LOCUS       pRS426-ECM33_unstable        6081 bp    DNA     linear       21-MAY-2019
FEATURES             Location/Qualifiers
     promoter        3938..4152
                     /label=URA3_promoter
     rep_origin      complement(428..1047)
                     /label=pBR322_origin
     gene            complement(1202..2062)
                     /dnas_title="Ampicillin"
                     /label=Ampicillin
                     /gene="Ampicillin"
     rep_origin      complement(5569..5875)
                     /label=f1_origin
     exon            4153..4204
                     /dnas_title="URA3_exon1"
                     /note=""
     exon            4555..5306
                     /dnas_title="URA3_exon2"
                     /note=""
     misc_feature    2194..3665
                     /note="2micron"
     intron          4205..4554
                     /dnas_title="ECM33_intron (unstable, Mut)"
                     /note="ECM33_intron (unstable, Mut)"
     source          1..6081
                     /dnas_title="pRS426-ECM33_unstable"
ORIGIN      
        1 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
       61 gaaattgtta tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag
      121 cctggggtgc ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt
      181 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      241 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      301 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      361 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      421 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      481 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      541 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      601 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      661 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      721 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      781 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      841 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
      901 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      961 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1021 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1081 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1141 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1201 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1261 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1321 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1381 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1441 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1501 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1561 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1621 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1681 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1741 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1801 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1861 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     1921 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     1981 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2041 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2101 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2161 ttccgcgcac atttccccga aaagtgccac ctgCTTATCG ATGATAAGCT GTCAAAGATG
     2221 AGAATTAATT CCACGGACTA TAGACTATAC TAGATACTCC GTCTACTGTA CGATACACTT
     2281 CCGCTCAGGT CCTTGTCCTT TAACGAGGCC TTACCACTCT TTTGTTACTC TATTGATCCA
     2341 GCTCAGCAAA GGCAGTGTGA TCTAAGATTC TATCTTCGCG ATGTAGTAAA ACTAGCTAGA
     2401 CCGAGAAAGA GACTAGAAAT GCAAAAGGCA CTTCTACAAT GGCTGCCATC ATTATTATCC
     2461 GATGTGACGC TGCAGCTTCT CAATGATATT CGAATACGCT TTGAGGAGAT ACAGCCTAAT
     2521 ATCCGACAAA CTGTTTTACA GATTTACGAT CGTACTTGTT ACCCATCATT GAATTTTGAA
     2581 CATCCGAACC TGGGAGTTTT CCCTGAAACA GATAGTATAT TTGAACCTGT ATAATAATAT
     2641 ATAGTCTAGC GCTTTACGGA AGACAATGTA TGTATTTCGG TTCCTGGAGA AACTATTGCA
     2701 TCTATTGCAT AGGTAATCTT GCACGTCGCA TCCCCGGTTC ATTTTCTGCG TTTCCATCTT
