LOCUS       pRS416-RPL27B_intron             5162 bp    DNA     linear       20-MAY-2019
FEATURES             Location/Qualifiers
     promoter        2985..3199
                     /label=URA3_promoter
     rep_origin      complement(428..1047)
                     /label=pBR322_origin
     gene            complement(1202..2062)
                     /dnas_title="Ampicillin"
                     /label=Ampicillin
                     /gene="Ampicillin"
     misc_feature    2194..2712
                     /label=CEN6_ARS4
     rep_origin      complement(4650..4956)
                     /label=f1_origin
     exon            3200..3251
                     /dnas_title="URA3_exon1"
                     /note=""
     exon            3636..4387
                     /dnas_title="URA3_exon2"
                     /note=""
     intron          3252..3635
                     /note="RPL27B_intron"
     source          1..5162
                     /dnas_title="pRS416-RPL27B_intron"
ORIGIN      
        1 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
       61 gaaattgtta tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag
      121 cctggggtgc ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt
      181 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      241 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      301 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      361 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      421 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      481 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      541 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      601 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      661 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      721 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      781 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      841 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
      901 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      961 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1021 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1081 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1141 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1201 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1261 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1321 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1381 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1441 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1501 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1561 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1621 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1681 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1741 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1801 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1861 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     1921 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     1981 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2041 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2101 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2161 ttccgcgcac atttccccga aaagtgccac ctgggtcctt ttcatcacgt gctataaaaa
     2221 taattataat ttaaattttt taatataaat atataaatta aaaatagaaa gtaaaaaaag
     2281 aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa aagactctag ggggatcgcc
     2341 aacaaatact accttttatc ttgctcttcc tgctctcagg tattaatgcc gaattgtttc
     2401 atcttgtctg tgtagaagac cacacacgaa aatcctgtga ttttacattt tacttatcgt
     2461 taatcgaatg tatatctatt taatctgctt ttcttgtcta ataaatatat atgtaaagta
     2521 cgctttttgt tgaaattttt taaacctttg tttatttttt tttcttcatt ccgtaactct
     2581 tctaccttct ttatttactt tctaaaatcc aaatacaaaa cataaaaata aataaacaca
     2641 gagtaaattc ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa gttacaggca
     2701 agcgatccgt cctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2761 acgaggccct ttcgtcgaat tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     2821 gcagctcccg gagatctcgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     2881 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     2941 agagcagatt gtactgagag tgcaccatac cacagctttt caattcaatt catcattttt
     3001 tttttattct tttttttgat ttcggtttct ttgaaatttt tttgattcgg taatctccga
     3061 acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac gcatatgtag
     3121 tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa caaaaacctg
     3181 caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg ctactcatcc
     3241 tagtcctgtt gGTACGTAAA TTTAACAGAG CAAACGCTCT AATTAAGATA TCGAATAAAA
     3301 GGGGACTTTC AGGTGCATAA GATGGGAAAT TGTACAATCT GAGGGACAAA CAATATGGAA
     3361 AGCAGTACAT GATTGTATTG TCATTGTAGG AGGTACCAAT TGTGCCAGTA AAAAAGAGAG
     3421 GGCCCGGGGA GCACCACATT CACGACCAAT CGTGTCGATC TGCAAAAAAG GCACATGGAA
     3481 AATGTAATTT ATCAGTGTTC AACACTGAGT TGAACAGATA GCTATCTAAT CAATGTTAAC
     3541 TTTCCAGGGA ACAAAAAGCT AAATCCACTT CTCTTTTATT TCAAAATATC ATTAGAAATA
     3601 GAAAAATTTA CTAACAAATT TTCGTATTAT CGTAGctgcc aagctattta atatcatgca
     3661 cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga
     3721 gttagttgaa gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac
     3781 tgatttttcc atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt
     3841 tttactcttc gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc
     3901 tgcgggtgta tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg
     3961 cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg
     4021 ccttttgatg ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa
     4081 gggtactgtt gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag
     4141 agacatgggt ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt
     4201 agatgacaag ggTgaTgcat tgggtcaaca gtatagaacc gtggatgatg tggtctctac
     4261 aggatctgac attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt
     4321 agagggtgaa cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca
     4381 aaactaaaaa actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc
     4441 aatttaatta tatcagttat taccctatgc ggtgtgaaat accgcacaga tgcgtaagga
     4501 gaaaataccg catcaggaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt
     4561 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     4621 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     4681 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     4741 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     4801 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     4861 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     4921 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcgcgcca
     4981 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     5041 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     5101 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata
     5161 gg
//

