LOCUS       pRS416-PTEF1-MCP-3P-ECM33_intron                 6296 bp    DNA     linear       20-MAY-2019
FEATURES             Location/Qualifiers
     promoter        2997..3211
                     /label=URA3_promoter
     rep_origin      complement(440..1059)
                     /label=pBR322_origin
     gene            complement(1214..2074)
                     /dnas_title="Ampicillin"
                     /label=Ampicillin
                     /gene="Ampicillin"
     misc_feature    2206..2724
                     /label=CEN6_ARS4
     rep_origin      complement(4697..5003)
                     /label=f1_origin
     promoter        5223..5636
                     /dnas_title="TEF1_promoter"
                     /note="TEF1"
     gene            5637..6056
                     /dnas_title="FLAG-MCP"
                     /note=""
     terminator      6057..6296
                     /dnas_title="CYC1_terminator"
                     /note="CYC1"
     exon            3212..3263
                     /dnas_title="URA3_exon1"
                     /note=""
     exon            3683..4434
                     /dnas_title="URA3_exon2"
                     /note=""
     intron          3264..3682
                     /note="3P_intron"
     source          1..6296
                     /dnas_title="pRS416-PTEF1-MCP-3P-ECM33_intron"
ORIGIN      
        1 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
       61 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatagga gccggaagca
      121 taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt gcgttgcgct
      181 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      241 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      301 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      361 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      421 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      481 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      541 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      601 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      661 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      721 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      781 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      841 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      901 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      961 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1021 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1081 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1141 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1201 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1261 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1321 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1381 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1441 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1501 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1561 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1621 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1681 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1741 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1801 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1861 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1921 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1981 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2041 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     2101 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2161 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc ttttcatcac
     2221 gtgctataaa aataattata atttaaattt tttaatataa atatataaat taaaaataga
     2281 aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct
     2341 agggggatcg ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg
     2401 ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat
     2461 tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat
     2521 atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca
     2581 ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa
     2641 taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt
     2701 aagttacagg caagcgatcc gtcctaagaa accattatta tcatgacatt aacctataaa
     2761 aataggcgta tcacgaggcc ctttcgtcga attcgcgcgt ttcggtgatg acggtgaaaa
     2821 cctctgacac atgcagctcc cggagatctc ggtcacagct tgtctgtaag cggatgccgg
     2881 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     2941 ctatgcggca tcagagcaga ttgtactgag agtgcaccat accacagctt ttcaattcaa
     3001 ttcatcattt tttttttatt cttttttttg atttcggttt ctttgaaatt tttttgattc
     3061 ggtaatctcc gaacagaagg aagaacgaag gaaggagcac agacttagat tggtatatat
     3121 acgcatatgt agtgttgaag aaacatgaaa ttgcccagta ttcttaaccc aactgcacag
     3181 aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa gctacatata aggaacgtgc
     3241 tgctactcat cctagtcctg ttgGTATGTA CACATTCTCC TTTTTTTTCA TCTTTTTTTC
     3301 TTTATTTGCC TCTTTCCTCA CAAATCTCGA GTAGATTCGT GGTCCTCTTC ATTCTTTTCT
     3361 TTTTTCTTTG TCGATTACTG GGCTTTTTTT CATAGGTCTC GATTGACGCG GACGGACAAT
     3421 GCGAAAAAAA AAAATTTCCA AAAGAGGAAA CGGGTTTCGA GATCTCGTGA GGATGGTTTT
     3481 GGAATATTCg ggatttttga cgtcgcACAT GAGGATCACC CATGTgcgac gtctttttca
     3541 cgagcgACAT GAGGATCACC CATGTcgctc gtgttcccTT CAATATTTCT GCCTGTCATA
     3601 GGATTAGGGC GAGTAATGAA ACAGAATAAT ACATGTATAA ATCGATCGGG AATACTAACA
     3661 CTACTTTTCT TTATCTAAGC AGctgccaag ctatttaata tcatgcacga aaagcaaaca
     3721 aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt agttgaagca
     3781 ttaggtccca aaatttgttt actaaaaaca catgtggata tcttgactga tttttccatg
     3841 gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt actcttcgaa
     3901 gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc gggtgtatac
     3961 agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc aggtattgtt
     4021 agcggtttga agcaggcggc agaagaagta acaaaggaac ctagaggcct tttgatgtta
     4081 gcagaattgt catgcaaggg ctccctatct actggagaat atactaaggg tactgttgac
     4141 attgcgaaga gcgacaaaga ttttgttatc ggctttattg ctcaaagaga catgggtgga
     4201 agagatgaag gttacgattg gttgattatg acacccggtg tgggtttaga tgacaagggT
     4261 gaTgcattgg gtcaacagta tagaaccgtg gatgatgtgg tctctacagg atctgacatt
     4321 attattgttg gaagaggact atttgcaaag ggaagggatg ctaaggtaga gggtgaacgt
     4381 tacagaaaag caggctggga agcatatttg agaagatgcg gccagcaaaa ctaaaaaact
     4441 gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat ttaattatat
     4501 cagttattac cctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     4561 caggaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     4621 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     4681 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     4741 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     4801 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     4861 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     4921 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     4981 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cgcgccattc gccattcagg
     5041 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     5101 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     5161 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt
     5221 accatagctt caaaatgttt ctactccttt tttactcttc cagattttct cggactccgc
     5281 gcatcgccgt accacttcaa aacacccaag cacagcatac taaatttccc ctctttcttc
     5341 ctctagggtg tcgttaatta cccgtactaa aggtttggaa aagaaaaaag agaccgcctc
     5401 gtttcttttt cttcgtcgaa aaaggcaata aaaattttta tcacgtttct ttttcttgaa
     5461 aatttttttt ttgatttttt tctctttcga tgacctccca ttgatattta agttaataaa
     5521 cggtcttcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa ctttttttac
     5581 ttcttgctca ttagaaagaa agcatagcaa tctaatctaa gttttctaga actagtatgg
     5641 actacaagga cgacgatgac aagatggctt ctaactttac tcagttcgtt ctcgtcgaca
     5701 atggcggaac tggcgacgtg actgtcgccc caagcaactt cgctaacggg gtcgctgaat
     5761 ggatcagctc taactcgcga tcacaggctt acaaagtaac ctgtagcgtt cgtcagagct
     5821 ctgcgcagaa tcgcaaatac accatcaaag tcgaggtgcc taaagtggca acccagactg
     5881 ttggtggtga agagcttcct gtagccggat ggagatctta cttaaatatg gaactaacca
     5941 ttccaatttt cgccacgaat tccgactgcg agcttattgt taaggcaatg caaggtctcc
     6001 taaaagatgg aaacccgatt ccctcggcca tcgcggccaa ctccggcatc tactagatta
     6061 gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga
     6121 gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga
     6181 acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac
     6241 attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttg
//

