LOCUS       4_S12.1_Empty_Bridge    4184 bp ds-DNA     circular     13-MAY-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(2127..2156)
                     /label="lac_promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     feature         complement(1806..1961)
                     /label="lacZ_a"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        3782..3810
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     terminator      2576..2779
                     /label="bGH_PA_terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     gene            join(3852..4184,1..528)
                     /label="Ampicillin"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        1973..1991
                     /label="T7_promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     regulatory      2248..2505
                     /label="CAG_enhancer"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     origin          3027..3333
                     /label="f1_origin"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        1358..1590
                     /label="hU6_promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     origin          683..1302
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        complement(2038..2057)
                     /label="T3_promoter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
       61 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
      121 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
      181 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
      241 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
      301 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
      361 aaatctggag ccggtgagcg tggaagccgc ggtatcattg cagcactggg gccagatggt
      421 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
      481 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
      541 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
      601 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
      661 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
      721 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      781 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      841 actgtTcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
      901 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
      961 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1021 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1081 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1141 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1201 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1261 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1321 gccttttgct ggccttttgc tcacatgtga gggcctattt cccatgattc cttcatattt
     1381 gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa
     1441 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
     1501 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
     1561 cttggcttta tatatcttGT GGAAAGGACG AAACACCggG TCTTCgaGAA GACctgtttt
     1621 agagctaGAA Atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
     1681 cgagtcggtg cTTTTTTgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
     1741 TTTTagcgcg tgcgccaatt ctgcagacaa atggCTCTaG AGACCggcgc cgctacaggg
     1801 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     1861 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     1921 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     1981 ctcactatag ggcgaattgg gtaccgggcc ccccctcgag gtcctccagc ttttgttccc
     2041 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     2101 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     2161 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     2221 accggtGGTC TCtTAGaggt acccgttaca taacttacgg taaatggccc gcctggctga
     2281 ccgcccaacg acccccgccc attgacgtca atagtaacgc caatagggac tttccattga
     2341 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     2401 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattGtgcc
     2461 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtaTG TATCCGCATC
     2521 TGATGCTACC GTGGTTGAGT TAGCGTCGga attcCTAGAG CTCGCTGATC AGCCTCGACT
     2581 GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG
     2641 GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG
     2701 AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG
     2761 GAAGAgAATA GCAGGCATGC TGGGGAgcgg ccgcaggaac ccctagtgat ggagttggcc
     2821 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     2881 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
     2941 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     3001 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3061 gcgtgaccgc tacacttgcc agcgccTtag cgcccgctcc tttcgctttc ttcccttcct
     3121 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3181 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     3241 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3301 ttaatagtgg actcttgttc caaactggaa caacactcaa Ctctatctcg ggctattctt
     3361 ttgatttata agggattttg ccgatttcgg Tctattggtt aaaaaatgag ctgatttaac
     3421 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     3481 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     3541 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     3601 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     3661 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     3721 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3781 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3841 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     3901 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     3961 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4021 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4081 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4141 agaatgactt ggttgagtac tcaccagtca cagaaaagca tctt
//