LOCUS pTOX1_yhaV 5866 bp ds-DNA circular 08-MAY-2019 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT SphI GCATG/C COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 2656..3399 /locus_tag="rhaS-shuffled" /label="rhaS-shuffled" /ApEinfo_label="rhaS-shuffled" /ApEinfo_fwdcolor="#ff8040" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 4631..4717 /gene="Escherichia coli rrnB" /note="transcription terminator T1 from the E. coli rrnB gene" /locus_tag="rrnB T1 terminator" /label="rrnB T1 terminator" /ApEinfo_label="rrnB T1 terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 4809..4836 /note="transcription terminator T2 from the E. coli rrnB gene" /locus_tag="rrnB T2 terminator" /label="rrnB T2 terminator" /ApEinfo_label="rrnB T2 terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 5537..5783 /note="transcription terminator tL3 from phage lambda" /locus_tag="-lambda tL3 terminator" /label="-lambda tL3 terminator" /ApEinfo_label="-lambda tL3 terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 3768..3870 /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" /locus_tag="cat promoter" /label="cat promoter" /ApEinfo_label="cat promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 3871..4530 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF L KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFS S LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVAN M DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" /locus_tag="CmR" /label="CmR" /ApEinfo_label="CmR" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 3484..3517 /locus_tag="Strong forward terminator" /label="Strong forward terminator" /ApEinfo_label="Strong forward terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{} {} 0} width 5 offset 0" promoter 5039..5157 /note="promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)" /locus_tag="rhaB promoter" /label="rhaB promoter" /ApEinfo_label="rhaB promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5181..5477 /locus_tag="mqsR" /label="mqsR" /ApEinfo_label="mqsR" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 2454..2535 /gene="bla" /locus_tag="AmpR promoter" /label="AmpR promoter" /ApEinfo_label="AmpR promoter" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2563..2655 /locus_tag="rhaS-shuffled(1)" /label="rhaS-shuffled(1)" /ApEinfo_label="rhaS-shuffled" /ApEinfo_fwdcolor="#ff8040" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1..392 /locus_tag="R6K Ori" /label="R6K Ori" /ApEinfo_label="R6K Ori" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 2436..2453 /gene="bla" /locus_tag="AmpR promoter(1)" /label="AmpR promoter(1)" /ApEinfo_label="AmpR promoter" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 418..2417 /locus_tag="mob RP4" /label="mob RP4" /ApEinfo_label="mob RP4" /ApEinfo_fwdcolor="#00ff80" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 ccatgtcagc cgttaagtgt tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc 61 ttctcagtgc gttacatccc tggcttgttg tccacaaccg ttaaacctta aaagctttaa 121 aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct atttaagttg 181 ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa catgagagct 241 tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg 301 agagcttagt acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac 361 tatcaacagg ttgaactgct gatcttcaga tcctctacgc cggacgcatc gtggccggat 421 ccagccgacc aggctttcca cgcccgcgtg ccgctccatg tcgttcgcgc ggttctcgga 481 aacgcgctgc cgcgtttcgt gattgtcacg ctcaagcccg tagtcccgtt cgagcgtcgc 541 gcagaggtca gcgagggcgc ggtaggcccg atacggctca tggatggtgt ttcgggtcgg 601 gtgaatcttg ttgatggcga tatggatgtg caggttgtcg gtgtcgtgat gcacggcact 661 gacgcgctga tgctcggcga agccaagccc agcgcagatg cggtcctcaa tcgcgcgcaa 721 cgtctccgcg tcgggcttct ctcccgcgcg gaagctaacc agcaggtgat aggtcttgtc 781 ggcctcggaa cgggtgttgc cgtgctgggt cgccatcacc tcggccatga cagcgggcag 841 ggtgtttgcc tcgcagttcg tgacgcgcac gtgacccagg cgctcggtct tgccttgctc 901 gtcggtgatg tacttcacca gctccgcgaa gtcgctcttc ttgatggagc gcatggggac 961 gtgcttggca atcacgcgca ccccccggcc gttttagcgg ctaaaaaagt catggctctg 1021 ccctcgggcg gaccacgccc atcatgacct tgccaagctc gtcctgcttc tcttcgatct 1081 tcgccagcag ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg tcgtcggtga 1141 gccagagttt cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg gccagctcgc 1201 ggacgtgctc atagtccacg acgcccgtga ttttgtagcc ctggccgacg gccagcaggt 1261 aggccgacag gctcatgccg gccgccgccg ccttttcctc aatcgctctt cgttcgtctg 1321 gaaggcagta caccttgata ggtgggctgc ccttcctggt tggcttggtt tcatcagcca 1381 tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga gcaggattcc 1441 cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg 1501 ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac 1561 gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg 1621 ctataatgac