LOCUS pENTR_L1-miniTurbo-NES- 4181 bp ds-DNA circular 04-MAY-2019 DEFINITION . ACCESSION urn.local...25-afa3se2 VERSION urn.local...25-afa3se2 FEATURES Location/Qualifiers misc_feature complement(142..161) /label="T7 primer" misc_feature 3414..3437 /label="NES" misc_feature 2438..2453 /label="M13 (-20) forward primer" rep_origin 1225..1898 /label="pUC origin" misc_feature 1..3 /label="STOP" misc_feature 2470..2569 /label="attL1" misc_feature 3402..3412 /label="NES" CDS 2631..3401 /label="miniTurboID" misc_feature complement(145..161) /label="T7 promoter" misc_feature 3438..3461 /label="GS linker" terminator 2169..2196 /label="rrnB T2 transcription terminator" terminator 2328..2371 /label="rrnB T1 transcription terminator" misc_feature 2418..2434 /label="M13 (-40) forward primer" misc_feature complement(166..182) /label="M13 reverse primer" misc_feature complement(26..125) /label="attL2" CDS 3465..4181 /label="YFP" misc_feature 2583..2624 /label="V5 tag" CDS 295..1104 /label="Kan(R)" ORIGIN 1 taagggccca agggtgggcg cgccgaccca gctttcttgt acaaagttgg cattataaga 61 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt 121 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc 181 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata 241 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc 301 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat 361 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat 421 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat 481 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc 541 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa 601 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg 661 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat 721 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt 781 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa 841 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt 901 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac 961 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag 1021 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat 1081 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc 1141 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac 1201 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 1261 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 1321 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 1381 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct 1441 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 1501 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 1561 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 1621 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg 1681 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 1741 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 1801 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 1861 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 1921 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 1981 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg 2041 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 2101 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta 2161 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt 2221 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc 2281 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg 2341 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg 2401 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag 2461 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt 2521 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc cgcggccgca 2581 tgggcaagcc catccccaac cccctgctgg gcctggacag caccgctagc atcccgctgc 2641 tgaacgctaa acagattctg ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg 2701 tcgactccac caatcagtac ctgctggatc gaatcggcga gctgaagagt ggggatgctt 2761 gcattgcaga atatcagcag gcagggagag gaagcagagg gaggaaatgg ttctctcctt 2821 ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg 2881 gcctgggccc ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg ggagcagaca 2941 aggtgcgagt caaatggccc aatgacctgt atctgcagga tagaaagctg gcaggcatcc 3001 tggtggagct ggccggaata acaggcgatg ctgcacagat cgtcattggc gccgggatta 3061 acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc acactgcagg 3121 aagcagggat taacctggac aggaatactc tggccgctat gctgatccga gagctgcggg 3181 cagccctgga actgttcgag caggaaggcc tggctccata tctgtcacgg tgggagaagc 3241 tggataactt catcaataga cccgtgaagc tgatcattgg ggacaaagag attttcggga 3301 ttagccgggg gattgataaa cagggagccc tgctgctgga acaggacgga gttatcaaac 3361 cctggatggg cggagaaatc agtctgcggt ctgccgaaaa gctgcagctg cctcccctgg 3421 agcgcctgac cctggacgga ggcggtggaa gcggcggagg ttccatggtg agcaagggcg 3481 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 3541 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 3601 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg 3661 gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca 3721 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 3781 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 3841 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 3901 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact 3961 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga 4021 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt 4081 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 4141 ccgccgccgg gatcactctc ggcatggacg agctgtacaa g //