LOCUS pRS416-PTEF1-MCP-5P-ECM33_intron 6296 bp DNA linear 15-MAY-2019 FEATURES Location/Qualifiers promoter 2997..3211 /label=URA3_promoter rep_origin complement(440..1059) /label=pBR322_origin gene complement(1214..2074) /dnas_title="Ampicillin" /label=Ampicillin /gene="Ampicillin" misc_feature 2206..2724 /label=CEN6_ARS4 rep_origin complement(4697..5003) /label=f1_origin promoter 5223..5636 /dnas_title="TEF1_promoter" /note="TEF1" gene 5637..6056 /dnas_title="FLAG-MCP" /note="" terminator 6057..6296 /dnas_title="CYC1_terminator" /note="CYC1" exon 3212..3263 /dnas_title="URA3_exon1" /note="" exon 3683..4434 /dnas_title="URA3_exon2" /note="" intron 3264..3682 /note="5P_intron" source 1..6296 /dnas_title="pRS416-PTEF1-MCP-5P-ECM33_intron" ORIGIN 1 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc 61 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatagga gccggaagca 121 taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt gcgttgcgct 181 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 241 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 301 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 361 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 421 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 481 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 541 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 601 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 661 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 721 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 781 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 841 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 901 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 961 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 1021 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 1081 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 1141 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 1201 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 1261 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 1321 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 1381 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 1441 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 1501 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 1561 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 1621 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 1681 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 1741 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 1801 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 1861 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 1921 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 1981 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 2041 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 2101 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 2161 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc ttttcatcac 2221 gtgctataaa aataattata atttaaattt tttaatataa atatataaat taaaaataga 2281 aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct 2341 agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg 2401 ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat 2461 tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat 2521 atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca 2581 ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa 2641 taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt 2701 aagttacagg caagcgatcc gtcctaagaa accattatta tcatgacatt aacctataaa 2761 aataggcgta tcacgaggcc ctttcgtcga attcgcgcgt ttcggtgatg acggtgaaaa 2821 cctctgacac atgcagctcc cggagatctc ggtcacagct tgtctgtaag cggatgccgg 2881 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 2941 ctatgcggca tcagagcaga ttgtactgag agtgcaccat accacagctt ttcaattcaa 3001 ttcatcattt tttttttatt cttttttttg atttcggttt ctttgaaatt tttttgattc 3061 ggtaatctcc gaacagaagg aagaacgaag gaaggagcac agacttagat tggtatatat 3121 acgcatatgt agtgttgaag aaacatgaaa ttgcccagta ttcttaaccc aactgcacag 3181 aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa gctacatata aggaacgtgc 3241 tgctactcat cctagtcctg ttgGTATGTA CACATTCTCC TTTTTTTTCA TCTTTTTTTC 3301 TTTATTTGCC TCTTTCCTCA CAgggatttt tgacgtcgcA CATGAGGATC ACCCATGTgc 3361 gacgtctttt tcacgagcgA CATGAGGATC ACCCATGTcg ctcgtgttcc cAATCTCGAG 3421 TAGATTCGTG GTCCTCTTCA TTCTTTTCTT TTTTCTTTGT CGATTACTGG GCTTTTTTTC 3481 ATAGGTCTCG ATTGACGCGG ACGGACAATG CGAAAAAAAA AAATTTCCAA AAGAGGAAAC 3541 GGGTTTCGAG ATCTCGTGAG GATGGTTTTG GAATATTCTT CAATATTTCT GCCTGTCATA 3601 GGATTAGGGC GAGTAATGAA ACAGAATAAT ACATGTATAA ATCGATCGGG AATACTAACA 3661 CTACTTTTCT TTATCTAAGC AGctgccaag ctatttaata tcatgcacga aaagcaaaca 3721 aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt agttgaagca 3781 ttaggtccca aaatttgttt actaaaaaca catgtggata tcttgactga tttttccatg 3841 gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt actcttcgaa 3901 gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc gggtgtatac 3961 agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc aggtattgtt 4021 agcggtttga agcaggcggc agaagaagta acaaaggaac ctagaggcct tttgatgtta 4081 gcagaattgt catgcaaggg ctccctatct actggagaat atactaaggg tactgttgac 4141 attgcgaaga gcgacaaaga ttttgttatc ggctttattg ctcaaagaga catgggtgga 4201 agagatgaag gttacgattg gttgattatg acacccggtg tgggtttaga tgacaagggT 4261 gaTgcattgg gtcaacagta tagaaccgtg gatgatgtgg tctctacagg atctgacatt 4321 attattgttg gaagaggact atttgcaaag ggaagggatg ctaaggtaga gggtgaacgt 4381 tacagaaaag caggctggga agcatatttg agaagatgcg gccagcaaaa ctaaaaaact 4441 gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat ttaattatat 4501 cagttattac cctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 4561 caggaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc 4621 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 4681 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 4741 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 4801 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg 4861 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 4921 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 4981 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cgcgccattc gccattcagg 5041 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg 5101 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga 5161 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt 5221 accatagctt caaaatgttt ctactccttt tttactcttc cagattttct cggactccgc 5281 gcatcgccgt accacttcaa aacacccaag cacagcatac taaatttccc ctctttcttc 5341 ctctagggtg tcgttaatta cccgtactaa aggtttggaa aagaaaaaag agaccgcctc 5401 gtttcttttt cttcgtcgaa aaaggcaata aaaattttta tcacgtttct ttttcttgaa 5461 aatttttttt ttgatttttt tctctttcga tgacctccca ttgatattta agttaataaa 5521 cggtcttcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa ctttttttac 5581 ttcttgctca ttagaaagaa agcatagcaa tctaatctaa gttttctaga actagtatgg 5641 actacaagga cgacgatgac aagatggctt ctaactttac tcagttcgtt ctcgtcgaca 5701 atggcggaac tggcgacgtg actgtcgccc caagcaactt cgctaacggg gtcgctgaat 5761 ggatcagctc taactcgcga tcacaggctt acaaagtaac ctgtagcgtt cgtcagagct 5821 ctgcgcagaa tcgcaaatac accatcaaag tcgaggtgcc taaagtggca acccagactg 5881 ttggtggtga agagcttcct gtagccggat ggagatctta cttaaatatg gaactaacca 5941 ttccaatttt cgccacgaat tccgactgcg agcttattgt taaggcaatg caaggtctcc 6001 taaaagatgg aaacccgatt ccctcggcca tcgcggccaa ctccggcatc tactagatta 6061 gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga 6121 gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga 6181 acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac 6241 attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttg //