021 cgacgttgta aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga
     4081 ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact
     4141 ttgtacaaaa aagcaggctc cgcggccgcc ccctt
//