LOCUS pENTR_L1-BirA(R118G)-NE 4367 bp ds-DNA circular 04-MAY-2019 DEFINITION . ACCESSION urn.local...23-afa3se2 VERSION urn.local...23-afa3se2 FEATURES Location/Qualifiers misc_feature 2470..2569 /label="attL1" CDS 3651..4367 /label="YFP" misc_feature 2583..2624 /label="V5 tag" rep_origin 1225..1898 /label="pUC origin" misc_feature complement(26..125) /label="attL2" misc_feature 3588..3598 /label="NES" misc_feature 1..3 /label="STOP" misc_feature 3624..3647 /label="GS linker" CDS 2631..3587 /label="humanized BirA(R118G)" misc_feature complement(145..161) /label="T7 promoter" misc_feature 2438..2453 /label="M13 (-20) forward primer" misc_feature 2418..2434 /label="M13 (-40) forward primer" misc_feature 3600..3623 /label="NES" terminator 2169..2196 /label="rrnB T2 transcription terminator" terminator 2328..2371 /label="rrnB T1 transcription terminator" misc_feature complement(166..182) /label="M13 reverse primer" CDS 295..1104 /label="Kan(R)" misc_feature complement(142..161) /label="T7 primer" ORIGIN 1 taagggccca agggtgggcg cgccgaccca gctttcttgt acaaagttgg cattataaga 61 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt 121 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc 181 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata 241 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc 301 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat 361 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat 421 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat 481 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc 541 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa 601 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg 661 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat 721 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt 781 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa 841 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt 901 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac 961 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag 1021 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat 1081 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc 1141 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac 1201 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 1261 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 1321 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 1381 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct 1441 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 1501 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 1561 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 1621 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg 1681 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 1741 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 1801 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 1861 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 1921 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 1981 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg 2041 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 2101 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta 2161 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt 2221 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc 2281 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg 2341 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg 2401 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag 2461 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt 2521 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc cgcggccgca 2581 tgggcaagcc catccccaac cccctgctgg gcctggacag caccgctagc aaggataaca 2641 ccgtgcccct gaaactgatt gctctgctgg ctaatggcga gttccatagt ggcgaacagc 2701 tgggagaaac cctgggcatg tccagggccg ctatcaacaa gcacattcag actctgcgcg 2761 actggggcgt ggacgtgttc accgtgcccg gaaagggcta ctctctgccc gagcctatcc 2821 agctgctgaa cgctaaacag attctgggac agctggacgg cgggagcgtg gcagtcctgc 2881 ctgtgatcga ctccaccaat cagtacctgc tggatcgaat cggcgagctg aagagtgggg 2941 atgcttgcat tgcagaatat cagcaggcag gaagaggagg gagagggagg aaatggttct 3001 ctccttttgg agctaacctg tacctgagta tgttttggcg cctggagcag ggaccagcag 3061 caatcggcct gagcctggtc atcggaattg tcatggcaga agtgctgcga aagctgggag 3121 cagacaaggt gcgagtcaaa tggcccaatg acctgtatct gcaggataga aagctggcag 3181 gcatcctggt ggagctgacc ggaaaaacag gcgatgctgc acagatcgtc attggcgccg 3241 ggattaacat ggctatgagg cgcgtggagg aaagcgtggt caatcagggc tggatcacac 3301 tgcaggaagc agggattaac ctggacagga atactctggc cgctatgctg atccgagagc 3361 tgcgggcagc cctggaactg ttcgagcagg aaggcctggc tccatatctg tcacggtggg 3421 agaagctgga taacttcatc aatagacccg tgaagctgat cattggggac aaagagattt 3481 tcgggattag ccggggaatt gataaacagg gagccctgct gctggaacag gacggaatta 3541 tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc cgaaaagctg cagctgcctc 3601 ccctggagcg cctgaccctg gacggaggcg gtggaagcgg cggaggttcc atggtgagca 3661 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 3721 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 3781 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 3841 ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact 3901 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 3961 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 4021 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 4081 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 4141 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 4201 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct 4261 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 4321 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaag //