LOCUS pTSin-EGFP 10667 bp ds-DNA circular 13-AUG-2019 DEFINITION . FEATURES Location/Qualifiers misc_feature 1..18 /label="SP6 promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 5802..7635 /label="nsP4" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 8961..9821 /label="AmpR" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 215..346 /label="packaging signal" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 4119..5801 /label="nsP3" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 1698..4118 /label="nsP2" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 8856..8960 /label="AmpR Promoter" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 7706..8425 /label="EGFP" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 8789..8794 /label="XbaI - must maintain as a unique site in the vector. Cut here to linearize and transcribe." /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 19..77 /label="5'\UTR" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 7612..7635 /label="26S\Promoter" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 78..1697 /label="nsP1" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 8443..8756 /label="3'\UTR" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 9992..10580 /label="ori" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc ORIGIN 1 ATTTAGGTGA cactatagAt tgGcggcgta gtaCacacta ttgaatcaaa cagccgacca 61 attgcactac catcacaatg gagaagccag tagttaacgt agacgtagac ccgcagagtc 121 cgtttgtcgt gcaactgcaa aagagcttcc cgcaatttga ggtagtagca cagcaggtca 181 ctccaaatga ccatgctaat gccagagcat tttcgcatct ggccagtaaa ctaatcgagc 241 tggaggttcc taccacagcg acgattttgg acataggcag cgcaccggct cgtagaatgt 301 tttccgagca ccagtaccat tgcgtttgcc ccatgcgtag tccagaagac ccggaccgca 361 tgatgaaata tgccagcaaa ctggcggaaa aagcatgcaa gattacgaat aagaacttgc 421 atgagaagat caaggacctc cggaccgtac ttgatacacc ggatgctgaa acgccatcac 481 tctgcttcca caacgatgtt acctgcaaca cgcgtgccga gtactccgtc atgcaggacg 541 tgtacatcaa cgcacccgga actatttacc atcaggctat gaaaggcgtg cggaccctgt 601 actggattgg cttcgatacc acccagttca tgttctcggc tatggcaggt tcgtaccctg 661 cgtacaacac caactgggcc gacgaaaaag tcctcgaagc gcgtaacatc ggactctgca 721 gcacaaagct gagtgaaggc aggacaggaa agttgtcgat aatgaggaag aaggagttga 781 agcccgggtc acgggtttat ttctccgttg gatcgacact ttacccagaa cacagagcca 841 gcttgcagag ctggcatctt ccatcggtgt tccacctgaa aggaaagcag tcgtacactt 901 gccgctgtga tacagtggtg agctgcgaag gctacgtagt gaagaaaatc accatcagtc 961 ccgggatcac gggagaaacc gtgggatacg cggttacaaa caatagcgag ggcttcttgc 1021 tatgcaaagt taccgataca gtaaaaggag aacgggtatc gttccccgtg tgcacgtata 1081 tcccggccac catatgcgat cagatgaccg gcataatggc cacggatatc tcacctgacg 1141 atgcacaaaa acttctggtt gggctcaacc agcgaatcgt cattaacggt aagactaaca 1201 ggaacaccaa taccatgcaa aattaccttc tgccaatcat tgcacaaggg ttcagcaaat 1261 gggccaagga gcgcaaagaa gaccttgaca atgaaaaaat gctgggtacc agagagcgca 1321 agcttacata tggctgcttg tgggcgtttc gcactaagaa agtgcactcg ttctatcgcc 1381 cacctggaac gcagaccatc gtaaaagtcc cagcctcttt tagcgctttc cccatgtcat 1441 ccgtatggac tacctctttg cccatgtcgc tgaggcagaa gataaaattg gcattacaac 1501 caaagaagga ggaaaaactg ctgcaagtcc cggaggaatt agtcatggag gccaaggctg 1561 ctttcgagga tgctcaggag gaatccagag cggagaagct ccgagaagca ctcccaccat 1621 tagtggcaga caaaggtatc gaggcagccg cggaagttgt ctgcgaagtg gaggggctcc 1681 aggcggacat cggagcagca ctcgtcgaaa ccccgcgcgg tcatgtaagg ataatacctc 1741 aagcaaatga