LOCUS       pRS416-ACT1_intron               5087 bp    DNA     linear       20-MAY-2019
FEATURES             Location/Qualifiers
     promoter        2985..3199
                     /label=URA3_promoter
     rep_origin      complement(428..1047)
                     /label=pBR322_origin
     gene            complement(1202..2062)
                     /dnas_title="Ampicillin"
                     /label=Ampicillin
                     /gene="Ampicillin"
     misc_feature    2194..2712
                     /label=CEN6_ARS4
     rep_origin      complement(4575..4881)
                     /label=f1_origin
     exon            3200..3251
                     /dnas_title="URA3_exon1"
                     /note=""
     exon            3561..4312
                     /dnas_title="URA3_exon2"
                     /note=""
     intron          3252..3560
                     /note="ACT1_intron"
     source          1..5087
                     /dnas_title="pRS416-ACT1_intron"
ORIGIN      
        1 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
       61 gaaattgtta tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag
      121 cctggggtgc ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt
      181 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      241 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      301 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      361 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      421 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      481 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      541 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      601 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      661 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      721 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      781 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      841 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
      901 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      961 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1021 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1081 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1141 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1201 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1261 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1321 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1381 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1441 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1501 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1561 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1621 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1681 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1741 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1801 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1861 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     1921 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     1981 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2041 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2101 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2161 ttccgcgcac atttccccga aaagtgccac ctgggtcctt ttcatcacgt gctataaaaa
     2221 taattataat ttaaattttt taatataaat atataaatta aaaatagaaa gtaaaaaaag
     2281 aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa aagactctag ggggatcgcc
     2341 aacaaatact accttttatc ttgctcttcc tgctctcagg tattaatgcc gaattgtttc
     2401 atcttgtctg tgtagaagac cacacacgaa aatcctgtga ttttacattt tacttatcgt
     2461 taatcgaatg tatatctatt taatctgctt ttcttgtcta ataaatatat atgtaaagta
     2521 cgctttttgt tgaaattttt taaacctttg tttatttttt tttcttcatt ccgtaactct
     2581 tctaccttct ttatttactt tctaaaatcc aaatacaaaa cataaaaata aataaacaca
     2641 gagtaaattc ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa gttacaggca
     2701 agcgatccgt cctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2761 acgaggccct ttcgtcgaat tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     2821 gcagctcccg gagatctcgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     2881 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     2941 agagcagatt gtactgagag tgcaccatac cacagctttt caattcaatt catcattttt
     3001 tttttattct tttttttgat ttcggtttct ttgaaatttt tttgattcgg taatctccga
     3061 acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac gcatatgtag
     3121 tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa caaaaacctg
     3181 caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg ctactcatcc
     3241 tagtcctgtt gGTATGTTCT AGCGCTTGCA CCATCCCATT TAACTGTAAG AAGAATTGCA
     3301 CGGTCCCAAT TGCTCGAGAG ATTTCTCTTT TACCTTTTTT TACTATTTTT CACTCTCCCA
     3361 TAACCTCCTA TATTGACTGA TCTGTAATAA CCACGATATT ATTGGAATAA ATAGGGGCTT
     3421 GAAATTTGGA AAAAAAAAAA AAACTGAAAT ATTTTCGTGA TAAGTGATAG TGATATTCTT
     3481 CTTTTATTTG CTACTGTTAC TAAGTCTCAT GTACTAACAT CGATTGCTTC ATTCTTTTTG
     3541 TTGCTATATT ATATGTTTAG ctgccaagct atttaatatc atgcacgaaa agcaaacaaa
     3601 cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag ttgaagcatt
     3661 aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt tttccatgga
     3721 gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac tcttcgaaga
     3781 cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg gtgtatacag
     3841 aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag gtattgttag
     3901 cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt tgatgttagc
     3961 agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta ctgttgacat
     4021 tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca tgggtggaag
     4081 agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg acaagggTga
     4141 Tgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat ctgacattat
     4201 tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta
     4261 cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact aaaaaactgt
     4321 attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt aattatatca
     4381 gttattaccc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     4441 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     4501 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     4561 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     4621 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     4681 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     4741 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     4801 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     4861 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
     4921 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     4981 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     5041 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagg
//