cccgaagcag ggttatgcag cggaaaagcg ctgcttccct gctgttttgt 1681 ggaatatcta ccgactggaa acaggcaaat gcaggaaatt actgaactga ggggacaggc 1741 gagagacgat gccaaagagc tacaccgacg agctggccga gtgggttgaa tcccgcgcgg 1801 ccaagaagcg ccggcgtgat gaggctgcgg ttgcgttcct ggcggtgagg gcggatgtcg 1861 aggcggcgtt agcgtccggc tatgcgctcg tcaccatttg ggagcacatg cgggaaacgg 1921 ggaaggtcaa gttctcctac gagacgttcc gctcgcacgc caggcggcac atcaaggcca 1981 agcccgccga tgtgcccgca ccgcaggcca aggctgcgga acccgcgccg gcacccaaga 2041 cgccggagcc acggcggccg aagcaggggg gcaaggctga aaagccggcc cccgctgcgg 2101 ccccgaccgg cttcaccttc aacccaacac cggacaaaaa ggatcctcta cgccggacgc 2161 atcgtggccg gcatcaccgg cgccacaagt gcggttgctg gcgcctatat cgccgacatc 2221 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt 2281 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgct gcctcgcgcg 2341 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 2401 tctgtaagcg gatgccgtaa gtaaggtaaA GGCCTcgcgg aacccctatt tgtttatttt 2461 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 2521 aatattgaaa aaggaGCTTA TCACCGATAA GGAGGTTTTT TAATGACGGT GCTGCACTCG 2581 GTTGACTTCT TCCCTAGCGG CAATGCCAGC GTTGCCATTG AGCCGCGCCT GCCTCAAGCC 2641 GACTTCCCGG AGCACCACCA CGACTTCCAC GAGATCGTTA TCGTGGAGCA CGGTACCGGC 2701 ATCCACGTTT TCAACGGCCA ACCGTACACG ATTACGGGCG GTACCGTGTG CTTCGTTCGT 2761 GATCACGACC GCCACTTATA CGAGCACACG GACAACTTAT GCTTAACCAA CGTTTTATAC 2821 CGTAGCCCTG ACCGCTTCCA ATTCCTGGCG GGCTTAAACC AACTGTTACC GCAGGAATTA 2881 GACGGCCAAT ACCCTAGCCA TTGGCGTGTG AATCATTCGG TGCTGCAACA AGTTCGCCAA 2941 TTAGTGGCGC AAATGGAGCA ACAAGAGGGC GAGAACGACC TGCCGAGCAC GGCGAGCCGT 3001 GAAATTCTGT TCATGCAGCT GTTACTGCTG TTACGCAAGT CGAGCCTGCA AGAAAATTTA 3061 GAGAATTCGG CGAGCCGCCT GAATCTGCTG TTAGCGTGGT TAGAAGATCA CTTCGCGGAC 3121 GAAGTTAACT GGGATGCGGT TGCCGACCAG TTCAGCCTGA GCTTACGCAC CCTGCACCGC 3181 CAACTGAAAC AACAGACCGG CTTAACCCCG CAACGCTATT TAAACCGTTT ACGCTTAATG 3241 AAGGCGCGCC ACTTACTGCG CCATTCGGAA GCGTCGGTGA CCGATATTGC GTACCACTGT 3301 GGCTTTTCGG ATAGCAATCA TTTCAGCACC CTGTTCCGTC GCGAATTCAA TTGGAGCCCT 3361 CGCGACATCC GCCAAGGCCG CGACGGTTTC TTACAGTGAA GGCCTtctag agtcgacctg 3421 caggcatgca agcttggctg ttttggcgga tgagagGGTA CcgcatgAGC TTCGAGCTAA 3481 TCGcgcaaaa aaccccgctt cggcggggtt ttttcgcTGA TCACGTACGA TATCTTCGAA 3541 CCGGTGCACA TGGTGTACAG GGCCCTAGGA TAGGACGTCT TAAGGTTTAA ACCAGGTTAA 3601 TTAATTTAAA TGCATCCCGG GACGTCTCGA GCTCGATCGG ACCGCGGCCG CTAGCACGTA 3661 TACCAAGTGT CCTGTtttct tgccgccaag gatctgatgg cgcaggggat caaGATCTct 3721 gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta 3781 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta 3841 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc 3901 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa 3961 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa 4021 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat 4081 ccggaattac gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct 4141 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac 4201 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac 4261 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg 4321 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt 4381 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag 4441 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag 4501 tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg tcttcaaatt 4561 cccgttgcat cgatggcccc cgatggtagt gtggggtctc cccatgcgag agtagggaac 4621 tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 4681 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt 4741 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca 4801 aattaagcag aaggccatcc tgacggatgg cctttttgcg tggccagtgc caagcttgca 4861 tgcctgcagc tgtccggaac tagtcgaggc catggtggcc tcctgatgtc gtcaacacgg 4921 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa 4981 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca 5041 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc 5101 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg 5161 aaattcagca ggatcaccat atggaaaaac gcacaccaca tacacgtttg agtcaggtta 5221 aaaaacttgt caatgccggg caagttcgta caacacgtag tgccctgtta aatgcagatg 5281 agttaggttt ggattttgat ggtatgtgta atgttatcat tggattatca gagagcgact 5341 tttataaaag catgaccacc tactctgatc atactatctg gcaggatgtt tacagaccca 5401 ggcttgttac aggccaggtt tatcttaaaa ttacggtaat tcatgacgta ctgatcgtct 5461 cgtttaagga gaagtaagga tccgcgaatt ctcatgtttg acagcttatc actgatcagt 5521 gaattaatgg cgatgacgca tcctcacgat aatatccggg taggcgcaat cactttcgtc 5581 tctactccgt tacaaagcga ggctgggtat ttcccggcct ttctgttatc cgaaatccac 5641 tgaaagcaca gcggctggct gaggagataa ataataaacg aggggctgta tgcacaaagc 5701 atcttctgtt gagttaagaa cgagtatcga gatggcacat agccttgctc aaattggaat 5761 caggtttgtg ccaataccag tagaaacaga cgaagaagtc gtgggggaat tccacaaatt 5821 gttatccgct cacaattcca catgtggaat tccacatgtg gaattc //