LOCUS pTOX6_tse2_amilC 6837 bp ds-DNA circular 30-APR-2019 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT SphI GCATG/C COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 5297..6040 /locus_tag="rhaS-shuffled" /label="rhaS-shuffled" /ApEinfo_label="rhaS-shuffled" /ApEinfo_fwdcolor="#ff8040" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 5095..5176 /gene="bla" /locus_tag="AmpR promoter" /label="AmpR promoter" /ApEinfo_label="AmpR promoter" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5204..5296 /locus_tag="rhaS-shuffled(1)" /label="rhaS-shuffled(1)" /ApEinfo_label="rhaS-shuffled" /ApEinfo_fwdcolor="#ff8040" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2642..3033 /locus_tag="R6K Ori" /label="R6K Ori" /ApEinfo_label="R6K Ori" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 5077..5094 /gene="bla" /locus_tag="AmpR promoter(1)" /label="AmpR promoter(1)" /ApEinfo_label="AmpR promoter" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3059..5058 /locus_tag="mob RP4" /label="mob RP4" /ApEinfo_label="mob RP4" /ApEinfo_fwdcolor="#00ff80" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 1209..1295 /gene="Escherichia coli rrnB" /note="transcription terminator T1 from the E. coli rrnB gene" /locus_tag="rrnB T1 terminator" /label="rrnB T1 terminator" /ApEinfo_label="rrnB T1 terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 1387..1414 /note="transcription terminator T2 from the E. coli rrnB gene" /locus_tag="rrnB T2 terminator" /label="rrnB T2 terminator" /ApEinfo_label="rrnB T2 terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 2312..2558 /note="transcription terminator tL3 from phage lambda" /locus_tag="-lambda tL3 terminator" /label="-lambda tL3 terminator" /ApEinfo_label="-lambda tL3 terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 346..448 /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" /locus_tag="cat promoter" /label="cat promoter" /ApEinfo_label="cat promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 449..1108 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF L KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFS S LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVAN M DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" /locus_tag="CmR" /label="CmR" /ApEinfo_label="CmR" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 1706..1735 /note="promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)" /locus_tag="rhaB promoter" /label="rhaB promoter" /ApEinfo_label="rhaB promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 62..95 /locus_tag="Strong forward terminator" /label="Strong forward terminator" /ApEinfo_label="Strong forward terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{} {} 0} width 5 offset 0" promoter 1617..1705 /note="promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)" /locus_tag="rhaB promoter(1)" /label="rhaB promoter(1)" /ApEinfo_label="rhaB promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1776..2252 /locus_tag="tse2 toxin" /label="tse2 toxin" /ApEinfo_label="tse2 toxin" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 6075..6103 /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" /locus_tag="tac promoter" /label="tac promoter" /ApEinfo_label="tac promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 6170..6835 /locus_tag="amilCP" /label="amilCP" /ApEinfo_label="amilCP" /ApEinfo_fwdcolor="#0080ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 ggcatgcaag cttggctgtt ttggcggatg agagGGTACc gcatgAGCTT CGAGCTAATC 61 Gcgcaaaaaa ccccgcttcg gcggggtttt ttcgcTGATC ACGTACGATA TCTTCGAACC 121 GGTGCACATG GTGTACAGGG CCCTAGGATA GGACGTCTTA AGGTTTAAAC CAGGTTAATT 181 AATTTAAATG CATCCCGGGA CGTCTCGAGC TCGATCGGAC CGCGGCCGCT AGCACGTATA 241 CCAAGTGTCC TGTtttcttg ccgccaagga