LOCUS       pRS426-ACT1_unstable        6040 bp    DNA     linear       21-MAY-2019
FEATURES             Location/Qualifiers
     promoter        3938..4152
                     /label=URA3_promoter
     rep_origin      complement(428..1047)
                     /label=pBR322_origin
     gene            complement(1202..2062)
                     /dnas_title="Ampicillin"
                     /label=Ampicillin
                     /gene="Ampicillin"
     rep_origin      complement(5528..5834)
                     /label=f1_origin
     exon            4153..4204
                     /dnas_title="URA3_exon1"
                     /note=""
     exon            4514..5265
                     /dnas_title="URA3_exon2"
                     /note=""
     misc_feature    2194..3665
                     /note="2micron"
     intron          4205..4513
                     /dnas_title="ACT1_intron (unstable, WT)"
                     /note=""
     source          1..6040
                     /dnas_title="pRS426-ACT1_unstable"
ORIGIN      
        1 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
       61 gaaattgtta tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag
      121 cctggggtgc ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt
      181 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      241 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      301 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      361 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      421 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      481 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      541 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      601 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      661 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      721 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      781 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      841 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
      901 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      961 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1021 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1081 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1141 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1201 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1261 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1321 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1381 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1441 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1501 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1561 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1621 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1681 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1741 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1801 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1861 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     1921 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     1981 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2041 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2101 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2161 ttccgcgcac atttccccga aaagtgccac ctgCTTATCG ATGATAAGCT GTCAAAGATG
     2221 AGAATTAATT CCACGGACTA TAGACTATAC TAGATACTCC GTCTACTGTA CGATACACTT
     2281 CCGCTCAGGT CCTTGTCCTT TAACGAGGCC TTACCACTCT TTTGTTACTC TATTGATCCA
     2341 GCTCAGCAAA GGCAGTGTGA TCTAAGATTC TATCTTCGCG ATGTAGTAAA ACTAGCTAGA
     2401 CCGAGAAAGA GACTAGAAAT GCAAAAGGCA CTTCTACAAT GGCTGCCATC ATTATTATCC
     2461 GATGTGACGC TGCAGCTTCT CAATGATATT CGAATACGCT TTGAGGAGAT ACAGCCTAAT
     2521 ATCCGACAAA CTGTTTTACA GATTTACGAT CGTACTTGTT ACCCATCATT GAATTTTGAA
     2581 CATCCGAACC TGGGAGTTTT CCCTGAAACA GATAGTATAT TTGAACCTGT ATAATAATAT
     2641 ATAGTCTAGC GCTTTACGGA AGACAATGTA TGTATTTCGG TTCCTGGAGA AACTATTGCA
     2701 TCTATTGCAT AGGTAATCTT GCACGTCGCA TCCCCGGTTC ATTTTCTGCG TTTCCATCTT
