LOCUS       pDONR-P2R-P3_R2-miniTur 4252 bp ds-DNA     circular     04-MAY-2019
DEFINITION  .
ACCESSION   urn.local...1z-afa3se2                                             
VERSION     urn.local...1z-afa3se2                                             
FEATURES             Location/Qualifiers
     misc_feature    2358..2360
                     /label="STOP"
     misc_feature    537..552
                     /label="M13 (-20) forward primer"
     CDS             1641..2357
                     /label="mVenus"
     misc_feature    complement(2361..2457)
                     /label="attL3"
     terminator      427..470
                     /label="rrnB T1 transcription terminator"
     CDS             2646..3455
                     /label="Kan(R)"
     misc_feature    complement(2517..2533)
                     /label="M13 reverse primer"
     misc_feature    1605..1628
                     /label="GS linker"
     misc_feature    complement(622..747)
                     /label="attR2"
     misc_feature    complement(2493..2512)
                     /label="T7 primer"
     misc_feature    517..533
                     /label="M13 (-40) forward primer"
     misc_feature    759..782
                     /label="GS linker"
     misc_feature    complement(2496..2512)
                     /label="T7 promoter"
     terminator      268..295
                     /label="rrnB T2 transcription terminator"
     CDS             834..1604
                     /label="miniTurboID"
     misc_feature    786..827
                     /label="V5 tag"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtggctg ggcgcgccgg tggaggcggt tcaggaggtg
      781 gcatgggcaa gcccatcccc aaccccctgc tgggcctgga cagcaccgct agcatcccgc
      841 tgctgaacgc taaacagatt ctgggacagc tggacggcgg gagcgtggca gtcctgcctg
      901 tggtcgactc caccaatcag tacctgctgg atcgaatcgg cgagctgaag agtggggatg
      961 cttgcattgc agaatatcag caggcaggga gaggaagcag agggaggaaa tggttctctc
     1021 cttttggagc taacctgtac ctgagtatgt tttggcgcct gaagcgggga ccagcagcaa
     1081 tcggcctggg cccggtcatc ggaattgtca tggcagaagc gctgcgaaag ctgggagcag
     1141 acaaggtgcg agtcaaatgg cccaatgacc tgtatctgca ggatagaaag ctggcaggca
     1201 tcctggtgga gctggccgga ataacaggcg atgctgcaca gatcgtcatt ggcgccggga
     1261 ttaacgtggc tatgaggcgc gtggaggaaa gcgtggtcaa tcagggctgg atcacactgc
     1321 aggaagcagg gattaacctg gacaggaata ctctggccgc tatgctgatc cgagagctgc
     1381 gggcagccct ggaactgttc gagcaggaag gcctggctcc atatctgtca cggtgggaga
     1441 agctggataa cttcatcaat agacccgtga agctgatcat tggggacaaa gagattttcg
     1501 ggattagccg ggggattgat aaacagggag ccctgctgct ggaacaggac ggagttatca
     1561 aaccctggat gggcggagaa atcagtctgc ggtctgccga aaagggaggc ggtggaagcg
     1621 gcggaggtgg gcgcgcctcc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1681 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1741 gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc
     1801 tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc
     1861 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1921 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1981 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2041 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     2101 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
     2161 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2221 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
     2281 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2341 tggacgagct gtacaagtga caactttatt atacaaagtt ggcattataa aaaagcattg
     2401 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttggag
     2461 ctccatggta gcgttaacgc ggccgcgata tcccctatag tgagtcgtat tacatggtca
     2521 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     2581 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
     2641 gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg
     2701 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
     2761 atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
     2821 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     2881 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     2941 tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     3001 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     3061 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     3121 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     3181 aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     3241 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     3301 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     3361 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     3421 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
     3481 actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta
     3541 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3601 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3661 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3721 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     3781 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3841 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3901 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3961 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     4021 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4081 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4141 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4201 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//