ccgtatgatc ggacagtaca tcgttgtctc gccaacctct gtgctgaaga 1801 acgctaaact cgcaccagca cacccgctag cagaccaggt taagatcata acgcactccg 1861 gaagatcagg aaggtatgca gtcgaaccat acgacgctaa agtactgatg ccagcaggaa 1921 gtgccgtacc atggccagaa ttcttagcac tgagtgagag cgccacgcta gtgtacaacg 1981 aaagagagtt tgtgaaccgc aagctgtacc atattgccat gcacggtccc gctaagaata 2041 cagaagagga gcagtacaag gttacaaagg cagagctcgc agaaacagag tacgtgtttg 2101 acgtggacaa gaagcgatgc gtcaagaagg aagaagcctc aggacttgtc ctctcgggag 2161 aactgaccaa cccgccctat cacgaactag ctcttgaggg actgaagact cgacccgcgg 2221 tcccgtacaa ggttgaaaca ataggagtga taggcAcacc aggatcgggc aagtcggcta 2281 tcatcaagtc aactgtcacg gcacgtgatc ttgttaccag cggaaagaaa gaaaactgcc 2341 gcgaaattGa ggccgatgtg ctacggctga ggggcatgca gatcacgtcg aagacagtgg 2401 attcggttat gctcaacgga tgccacaaag ccgtagaagt gctgtatgtt gacgaagcgt 2461 tcgcgtgcca cgcaggagca ctacttgcct tgattgcaat cgtcagaccc cgtaagaagg 2521 tagtgctatg cggagaccct aagcaatgcg gattcttcaa catgatgcaa ctaaaggtat 2581 atttcaacca cccggaaaaa gacatatgta ccaagacatt ctacaagttt atctcccgac 2641 gttgcacaca gccagtcacg gctattgtat cgacactgca ttacgatgga aaaatgaaaa 2701 ccacaaaccc gtgcaagaag aacatcgaaa tcgacattac aggggccacg aagccgaagc 2761 caggggacat catcctgaca tgcttccgcg ggtgggttaa gcaactgcaa atcgactatc 2821 ccggacatga ggtaatgaca gccgcggcct cacaagggct aaccagaaaa ggagtatatg 2881 ccgtccggca aaaagtcaat gaaaacccgc tgtacgcgat cacatcagag catgtgaacg 2941 tgctgctcac ccgcactgag gacaggctag tatggaaaac tttacagggc gacccatgga 3001 ttaagcagct cactaacgta ccaaaaggaa attttcaagc caccatcgag gactgggaag 3061 ctgaacacaa gggaataatt gctgcgataa acagtcccgc tccccgtacc aatccgttca 3121 gctgcaagac taacgtttgc tgggcgaaaG Cactggaacc gatactggcc acggccggta 3181 tcgtacttac cggttgccag tggagcgagc tgttcccaca gtttgcagat gacaaaccac 3241 actcggccat ctacgccctg gacgtaatct gcattaagtt tttcggcatg gacttgacaa 3301 gcggactgtt ttccaaacag agcatcccgt taacgtacca tcctgccgat tcagcgaggc 3361 cagtagctca ttgggacaac agcccaggaa cccgcaagta tgggtacgat cacgccgttg 3421 ccgccgaact ctcccgtaga tttccggtgt tccagctagc tgggaaaggc acacagcttg 3481 atttgcagac gggcagaact agagttatct ccgcacagca taacttggtc ccagtgaacc 3541 gcaatctccc gcacgcctta gtccccgagc acaaggagaa acaacccggc ccggtcaaaa 3601 aattcttgag ccagttcaaa caccactccg tacttgtggt ctcagaggaa aaaattgaag 3661 ctccccacaa gagaatcgaa tggatcgccc cgattggcat agccggcgct gataagaact 3721 acaacctggc tttcgggttt ccgccgcagg cacggtacga cctggtgttt atcaatattg 3781 gaactaaata cagaaaccat cactttcagc agtgcgaaga ccatgcggcg accttgaaaa 3841 ccctctcgcg ttcggccctg aactgcctta accccggagg caccctcgtg gtgaagtcct 3901 acggttacgc cgaccgcaat agtgaggacg tagtcaccgc tcttgccaga aaatttgtca 3961 gagtgtctgc agcgaggcca gagtgcgtct caagcaatac agaaatgtac ctgatcttcc 4021 gacaactaga caacagccgc acacgacaat tcaccccgca tcatctgaat tgtgtgattt 4081 cgtccgtgta cgagggtaca agagacggag ttggagccgc accgtcatac cgcactaaaa 4141 gggagaacat tgctgattgt caagaggaag cagttgtcaa tgcagccaat ccgctgggca 4201 gaccaggcga aggagtctgc cgtgccatct ataaacgttg gccgaacagt ttcaccgatt 4261 cagccacaga gaccggcacc gcaaaactga ctgtgtgcca aggaaagaaa gtgatccacg 4321 cggttggccc tgatttccgg aaacacccag aggcagaagc cctgaaattg ctgcaaaacg 4381 cctaccatgc agtggcagac ttagtaaatg aacataatat caagtctgtc gccatcccac 4441 tgctatctac aggcatttac gcagccggaa aagaccgcct tgaagtatca cttaactgct 4501 tgacaaccgc gctagataga actgatgcgg acgtaaccat ctactgcctg gataagaagt 4561 ggaaggaaag aatcgacgcg gtgctccaac ttaaggagtc