LOCUS pDONR-P2R-P3_R2-miniTur 4252 bp ds-DNA circular 04-MAY-2019 DEFINITION . ACCESSION urn.local...1z-afa3se2 VERSION urn.local...1z-afa3se2 FEATURES Location/Qualifiers misc_feature 2358..2360 /label="STOP" misc_feature 537..552 /label="M13 (-20) forward primer" CDS 1641..2357 /label="mVenus" misc_feature complement(2361..2457) /label="attL3" terminator 427..470 /label="rrnB T1 transcription terminator" CDS 2646..3455 /label="Kan(R)" misc_feature complement(2517..2533) /label="M13 reverse primer" misc_feature 1605..1628 /label="GS linker" misc_feature complement(622..747) /label="attR2" misc_feature complement(2493..2512) /label="T7 primer" misc_feature 517..533 /label="M13 (-40) forward primer" misc_feature 759..782 /label="GS linker" misc_feature complement(2496..2512) /label="T7 promoter" terminator 268..295 /label="rrnB T2 transcription terminator" CDS 834..1604 /label="miniTurboID" misc_feature 786..827 /label="V5 tag" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac 601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg 661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt 721 ctcgttcaac tttcttgtac aaagtggctg ggcgcgccgg tggaggcggt tcaggaggtg 781 gcatgggcaa gcccatcccc aaccccctgc tgggcctgga cagcaccgct agcatcccgc 841 tgctgaacgc taaacagatt ctgggacagc tggacggcgg gagcgtggca gtcctgcctg 901 tggtcgactc caccaatcag tacctgctgg atcgaatcgg cgagctgaag agtggggatg 961 cttgcattgc agaatatcag caggcaggga gaggaagcag agggaggaaa tggttctctc 1021 cttttggagc taacctgtac ctgagtatgt tttggcgcct gaagcgggga ccagcagcaa 1081 tcggcctggg cccggtcatc ggaattgtca tggcagaagc gctgcgaaag ctgggagcag 1141 acaaggtgcg agtcaaatgg cccaatgacc tgtatctgca ggatagaaag ctggcaggca 1201 tcctggtgga gctggccgga ataacaggcg atgctgcaca gatcgtcatt ggcgccggga 1261 ttaacgtggc tatgaggcgc gtggaggaaa gcgtggtcaa tcagggctgg atcacactgc 1321 aggaagcagg gattaacctg gacaggaata ctctggccgc tatgctgatc cgagagctgc 1381 gggcagccct ggaactgttc gagcaggaag gcctggctcc atatctgtca cggtgggaga 1441 agctggataa cttcatcaat agacccgtga agctgatcat tggggacaaa gagattttcg 1501 ggattagccg ggggattgat aaacagggag ccctgctgct ggaacaggac ggagttatca 1561 aaccctggat gggcggagaa atcagtctgc ggtctgccga aaagggaggc ggtggaagcg 1621 gcggaggtgg gcgcgcctcc atggtgagca agggcgagga gctgttcacc ggggtggtgc 1681 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 1741 gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc 1801 tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc 1861 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 1921 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 1981 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 2041 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 2101 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg 2161 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 2221 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg 2281 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 2341 tggacgagct gtacaagtga caactttatt atacaaagtt ggcattataa aaaagcattg 2401 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttggag 2461 ctccatggta gcgttaacgc ggccgcgata tcccctatag tgagtcgtat tacatggtca 2521 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag 2581 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg 2641 gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg 2701 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct 2761 atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg 2821 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc 2881 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga 2941 tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg 3001 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt 3061 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg 3121 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag 3181 aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac 3241 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg 3301 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc 3361 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat 3421 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac 3481 actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta 3541 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 3601 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 3661 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 3721 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 3781 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 3841 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 3901 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 3961 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag 4021 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 4081 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 4141 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 4201 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tt //