LOCUS       pDONR-P2R-P3_R2-Turbo-m 4438 bp ds-DNA     circular     04-MAY-2019
DEFINITION  .
ACCESSION   urn.local...21-afa3se2                                             
VERSION     urn.local...21-afa3se2                                             
FEATURES             Location/Qualifiers
     misc_feature    786..827
                     /label="V5 tag"
     misc_feature    complement(2682..2698)
                     /label="T7 promoter"
     misc_feature    517..533
                     /label="M13 (-40) forward primer"
     misc_feature    complement(2679..2698)
                     /label="T7 primer"
     misc_feature    complement(2547..2643)
                     /label="attL3"
     misc_feature    1791..1814
                     /label="GS linker"
     misc_feature    537..552
                     /label="M13 (-20) forward primer"
     misc_feature    2544..2546
                     /label="STOP"
     misc_feature    759..782
                     /label="GS linker"
     CDS             1827..2543
                     /label="mVenus"
     terminator      427..470
                     /label="rrnB T1 transcription terminator"
     CDS             2832..3641
                     /label="Kan(R)"
     CDS             834..1790
                     /label="TurboID"
     misc_feature    complement(2703..2719)
                     /label="M13 reverse primer"
     misc_feature    complement(622..747)
                     /label="attR2"
     terminator      268..295
                     /label="rrnB T2 transcription terminator"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtggctg ggcgcgccgg tggaggcggt tcaggaggtg
      781 gcatgggcaa gcccatcccc aaccccctgc tgggcctgga cagcaccgct agcaaagaca
      841 atactgtgcc tctgaagctg atcgctctcc tggctaatgg cgagttccat agtggcgaac
      901 agctgggaga aaccctgggc atgtccaggg ccgctatcaa caagcacatt cagactctgc
      961 gcgactgggg cgtggacgtg ttcaccgtgc ccggaaaggg ctactctctg cccgagccta
     1021 tcccgctgct gaacgctaaa cagattctgg gacagctgga cggcgggagc gtggcagtcc
     1081 tgcctgtggt cgactccacc aatcagtacc tgctggatcg aatcggcgag ctgaagagtg
     1141 gggatgcttg cattgcagaa tatcagcagg cagggagagg aagcagaggg aggaaatggt
     1201 tctctccttt tggagctaac ctgtacctga gtatgttttg gcgcctgaag cggggaccag
     1261 cagcaatcgg cctgggcccg gtcatcggaa ttgtcatggc agaagcgctg cgaaagctgg
     1321 gagcagacaa ggtgcgagtc aaatggccca atgacctgta tctgcaggat agaaagctgg
     1381 caggcatcct ggtggagctg gccggaataa caggcgatgc tgcacagatc gtcattggcg
     1441 ccgggattaa cgtggctatg aggcgcgtgg aggaaagcgt ggtcaatcag ggctggatca
     1501 cactgcagga agcagggatt aacctggaca ggaatactct ggccgctacg ctgatccgag
     1561 agctgcgggc agccctggaa ctgttcgagc aggaaggcct ggctccatat ctgccacggt
     1621 gggagaagct ggataacttc atcaatagac ccgtgaagct gatcattggg gacaaagaga
     1681 ttttcgggat tagccggggg attgataaac agggagccct gctgctggaa caggacggag
     1741 ttatcaaacc ctggatgggc ggagaaatca gtctgcggtc tgccgaaaag ggaggcggtg
     1801 gaagcggcgg aggtgggcgc gcctccatgg tgagcaaggg cgaggagctg ttcaccgggg
     1861 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1921 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc tgcaccaccg
     1981 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc ctgcagtgct
     2041 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     2101 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     2161 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     2221 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     2281 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     2341 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     2401 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     2461 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     2521 tcggcatgga cgagctgtac aagtgacaac tttattatac aaagttggca ttataaaaaa
     2581 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     2641 ttggagctcc atggtagcgt taacgcggcc gcgatatccc ctatagtgag tcgtattaca
     2701 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     2761 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     2821 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     2881 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     2941 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     3001 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     3061 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     3121 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     3181 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     3241 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     3301 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     3361 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     3421 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     3481 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     3541 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     3601 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     3661 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     3721 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3781 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3841 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3901 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     3961 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4021 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4081 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4141 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     4201 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4261 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4321 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4381 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//