tgtaacagag ctgaaggatg 4621 aggatatgga gatcgacgac gagttagtat ggatccatcc ggacagttgc ctgaagggaa 4681 gaaagggatt cagtactaca aaaggaaagt tgtattcgta ctttgaaggc accaaattcc 4741 atcaagcagc aaaagatatg gcggagataa aggtcctgtt cccaaatgac caggaaagCa 4801 acgagcaact gtgtgcctac atattggggg agaccatgga agcaatccgc gaaaaatgcc 4861 cggtcgacca caacccgtcg tctagcccgc caaaaacgct gccgtgcctc tgCatgtatg 4921 ccatgacgcc agaaagggtc cacagactca gaagcaacaa cgtcaaagaa gttacagtat 4981 gctcctccac cccccttcca aagtacaaaa tcaagaacgt tcagaaggtt cagtgcacaa 5041 aagtagtcct gtttaacccg catacccctg cattcgttcc cgcccgtaag tacatagaag 5101 cgccagaaca gcctgcagct ccgcctgcac aggccgagga ggcccccgaa gttgcagcaa 5161 caccaacacc acctgcagct gataacacct cgcttgatgt cacggacatc tcactggaca 5221 tggaagacag tagcgaaggc tcactctttt cgagctttag cggatcggac aactctatta 5281 ctagtatgga cagttggtcg tcaggaccta gttcactaga gatagtagac cgaaggcagg 5341 tggtggtggc tgacgtccat gccgtccaag agcctgcccc tgttccaccg ccaaggctaa 5401 agaagatggc ccgcctggca gcggcaagaa tgcaggaaga gccaactcca ccggcaagca 5461 ccagctctgc ggacgagtcc cttcaccttt cttttggtgg ggtatccatg tccttcggat 5521 cccttttcga cggagagatg gcccgcttgg cagcggcaca acccccggca agtacatgcc 5581 ctacggatgt gcctatgtct ttcggatcgt tttccgacgg agagattgag gagctgagcc 5641 gcagagtaac cgagtctgag cccgtcctgt ttgggtcatt tgaaccgggc gaagtgaact 5701 caattatatc gtcccgatca gccgtatctt ttccaccacg caagcagaga cgtagacgca 5761 ggagcaggag gaccgaatac tgactaaccg gggtaggtgg gtacatattt tcgacggaca 5821 caggccctgg gcacttgcaa aagaagtccg ttctgcagaa ccagcttaca gaaccgacct 5881 tggagcgcaa tgttctggaa agaatctacg ccccggtgct cgacacgtcg aaagaggaac 5941 agctcaaact caggtaccag atgatgccca ccgaagccaa caaaagcagg taccagtcca 6001 gaaaagtaga aaatcagaaa gccataacca ctgagcgact gctttcaggg ctacgactgt 6061 ataactctgc cacagatcag ccagaatgct ataagatcac ctacccgaaa ccatcgtatt 6121 ccagcagtgt accggcgaac tactctgacc caaagtttgc tgtagctgtt tgcaacaact 6181 atctgcatga gaattacccg acggtagcat cttatcagat caccgacgag tacgatgctt 6241 acttggatat ggtagacggg acagtcgctt gcctagatac tgcaactttt tgccccgcca 6301 agcttagaag ttacccgaaa agacacgagt atagagcccc aaacatccgc agtgcggttc 6361 catcagcgat gcagaacacg ttgcaaaacg tgctcattgc cgcgactaaa agaaactgca 6421 acgtcacaca aatgcgtgaa ttgccaacac tggactcagc gacattcaac gttgaatgct 6481 ttcgaaaata tgcatgtaat gacgagtatt gggaggagtt tgcccgaaag ccaattagga 6541 tcactactga gttcgttacc gcatacgtgg ccagactgaa aggccctaag gccgccgcac 6601 tgttcgcaaa gacgcataat ttggtcccat tgcaagaagt gcctatggat aggttcgtca 6661 tggacatgaa aagagacgtg aaagttacac ctggcacgaa acacacagaa gaaagaccga 6721 aagtacaagt gatacaagcc gcagaacccc tggcgaccgc ttacctgtgc gggatccacc 6781 gggagttagt gcgcaggctt acagccgtct tgctacccaa cattcacacg ctttttgaca 6841 tgtcggcgga ggactttgat gcaatcatag cagaacactt caagcaaggt gacccggtac 6901 tggagacgga tatcgcctcg ttcgacaaaa gccaagacga cgctatggcg ttaactggcc 6961 tgatgatctt ggaagacctg ggtgtggacc aaccactact cgacttgatc gagtgcgcct 7021 ttggagaaat atcatccacc catctgccca cgggtacccg tttcaaattc ggggcgatga 7081 tgaaatccgg aatgttcctc acgctctttg tcaacacagt tctgaatgtc gttatcgcca 7141 gcagagtatt ggaggagcgg cttaaaacgt ccaaatgtgc agcatttatc ggcgacgaca 7201 acatcataca cggagtagta tctgacaaag aaatggctga gaggtgtgcc acctggctca 7261 acatggaggt taagatcatt gacgcagtca tcggcgagag accgccttac ttctgcggtg 7321 gattcatctt gcaagattcg gttacctcca cagcgtgtcg cgtggcggac cccttgaaaa 7381 ggctgtttaa gttgggtaaa ccgctcccag ccgacgatga gcaagacgaa gacagaagac 7441 gcgctctgct