LOCUS       pES134_=_pKD13+F3-aadA- 3099 bp ds-DNA     circular     19-JUL-2018
DEFINITION  .
KEYWORDS    "accession:AY048744.1"                                             
FEATURES             Location/Qualifiers
     misc_feature    2016..2034
                     /label="priming site 4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(join(2827..3099,1..588))
                     /label="bla; ampicillin resistance (beta-lactamase)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2083..2325
                     /label="lambda terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(2110..2132)
                     /label="E193"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      2359..2757
                     /label="R6K gamma replication origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    735..994
                     /label="rrnB transcription terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    975..997
                     /label="E194"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(1101..1892)
                     /label="aadA (SpecR)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     RBS             complement(1893..1914)
                     /label="B0034m"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(1954..1987)
                     /label="F3 (FRT)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1996..2015)
                     /label="P1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(1067..1100)
                     /label="F3 (FRT)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1048..1067
                     /label="P2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1919..1953)
                     /label="J23116"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
       61 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
      121 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
      181 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
      241 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
      301 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
      361 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
      421 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
      481 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
      541 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
      601 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
      661 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgc
      721 atcgatggcc ccccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg
      781 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      841 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
      901 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag
      961 cagaaggcca tcctgacgga tggccttttt gcgtggccag tgccaagctt gcatgcagat
     1021 tgcagcatta cacgtcttga gcgattgTGT AGGCTGGAGC TGCTTCGAAG TTCCTATACT
     1081 aTtTgaAGAA TAGGAACTTC TTATTTGCCG ACTACCTTGG TGATCTCGCC TTTCACGTAG
     1141 TGGACAAATT CTTCCAACTG ATCTGCGCGC GAGGCCAAGC GATCTTCTTC TTGTCCAAGA
     1201 TAAGCCTGTC TAGCTTCAAG TATGACGGGC TGATACTGGG CCGGCAGGCG CTCCATTGCC
     1261 CAGTCGGCAG CGACATCCTT CGGCGCGATT TTGCCGGTTA CTGCGCTGTA CCAAATGCGG
     1321 GACAACGTAA GCACTACATT TCGCTCATCG CCAGCCCAGT CGGGCGGCGA GTTCCATAGC
     1381 GTTAAGGTTT CATTTAGCGC CTCAAATAGA TCCTGTTCAG GAACCGGATC AAAGAGTTCC
     1441 TCCGCCGCTG GACCTACCAA GGCAACGCTA TGTTCTCTTG CTTTTGTCAG CAAGATAGCC
     1501 AGATCAATGT CGATCGTGGC TGGCTCGAAG ATACCTGCAA GAATGTCATT GCGCTGCCAT
     1561 TCTCCAAATT GCAGTTCGCG CTTAGCTGGA TAACGCCACG GAATGATGTC GTCGTGCACA
     1621 ACAATGGTGA CTTCTACAGC GCGGAGAATC TCGCTCTCTC CAGGGGAAGC CGAAGTTTCC
     1681 AAAAGGTCGT TGATCAAAGC TCGCCGCGTT GTTTCATCAA GCCTTACGGT CACCGTAACC
     1741 AGCAAATCAA TATCACTGTG TGGCTTCAGG CCGCCATCCA CTGCGGAGCC GTACAAATGT
     1801 ACGGCCAGCA ACGTCGGTTC GAGATGGCGC TCGATGACGC CAACTACCTC TGATAGTTGA
     1861 GTCGATACTT CGGCGATCAC CGCTTCCCTC ATttagtatt tctcctcttt ctctagtagc
     1921 tagcatagtc cctaggactg agctagctgt caaGAAGTTC CTATACTaTt TgaAGAATAG
     1981 GAACTTCGAA CTGCAGGTCG ACGGATCCCC GGAATtaatt ctcatgtttg acagcttatc
     2041 actgatcagt gaattaatgg cgatgacgca tcctcacgat aatatccggg taggcgcaat
     2101 cactttcgtc tctactccgt tacaaagcga ggctgggtat ttcccggcct ttctgttatc
     2161 cgaaatccac tgaaagcaca gcggctggct gaggagataa ataataaacg aggggctgta
     2221 tgcacaaagc atcttctgtt gagttaagaa cgagtatcga gatggcacat agccttgctc
     2281 aaattggaat caggtttgtg ccaataccag tagaaacaga cgaagaagct agctttgcac
     2341 tggattgcga ggctttgcca tggctaattc ccatgtcagc cgttaagtgt tcctgtgtca
     2401 ctgaaaattg ctttgagagg ctctaagggc ttctcagtgc gttacatccc tggcttgttg
     2461 tccacaaccg ttaaacctta aaagctttaa aagccttata tattcttttt tttcttataa
     2521 aacttaaaac cttagaggct atttaagttg ctgatttata ttaattttat tgttcaaaca
     2581 tgagagctta gtacgtgaaa catgagagct tagtacgtta gccatgagag cttagtacgt
     2641 tagccatgag ggtttagttc gttaaacatg agagcttagt acgttaaaca tgagagctta
     2701 gtacgtgaaa catgagagct tagtacgtac tatcaacagg ttgaactgcg gatcttgcgg
     2761 ccgcaaaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2821 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2881 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2941 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     3001 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     3061 catccagtct attaattgtt gccgggaagc tagagtaag
//