LOCUS       FRP2350                 5411 bp ds-DNA     circular     06-JUN-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2422..2436
                     /label="tetRbinding"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1892..2128
                     /label="term_TEF1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2249..2263
                     /label="tetRbinding"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2754..2768
                     /label="tetRbinding"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             863..1891
                     /label="hyg B marker"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2731..2745
                     /label="tetRbinding"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     gap             2956..2979
                     /label="8G linker"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gap             2884..2955
                     /label="FLAG TAG|Unknown"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2384..2398
                     /label="tetRbinding"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        2151..2875
                     /label="PTeto7.1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        441..459
                     /label="T7_promoter Promoter|promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     2980..3000
                     /label="S4 primer site"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    484..862
                     /label="P_TEF1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(3018..3037)
                     /label="T3_promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2276..2290
                     /label="tetRbinding"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2357..2371
                     /label="tetRbinding"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
       61 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
      121 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
      181 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
      241 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
      301 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
      361 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
      421 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattggag ctcgatcctc
      481 gagagcttgc cttgtccccg ccgggtcacc cggccagcga catggaggcc cagaataccc
      541 tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc ccgtacattt
      601 agcccataca tccccatgta taatcatttg catccataca ttttgatggc cgcacggcgc
      661 gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca gggaaacgct cccctcacag
      721 acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt taggatttgc
      781 cactgaggtt cttctttcat atacttcctt ttaaaatctt gctaggatac agttctcaca
      841 tcacatccga acataaacaa ccatgggtaa aaagcctgaa ctcaccgcga cgtctgtcga
      901 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
      961 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     1021 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     1081 cccgattccg gaagtgcttg acattgggga gttcagcgag agcctgacct attgcatctc
     1141 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     1201 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     1261 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     1321 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     1381 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     1441 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     1501 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     1561 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     1621 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     1681 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     1741 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     1801 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     1861 ccccagcact cgtccgaggg caaaggaata atcagtactg acaataaaaa gattcttgtt
     1921 ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat
     1981 gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt
     2041 gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg
     2101 attcgatact aacgccgcca tccagtgttt aaacgaacta gtgcggccgc cagttcgagt
     2161 ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt agtgattttc
     2221 ctaactttat ttagtcaaaa aattagcctc tatcattgat agagtgtctg ggtgatctat
     2281 cattgataga gcatccacta attttaattc tgctgtaacc cgtacatgcc caaaataggg
     2341 ggcgggttac acagaatcta tcattgatag agtgtctggg tgatctatca ttgatagagc
     2401 atccactaaa tataatggag ctctatcatt gatagagcat ccactaaaaa aaagaatccc
     2461 agcaccaaaa tattgttttc ttcaccaacc atcagttcat aggtccattc tcttagcgca
     2521 actacagaga acaggggcac aaacaggcaa aaaacgggca caacctcaat ggagtgatgc
     2581 aacctgcctg gagtaaatga tgacacaagg caattgaccc acgcatgtat ctatctcatt
     2641 ttcttacacc ttctattacc ttctgctctc tctgatttgg aaaaagctga aaaaaaaggt
     2701 tgaaaccagt tccctgaaat tattccccta tctatcattg atagatataa atatctatca
     2761 ttgatagagt aattctgtaa atctatttct taaacttctt aaattctact tttatagtta
     2821 gtcttttttt tagttttaaa acaccaagaa cttagtttcg aataaacaca cataaacaaa
     2881 atggattata aagatgacga tgacaaggat tataaagatg acgatgacaa ggattataaa
     2941 gatgacgatg acaagggagg tggtggtggt ggaggcggtc cgacagagaa ttcatcgatg
     3001 ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     3061 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3121 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3181 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3241 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3301 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3361 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3421 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3481 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3541 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3601 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3661 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3721 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3781 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3841 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3901 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3961 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4021 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4081 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4141 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4201 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4261 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4321 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4381 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4441 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     4501 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     4561 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4621 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4681 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4741 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4801 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4861 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4921 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4981 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5041 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5101 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5161 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccaa tacccgactc
     5221 tatatgctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     5281 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     5341 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     5401 ggactccaac g
//