LOCUS pTOX10_yhaV 5681 bp ds-DNA circular 08-MAY-2019 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT SphI GCATG/C COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 4642..5385 /locus_tag="rhaS-shuffled" /label="rhaS-shuffled" /ApEinfo_label="rhaS-shuffled" /ApEinfo_fwdcolor="#ff8040" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 5470..5503 /locus_tag="Strong forward terminator" /label="Strong forward terminator" /ApEinfo_label="Strong forward terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{} {} 0} width 5 offset 0" promoter 4440..4521 /gene="bla" /locus_tag="AmpR promoter" /label="AmpR promoter" /ApEinfo_label="AmpR promoter" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4549..4641 /locus_tag="rhaS-shuffled(1)" /label="rhaS-shuffled(1)" /ApEinfo_label="rhaS-shuffled" /ApEinfo_fwdcolor="#ff8040" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1987..2378 /locus_tag="R6K Ori" /label="R6K Ori" /ApEinfo_label="R6K Ori" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 4422..4439 /gene="bla" /locus_tag="AmpR promoter(1)" /label="AmpR promoter(1)" /ApEinfo_label="AmpR promoter" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2404..4403 /locus_tag="mob RP4" /label="mob RP4" /ApEinfo_label="mob RP4" /ApEinfo_fwdcolor="#00ff80" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 751..837 /gene="Escherichia coli rrnB" /note="transcription terminator T1 from the E. coli rrnB gene" /locus_tag="rrnB T1 terminator" /label="rrnB T1 terminator" /ApEinfo_label="rrnB T1 terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 1159..1277 /note="promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006)" /locus_tag="rhaB promoter" /label="rhaB promoter" /ApEinfo_label="rhaB promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 929..956 /note="transcription terminator T2 from the E. coli rrnB gene" /locus_tag="rrnB T2 terminator" /label="rrnB T2 terminator" /ApEinfo_label="rrnB T2 terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1301..1597 /locus_tag="mqsR" /label="mqsR" /ApEinfo_label="mqsR" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 1657..1903 /note="transcription terminator tL3 from phage lambda" /locus_tag="-lambda tL3 terminator" /label="-lambda tL3 terminator" /ApEinfo_label="-lambda tL3 terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1..534 /locus_tag="aac1 gent-R" /label="aac1 gent-R" /ApEinfo_label="aac1 gent-R" /ApEinfo_fwdcolor="#ff80c0" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 TTAGGTGGCG GTACTTGGGT CGATATCAAA GTGCATCACT TCTTCCCGTA TGCCCAACTT 61 TGTATAGAGA GCCACTGCGG GATCGTCACC GTAATCTGCT TGCACGTAGA TCACATAAGC 121 ACCAAGCGCG TTGGCCTCAT GCTTGAGGAG ATTGATGAGC GCGGTGGCAA TGCCCTGCCT 181 CCGGTGCTCG CCGGAGACTG CGAGATCATA GATATAGATC TCACTACGCG GCTGCTCAAA 241 CTTGGGCAGA ACGTAAGCCG CGAGAGCGCC AACAACCGCT TCTTGGTCGA AGGCAGCAAG 301 CGCGATGAAT GTCTTACTAC GGAGCAAGTT CCCGAGGTAA TCGGAGTCCG GCTGATGTTG 361 GGAGTAGGTG GCTACGTCTC CGAACTCACG ACCGAAAAGA TCAAGAGCAG CCCGCATGGA 421 TTTGACTTGG TCAGGGCCGA GCCTACATGT GCGAATGATG