LOCUS       pRS426-ECM33_stable        6061 bp    DNA     linear       21-MAY-2019
FEATURES             Location/Qualifiers
     promoter        3938..4152
                     /label=URA3_promoter
     rep_origin      complement(428..1047)
                     /label=pBR322_origin
     gene            complement(1202..2062)
                     /dnas_title="Ampicillin"
                     /label=Ampicillin
                     /gene="Ampicillin"
     rep_origin      complement(5549..5855)
                     /label=f1_origin
     exon            4153..4204
                     /dnas_title="URA3_exon1"
                     /note=""
     exon            4535..5286
                     /dnas_title="URA3_exon2"
                     /note=""
     intron          4205..4534
                     /dnas_title="ECM33_intron (stable, WT)"
                     /note="ECM33_intron (stable, WT)"
     misc_feature    2194..3665
                     /note="2micron"
     source          1..6061
                     /dnas_title="pRS426-ECM33_stable"
ORIGIN      
        1 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
       61 gaaattgtta tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag
      121 cctggggtgc ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt
      181 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      241 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      301 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      361 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      421 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      481 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      541 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      601 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      661 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      721 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      781 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      841 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
      901 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      961 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1021 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1081 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1141 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1201 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1261 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1321 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1381 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1441 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1501 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1561 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1621 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1681 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1741 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1801 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1861 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     1921 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     1981 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2041 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2101 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2161 ttccgcgcac atttccccga aaagtgccac ctgCTTATCG ATGATAAGCT GTCAAAGATG
     2221 AGAATTAATT CCACGGACTA TAGACTATAC TAGATACTCC GTCTACTGTA CGATACACTT
     2281 CCGCTCAGGT CCTTGTCCTT TAACGAGGCC TTACCACTCT TTTGTTACTC TATTGATCCA
     2341 GCTCAGCAAA GGCAGTGTGA TCTAAGATTC TATCTTCGCG ATGTAGTAAA ACTAGCTAGA
     2401 CCGAGAAAGA GACTAGAAAT GCAAAAGGCA CTTCTACAAT GGCTGCCATC ATTATTATCC
     2461 GATGTGACGC TGCAGCTTCT CAATGATATT CGAATACGCT TTGAGGAGAT ACAGCCTAAT
     2521 ATCCGACAAA CTGTTTTACA GATTTACGAT CGTACTTGTT ACCCATCATT GAATTTTGAA
     2581 CATCCGAACC TGGGAGTTTT CCCTGAAACA GATAGTATAT TTGAACCTGT ATAATAATAT
     2641 ATAGTCTAGC GCTTTACGGA AGACAATGTA TGTATTTCGG TTCCTGGAGA AACTATTGCA
     2701 TCTATTGCAT AGGTAATCTT GCACGTCGCA TCCCCGGTTC ATTTTCTGCG TTTCCATCTT
     2761 GCACTTCAAT AGCATATCTT TGTTAACGAA GCATCTGTGC TTCATTTTGT AGAACAAAAA
     2821 TGCAACGCGA GAGCGCTAAT TTTTCAAACA AAGAATCTGA GCTGCATTTT TACAGAACAG
     2881 AAATGCAACG CGAAAGCGCT ATTTTACCAA CGAAGAATCT GTGCTTCATT TTTGTAAAAC
     2941 AAAAATGCAA CGCGACGAGA GCGCTAATTT TTCAAACAAA GAATCTGAGC TGCATTTTTA
     3001 CAGAACAGAA ATGCAACGCG AGAGCGCTAT TTTACCAACA AAGAATCTAT ACTTCTTTTT
     3061 TGTTCTACAA AAATGCATCC CGAGAGCGCT ATTTTTCTAA CAAAGCATCT TAGATTACTT
     3121 TTTTTCTCCT TTGTGCGCTC TATAATGCAG TCTCTTGATA ACTTTTTGCA CTGTAGGTCC
     3181 GTTAAGGTTA GAAGAAGGCT ACTTTGGTGT CTATTTTCTC TTCCATAAAA AAAGCCTGAC
     3241 TCCACTTCCC GCGTTTACTG ATTACTAGCG AAGCTGCGGG TGCATTTTTT CAAGATAAAG
     3301 GCATCCCCGA TTATATTCTA TACCGATGTG GATTGCGCAT ACTTTGTGAA CAGAAAGTGA
     3361 TAGCGTTGAT GATTCTTCAT TGGTCAGAAA ATTATGAACG GTTTCTTCTA TTTTGTCTCT
     3421 ATATACTACG TATAGGAAAT GTTTACATTT TCGTATTGTT TTCGATTCAC TCTATGAATA
     3481 GTTCTTACTA CAATTTTTTT GTCTAAAGAG TAATACTAGA GATAAACATA AAAAATGTAG
     3541 AGGTCGAGTT TAGATGCAAG TTCAAGGAGC GAAAGGTGGA TGGGTAGGTT ATATAGGGAT
     3601 ATAGCACAGA GATATATAGC AAAGAGATAC TTTTGAGCAA TGTTTGTGGA AGCGGTATTC
     3661 GCAATtaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     3721 cctttcgtcg aattcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     3781 ccggagatct cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     3841 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     3901 attgtactga gagtgcacca taccacagct tttcaattca attcatcatt ttttttttat
     3961 tctttttttt gatttcggtt tctttgaaat ttttttgatt cggtaatctc cgaacagaag
     4021 gaagaacgaa ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa
     4081 gaaacatgaa attgcccagt attcttaacc caactgcaca gaacaaaaac ctgcaggaaa
     4141 cgaagataaa tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct
     4201 gttgGTATGT ACACATTCTC CTTTTTTTTC ATCTTTTTTT CTTTATTTGC CTCTTTCCTC
     4261 ACAAATCTCG AGTAGATTCG TGGTCCTCTT CATTCTTTTC TTTTTTCTTT GTCGATTACT
     4321 GGGCTTTTTT TCATAGGTCT CGATTGACGC GGACGGACAA TGCGAAAAAA AAAAATTTCC
     4381 AAAAGAGGAA ACGGGTTTCG AGATCTCGTG AGGATGGTTT TGGAATATTC TTCAATATTT
     4441 CTGCCTGTCA TAGGATTAGG GCGAGTAATG AAACAGAATA ATACATGTAT AAATCGATCG
     4501 GGAATACTAA CACTACTTTT CTTTATCTAA GCAGctgcca agctatttaa tatcatgcac
     4561 gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag
     4621 ttagttgaag cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact
     4681 gatttttcca tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt
     4741 ttactcttcg aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct
     4801 gcgggtgtat acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc
     4861 ccaggtattg ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc
     4921 cttttgatgt tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag
     4981 ggtactgttg acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga
     5041 gacatgggtg gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta
     5101 gatgacaagg gTgaTgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca
     5161 ggatctgaca ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta
     5221 gagggtgaac gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa
     5281 aactaaaaaa ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca
     5341 atttaattat atcagttatt accctatgcg gtgtgaaata ccgcacagat gcgtaaggag
     5401 aaaataccgc atcaggaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt
     5461 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     5521 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     5581 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     5641 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     5701 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     5761 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     5821 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcgcgccat
     5881 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     5941 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     6001 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     6061 g
//

