LOCUS       FRP2375                 4955 bp ds-DNA     circular     10-JAN-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(join(4532..4955,1..165))
                     /label="ori|rep_origin"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3444..3455
                     /label="RAP1binding"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(4179..4208)
                     /label="lac_promoter Promoter|promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(1238..1266)
                     /label="ampicillin_resistance_gene(b-lactamase)promoter Promoter|promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3456..3470
                     /label="tetRbinding"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3826..3840
                     /label="tetRbinding"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_binding    2391..2394
                     /label="misc_binding"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3818..3825
                     /label="TATAbox"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3336..3347
                     /label="RAP1binding"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3363..3373
                     /label="GCR1binding"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3494..3508
                     /label="tetRbinding"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     gap             4028..4051
                     /label="8G linker|Unknown"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3471..3481
                     /label="GCR1binding"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(4090..4109)
                     /label="T3_promoter Promoter|promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3321..3335
                     /label="tetRbinding"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3429..3443
                     /label="tetRbinding"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     gap             complement(4145..4181)
                     /label="lacI binding site|Unknown"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        3223..3947
                     /label="final_promoter|promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_binding    2947..2952
                     /label="misc_binding"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_binding    2701..2706
                     /label="misc_binding"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_binding    2419..2424
                     /label="misc_binding"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3509..3519
                     /label="GCR1binding"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2391..2963
                     /label="NatMX"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        1969..1987
                     /label="T7_promoter Promoter|promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     gap             1238..1266
                     /label="amp prom|Unknown"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3803..3817
                     /label="tetRbinding"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3348..3362
                     /label="tetRbinding"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     gap             3956..4027
                     /label="FLAG TAG|Unknown"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     4052..4072
                     /label="S4 primer site|primer_bind"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
       61 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      121 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      181 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      241 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      301 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      361 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
      421 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
      481 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
      541 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
      601 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
      661 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
      721 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
      781 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
      841 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
      901 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
      961 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1021 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1081 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1141 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1201 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1261 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1321 ccaccaatac ccgactctat atgctaaatt gtaagcgtta atattttgtt aaaattcgcg
     1381 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     1441 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     1501 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     1561 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     1621 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     1681 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     1741 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     1801 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     1861 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     1921 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca
     1981 ctatagggcg aattggagct cgatcctcga gagcttgcct tgtccccgcc gggtcacccg
     2041 gccagcgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg
     2101 gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca
     2161 tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctgcagacc
     2221 tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg
     2281 tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt
     2341 aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaacc atgggtacca
     2401 ctcttgacga cacggcttac cggtaccgca ccagtgtccc gggggacgcc gaggccatcg
     2461 aggcactgga tgggtccttc accaccgaca ccgtcttccg cgtcaccgcc accggggacg
     2521 gcttcaccct gcgggaggtg ccggtggacc cgcccctgac caaggtgttc cccgacgacg
     2581 aatcggacga cgaatcggac gacggggagg acggcgaccc ggactcccgg acgttcgtcg
     2641 cgtacgggga cgacggcgac ctggcgggct tcgtggtcgt ctcgtactcc ggctggaacc
     2701 gccggctgac cgtcgaggac atcgaggtcg ccccggagca ccgggggcac ggggtcgggc
     2761 gcgcgttgat ggggctcgcg acggagttcg cccgcgagcg gggcgccggg cacctctggc
     2821 tggaggtcac caacgtcaac gcaccggcga tccacgcgta ccggcggatg gggttcaccc
     2881 tctgcggcct ggacaccgcc ctgtacgacg gcaccgcctc ggacggcgag caggcgctct
     2941 acatgagcat gccctgcccc taatcagtac tgacaataaa aagattcttg ttttcaagaa
     3001 cttgtcattt gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt
     3061 gatttatatt ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa
     3121 agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata
     3181 ctaacgccgc catccagtgt ttaaacgaac tagtgcggcc gccagttcga gtttatcatt
     3241 atcaatactg ccatttcaaa gaatacgtaa ataattaata gtagtgattt tcctaacttt
     3301 atttagtcaa aaaattagcc tctatcattg atagagtgtc tgggtgatct atcattgata
     3361 gagcatccac taattttaat tctgctgtaa cccgtacatg cccaaaatag ggggcgggtt
     3421 acacagaatc tatcattgat agagtgtctg ggtgatctat cattgataga gcatccacta
     3481 aatataatgg agctctatca ttgatagagc atccactaaa aaaaagaatc ccagcaccaa
     3541 aatattgttt tcttcaccaa ccatcagttc ataggtccat tctcttagcg caactacaga
     3601 gaacaggggc acaaacaggc aaaaaacggg cacaacctca atggagtgat gcaacctgcc
     3661 tggagtaaat gatgacacaa ggcaattgac ccacgcatgt atctatctca ttttcttaca
     3721 ccttctatta ccttctgctc tctctgattt ggaaaaagct gaaaaaaaag gttgaaacca
     3781 gttccctgaa attattcccc tatctatcat tgatagatat aaatatctat cattgataga
     3841 gtaattctgt aaatctattt cttaaacttc ttaaattcta cttttatagt tagtcttttt
     3901 tttagtttta aaacaccaag aacttagttt cgaataaaca cacataaaca aaatggatta
     3961 taaagatgac gatgacaagg attataaaga tgacgatgac aaggattata aagatgacga
     4021 tgacaaggga ggtggtggtg gtggaggcgg tccgacagag aattcatcga tgggtaccca
     4081 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     4141 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4201 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4261 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     4321 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     4381 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4441 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4501 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4561 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4621 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4681 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4741 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4801 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4861 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4921 gcggtgctac agagttcttg aagtggtggc ctaac
//