CCCATACTTG AGCCACCTAA 481 CTTTGTTTTA GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTGCGTA ACATATGTTT 541 TTCCTCCTTA TGTTAAGCTT ACTCAGTTAT TATATCATAA ATATCTGTGT CAAGAATAAA 601 CTCCCACATG GATTCGacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag 661 gcagttattg tcttcaaatt cccgttgcat cgatggcccc cgatggtagt gtggggtctc 721 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac 781 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg 841 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg 901 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg 961 tggccagtgc caagcttgca tgcctgcagc tgtccggaac tagtcgaggc catggtggcc 1021 tcctgatgtc gtcaacacgg cgaaatagta atcacgaggt caggttctta ccttaaattt 1081 tcgacggaaa accacgtaaa aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa 1141 atgcggtgag catcacatca ccacaattca gcaaattgtg aacatcatca cgttcatctt 1201 tccctggttg ccaatggccc attttcctgt cagtaacgag aaggtcgcga attcaggcgc 1261 tttttagact ggtcgtaatg aaattcagca ggatcaccat atggaaaaac gcacaccaca 1321 tacacgtttg agtcaggtta aaaaacttgt caatgccggg caagttcgta caacacgtag 1381 tgccctgtta aatgcagatg agttaggttt ggattttgat ggtatgtgta atgttatcat 1441 tggattatca gagagcgact tttataaaag catgaccacc tactctgatc atactatctg 1501 gcaggatgtt tacagaccca ggcttgttac aggccaggtt tatcttaaaa ttacggtaat 1561 tcatgacgta ctgatcgtct cgtttaagga gaagtaagga tccgcgaatt ctcatgtttg 1621 acagcttatc actgatcagt gaattaatgg cgatgacgca tcctcacgat aatatccggg 1681 taggcgcaat cactttcgtc tctactccgt tacaaagcga ggctgggtat ttcccggcct 1741 ttctgttatc cgaaatccac tgaaagcaca gcggctggct gaggagataa ataataaacg 1801 aggggctgta tgcacaaagc atcttctgtt gagttaagaa cgagtatcga gatggcacat 1861 agccttgctc aaattggaat caggtttgtg ccaataccag tagaaacaga cgaagaagtc 1921 gtgggggaat tccacaaatt gttatccgct cacaattcca catgtggaat tccacatgtg 1981 gaattcccat gtcagccgtt aagtgttcct gtgtcactca aaattgcttt gagaggctct 2041 aagggcttct cagtgcgtta catccctggc ttgttgtcca caaccgttaa accttaaaag 2101 ctttaaaagc cttatatatt cttttttttc ttataaaact taaaacctta gaggctattt 2161 aagttgctga tttatattaa ttttattgtt caaacatgag agcttagtac gtgaaacatg 2221 agagcttagt acgttagcca tgagagctta gtacgttagc catgagggtt tagttcgtta 2281 aacatgagag cttagtacgt taaacatgag agcttagtac gtgaaacatg agagcttagt 2341 acgtactatc aacaggttga actgctgatc ttcagatcct ctacgccgga cgcatcgtgg 2401 ccggatccag ccgaccaggc tttccacgcc cgcgtgccgc tccatgtcgt tcgcgcggtt 2461 ctcggaaacg cgctgccgcg tttcgtgatt gtcacgctca agcccgtagt cccgttcgag 2521 cgtcgcgcag aggtcagcga gggcgcggta ggcccgatac ggctcatgga tggtgtttcg 2581 ggtcgggtga atcttgttga tggcgatatg gatgtgcagg ttgtcggtgt cgtgatgcac 2641 ggcactgacg cgctgatgct cggcgaagcc aagcccagcg cagatgcggt cctcaatcgc 2701 gcgcaacgtc tccgcgtcgg gcttctctcc cgcgcggaag ctaaccagca ggtgataggt 2761 cttgtcggcc tcggaacggg tgttgccgtg ctgggtcgcc atcacctcgg ccatgacagc 2821 gggcagggtg tttgcctcgc agttcgtgac gcgcacgtga cccaggcgct cggtcttgcc 2881 ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg ctcttcttga tggagcgcat 2941 ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg 3001 gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc