LOCUS       Exported                5273 bp ds-DNA     circular SYN 21-MAY-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Weatherall Institute
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2019 from SnapGene 4.2.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..154
                     /label=Upstream polyA / transcriptional pause
                     /note="Upstream polyA / transcriptional pause"
     misc_feature    162..179
                     /label=MCS
                     /note="MCS"
     enhancer        180..963
                     /label=retE1.1
     misc_feature    972..993
                     /label=MCS
                     /note="MCS"
     misc_feature    1011..1266
                     /label=HSV-tk basal promoter
                     /note="HSV-tk basal promoter"
     misc_feature    1317..1449
                     /label=chimeric intron
                     /note="chimeric intron"
     misc_feature    1509..2218
                     /label=Citrine
                     /note="Citrine"
     misc_feature    2219..2228
                     /label=GFP
                     /note="GFP"
     misc_feature    2229..2255
                     /label=Untitled
     misc_feature    2251..2350
                     /label=Codeset probe_045
                     /note="Codeset probe_045"
     misc_feature    2256..2345
                     /label=Nanotag_045
                     /note="Nanotag_045"
     misc_feature    2346..2357
                     /label=Untitled
     misc_feature    2388..2609
                     /label=SV40 late polyA region
                     /note="SV40 late polyA region"
     misc_feature    complement(3699..4556)
                     /label=Beta-lactamase AmpR gene
                     /note="Beta-lactamase AmpR gene"
     misc_feature    4689..5143
                     /label=f1 origin
                     /note="f1 origin"
ORIGIN
        1 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
       61 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
      121 cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat aggtaccgag ctcgagttga
      181 tgaaggatca cgggacagtg atgtcatcat tttaagactt gctatgtcta agtgcactgc
      241 caggaagaga aaagatgaat gatgatttat gtgccttgga tagaattcag gtaaaaacct
      301 cttcaaaaat gttaattatt tgaggtcact ttctattaca taggatctgt tctttagaat
      361 ttctcacagg ttaatagaga tgattcagga ggtctcagcc tggattggtc tgacaagaaa
      421 aagcagcaac tgtgtggggg agggaaaagg aggagacaaa gggtgtgatt tatcgatgtg
      481 cactttgcta tttgtgacta acaaagagat ggttcacaaa tgtctcaggc tcaaagctgt
      541 gatctgtcag aactgctgag ctggggaaat actaccacta agtgaaaata gattggtcag
      601 cagttagaga cttatctgca aggaggtcat cgcgtactat ctgtacgtgc agcaggaaga
      661 tcgtttggct gggttaggag tgagataact ttgttgcagt gttgtctgtt atcaatcaat
      721 agttttaatt agttacagga ttaactaata aaaacccttg ctgattaatt gatttttttt
      781 ttttttttaa atctgggtaa cgcttgcttt attacggagg tgatttttca tttgaactaa
      841 atgctttagc ccaaatgcag agggttttta ttgtttctga gggaatgaaa gaatactgag
      901 gcgtgtagga gaaggaacac tgatgtctgt caggaaagca aatatcaagg atactggtga
      961 tcagtgttcg cggctagccc gggctcgaga tctgcgatct aagtaagctt gctgcttcat
     1021 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
     1081 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
     1141 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt
     1201 ggcctcgaac accgagcgac cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca
     1261 gatcccaagc ttgcggccgc ttaagctgca gaagttggtc gtgaggcact gggcaggtaa
     1321 gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga
     1381 gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc
     1441 tctccacagg tgtccactcc caggttcaat acagctctta agcggccgca agctagcttg
     1501 ccgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1561 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1621 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1681 ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc taccccgacc
     1741 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1801 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1861 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1921 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1981 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2041 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2101 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2161 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2221 acaagtgagc ctctagactc cctcagcggc gcgcctggtg ttcctcgaga tcggagtcct
     2281 aaatcgactg ccttagcggt aggacagtag atcagtcagc gacgtgacga accctcgcag
     2341 gatcaagatc tcacgtgtct agagtcgggg cggccggccg cttcgagcag acatgataag
     2401 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     2461 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     2521 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     2581 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct
     2641 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
     2701 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc
     2761 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2821 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2881 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2941 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3001 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3061 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3121 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3181 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3241 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3301 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3361 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3421 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3481 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3541 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3601 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3661 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     3721 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     3781 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     3841 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     3901 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     3961 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4021 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4081 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4141 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4201 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4261 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4321 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4381 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4441 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4501 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4561 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4621 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     4681 ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4741 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4801 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4861 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4921 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4981 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5041 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5101 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttgccattc gccattcagg
     5161 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagcccaag
     5221 ctaccatgat aagtaagtaa tattaaggta cgggaggtac ttggagcggc cgc
//