     2761 GCACTTCAAT AGCATATCTT TGTTAACGAA GCATCTGTGC TTCATTTTGT AGAACAAAAA
     2821 TGCAACGCGA GAGCGCTAAT TTTTCAAACA AAGAATCTGA GCTGCATTTT TACAGAACAG
     2881 AAATGCAACG CGAAAGCGCT ATTTTACCAA CGAAGAATCT GTGCTTCATT TTTGTAAAAC
     2941 AAAAATGCAA CGCGACGAGA GCGCTAATTT TTCAAACAAA GAATCTGAGC TGCATTTTTA
     3001 CAGAACAGAA ATGCAACGCG AGAGCGCTAT TTTACCAACA AAGAATCTAT ACTTCTTTTT
     3061 TGTTCTACAA AAATGCATCC CGAGAGCGCT ATTTTTCTAA CAAAGCATCT TAGATTACTT
     3121 TTTTTCTCCT TTGTGCGCTC TATAATGCAG TCTCTTGATA ACTTTTTGCA CTGTAGGTCC
     3181 GTTAAGGTTA GAAGAAGGCT ACTTTGGTGT CTATTTTCTC TTCCATAAAA AAAGCCTGAC
     3241 TCCACTTCCC GCGTTTACTG ATTACTAGCG AAGCTGCGGG TGCATTTTTT CAAGATAAAG
     3301 GCATCCCCGA TTATATTCTA TACCGATGTG GATTGCGCAT ACTTTGTGAA CAGAAAGTGA
     3361 TAGCGTTGAT GATTCTTCAT TGGTCAGAAA ATTATGAACG GTTTCTTCTA TTTTGTCTCT
     3421 ATATACTACG TATAGGAAAT GTTTACATTT TCGTATTGTT TTCGATTCAC TCTATGAATA
     3481 GTTCTTACTA CAATTTTTTT GTCTAAAGAG TAATACTAGA GATAAACATA AAAAATGTAG
     3541 AGGTCGAGTT TAGATGCAAG TTCAAGGAGC GAAAGGTGGA TGGGTAGGTT ATATAGGGAT
     3601 ATAGCACAGA GATATATAGC AAAGAGATAC TTTTGAGCAA TGTTTGTGGA AGCGGTATTC
     3661 GCAATtaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     3721 cctttcgtcg aattcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     3781 ccggagatct cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     3841 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     3901 attgtactga gagtgcacca taccacagct tttcaattca attcatcatt ttttttttat
     3961 tctttttttt gatttcggtt tctttgaaat ttttttgatt cggtaatctc cgaacagaag
     4021 gaagaacgaa ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa
     4081 gaaacatgaa attgcccagt attcttaacc caactgcaca gaacaaaaac ctgcaggaaa
     4141 cgaagataaa tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct
     4201 gttgGTATGT ACACATTCTC CTTTTTTTTC ATCTTTTTTT CTTTATTTGC CTCTTTCCTC
     4261 ACAAATCTCG AGTAGATTCG TGGTCCTCTT CATTCTTTTC TTTTTTCTTT GTCGATTACT
     4321 GGGCTTTTTT TCATAGGTCT CGATTGACGC GGACGGACAA TGCGAAAAAA AAAAATTTCC
     4381 AAAAGAGGAA ACGGGTTTCG AGATCTCGTG AGGATGGTTT TGGAATATTC TTCAATATTT
     4441 CTGCCTGTCA TACCATTAGG GCGAGTAATG AAACAGAATA ATACATGTAT AAATCGATCG
     4501 GGAATACTAA CACTACTTTT CTTTATCTAA CACTACTTTT CTTTATCTAA GCAGctgcca
     4561 agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta
     4621 ccaccaagga attactggag ttagttgaag cattaggtcc caaaatttgt ttactaaaaa
     4681 cacatgtgga tatcttgact gatttttcca tggagggcac agttaagccg ctaaaggcat
     4741 tatccgccaa gtacaatttt ttactcttcg aagacagaaa atttgctgac attggtaata
     4801 cagtcaaatt gcagtactct gcgggtgtat acagaatagc agaatgggca gacattacga
     4861 atgcacacgg tgtggtgggc ccaggtattg ttagcggttt gaagcaggcg gcagaagaag
     4921 taacaaagga acctagaggc cttttgatgt tagcagaatt gtcatgcaag ggctccctat
     4981 ctactggaga atatactaag ggtactgttg acattgcgaa gagcgacaaa gattttgtta
     5041 tcggctttat tgctcaaaga gacatgggtg gaagagatga aggttacgat tggttgatta
     5101 tgacacccgg tgtgggttta gatgacaagg gTgaTgcatt gggtcaacag tatagaaccg
     5161 tggatgatgt ggtctctaca ggatctgaca ttattattgt tggaagagga ctatttgcaa
     5221 agggaaggga tgctaaggta gagggtgaac gttacagaaa agcaggctgg gaagcatatt
     5281 tgagaagatg cggccagcaa aactaaaaaa ctgtattata agtaaatgca tgtatactaa
     5341 actcacaaat tagagcttca atttaattat atcagttatt accctatgcg gtgtgaaata
     5401 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaaacgtt aatattttgt
     5461 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     5521 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     5581 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     5641 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     5701 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     5761 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     5821 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     5881 tacagggcgc gtcgcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     5941 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     6001 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
     6061 cgtaatacga ctcactatag g
//

