LOCUS       pENTR_L1-miniTurbo-YFP- 4169 bp ds-DNA     circular     04-MAY-2019
DEFINITION  .
ACCESSION   urn.local...26-afa3se2                                             
VERSION     urn.local...26-afa3se2                                             
FEATURES             Location/Qualifiers
     misc_feature    2429..2444
                     /label="M13 (-20) forward primer"
     CDS             286..1095
                     /label="Kan(R)"
     terminator      2319..2362
                     /label="rrnB T1 transcription terminator"
     CDS             3420..4136
                     /label="YFP"
     misc_feature    3393..3416
                     /label="GS linker"
     misc_feature    2461..2560
                     /label="attL1"
     terminator      2160..2187
                     /label="rrnB T2 transcription terminator"
     misc_feature    4146..4166
                     /label="NLS"
     misc_feature    complement(136..152)
                     /label="T7 promoter"
     CDS             2622..3392
                     /label="miniTurboID"
     rep_origin      1216..1889
                     /label="pUC origin"
     misc_feature    complement(157..173)
                     /label="M13 reverse primer"
     misc_feature    complement(17..116)
                     /label="attL2"
     misc_feature    complement(133..152)
                     /label="T7 primer"
     misc_feature    2409..2425
                     /label="M13 (-40) forward primer"
     misc_feature    4167..4169
                     /label="STOP"
     misc_feature    2574..2615
                     /label="V5 tag"
ORIGIN
        1 aagggtgggc gcgccgaccc agctttcttg tacaaagttg gcattataag aaagcattgc
       61 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
      121 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
      181 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
      241 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
      301 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
      361 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
      421 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
      481 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
      541 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
      601 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
      661 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
      721 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
      781 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
      841 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
      901 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
      961 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     1021 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     1081 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     1141 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc atgggcaagc
     2581 ccatccccaa ccccctgctg ggcctggaca gcaccgctag catcccgctg ctgaacgcta
     2641 aacagattct gggacagctg gacggcggga gcgtggcagt cctgcctgtg gtcgactcca
     2701 ccaatcagta cctgctggat cgaatcggcg agctgaagag tggggatgct tgcattgcag
     2761 aatatcagca ggcagggaga ggaagcagag ggaggaaatg gttctctcct tttggagcta
     2821 acctgtacct gagtatgttt tggcgcctga agcggggacc agcagcaatc ggcctgggcc
     2881 cggtcatcgg aattgtcatg gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag
     2941 tcaaatggcc caatgacctg tatctgcagg atagaaagct ggcaggcatc ctggtggagc
     3001 tggccggaat aacaggcgat gctgcacaga tcgtcattgg cgccgggatt aacgtggcta
     3061 tgaggcgcgt ggaggaaagc gtggtcaatc agggctggat cacactgcag gaagcaggga
     3121 ttaacctgga caggaatact ctggccgcta tgctgatccg agagctgcgg gcagccctgg
     3181 aactgttcga gcaggaaggc ctggctccat atctgtcacg gtgggagaag ctggataact
     3241 tcatcaatag acccgtgaag ctgatcattg gggacaaaga gattttcggg attagccggg
     3301 ggattgataa acagggagcc ctgctgctgg aacaggacgg agttatcaaa ccctggatgg
     3361 gcggagaaat cagtctgcgg tctgccgaaa agggaggcgg tggaagcggc ggaggttcca
     3421 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     3481 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     3541 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     3601 tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
     3661 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     3721 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3781 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     3841 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     3901 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     3961 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     4021 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     4081 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggctt
     4141 cggatccaaa aaagaagaga aaggtatag
//