tctgatggcg caggggatca aGATCTctgt 301 tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag 361 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc 421 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt 481 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg 541 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa 601 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc 661 ggaattacgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg 721 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga 781 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct 841 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt 901 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt 961 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt 1021 tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta 1081 ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattgtc ttcaaattcc 1141 cgttgcatcg atggcccccg atggtagtgt ggggtctccc catgcgagag tagggaactg 1201 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1261 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg 1321 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa 1381 ttaagcagaa ggccatcctg acggatggcc tttttgcgtg gccagtgcca agcttgcatg 1441 cctgcagctg tccggaacta gtcgaggcca tggtggcctc ctgatgtcgt caacacggcg 1501 aaatagtaat cacgaggtca ggttcttacc ttaaattttc gacggaaaac cacgtaaaaa 1561 acgtcgattt ttcaagatac agcgtgaatt ttcaggaaat gcggtgagca tcacatcacc 1621 acaattcagc aaattgtgaa catcatcacg ttcatctttc cctggttgcc aatggcccat 1681 tttcctgtca gtaacgagaa ggtcgcgaat tcaggcgctt tttagactgg tcgtaatgaa 1741 attcagcagg atcaccatat gaggaggtaa aatagatgtc ctacgactac gagaaaacca 1801 gcctcaccct ctaccgggcg gtattcaagg ccaactacga cggcgacgtc ggtcgctacc 1861 tgcatcccga caaggaactc gccgaggctg cggaagtcgc cccgctgctg catccgacct 1921 tcgacagccc caacacccct ggcgtccccg cccgcgcgcc ggacatcgtc gccggccgcg 1981 acggcctcta cgccccggac accggcggca cctcggtgtt cgaccgcgcc ggcgtgctgc 2041 gccgcgccga cggcgacttc gtgatacccg acggcaccga catcccgccg gaccttaagg 2101 tgaagcagga cagctacaac aagcgcctgc aagccaccca ctacaccatc atgccggcca 2161 agccgatgta ccgggaggtc ctcatgggcc aactggacaa cttcgtgcgc aacgccatcc 2221 gccgccaatg ggaaaaagcc cgcgggctct agggatccgc gaattctcat gtttgacagc 2281 ttatcactga tcagtgaatt aatggcgatg acgcatcctc acgataatat ccgggtaggc 2341 gcaatcactt tcgtctctac tccgttacaa agcgaggctg ggtatttccc ggcctttctg 2401 ttatccgaaa tccactgaaa gcacagcggc tggctgagga gataaataat aaacgagggg 2461 ctgtatgcac aaagcatctt ctgttgagtt aagaacgagt atcgagatgg cacatagcct 2521 tgctcaaatt ggaatcaggt ttgtgccaat accagtagaa acagacgaag aagtcgtggg 2581 ggaattccac aaattgttat ccgctcacaa ttccacatgt ggaattccac atgtggaatt 2641 cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt gctttgagag gctctaaggg 2701 cttctcagtg cgttacatcc ctggcttgtt gtccacaacc gttaaacctt aaaagcttta 2761 aaagccttat atattctttt ttttcttata aaacttaaaa ccttagaggc tatttaagtt 2821 gctgatttat attaatttta ttgttcaaac atgagagctt agtacgtgaa acatgagagc 2881 ttagtacgtt agccatgaga gcttagtacg ttagccatga gggtttagtt cgttaaacat 2941 gagagcttag tacgttaaac atgagagctt agtacgtgaa acatgagagc ttagtacgta 3001 ctatcaacag gttgaactgc tgatcttcag atcctctacg ccggacgcat cgtggccgga 3061 tccagccgac caggctttcc acgcccgcgt gccgctccat gtcgttcgcg cggttctcgg 3121 aaacgcgctg ccgcgtttcg tgattgtcac gctcaagccc gtagtcccgt tcgagcgtcg 3181 cgcagaggtc agcgagggcg cggtaggccc gatacggctc atggatggtg tttcgggtcg 3241 ggtgaatctt gttgatggcg atatggatgt gcaggttgtc ggtgtcgtga tgcacggcac 3301 tgacgcgctg atgctcggcg aagccaagcc cagcgcagat gcggtcctca atcgcgcgca 3361 acgtctccgc gtcgggcttc tctcccgcgc ggaagctaac cagcaggtga taggtcttgt 3421 cggcctcgga acgggtgttg ccgtgctggg tcgccatcac ctcggccatg acagcgggca 3481 gggtgtttgc ctcgcagttc gtgacgcgca cgtgacccag gcgctcggtc ttgccttgct 3541 cgtcggtgat gtacttcacc agctccgcga agtcgctctt cttgatggag