agatgaaaca aaggcgtggt ttagagtagg tataacagac accttagcag 7501 tggccgtggc aactcggtat gaggtagaca acatcacacc tgtcctgctg gcattgagaa 7561 cttttgccca gagcaaaaga gcatttcaag ccatcagagg ggaaataaag catctctacg 7621 gtggtcctaa atagtcagca tagtacattt catctgacta ataccacaac accaccagcg 7681 gccgcatcga tcaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt 7741 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 7801 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 7861 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt 7921 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 7981 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 8041 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 8101 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 8161 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 8221 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 8281 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc 8341 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 8401 cggcatggac gagctgtaca agtaaagcgg catcgatact agtacgcccc aatgacccga 8461 ccagcaaaac tcgatgtact tccgaggaac tgatgtgcat aatgcatcag gctggtatat 8521 tagatccccg cttaccgcgg gcaatatagc aacaccaaaa ctcgacgtat ttccgaggag 8581 gcgcagtgca taatgctgcg cagtgttgcc aaataatcac tatattaacc atttattcag 8641 cggacgccaa aactcaatgt atttctgagg aagcatggtg cataatgcca tgcagcgtct 8701 gcataacttt ttattatttc ttttattaat caacaaaatt ttgtttttaa catttcaaaa 8761 aaaaaaaaaa aaaaAGGGGA TCTCGAGCTC TAGAGCTCga ggggaattaa ttcttgaaga 8821 cgaaagggcc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 8881 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 8941 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 9001 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 9061 ctgaagatca gttgggtgca cgAgtgggtt acatcgaact ggatctcaac agcggtaaga 9121 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 9181 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 9241 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 9301 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 9361 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 9421 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 9481 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 9541 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 9601 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 9661 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 9721 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 9781 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 9841 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 9901 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 9961 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 10021 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 10081 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 10141 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 10201 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 10261 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 10321 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 10381 agcTAtgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 10441 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 10501 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 10561 gggggcggag cctatggaaa aacgccagca acgcgagctc GTATGGACAT ATTGTCGTTA 10621 GAACGCGGCT ACAATTAATA CATAACCTTA TGTATCATAC ACATACG //