LOCUS       pBAD/His-miRFP670nano   4457 bp    DNA   circular            17-JAN-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|-12646002|
COMMENT     VNTDBDATE|-12646002|
COMMENT     VNTNAME|pBAD/His-miRFP670nano|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1218 " 1))))) "KpnI: 1 site") 
COMMENT                            5 1 "GGTACC" "CCATGG") 
COMMENT                      #116=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 66459312 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #117=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #118=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1659 " 1))))) "NheI: 1 site") 
COMMENT                            1 5 "GCTAGC" "CGATCG") 
COMMENT                      #119=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 66449608 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NotI: 0 sites") 2 6 "GCGGCCGC" 
COMMENT                            "CGCCGGCG") 
COMMENT                      #120=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 66471064 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "XbaI: 0 sites") 1 5 "TCTAGA" 
COMMENT                            "AGATCT"))) "Restriction/Methylation Map") 
COMMENT              #121=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 15 (12 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #122=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 0 (13 0 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #123=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 1 (14 1 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #124=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42535672 (4 #29# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #125=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #126=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1171 - 1659" 
COMMENT                                                    1))) "1171(d1) (163 codons)") 
COMMENT                                      #127=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42535616 (4 #41# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #128=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tag" 
COMMENT                                                    1) 
COMMENT                                              #129=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1780 - 1956" 
COMMENT                                                    1))) "1780(d1) (59 codons)") 
COMMENT                                      #130=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42534832 (4 #42# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #131=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #132=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4240 - 4407" 
COMMENT                                                    1))) "4240(d1) (56 codons)"))) 
COMMENT                                    "Phase #1 (3 frames)") 
COMMENT                              #133=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 2 (14 2 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #134=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42535728 (4 #27# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #135=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #136=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  512 - 757" 1))) 
COMMENT                                            "512(d2) (82 codons)") 
COMMENT                                      #137=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42535784 (4 #28# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #138=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #139=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  773 - 943" 1))) 
COMMENT                                            "773(d2) (57 codons)"))) 
COMMENT                                    "Phase #2 (2 frames)") 
COMMENT                              #140=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 3 (14 3 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #141=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42535280 (4 #30# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #142=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #143=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  84 - 260" 1))) 
COMMENT                                            "84(d3) (59 codons)") 
COMMENT                                      #144=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42535560 (4 #33# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #145=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #146=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2196 - 3053" 
COMMENT                                                    1))) "2196(d3) (286 codons)"))) 
COMMENT                                    "Phase #3 (2 frames)"))) "Direct strand") 
COMMENT                      #147=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 1 (13 1 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #148=(CFolderPar 
COMMENT                                    (CGroupPar (CParagraph 1 (0 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList)) "Phase #1 (0 frames)") 
COMMENT                              #149=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 2 (14 -2 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #150=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42535000 (4 #34# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #151=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #152=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1397 - 1567" 
COMMENT                                                    1))) "1397(c2) (57 codons)") 
COMMENT                                      #153=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42535336 (4 #35# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #154=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #155=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1724 - 2047" 
COMMENT                                                    1))) "1724(c2) (108 codons)") 
COMMENT                                      #156=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42535392 (4 #36# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #157=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #158=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2486 - 2653" 
COMMENT                                                    1))) "2486(c2) (56 codons)") 
COMMENT                                      #159=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42535448 (4 #37# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #160=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #161=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  2663 - 2926" 
COMMENT                                                    1))) "2663(c2) (88 codons)") 
COMMENT                                      #162=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42534888 (4 #38# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #163=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #164=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  3311 - 3562" 
COMMENT                                                    1))) "3311(c2) (84 codons)") 
COMMENT                                      #165=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42535504 (4 #39# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #166=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  tga" 
COMMENT                                                    1) 
COMMENT                                              #167=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  3872 - 4060" 
COMMENT                                                    1))) "3872(c2) (63 codons)") 
COMMENT                                      #168=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42535224 (4 #40# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #169=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: atg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #170=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  4408 - 877" 
COMMENT                                                    1))) "4408(c2) (309 codons)"))) 
COMMENT                                    "Phase #2 (7 frames)") 
COMMENT                              #171=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 3 (14 -3 0) 1 1 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #172=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42535056 (4 #31# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #173=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #174=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  370 - 621" 1))) 
COMMENT                                            "370(c3) (84 codons)") 
COMMENT                                      #175=(CFolderPar 
COMMENT                                            (CGroupPar 
COMMENT                                             (CParagraph 42534944 (4 #32# 0) 1 2 
COMMENT                                              3 0 192) 
COMMENT                                             (CObjectList 
COMMENT                                              #176=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) 
COMMENT                                                    "Start codon: gtg   Stop codon:  taa" 
COMMENT                                                    1) 
COMMENT                                              #177=(CLinePar 
COMMENT                                                    (CParagraph 0 (0 0) 1 2 4 0 
COMMENT                                                     180) "Region:  1168 - 1569" 
COMMENT                                                    1))) "1168(c3) (134 codons)"))) 
COMMENT                                    "Phase #3 (2 frames)"))) 
COMMENT                            "Complementary strand"))) "Open Reading Frames")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #178=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #179=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #180=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #181=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #182=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT                (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #183=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT                (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #184=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT                (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #185=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #186=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #187=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #188=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #189=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #190=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #191=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #192=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #193=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #194=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #195=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #196=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #197=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #198=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #199=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #200=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #201=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #202=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #203=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #204=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #205=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #206=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #207=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #208=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #209=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #210=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #211=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #212=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #213=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777113 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #214=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #215=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #216=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #217=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 
COMMENT         -1377) 1 1 0 1 1 
COMMENT        (mapper: 24.1079 -16.59 40.0378 40.0378 0.01 10 -1 4457 4457 1 0 0) 
COMMENT        #218=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 268632406 100) 
COMMENT              (CObjectList 
COMMENT               #219=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil 0 100) 
COMMENT                     (CObjectList 
COMMENT                      #220=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #184# 67693740 0) 
COMMENT                             (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 
COMMENT                             6.27559 6.27359 1 0.0214037) 0.140749) 
COMMENT                      #221=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #181# 0 100) 
COMMENT                            (LOGPEN 0 10 2147483648) 268633031 
COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 "pBAD/His-miRFP670nano" "@N" 1 0 
COMMENT                            0.871165 0 -10 14.1866 1.39868 Nil) 
COMMENT                      #222=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #180# 268633056 100) 
COMMENT                            (LOGPEN 1 400 1) 0 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "4457 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -10.8 2.59755 0.799245 Nil)) (CObjectList)) 
COMMENT               #223=(CGroupWidget (CWidget 13 (12 0) 1 2 0 0 Nil 1 100) 
COMMENT                     (CObjectList 
COMMENT                      #224=(CGroupWidget 
COMMENT                            (CWidget 0 (13 0 0) 1 2 0 0 Nil 1063777500 100) 
COMMENT                            (CObjectList 
COMMENT                             #225=(CGroupWidget 
COMMENT                                   (CWidget 1 (14 1 0) 1 2 0 0 Nil 268632461 100) 
COMMENT                                   (CObjectList 
COMMENT                                    #226=(CORFArrow 
COMMENT                                          (CLine 
COMMENT                                           (CWidget 1 (4 #29# 0) 1 2 0 0 #197# 
COMMENT                                            1411426528 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           1.16464 3.93816 4.62642) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 0 0 0) 
COMMENT                                    #227=(CORFArrow 
COMMENT                                          (CLine 
COMMENT                                           (CWidget 1 (4 #41# 0) 1 2 0 0 #197# 
COMMENT                                            64820448 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           1.16464 3.52013 3.76926) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 0 0 0) 
COMMENT                                    #228=(CORFArrow 
COMMENT                                          (CLine 
COMMENT                                           (CWidget 1 (4 #42# 0) 1 2 0 0 #197# 
COMMENT                                            64817088 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           1.16464 0.0703745 0.306833) 0.2 