cgcatgggga 3601 cgtgcttggc aatcacgcgc accccccggc cgttttagcg gctaaaaaag tcatggctct 3661 gccctcgggc ggaccacgcc catcatgacc ttgccaagct cgtcctgctt ctcttcgatc 3721 ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg 3781 agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg 3841 cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac ggccagcagg 3901 taggccgaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct tcgttcgtct 3961 ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt ttcatcagcc 4021 atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc 4081 ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt 4141 gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca 4201 cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc 4261 gctataatga ccccgaagca gggttatgca gcggaaaagc gctgcttccc tgctgttttg 4321 tggaatatct accgactgga aacaggcaaa tgcaggaaat tactgaactg aggggacagg 4381 cgagagacga tgccaaagag ctacaccgac gagctggccg agtgggttga atcccgcgcg 4441 gccaagaagc gccggcgtga tgaggctgcg gttgcgttcc tggcggtgag ggcggatgtc 4501 gaggcggcgt tagcgtccgg ctatgcgctc gtcaccattt gggagcacat gcgggaaacg 4561 gggaaggtca agttctccta cgagacgttc cgctcgcacg ccaggcggca catcaaggcc 4621 aagcccgccg atgtgcccgc accgcaggcc aaggctgcgg aacccgcgcc ggcacccaag 4681 acgccggagc cacggcggcc gaagcagggg ggcaaggctg aaaagccggc ccccgctgcg 4741 gccccgaccg gcttcacctt caacccaaca ccggacaaaa aggatcctct acgccggacg 4801 catcgtggcc ggcatcaccg gcgccacaag tgcggttgct ggcgcctata tcgccgacat 4861 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg 4921 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc tgcctcgcgc 4981 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 5041 gtctgtaagc ggatgccgta agtaaggtaa AGGCCTcgcg gaacccctat ttgtttattt 5101 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 5161 taatattgaa aaaggaGCTT ATCACCGATA AGGAGGTTTT TTAATGACGG TGCTGCACTC 5221 GGTTGACTTC TTCCCTAGCG GCAATGCCAG CGTTGCCATT GAGCCGCGCC TGCCTCAAGC 5281 CGACTTCCCG GAGCACCACC ACGACTTCCA CGAGATCGTT ATCGTGGAGC ACGGTACCGG 5341 CATCCACGTT TTCAACGGCC AACCGTACAC GATTACGGGC GGTACCGTGT GCTTCGTTCG 5401 TGATCACGAC CGCCACTTAT ACGAGCACAC GGACAACTTA TGCTTAACCA ACGTTTTATA 5461 CCGTAGCCCT GACCGCTTCC AATTCCTGGC GGGCTTAAAC CAACTGTTAC CGCAGGAATT 5521 AGACGGCCAA TACCCTAGCC ATTGGCGTGT GAATCATTCG GTGCTGCAAC AAGTTCGCCA 5581 ATTAGTGGCG CAAATGGAGC AACAAGAGGG CGAGAACGAC CTGCCGAGCA CGGCGAGCCG 5641 TGAAATTCTG TTCATGCAGC TGTTACTGCT GTTACGCAAG TCGAGCCTGC AAGAAAATTT 5701 AGAGAATTCG GCGAGCCGCC TGAATCTGCT GTTAGCGTGG TTAGAAGATC ACTTCGCGGA 5761 CGAAGTTAAC TGGGATGCGG TTGCCGACCA GTTCAGCCTG AGCTTACGCA CCCTGCACCG 5821 CCAACTGAAA CAACAGACCG GCTTAACCCC GCAACGCTAT TTAAACCGTT TACGCTTAAT 5881 GAAGGCGCGC CACTTACTGC GCCATTCGGA AGCGTCGGTG ACCGATATTG CGTACCACTG 5941 TGGCTTTTCG GATAGCAATC ATTTCAGCAC CCTGTTCCGT CGCGAATTCA ATTGGAGCCC 6001 TCGCGACATC CGCCAAGGCC GCGACGGTTT CTTACAGTGA AGGCCTtcta gagtcgacct 6061 gcatcgacct gcagttgaca attaatcatc ggctcgtata atgtgtggaa aggcggttca 6121 ccgccgtttt cacacaggaa acagaattct ttaagaagga gatatacatA TGTCAGTGAT 6181 AGCAAAGCAG ATGACATACA AAGTATATAT GAGCGGTACT GTAAATGGTC ACTACTTCGA 6241 AGTAGAAGGT GATGGCAAAG GGAAGCCGTA TGAGGGTGAA CAAACAGTGA AGCTTACAGT 6301 TACGAAGGGT GGCCCTTTGC CGTTCGCGTG GGACATTTTG TCGCCACAGT GCCAGTACGG 6361 GAGCATACCA TTCACCAAGT ATCCAGAGGA CATACCAGAC TACGTGAAAC AGTCCTTTCC 6421 CGAAGGCTAT ACCTGGGAGC GCATAATGAA CTTTGAAGAC GGTGCGGTTT GTACGGTATC 6481 GAACGATTCA TCAATCCAAG GAAATTGCTT TATTTATCAT GTTAAATTTT CGGGGCTTAA 6541 CTTTCCGCCA AATGGCCCCG TGATGCAGAA GAAAACTCAG GGCTGGGAAC CAAATACCGA 6601 GCGCCTTTTC GCTCGGGACG GAATGCTGTT GGGAAACAAT TTTATGGCGT TGAAGCTGGA 6661 AGGGGGTGGC CACTATCTCT GTGAATTCAA GACAACATAC AAAGCCAAGA AGCCTGTCAA 6721 GATGCCCGGT TATCACTATG TGGACCGGAA GCTCGATGTC ACTAATCACA ACAAGGATTA 6781 TACTTCAGTT GAACAGTGCG AAATTTCGAT CGCTCGCAAA CCTGTCGTAG CATAAgg //