LOCUS       pRS416-PTEF1-MCP-ECM33_intron                6207 bp    DNA     linear       13-MAY-2019
FEATURES             Location/Qualifiers
     promoter        2997..3211
                     /label=URA3_promoter
     rep_origin      complement(440..1059)
                     /label=pBR322_origin
     gene            complement(1214..2074)
                     /dnas_title="Ampicillin"
                     /label=Ampicillin
                     /gene="Ampicillin"
     misc_feature    2206..2724
                     /label=CEN6_ARS4
     rep_origin      complement(4608..4914)
                     /label=f1_origin
     promoter        5134..5547
                     /dnas_title="TEF1_promoter"
                     /note="TEF1"
     gene            5548..5967
                     /dnas_title="FLAG-MCP"
                     /note=""
     terminator      5968..6207
                     /dnas_title="CYC1_terminator"
                     /note="CYC1"
     exon            3212..3263
                     /dnas_title="URA3_exon1"
                     /note=""
     exon            3594..4345
                     /dnas_title="URA3_exon2"
                     /note=""
     intron          3264..3593
                     /note="ECM33_intron"
     source          1..6207
                     /dnas_title="pRS416-PTEF1-MCP-ECM33_intron"
ORIGIN      
        1 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
       61 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatagga gccggaagca
      121 taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt gcgttgcgct
      181 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      241 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      301 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      361 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      421 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      481 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      541 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      601 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      661 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      721 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      781 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      841 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      901 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      961 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1021 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1081 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1141 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1201 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1261 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1321 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1381 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1441 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1501 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1561 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1621 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1681 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1741 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1801 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1861 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1921 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1981 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2041 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     2101 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2161 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc ttttcatcac
     2221 gtgctataaa aataattata atttaaattt tttaatataa atatataaat taaaaataga
     2281 aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct
     2341 agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg
     2401 ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat
     2461 tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat
     2521 atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca
     2581 ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa
     2641 taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt
     2701 aagttacagg caagcgatcc gtcctaagaa accattatta tcatgacatt aacctataaa
     2761 aataggcgta tcacgaggcc ctttcgtcga attcgcgcgt ttcggtgatg acggtgaaaa
     2821 cctctgacac atgcagctcc cggagatctc ggtcacagct tgtctgtaag cggatgccgg
     2881 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     2941 ctatgcggca tcagagcaga ttgtactgag agtgcaccat accacagctt ttcaattcaa
     3001 ttcatcattt tttttttatt cttttttttg atttcggttt ctttgaaatt tttttgattc
     3061 ggtaatctcc gaacagaagg aagaacgaag gaaggagcac agacttagat tggtatatat
     3121 acgcatatgt agtgttgaag aaacatgaaa ttgcccagta ttcttaaccc aactgcacag
     3181 aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa gctacatata aggaacgtgc
     3241 tgctactcat cctagtcctg ttgGTATGTA CACATTCTCC TTTTTTTTCA TCTTTTTTTC
     3301 TTTATTTGCC TCTTTCCTCA CAAATCTCGA GTAGATTCGT GGTCCTCTTC ATTCTTTTCT
     3361 TTTTTCTTTG TCGATTACTG GGCTTTTTTT CATAGGTCTC GATTGACGCG GACGGACAAT
     3421 GCGAAAAAAA AAAATTTCCA AAAGAGGAAA CGGGTTTCGA GATCTCGTGA GGATGGTTTT
     3481 GGAATATTCT TCAATATTTC TGCCTGTCAT AGGATTAGGG CGAGTAATGA AACAGAATAA
     3541 TACATGTATA AATCGATCGG GAATACTAAC ACTACTTTTC TTTATCTAAG CAGctgccaa
     3601 gctatttaat atcatgcacg aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac
     3661 caccaaggaa ttactggagt tagttgaagc attaggtccc aaaatttgtt tactaaaaac
     3721 acatgtggat atcttgactg atttttccat ggagggcaca gttaagccgc taaaggcatt
     3781 atccgccaag tacaattttt tactcttcga agacagaaaa tttgctgaca ttggtaatac
     3841 agtcaaattg cagtactctg cgggtgtata cagaatagca gaatgggcag acattacgaa
     3901 tgcacacggt gtggtgggcc caggtattgt tagcggtttg aagcaggcgg cagaagaagt
     3961 aacaaaggaa cctagaggcc ttttgatgtt agcagaattg tcatgcaagg gctccctatc
     4021 tactggagaa tatactaagg gtactgttga cattgcgaag agcgacaaag attttgttat
     4081 cggctttatt gctcaaagag acatgggtgg aagagatgaa ggttacgatt ggttgattat
     4141 gacacccggt gtgggtttag atgacaaggg TgaTgcattg ggtcaacagt atagaaccgt
     4201 ggatgatgtg gtctctacag gatctgacat tattattgtt ggaagaggac tatttgcaaa
     4261 gggaagggat gctaaggtag agggtgaacg ttacagaaaa gcaggctggg aagcatattt
     4321 gagaagatgc ggccagcaaa actaaaaaac tgtattataa gtaaatgcat gtatactaaa
     4381 ctcacaaatt agagcttcaa tttaattata tcagttatta ccctatgcgg tgtgaaatac
     4441 cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaaacgtta atattttgtt
     4501 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     4561 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     4621 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     4681 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     4741 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     4801 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     4861 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     4921 acagggcgcg tcgcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     4981 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     5041 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc
     5101 gtaatacgac tcactatagg gcgaattggg taccatagct tcaaaatgtt tctactcctt
     5161 ttttactctt ccagattttc tcggactccg cgcatcgccg taccacttca aaacacccaa
     5221 gcacagcata ctaaatttcc cctctttctt cctctagggt gtcgttaatt acccgtacta
     5281 aaggtttgga aaagaaaaaa gagaccgcct cgtttctttt tcttcgtcga aaaaggcaat
     5341 aaaaattttt atcacgtttc tttttcttga aaattttttt tttgattttt ttctctttcg
     5401 atgacctccc attgatattt aagttaataa acggtcttca atttctcaag tttcagtttc
     5461 atttttcttg ttctattaca acttttttta cttcttgctc attagaaaga aagcatagca
     5521 atctaatcta agttttctag aactagtatg gactacaagg acgacgatga caagatggct
     5581 tctaacttta ctcagttcgt tctcgtcgac aatggcggaa ctggcgacgt gactgtcgcc
     5641 ccaagcaact tcgctaacgg ggtcgctgaa tggatcagct ctaactcgcg atcacaggct
     5701 tacaaagtaa cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata caccatcaaa
     5761 gtcgaggtgc ctaaagtggc aacccagact gttggtggtg aagagcttcc tgtagccgga
     5821 tggagatctt acttaaatat ggaactaacc attccaattt tcgccacgaa ttccgactgc
     5881 gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat tccctcggcc
     5941 atcgcggcca actccggcat ctactagatt agttatgtca cgcttacatt cacgccctcc
     6001 ccccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat
     6061 ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt
     6121 ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt
     6181 tttgggacgc tcgaaggctt taatttg
//

