LOCUS       pSinHelper_(Gly)        6973 bp ds-DNA     circular     13-AUG-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    3297..3389
                     /label="6K"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5267..6127
                     /label="AmpR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    5095..5100
                     /label="XbaI - must maintain as a unique site in the vector. Cut here to linearize and transcribe."
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1836..2027
                     /label="E3\Gly"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        1486..1509
                     /label="26S\Promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2028..3296
                     /label="E2\Gly"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    6298..6886
                     /label="ori"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1..19
                     /label="SP6 promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3390..4778
                     /label="E1\Gly"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5162..5266
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4782..5094
                     /label="3'\UTR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 atttaggtga cactatagat tggcggcgta gtacacacta ttgaatcaaa cagccgacca
       61 attgcactac catcacaatg gagaagccag tagttaacgt agacgtagac cctcagagtc
      121 cgtttgtcgt gcaactgcaa aagagcttcc cgcaatttga ggtagtagca cagcaggtca
      181 ctccaaatga ccatgctaat gccagagcat tttcgcatct ggccagtaaa ctgatcgagc
      241 tggaggttcc taccacagcg acgattttgg acataggcag cgcaccggct cgtagaatgt
      301 tttccgagca ccagtaccat tgcgtttgcc ccatgcgtag tccagaagac ccggaccgca
      361 tgatgaaata tgccagcaaa ctggcggaaa aagcatgtaa gattacaaac aagaacttgc
      421 atgagaagat caaggacctc cggaccgtac ttgatacacc ggatgctgaa acgccatcac
      481 tctgcttcca caacgatgtt acctgcaaca cgcgtgccga gtactccgtc atgcaggacg
      541 tgtacatcaa cgctcccgga actatttacc accaggctat gaaaggcgtg cggaccctgt
      601 actggattgg cttcgacacc acccagttca tgttctcggc tatggcaggt tcgtaccctg
      661 catacaacac caactgggcc gacgaaaaag tccttgaagc gcgtaacatc ggactctgca
      721 gcacaaagct gagtgaaggc aggacaggaa agttgtcgat aatgaggaag aaggagttga
      781 agcccatcgc ctcgttcgac aaaagccaag acgacgctat ggcgttaacc ggcctgatga
      841 tcttggaaga cctgggtgtg gaccaaccac tactcgactt gatcgagtgc gcctttggag
      901 aaatatcatc cacccatctg cccacgggta cccgtttcaa attcggggcg atgatgaaat
      961 ccggaatgtt cctcacgctc tttgtcaaca cagttctgaa tgtcgttatc gccagcagag
     1021 tattggagga gcggcttaaa acgtccaaat gtgcagcatt tatcggcgac gacaacatta
     1081 tacacggagt agtatctgac aaagaaatgg ctgagaggtg tgccacctgg ctcaacatgg
     1141 aggttaagat cattgacgca gtcatcggcg agagaccacc ttacttctgc ggtggattca
     1201 tcttgcaaga ttcggttacc tccacagcgt gtcgcgtggc ggaccccttg aaaaggctgt
     1261 ttaagttggg taaaccgctc ccagccgacg atgagcaaga cgaagacaga agacgcgctc
     1321 tgctagatga aacaaaggcg tggtttagag taggtataac agacacctta gcagtggccg
     1381 tggcaactcg gtatgaggta gacaacatca cacctgtcct gctggcattg agaacttttg
     1441 cccagagcaa aagagcattt caagccatca gaggggaaat aaagcatctc tacggtggtc
     1501 ctaaatagtc agcatagtac atttcatctg actaatacca caacaccacc accatgaata
     1561 gaggattctt taacatgctc ggccgccgcc ccttcccagc ccccactgcc atgtggaggc
     1621 cgcggagaag gaggcaggcg gccccgatgc ctgcccgcaa tgggctggct tcccaaatcc
     1681 agcaactgac cacagccgtc agtgccctag tcattggaca ggcaactaga cctcaaaccc
     1741 cacgcccacg cccgccgccg cgccagaaga agcaggcggc cgccaacttt gacctactta
     1801 agttggcggg agacgttgag tccaaccctg ggccctctgc tgcaccactg gtcacggcca
     1861 tgtgcttgct tggaaacgtg agcttcccat gcaatcgccc gcccacatgc tacacccgcg
     1921 aaccatccag agctctcgac atcctcgaag agaacgtgaa ccacgaggcc tacgacaccc
     1981 tgctcaacgc catattgcgg tgcggatcgt ccggcagaag taaaagaagc gtcactgacg
     2041 actttacctt gaccagcccg tacttgggca catgctcgta ctgtcaccat actgaaccgt
     2101 gctttagccc gattaagatc gagcaggtct gggatgaagc ggacgacaac accatacgca
     2161 tacagacttc cgcccagttt ggatacgacc aaagcggagc agcaagctca aataagtacc
     2221 gctacatgtc gctcgagcag gatcatactg tcaaagaagg caccatggat gacatcaaga
     2281 tcagcacctc aggaccgtgt agaaggctta gctacaaagg atactttctc ctcgcgaagt
     2341 gtcctccagg ggacagcgta acggttagta tagcgagtag caactcagcg acgtcatgca
     2401 caatggcccg caagataaaa ccaaaattcg tgggacggga aaaatatgac ctacctcccg
     2461 ttcacggtaa gaagattcct tgcacagtgt acgaccgtct gaaagaaaca accgccggct
     2521 acatcactat gcacaggccg ggaccgcacg cctatacgtc ctatctggag gaatcatcag
     2581 ggaaagtcta cgcgaagcca ccatccggaa agaacattac gtacgagtgc aagtgcggcg
     2641 attacaagac cggtaccgtt acgacccgta ccgaaatcac gggctgcacc gccatcaagc
     2701 agtgcgtcgc ctataagagc gaccaaacga agtgggtctt caattcgccg gacttgatca