     2761 GCACTTCAAT AGCATATCTT TGTTAACGAA GCATCTGTGC TTCATTTTGT AGAACAAAAA
     2821 TGCAACGCGA GAGCGCTAAT TTTTCAAACA AAGAATCTGA GCTGCATTTT TACAGAACAG
     2881 AAATGCAACG CGAAAGCGCT ATTTTACCAA CGAAGAATCT GTGCTTCATT TTTGTAAAAC
     2941 AAAAATGCAA CGCGACGAGA GCGCTAATTT TTCAAACAAA GAATCTGAGC TGCATTTTTA
     3001 CAGAACAGAA ATGCAACGCG AGAGCGCTAT TTTACCAACA AAGAATCTAT ACTTCTTTTT
     3061 TGTTCTACAA AAATGCATCC CGAGAGCGCT ATTTTTCTAA CAAAGCATCT TAGATTACTT
     3121 TTTTTCTCCT TTGTGCGCTC TATAATGCAG TCTCTTGATA ACTTTTTGCA CTGTAGGTCC
     3181 GTTAAGGTTA GAAGAAGGCT ACTTTGGTGT CTATTTTCTC TTCCATAAAA AAAGCCTGAC
     3241 TCCACTTCCC GCGTTTACTG ATTACTAGCG AAGCTGCGGG TGCATTTTTT CAAGATAAAG
     3301 GCATCCCCGA TTATATTCTA TACCGATGTG GATTGCGCAT ACTTTGTGAA CAGAAAGTGA
     3361 TAGCGTTGAT GATTCTTCAT TGGTCAGAAA ATTATGAACG GTTTCTTCTA TTTTGTCTCT
     3421 ATATACTACG TATAGGAAAT GTTTACATTT TCGTATTGTT TTCGATTCAC TCTATGAATA
     3481 GTTCTTACTA CAATTTTTTT GTCTAAAGAG TAATACTAGA GATAAACATA AAAAATGTAG
     3541 AGGTCGAGTT TAGATGCAAG TTCAAGGAGC GAAAGGTGGA TGGGTAGGTT ATATAGGGAT
     3601 ATAGCACAGA GATATATAGC AAAGAGATAC TTTTGAGCAA TGTTTGTGGA AGCGGTATTC
     3661 GCAATtaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     3721 cctttcgtcg aattcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     3781 ccggagatct cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     3841 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     3901 attgtactga gagtgcacca taccacagct tttcaattca attcatcatt ttttttttat
     3961 tctttttttt gatttcggtt tctttgaaat ttttttgatt cggtaatctc cgaacagaag
     4021 gaagaacgaa ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa
     4081 gaaacatgaa attgcccagt attcttaacc caactgcaca gaacaaaaac ctgcaggaaa
     4141 cgaagataaa tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct
     4201 gttgGTATGT TCTAGCGCTT GCACCATCCC ATTTAACTGT AAGAAGAATT GCACGGTCCC
     4261 AATTGCTCGA GAGATTTCTC TTTTACCTTT TTTTACTATT TTTCACTCTC CCATAACCTC
     4321 CTATATTGAC TGATCTGTAA TAACCACGAT ATTATTGGAA TAAATAGGGG CTTGAAATTT
     4381 GGAAAAAAAA AAAAAACTGA AATATTTTCG TGATAAGTGA TAGTGATATT CTTCTTTTAT
     4441 TTGCTACTGT TACTAAGTCT CATGTACTAA CATCGATTGC TTCATTCTTT TTGTTGCTAT
     4501 ATTATATGTT TAGctgccaa gctatttaat atcatgcacg aaaagcaaac aaacttgtgt
     4561 gcttcattgg atgttcgtac caccaaggaa ttactggagt tagttgaagc attaggtccc
     4621 aaaatttgtt tactaaaaac acatgtggat atcttgactg atttttccat ggagggcaca
     4681 gttaagccgc taaaggcatt atccgccaag tacaattttt tactcttcga agacagaaaa
     4741 tttgctgaca ttggtaatac agtcaaattg cagtactctg cgggtgtata cagaatagca
     4801 gaatgggcag acattacgaa tgcacacggt gtggtgggcc caggtattgt tagcggtttg
     4861 aagcaggcgg cagaagaagt aacaaaggaa cctagaggcc ttttgatgtt agcagaattg
     4921 tcatgcaagg gctccctatc tactggagaa tatactaagg gtactgttga cattgcgaag
     4981 agcgacaaag attttgttat cggctttatt gctcaaagag acatgggtgg aagagatgaa
     5041 ggttacgatt ggttgattat gacacccggt gtgggtttag atgacaaggg TgaTgcattg
     5101 ggtcaacagt atagaaccgt ggatgatgtg gtctctacag gatctgacat tattattgtt
     5161 ggaagaggac tatttgcaaa gggaagggat gctaaggtag agggtgaacg ttacagaaaa
     5221 gcaggctggg aagcatattt gagaagatgc ggccagcaaa actaaaaaac tgtattataa
     5281 gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa tttaattata tcagttatta
     5341 ccctatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     5401 gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     5461 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     5521 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     5581 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     5641 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     5701 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     5761 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     5821 gccgcgctta atgcgccgct acagggcgcg tcgcgccatt cgccattcag gctgcgcaac
     5881 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     5941 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     6001 acgacggcca gtgagcgcgc gtaatacgac tcactatagg
//