COMMENT                                          0.609808 0.082322 3.41182 2.86186 0 0 
COMMENT                                          0)) (CObjectList)) 
COMMENT                             #229=(CGroupWidget 
COMMENT                                   (CWidget 2 (14 2 0) 1 2 0 0 Nil 1295816351 
COMMENT                                    100) 
COMMENT                                   (CObjectList 
COMMENT                                    #230=(CORFArrow 
COMMENT                                          (CLine 
COMMENT                                           (CWidget 2 (4 #27# 0) 1 2 0 0 #197# 
COMMENT                                            27778624 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           1.32929 5.20771 5.55396) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 0 0 0) 
COMMENT                                    #231=(CORFArrow 
COMMENT                                          (CLine 
COMMENT                                           (CWidget 2 (4 #28# 0) 1 2 0 0 #197# 
COMMENT                                            42756224 100) (LOGPEN 0 6 16384) 8 
COMMENT                                           1.32929 4.94592 5.1866) 0.2 0.609808 
COMMENT                                          0.082322 3.41182 2.86186 0 0 0)) 
COMMENT                                   (CObjectList)) 
COMMENT                             #232=(CGroupWidget 
COMMENT                                   (CWidget 3 (14 3 0) 1 2 0 0 Nil 1 100) 
COMMENT                                   (CObjectList 
COMMENT                                    #233=(CORFArrow 
COMMENT                                          (CLine 
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FEATURES             Location/Qualifiers
     misc_feature    1760..1917
                     /vntifkey="21"
                     /label=T1T2\rrnB\transcription\terminator
     misc_feature    2196..3056
                     /vntifkey="21"
                     /label=bla
     misc_feature    2097..2195
                     /vntifkey="21"
                     /label=bla\promoter
     misc_feature    3203..3875
                     /vntifkey="21"
                     /label=pBR322\origin
     misc_feature    complement(4405..826)
                     /vntifkey="21"
                     /label=araC
                     /note="araC"
     misc_feature    1219..1659
                     /vntifkey="21"
                     /label=miRFP670nano
     misc_feature    1180..1197
                     /vntifkey="21"
                     /label=6xHis
BASE COUNT     1115 a      1143 c      1148 g      1051 t 
ORIGIN
        1 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt 
       61 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct 
      121 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc 
      181 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat 
      241 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat 
      301 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa 
      361 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt 
      421 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat 
      481 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa 
      541 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg 
      601 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca 
      661 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga 
      721 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat 
      781 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc 
      841 cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct 
      901 tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac 
      961 aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa 
     1021 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca 
     1081 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc 
     1141 cgttttttgg gctaacagga ggaattaacc atggggagcc accatcacca tcaccatggc 
     1201 agatctgcag ctggtaccat ggcaaacctg gacaagatgc tgaataccac agtaacagag 
     1261 gtgcggcagt tcctgcaggt ggacagagtg tgcgtgttcc agtttgagga ggattatagc 
     1321 ggagtggtgg tggtggaggc cgtggacgat aggtggatct ccatcctgaa gacccaggtg 
     1381 cgggatagat acttcatgga gacaaggggc gaggagtatt ctcacggccg ctaccaggcc 
     1441 atcgccgaca tctacaccgc aaacctgaca gagtgctaca gggatctgct gacacagttt 
     1501 caggtgagag caatcctggc cgtgcccatc ctgcagggca agaagctgtg gggcctgttg 
     1561 gtggcacacc agctggcggc ccctagacag tggcagacct gggagatcga ctttctgaag 
     1621 cagcaggccg tggtggtggg catcgccatc cagcagagct agctgaattc gaagcttggc 
     1681 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc 
     1741 ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg 
     1801 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga 
     1861 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 
     1921 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga 
     1981 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc 
     2041 caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac 
     2101 tcttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 
     2161 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 
     2221 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 
     2281 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 
     2341 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 
     2401 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac 
     2461 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 
     2521 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 
     2581 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 
     2641 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 
     2701 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 
     2761 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 
     2821 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 
     2881 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 
     2941 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 
     3001 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 
     3061 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 
     3121 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 
     3181 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 
     3241 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 
     3301 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 
     3361 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 
     3421 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 
     3481 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 
     3541 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 
     3601 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 
     3661 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 
     3721 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 
     3781 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 
     3841 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 
     3901 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 
     3961 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc 
     4021 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg 
     4081 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc 
     4141 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 
     4201 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 
     4261 atcaccgaaa cgcgcgaggc agcagatcaa ttcgcgcgcg aaggcgaagc ggcatgcata 
     4321 atgtgcctgt caaatggacg aagcagggat tctgcaaacc ctatgctact ccgtcaagcc 
     4381 gtcaattgtc tgattcgtta ccaattatga caacttgacg gctacatcat tcactttttc 
     4441 ttcacaaccg gcacgga 
//