     2761 gacatgccga ccacacggcc caagggaaat tgcatttacc tttcaagctg atcccgagta
     2821 cctgcatggt ccctgttgcc cacgcgccga acgtagtaca cggctttaaa cacatcagcc
     2881 tccaattaga cacagaccac ctgacattgc tcaccaccag gagactaggg gcaaatccgg
     2941 aaccaactac tgaatggatc atcggaaaga cggttagaaa cttcaccgtc gaccgagatg
     3001 gcctggaata catatggggc aatcacgaac cggtaagggt ctatgcccaa gagtctgcac
     3061 caggagaccc tcacggatgg ccacacgaaa tagtacagca ttactaccat cgccatcctg
     3121 tgtacaccat cttagccgtc gcatcagctg ctgtggcgat gatgattggc gtaactgttg
     3181 cagcattatg tgcctgtaaa gcgcgccgtg agtgcctgac gccatatgcc ctggccccaa
     3241 atgccgtgat tccaacttcg ctggcacttt tgtgctgtgt taggtcggct aatgctgaaa
     3301 cattcaccga gaccatgagt tacctatggt cgaacagcca gccattcttc tgggtccagc
     3361 tgtgtatacc cctggccgct gtcatcgttc taatgcgctg ttgctcatgc tgcctgcctt
     3421 ttttagtggt tgccggcgcc tacctggcga aggtagacgc ctacgaacat gcgaccactg
     3481 ttccaaatgt gccacagata ccgtataagg cacttgttga aagggcaggg tacgccccgc
     3541 tcaatttgga gattactgtc atgtcctcgg aggttttgcc ttccaccaac caagagtaca
     3601 tcacctgcaa attcaccact gtggtcccct cccctaaagt caaatgctgc ggctccttgg
     3661 aatgtcagcc cgccgctcac gcagactata cctgcaaggt ctttggaggg gtgtacccct
     3721 tcatgtgggg aggagcacaa tgtttttgcg acagtgagaa cagccagatg agtgaggcgt
     3781 acgtcgaatt gtcagcagat tgcgcgactg accacgcgca ggcgattaag gtgcatactg
     3841 ccgcgatgaa agtaggacta cgtatagtgt acgggaacac taccagtttc ctagatgtgt
     3901 acgtgaacgg agtcacacca ggaacgtcta aagacctgaa agtcatagct ggaccaattt
     3961 cagcatcgtt tacaccattc gatcacaagg tcgttatcca tcgcggcctg gtgtacaact
     4021 atgacttccc ggaatacgga gcgatgaaac caggagcgtt tggagacatt caagctacct
     4081 ccttgactag caaagatctc atcgccagca cagacattag actactcaag ccttccgcca
     4141 agaacgtgca tgtcccgtac acgcaggccg catctggatt cgagatgtgg aaaaacaact
     4201 caggccgccc actgcaggaa accgcccctt tcgggtgcaa gattgcagtc aatccgcttc
     4261 gagcggtgga ctgctcatac gggaacattc ccatctctat cgacatcccg aacgctgcct
     4321 ttatcaggac atcagatgca ccactggtct caacagtcaa atgtgatgtc agtgagtgca
     4381 cttactcagc ggacttcggc gggatggcta ccctgcagta tgtatccgac cgcgaaggac
     4441 aatgccctgt acattcgcat tcgagcacag caaccctcca agagtcgaca gttcatgtcc
     4501 tggagaaagg agcggtgaca gtacacttca gcaccgcgag cccacaggcg aactttattg
     4561 tatcgctgtg tggtaagaag acaacatgca atgcagaatg caaaccacca gctgaccata
     4621 tcgtgagcac cccgcacaaa aatgaccaag aattccaagc cgccatctca aaaacttcat
     4681 ggagttggct gtttgccctt ttcggcggcg cctcgtcgct attaattata ggacttatga
     4741 tttttgcttg cagcatgatg ctgactagca cacgaagatg accgctacgc cccaatgacc
     4801 cgaccagcaa aactcgatgt acttccgagg aactgatgtg cataatgcat caggctggta
     4861 tattagatcc ccgcttaccg cgggcaatat agcaacacca aaactcgacg tatttccgag
     4921 gaagcgcagt gcataatgct gcgcagtgtt gccaaataat cactatatta accatttatt
     4981 cagcggacgc caaaactcaa tgtatttctg aggaagcatg gtgcataatg ccatgcagcg
     5041 tctgcataac tttttattat ttcttttatt aatcaacaaa attttgtttt taactctaga
     5101 gctcgagggg aattaattct tgaagacgaa agggccaggt ggcacttttc ggggaaatgt
     5161 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5221 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5281 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5341 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5401 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5461 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     5521 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5581 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5641 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5701 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5761 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5821 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5881 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5941 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6001 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6061 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6121 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6181 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6241 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6301 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6361 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6421 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6481 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6541 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6601 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6661 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6721 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6781 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6841 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6901 gagctcgtat ggacatattg tcgttagaac gcggctacaa ttaatacata accttatgta
     6961 tcatacacat acg
//