LOCUS       pENTR_L1-Turbo-NES-YFP- 4367 bp ds-DNA     circular     04-MAY-2019
DEFINITION  .
ACCESSION   urn.local...27-afa3se3                                             
VERSION     urn.local...27-afa3se3                                             
FEATURES             Location/Qualifiers
     misc_feature    3624..3647
                     /label="GS linker"
     terminator      2169..2196
                     /label="rrnB T2 transcription terminator"
     misc_feature    complement(142..161)
                     /label="T7 primer"
     CDS             2631..3587
                     /label="TurboID"
     misc_feature    3588..3598
                     /label="NES"
     misc_feature    complement(145..161)
                     /label="T7 promoter"
     misc_feature    complement(26..125)
                     /label="attL2"
     misc_feature    complement(166..182)
                     /label="M13 reverse primer"
     misc_feature    3600..3623
                     /label="NES"
     misc_feature    2583..2624
                     /label="V5 tag"
     CDS             295..1104
                     /label="Kan(R)"
     misc_feature    2418..2434
                     /label="M13 (-40) forward primer"
     misc_feature    2438..2453
                     /label="M13 (-20) forward primer"
     CDS             3651..4367
                     /label="YFP"
     rep_origin      1225..1898
                     /label="pUC origin"
     misc_feature    1..3
                     /label="STOP"
     misc_feature    2470..2569
                     /label="attL1"
     terminator      2328..2371
                     /label="rrnB T1 transcription terminator"
ORIGIN
        1 taagggccca agggtgggcg cgccgaccca gctttcttgt acaaagttgg cattataaga
       61 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
      121 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
      181 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
      241 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
      301 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
      361 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
      421 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
      481 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
      541 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
      601 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
      661 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
      721 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
      781 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
      841 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
      901 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
      961 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     1021 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     1081 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     1141 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     1201 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1261 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1321 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1381 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     1441 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1501 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1561 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1621 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1681 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1741 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1801 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1861 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1921 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     1981 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     2041 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     2101 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     2161 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     2221 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     2281 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     2341 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     2401 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     2461 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
     2521 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc cgcggccgca
     2581 tgggcaagcc catccccaac cccctgctgg gcctggacag caccgctagc aaagacaata
     2641 ctgtgcctct gaagctgatc gctctcctgg ctaatggcga gttccatagt ggcgaacagc
     2701 tgggagaaac cctgggcatg tccagggccg ctatcaacaa gcacattcag actctgcgcg
     2761 actggggcgt ggacgtgttc accgtgcccg gaaagggcta ctctctgccc gagcctatcc
     2821 cgctgctgaa cgctaaacag attctgggac agctggacgg cgggagcgtg gcagtcctgc
     2881 ctgtggtcga ctccaccaat cagtacctgc tggatcgaat cggcgagctg aagagtgggg
     2941 atgcttgcat tgcagaatat cagcaggcag ggagaggaag cagagggagg aaatggttct
     3001 ctccttttgg agctaacctg tacctgagta tgttttggcg cctgaagcgg ggaccagcag
     3061 caatcggcct gggcccggtc atcggaattg tcatggcaga agcgctgcga aagctgggag
     3121 cagacaaggt gcgagtcaaa tggcccaatg acctgtatct gcaggataga aagctggcag
     3181 gcatcctggt ggagctggcc ggaataacag gcgatgctgc acagatcgtc attggcgccg
     3241 ggattaacgt ggctatgagg cgcgtggagg aaagcgtggt caatcagggc tggatcacac
     3301 tgcaggaagc agggattaac ctggacagga atactctggc cgctacgctg atccgagagc
     3361 tgcgggcagc cctggaactg ttcgagcagg aaggcctggc tccatatctg ccacggtggg
     3421 agaagctgga taacttcatc aatagacccg tgaagctgat cattggggac aaagagattt
     3481 tcgggattag ccgggggatt gataaacagg gagccctgct gctggaacag gacggagtta
     3541 tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc cgaaaagctg cagctgcctc
     3601 ccctggagcg cctgaccctg gacggaggcg gtggaagcgg cggaggttcc atggtgagca
     3661 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     3721 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     3781 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     3841 ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     3901 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     3961 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     4021 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     4081 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     4141 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     4201 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     4261 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     4321 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaag
//