LOCUS       MCS-Z1_SV40             1933 bp ds-DNA     circular     12-APR-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature                    234..367
                                     /label="SV40 origin of replication"
                                     /ApEinfo_revcolor=#c7b0e3
                                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature                    complement(1105..1170)
                                     /label="EM7 promoter"
                                     /ApEinfo_revcolor=#84b0dc
                                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature                    358..382
                                     /label="5' homology"
                                     /ApEinfo_revcolor=#faac61
                                     /ApEinfo_fwdcolor=#faac61
     misc_feature                    432..665
                                     /label="SV40 poly(A)"
                                     /ApEinfo_revcolor=#b1ff67
                                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature                    complement(7..23)
                                     /label="U6-gRNA-tRNA cloning site"
                                     /ApEinfo_revcolor=#d59687
                                     /ApEinfo_fwdcolor=#d59687
     misc_feature                    complement(723..1104)
                                     /label="Zeocin resistance"
                                     /ApEinfo_revcolor=#d6b295
                                     /ApEinfo_fwdcolor=#d6b295
     primer                          complement(48..68)
                                     /label="pBABE_3_primer"
                                     /ApEinfo_revcolor=#f8d3a9
                                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature                    373..430
                                     /label="MCS"
                                     /ApEinfo_revcolor=#faac61
                                     /ApEinfo_fwdcolor=#faac61
     polyA_signal                    441..632
                                     /label="SV40 late polyA"
                                     /ApEinfo_revcolor=#d59687
                                     /ApEinfo_fwdcolor=#d59687
     primer                          295..314
                                     /label="SV40pro_F_primer"
                                     /ApEinfo_revcolor=#c6c9d1
                                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature                    429..453
                                     /label="Homology 3'"
                                     /ApEinfo_revcolor=#ff9ccd
                                     /ApEinfo_fwdcolor=#ff9ccd
     Bacterial origin of replication complement(1177..1933)
                                     /label="pMB1 ori"
                                     /ApEinfo_revcolor=#b7e6d7
                                     /ApEinfo_fwdcolor=#b7e6d7
     promoter                        66..334
                                     /label="SV40_promoter"
                                     /ApEinfo_revcolor=#ffef86
                                     /ApEinfo_fwdcolor=#ffef86
     regulatory                      complement(54..269)
                                     /label="SV40_enhancer"
                                     /ApEinfo_revcolor=#b1ff67
                                     /ApEinfo_fwdcolor=#b1ff67
     transcriptional terminator      complement(671..722)
                                     /label="T0 bacterial terminator"
                                     /ApEinfo_revcolor=#f58a5e
                                     /ApEinfo_fwdcolor=#f58a5e
     origin                          233..310
                                     /label="SV40_origin"
                                     /ApEinfo_revcolor=#84b0dc
                                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 CTGCAGAAGC TTTACTAACT AGTcctgagg cggaaagaac cagctgtgga atgtgtgtca
       61 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      121 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      181 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
      241 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
      301 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
      361 tggaggccta ggGGGTCcgt acgaccggtA ctcgagGAGC TCTATAGGTA CCTCTAGATG
      421 TACACATATG GCTAGCTGGC CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC
      481 AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT
      541 TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT
      601 TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG
      661 TATGGAATTC tggactcaca aagaaaaaac gcccggtgtg caagaccgag cgttctgaac
      721 aaTTTATCCT CAGTCCTGCT CCTCTGCCAC AAAGTGCACG CAGTTGCCGG CCGGGTCGCG
      781 CAGGGCGAAC TCCCGCCCCC ACGGCTGCTC GCCGATCTCG GTCATGGCCG GCCCGGAGGC
      841 GTCCCGGAAG TTCGTGGACA CGACCTCCGA CCACTCGGCG TACAGCTCGT CCAGGCCGCG
      901 CACCCACACC CAGGCCAGGG TGTTGTCCGG CACCACCTGG TCCTGGACCG CGCTGATGAA
      961 CAGGGTCACG TCGTCCCGGA CCACACCGGC GAAGTCGTCC TCCACGAAGT CCCGGGAGAA
     1021 CCCGAGCCGG TCGGTCCAGA ACTCGACCGC TCCGGCGACG TCGCGCGCGG TGAGCACCGG
     1081 AACGGCACTG GTCAACTTGG CCATGATGGC CCTCCTATAG TGAGTCGTAT TATACTATGC
     1141 CGATATACTA TGCCGATGAT TAATTGTCAA GGATCCTAAG AACATGTGAG CAAAAGGCCA
     1201 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC
     1261 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     1321 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1381 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1441 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1501 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     1561 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     1621 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     1681 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     1741 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     1801 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     1861 TGACGCTCAG TGAAAACTCA CGTTAAGGGA TTTTGGTCAT GGCTAGTTAA TTAACATTTA
     1921 AATCAGCGGC CGC
//