tgcttctctt 3061 cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt 3121 cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg atgcgggcca 3181 gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg ccgacggcca 3241 gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt 3301 cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat 3361 cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag 3421 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg 3481 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt 3541 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa 3601 aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagcgctgc ttccctgctg 3661 ttttgtggaa tatctaccga ctggaaacag gcaaatgcag gaaattactg aactgagggg 3721 acaggcgaga gacgatgcca aagagctaca ccgacgagct ggccgagtgg gttgaatccc 3781 gcgcggccaa gaagcgccgg cgtgatgagg ctgcggttgc gttcctggcg gtgagggcgg 3841 atgtcgaggc ggcgttagcg tccggctatg cgctcgtcac catttgggag cacatgcggg 3901 aaacggggaa ggtcaagttc tcctacgaga cgttccgctc gcacgccagg cggcacatca 3961 aggccaagcc cgccgatgtg cccgcaccgc aggccaaggc tgcggaaccc gcgccggcac 4021 ccaagacgcc ggagccacgg cggccgaagc aggggggcaa ggctgaaaag ccggcccccg 4081 ctgcggcccc gaccggcttc accttcaacc caacaccgga caaaaaggat cctctacgcc 4141 ggacgcatcg tggccggcat caccggcgcc acaagtgcgg ttgctggcgc ctatatcgcc 4201 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc 4261 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgctgcct 4321 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 4381 agcttgtctg taagcggatg ccgtaagtaa ggtaaAGGCC Tcgcggaacc cctatttgtt 4441 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 4501 ttcaataata ttgaaaaagg aGCTTATCAC CGATAAGGAG GTTTTTTAAT GACGGTGCTG 4561 CACTCGGTTG ACTTCTTCCC TAGCGGCAAT GCCAGCGTTG CCATTGAGCC GCGCCTGCCT 4621 CAAGCCGACT TCCCGGAGCA CCACCACGAC TTCCACGAGA TCGTTATCGT GGAGCACGGT 4681 ACCGGCATCC ACGTTTTCAA CGGCCAACCG TACACGATTA CGGGCGGTAC CGTGTGCTTC 4741 GTTCGTGATC ACGACCGCCA CTTATACGAG CACACGGACA ACTTATGCTT AACCAACGTT 4801 TTATACCGTA GCCCTGACCG CTTCCAATTC CTGGCGGGCT TAAACCAACT GTTACCGCAG 4861 GAATTAGACG GCCAATACCC TAGCCATTGG CGTGTGAATC ATTCGGTGCT GCAACAAGTT 4921 CGCCAATTAG TGGCGCAAAT GGAGCAACAA GAGGGCGAGA ACGACCTGCC GAGCACGGCG 4981 AGCCGTGAAA TTCTGTTCAT GCAGCTGTTA CTGCTGTTAC GCAAGTCGAG CCTGCAAGAA 5041 AATTTAGAGA ATTCGGCGAG CCGCCTGAAT CTGCTGTTAG CGTGGTTAGA AGATCACTTC 5101 GCGGACGAAG TTAACTGGGA TGCGGTTGCC GACCAGTTCA GCCTGAGCTT ACGCACCCTG 5161 CACCGCCAAC TGAAACAACA GACCGGCTTA ACCCCGCAAC GCTATTTAAA CCGTTTACGC 5221 TTAATGAAGG CGCGCCACTT ACTGCGCCAT TCGGAAGCGT CGGTGACCGA TATTGCGTAC 5281 CACTGTGGCT TTTCGGATAG CAATCATTTC AGCACCCTGT TCCGTCGCGA ATTCAATTGG 5341 AGCCCTCGCG ACATCCGCCA AGGCCGCGAC GGTTTCTTAC AGTGAAGGCC Ttctagagtc 5401 gacctgcagg catgcaagct tggctgtttt ggcggatgag agGGTACcgc atgAGCTTCG 5461 AGCTAATCGc gcaaaaaacc ccgcttcggc ggggtttttt cgcTGATCAC GTACGATATC 5521 TTCGAACCGG TGCACATGGT GTACAGGGCC CTAGGATAGG ACGTCTTAAG GTTTAAACCA 5581 GGTTAATTAA TTTAAATGCA TCCCGGGACG TCTCGAGCTC GATCGGACCG CGGCCGCTAG 5641 CACGTATACC AAGTGTCCTG Ttttcttgcc gccaaggatc t //