LOCUS       pRS416-PTEF1-MCP-no_intron           5877 bp    DNA     linear       13-MAY-2019
FEATURES             Location/Qualifiers
     promoter        2997..3211
                     /label=URA3_promoter
     rep_origin      complement(440..1059)
                     /label=pBR322_origin
     gene            complement(1214..2074)
                     /dnas_title="Ampicillin"
                     /label=Ampicillin
                     /gene="Ampicillin"
     misc_feature    2206..2724
                     /label=CEN6_ARS4
     rep_origin      complement(4278..4584)
                     /label=f1_origin
     promoter        4804..5217
                     /dnas_title="TEF1_promoter"
                     /note="TEF1"
     gene            5218..5637
                     /dnas_title="FLAG-MCP"
                     /note=""
     terminator      5638..5877
                     /dnas_title="CYC1_terminator"
                     /note="CYC1"
     exon            3212..4015
                     /dnas_title="URA3_no_intron"
                     /note=""
     source          1..5877
                     /dnas_title="pRS416-PTEF1-MCP-no_intron"
ORIGIN      
        1 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
       61 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatagga gccggaagca
      121 taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt gcgttgcgct
      181 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      241 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      301 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      361 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      421 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      481 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      541 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      601 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      661 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      721 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      781 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      841 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      901 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      961 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1021 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1081 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1141 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1201 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1261 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1321 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1381 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1441 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1501 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1561 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1621 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1681 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1741 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1801 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1861 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1921 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1981 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2041 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     2101 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2161 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc ttttcatcac
     2221 gtgctataaa aataattata atttaaattt tttaatataa atatataaat taaaaataga
     2281 aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct
     2341 agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg
     2401 ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat
     2461 tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat
     2521 atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca
     2581 ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa
     2641 taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt
     2701 aagttacagg caagcgatcc gtcctaagaa accattatta tcatgacatt aacctataaa
     2761 aataggcgta tcacgaggcc ctttcgtcga attcgcgcgt ttcggtgatg acggtgaaaa
     2821 cctctgacac atgcagctcc cggagatctc ggtcacagct tgtctgtaag cggatgccgg
     2881 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     2941 ctatgcggca tcagagcaga ttgtactgag agtgcaccat accacagctt ttcaattcaa
     3001 ttcatcattt tttttttatt cttttttttg atttcggttt ctttgaaatt tttttgattc
     3061 ggtaatctcc gaacagaagg aagaacgaag gaaggagcac agacttagat tggtatatat
     3121 acgcatatgt agtgttgaag aaacatgaaa ttgcccagta ttcttaaccc aactgcacag
     3181 aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa gctacatata aggaacgtgc
     3241 tgctactcat cctagtcctg ttgctgccaa gctatttaat atcatgcacg aaaagcaaac
     3301 aaacttgtgt gcttcattgg atgttcgtac caccaaggaa ttactggagt tagttgaagc
     3361 attaggtccc aaaatttgtt tactaaaaac acatgtggat atcttgactg atttttccat
     3421 ggagggcaca gttaagccgc taaaggcatt atccgccaag tacaattttt tactcttcga
     3481 agacagaaaa tttgctgaca ttggtaatac agtcaaattg cagtactctg cgggtgtata
     3541 cagaatagca gaatgggcag acattacgaa tgcacacggt gtggtgggcc caggtattgt
     3601 tagcggtttg aagcaggcgg cagaagaagt aacaaaggaa cctagaggcc ttttgatgtt
     3661 agcagaattg tcatgcaagg gctccctatc tactggagaa tatactaagg gtactgttga
     3721 cattgcgaag agcgacaaag attttgttat cggctttatt gctcaaagag acatgggtgg
     3781 aagagatgaa ggttacgatt ggttgattat gacacccggt gtgggtttag atgacaaggg
     3841 TgaTgcattg ggtcaacagt atagaaccgt ggatgatgtg gtctctacag gatctgacat
     3901 tattattgtt ggaagaggac tatttgcaaa gggaagggat gctaaggtag agggtgaacg
     3961 ttacagaaaa gcaggctggg aagcatattt gagaagatgc ggccagcaaa actaaaaaac
     4021 tgtattataa gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa tttaattata
     4081 tcagttatta ccctatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     4141 tcaggaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     4201 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     4261 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     4321 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     4381 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     4441 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     4501 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     4561 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcgcgccatt cgccattcag
     4621 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     4681 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     4741 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
     4801 taccatagct tcaaaatgtt tctactcctt ttttactctt ccagattttc tcggactccg
     4861 cgcatcgccg taccacttca aaacacccaa gcacagcata ctaaatttcc cctctttctt
     4921 cctctagggt gtcgttaatt acccgtacta aaggtttgga aaagaaaaaa gagaccgcct
     4981 cgtttctttt tcttcgtcga aaaaggcaat aaaaattttt atcacgtttc tttttcttga
     5041 aaattttttt tttgattttt ttctctttcg atgacctccc attgatattt aagttaataa
     5101 acggtcttca atttctcaag tttcagtttc atttttcttg ttctattaca acttttttta
     5161 cttcttgctc attagaaaga aagcatagca atctaatcta agttttctag aactagtatg
     5221 gactacaagg acgacgatga caagatggct tctaacttta ctcagttcgt tctcgtcgac
     5281 aatggcggaa ctggcgacgt gactgtcgcc ccaagcaact tcgctaacgg ggtcgctgaa
     5341 tggatcagct ctaactcgcg atcacaggct tacaaagtaa cctgtagcgt tcgtcagagc
     5401 tctgcgcaga atcgcaaata caccatcaaa gtcgaggtgc ctaaagtggc aacccagact
     5461 gttggtggtg aagagcttcc tgtagccgga tggagatctt acttaaatat ggaactaacc
     5521 attccaattt tcgccacgaa ttccgactgc gagcttattg ttaaggcaat gcaaggtctc
     5581 ctaaaagatg gaaacccgat tccctcggcc atcgcggcca actccggcat ctactagatt
     5641 agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg
     5701 agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag
     5761 aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa
     5821 cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttg
//

