LOCUS       CROPseq-Puro-dang      10224 bp ds-DNA     circular     04-OCT-2018
DEFINITION  .
ACCESSION   urn.local...b-6pix0yf                                              
SOURCE      natural DNA sequence                                               
  ORGANISM  unspecified                                                        
FEATURES             Location/Qualifiers
     CDS             3854..4660
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     LTR             10189..10224
                     /label="3' LTR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     LTR             2300..2499
                     /label="3' LTR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    7637..7638
                     /label="origin"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2266..2271
                     /label="Pol III stop"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             8915..9514
                     /label="Puro"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    6212..6349
                     /label="psi"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2197..2200
                     /label="loop 1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2184..2184
                     /label="Dang_2015_M"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     polyA_signal    2576..2706
                     /label="SV40 polyA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2215..2215
                     /label="Dang_2015_M"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_RNA        9530..10118
                     /label="WPRE"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        50..294
                     /label="hU6"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2173..2178
                     /label="BsmBI"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2192..2196
                     /label="Dang_2015_plus-5"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6826..7067
                     /label="RRE"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    7468..7585
                     /label="cPPT"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     LTR             5749..6161
                     /label="5' LTR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2180..2265
                     /label="scaffold_Dang_2015"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2201..2205
                     /label="Dang_2015_plus-5"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_binding    295..2179
                     /label="filler (1,885 bp)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        7644..8902
                     /label="EF-1a"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(300..305)
                     /label="BsmBI"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     rep_origin      complement(4786..5401)
                     /label="pUC Ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     9890..9909
                     /label="gRNA validation primer 5'-TTGGGCACTGACAATTCCGT-3'"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6316..6680
                     /label="gag"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccagag agggcctatt
       61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
      121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
      301 agacggttgt aaatgagcac acaaaataca catgctaaaa tattatattc tatgaccttt
      361 ataaaatcaa ccaaaatctt ctttttaata actttagtat caataattag aatttttatg
      421 ttcctttttg caaactttta ataaaaatga gcaaaataaa aaaacgctag ttttagtaac
      481 tcgcgttgtt ttcttcacct ttaataatag ctactccacc acttgttcct aagcggtcag
      541 ctcctgcttc aatcattttt tgagcatctt caaatgttct aactccacca gctgctttaa
      601 ctaaagcatt gtctttaaca actgacttca ttagtttaac atcttcaaat gttgcacctg
      661 attttgaaaa tcctgttgat gttttaacaa attctaatcc agcttcaaca gctatttcac
      721 aagctttcat gatttcttct tttgttaata aacaattttc cataatacat ttaacaacat
      781 gtgatccagc tgcttttttt acagctttca tgtcttctaa aactaattca taatttttgt
      841 cttttaatgc accaatattt aataccatat caatttctgt tgcaccatct ttaattgctt
      901 cagaaacttc gaatgctttt gtagctgttg tgcatgcacc tagaggaaaa cctacaacat
      961 ttgttattcc tacatttgtg ccttttaata attctttaca atagcttgtt caatatgaat
     1021 taacacaaac tgttgcaaaa tcaaattcaa ttgcttcatc acataattgt ttaatttcag
     1081 ctttcgtagc atcttgtttt aataatgtgt gatctatata tttgtttagt ttcatttttt
     1141 ctcctatata ttcattttta attttaattc tttaataatt tcgtctactt taactttagc
     1201 gttttgaaca gattcaccaa cacctataaa ataaattttt agtttaggtt cagttccact
     1261 tgggcgaaca gcaaatcatg acttatcttc taaataaaat tttagtaagt cttgtcctgg
     1321 catattatac attccatcga tgtagtcttc aacattaaca actttaagtc cagcaatttg
     1381 agttaagggt gttgctctca atgatttcat taatggttca atttttaatt tcttttcttc
     1441 tggtttaaaa ttcaagttta aagtgaaagt gtaatatgca cccatttctt taaataaatc
     1501 ttctaaatag tctactaatg ttttattttg ttttttataa aatcaagcag cctctgctat
     1561 taatatagaa gcttgtattc catctttatc tctagctgag tcatcaatta catatccata
     1621 actttcttca taagcaaaaa caaaatttaa tccgttatct tcttctttag caatttctct
     1681 acccattcat ttaaatccag ttaaagtttt tacaatatta actccatatt tttcatgagc
     1741 gattctatca cccaaatcac ttgttacaaa acttgaatat agagccggat tttttggaat
     1801 gctatttaag cgttttagat ttgataattt tcaatcaatt aaaattggtc ctgtttgatt
     1861 tccatctaat cttacaaaat gaccatcatg ttttattgcc attccaaatc tgtcagcatc
     1921 tgggtcattc ataataataa tatctgcatc atgtttaata ccatattcaa gcggtatttt
     1981 tcatgcagga tcaaattctg gatttggatt tacaacattt ttaaatgttt catcttcaaa
     2041 tgcatgctct tcaacctcaa taacgttata tcctgattca cgtaatattt ttggggtaaa
     2101 tttagttcct gttccattaa ctgcgctaaa aataattttt aaatcttttt tagcttcttg
     2161 ctcttttttg tacgtctctG TTTCAGAGCT ATGCTGGAAA CAGCATAGCA AGTTGAAATA
     2221 AGGCTAGTCC GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTgcttttt taagcttggc
     2281 gtaactagat cttgagacac tgctttttgc ttgtactggg tctctctggt tagaccagat
     2341 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     2401 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     2461 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca
     2521 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     2581 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2641 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     2701 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     2761 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     2821 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     2881 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     2941 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     3001 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     3061 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3121 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3181 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3241 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     3301 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     3361 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     3421 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     3481 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     3541 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3601 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3661 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3721 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3781 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3841 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3901 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3961 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4021 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4081 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4141 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4201 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4261 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4321 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4381 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4441 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4501 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4561 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4621 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4681 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4741 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4801 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4861 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4921 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4981 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5041 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5101 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5161 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5221 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5281 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5341 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5401 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5461 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     5521 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     5581 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     5641 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     5701 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     5761 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     5821 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     5881 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     5941 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     6001 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6061 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6121 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     6181 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     6241 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     6301 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     6361 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     6421 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     6481 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     6541 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     6601 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     6661 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     6721 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     6781 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     6841 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     6901 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     6961 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     7021 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     7081 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     7141 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     7201 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     7261 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     7321 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     7381 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagaatctt
     7441 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     7501 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     7561 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     7621 gcgccggctc gagggggccc gggtgcaaag atggataaag ttttaaacag agaggaatct
     7681 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     7741 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     7801 atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     7861 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     7921 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     7981 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     8041 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     8101 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     8161 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     8221 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     8281 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     8341 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     8401 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     8461 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     8521 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     8581 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     8641 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     8701 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     8761 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     8821 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     8881 tcttccattt caggtgtcgt gacgtacggc caccatgacc gagtacaagc ccacggtgcg
     8941 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     9001 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     9061 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     9121 cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     9181 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     9241 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     9301 agtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     9361 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     9421 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     9481 cacctggtgc atgacccgca agcccggtgc ctgaacgcgt taagtcgaca atcaacctct
     9541 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     9601 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     9661 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     9721 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     9781 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     9841 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     9901 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     9961 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
    10021 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
    10081 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact
    10141 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
    10201 attcactccc aacgaagaca agat
//