LOCUS pRS426-ECM33_stable 6061 bp DNA linear 21-MAY-2019 FEATURES Location/Qualifiers promoter 3938..4152 /label=URA3_promoter rep_origin complement(428..1047) /label=pBR322_origin gene complement(1202..2062) /dnas_title="Ampicillin" /label=Ampicillin /gene="Ampicillin" rep_origin complement(5549..5855) /label=f1_origin exon 4153..4204 /dnas_title="URA3_exon1" /note="" exon 4535..5286 /dnas_title="URA3_exon2" /note="" intron 4205..4534 /dnas_title="ECM33_intron (stable, WT)" /note="ECM33_intron (stable, WT)" misc_feature 2194..3665 /note="2micron" source 1..6061 /dnas_title="pRS426-ECM33_stable" ORIGIN 1 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt 61 gaaattgtta tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag 121 cctggggtgc ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt 181 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 241 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 301 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 361 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 421 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 481 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 541 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 601 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 661 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 721 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 781 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 841 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 901 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 961 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 1021 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 1081 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 1141 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 1201 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 1261 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 1321 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 1381 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 1441 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 1501 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 1561 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 1621 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 1681 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 1741 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 1801 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 1861 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 1921 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 1981 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 2041 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 2101 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 2161 ttccgcgcac atttccccga aaagtgccac ctgCTTATCG ATGATAAGCT GTCAAAGATG 2221 AGAATTAATT CCACGGACTA TAGACTATAC TAGATACTCC GTCTACTGTA CGATACACTT 2281 CCGCTCAGGT CCTTGTCCTT TAACGAGGCC TTACCACTCT TTTGTTACTC TATTGATCCA 2341 GCTCAGCAAA GGCAGTGTGA TCTAAGATTC TATCTTCGCG ATGTAGTAAA ACTAGCTAGA 2401 CCGAGAAAGA GACTAGAAAT GCAAAAGGCA CTTCTACAAT GGCTGCCATC ATTATTATCC 2461 GATGTGACGC TGCAGCTTCT CAATGATATT CGAATACGCT TTGAGGAGAT ACAGCCTAAT 2521 ATCCGACAAA CTGTTTTACA GATTTACGAT CGTACTTGTT ACCCATCATT GAATTTTGAA 2581 CATCCGAACC TGGGAGTTTT CCCTGAAACA GATAGTATAT TTGAACCTGT ATAATAATAT 2641 ATAGTCTAGC GCTTTACGGA AGACAATGTA TGTATTTCGG TTCCTGGAGA AACTATTGCA 2701 TCTATTGCAT AGGTAATCTT GCACGTCGCA TCCCCGGTTC ATTTTCTGCG TTTCCATCTT 2761 GCACTTCAAT AGCATATCTT TGTTAACGAA GCATCTGTGC TTCATTTTGT AGAACAAAAA 2821 TGCAACGCGA GAGCGCTAAT TTTTCAAACA AAGAATCTGA GCTGCATTTT TACAGAACAG 2881 AAATGCAACG CGAAAGCGCT ATTTTACCAA CGAAGAATCT GTGCTTCATT TTTGTAAAAC 2941 AAAAATGCAA CGCGACGAGA GCGCTAATTT TTCAAACAAA GAATCTGAGC TGCATTTTTA 3001 CAGAACAGAA ATGCAACGCG AGAGCGCTAT TTTACCAACA AAGAATCTAT ACTTCTTTTT 3061 TGTTCTACAA AAATGCATCC CGAGAGCGCT ATTTTTCTAA CAAAGCATCT TAGATTACTT 3121 TTTTTCTCCT TTGTGCGCTC TATAATGCAG TCTCTTGATA ACTTTTTGCA CTGTAGGTCC 3181 GTTAAGGTTA GAAGAAGGCT ACTTTGGTGT CTATTTTCTC TTCCATAAAA AAAGCCTGAC 3241 TCCACTTCCC GCGTTTACTG ATTACTAGCG AAGCTGCGGG TGCATTTTTT CAAGATAAAG 3301 GCATCCCCGA TTATATTCTA TACCGATGTG GATTGCGCAT ACTTTGTGAA CAGAAAGTGA 3361 TAGCGTTGAT GATTCTTCAT TGGTCAGAAA ATTATGAACG GTTTCTTCTA TTTTGTCTCT 3421 ATATACTACG TATAGGAAAT GTTTACATTT TCGTATTGTT TTCGATTCAC TCTATGAATA 3481 GTTCTTACTA CAATTTTTTT GTCTAAAGAG TAATACTAGA GATAAACATA AAAAATGTAG 3541 AGGTCGAGTT TAGATGCAAG TTCAAGGAGC GAAAGGTGGA TGGGTAGGTT ATATAGGGAT 3601 ATAGCACAGA GATATATAGC AAAGAGATAC TTTTGAGCAA TGTTTGTGGA AGCGGTATTC 3661 GCAATtaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 3721 cctttcgtcg aattcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 3781 ccggagatct cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag 3841 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag 3901 attgtactga gagtgcacca taccacagct tttcaattca attcatcatt ttttttttat 3961 tctttttttt gatttcggtt tctttgaaat ttttttgatt cggtaatctc cgaacagaag 4021 gaagaacgaa ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa 4081 gaaacatgaa attgcccagt attcttaacc caactgcaca gaacaaaaac ctgcaggaaa 4141 cgaagataaa tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct 4201 gttgGTATGT ACACATTCTC CTTTTTTTTC ATCTTTTTTT CTTTATTTGC CTCTTTCCTC 4261 ACAAATCTCG AGTAGATTCG TGGTCCTCTT CATTCTTTTC TTTTTTCTTT GTCGATTACT 4321 GGGCTTTTTT TCATAGGTCT CGATTGACGC GGACGGACAA TGCGAAAAAA AAAAATTTCC 4381 AAAAGAGGAA ACGGGTTTCG AGATCTCGTG AGGATGGTTT TGGAATATTC TTCAATATTT 4441 CTGCCTGTCA TAGGATTAGG GCGAGTAATG AAACAGAATA ATACATGTAT AAATCGATCG 4501 GGAATACTAA CACTACTTTT CTTTATCTAA GCAGctgcca agctatttaa tatcatgcac 4561 gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag 4621 ttagttgaag cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact 4681 gatttttcca tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt 4741 ttactcttcg aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct 4801 gcgggtgtat acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc 4861 ccaggtattg ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc 4921 cttttgatgt tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag 4981 ggtactgttg acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga 5041 gacatgggtg gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta 5101 gatgacaagg gTgaTgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca 5161 ggatctgaca ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta 5221 gagggtgaac gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa 5281 aactaaaaaa ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca 5341 atttaattat atcagttatt accctatgcg gtgtgaaata ccgcacagat gcgtaaggag 5401 aaaataccgc atcaggaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt 5461 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca 5521 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta 5581 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta 5641 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg 5701 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga 5761 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg 5821 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcgcgccat 5881 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 5941 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 6001 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag 6061 g //