LOCUS       #141_pH7_Ubi10-RcCHAD-mCherry     12564 bp    DNA     circular UNA 19-JUL-2017
DEFINITION  attL4-attR1
ACCESSION   UB10-promoteur/pDONRP4-P1R
FEATURES             Location/Qualifiers
     misc_feature    complement(join(12558..12564,1..>7))
                     /label="attL1"
     misc_recomb     complement(join(12558..12564,1..14))
                     /label="attB1"
     misc_feature    complement(7..>7)
                     /feature_type="Insertion"
                     /note="Region on donor sequence (pDONR221): 2898..652"
                     /label="pDONR221"
     unsure          6^7
                     /Design_Description="Generate PCR product using computed
                     primers and a DNA polymerase (i.e. Platinum Pfx or
                     Platinum Taq).	Check quality and purify product by
                     PEG/Mg2+ precipitation and agarose gels.	Mix purified PCR
                     product and pDONR Vector pDONRP4-P1R in BP buffer and TE
                     buffers.	Thaw, vortex and add BP Clonase enzyme. Incubate
                     >= 1 hr. at 25�.	Add Proteinase K for 10 min at 37�.
                     Transform into DH5a or TOP10 cells and plate on kanamycin
                     plates.	Pick colonies and grow in selection media."
                     /label="Gateway Cloning Description"
     misc_feature    complement(<8..14)
                     /label="attR1"
     misc_feature    complement(<8..15)
                     /feature_type="Insertion"
     5'UTR           complement(16..515)
                     /note="Oriinal location: 1520..2019"
                     /label="5'UTR"
     intron          complement(16..319)
                     /note="Oriinal location: 1716..2019"
                     /label="Intron 1.1"
     misc_feature    complement(16..2001)
                     /feature_type="Insertion"
                     /note="Region on donor sequence (pNIGEL 16
                     (pGII(A)-KAN-UBQ10:3H-CER_HOST)): 34..2019"
                     /label="pNIGEL 16 (pGII(A)-KAN-UBQ10:3H-CER_HOST)"
     misc_feature    complement(320..404)
                     /note="Oriinal location: 1631..1715"
                     /label="Exon 1.1"
     promoter        complement(405..2001)
                     /note="Oriinal location: 34..1630"
                     /label="5' Promoter region"
     misc_feature    complement(2002..2016)
                     /feature_type="Insertion"
     misc_feature    complement(2004..>2017)
                     /label="attL4"
     misc_recomb     complement(2004..2024)
                     /label="attB4"
     misc_feature    join(<2011..2085,10585..>10899)
                     /feature_type="Insertion"
                     /note="Region on donor sequence (pKm34GW7,1): 1..2092"
                     /label="pKm34GW7,1"
     misc_feature    complement(2017..>2017)
                     /feature_type="Insertion"
                     /note="Region on donor sequence (pDONRP4-P1R (p5')):
                     2831..675"
                     /label="pDONRP4-P1R (p5')"
     misc_feature    2086..10584
                     /feature_type="Insertion"
                     /note="Region on donor sequence (pH+,1): 11..8503"
                     /label="pH+,1"
     misc_signal     2088..2287
                     /note="Oriinal location: 2095..2294"
                     /label="RB"
     misc_feature    complement(7191..8440)
                     /note="Oriinal location: complement(7198..8447)"
                     /label="Sm/SpR"
     misc_signal     8446..8778
                     /note="Oriinal location: 8453..8785"
                     /label="LB"
     misc_feature    complement(8782..10574)
                     /note="Oriinal location: complement(8789..10575)"
                     /label="Hyg"
     terminator      10605..10830
                     /note="Oriinal location: 21..246"
                     /label="T35S"
     misc_recomb     10893..10913
                     /label="attB3"
     misc_feature    <10900..10913
                     /label="attL3"
     unsure          10905^10906
                     /Design_Description="If necessary, linearize attB
                     sequence attB2-mCherry-attB3 at a unique REN site outside
                     the attB region. Ethanol precipitate and resuspend.	Mix
                     linearized attB sequence attB2-mCherry-attB3 and pDONR
                     Vector pDONRP2R-P3 (p3') in BP buffer and TE buffers.
                     Thaw, vortex and add BP Clonase enzyme. Incubate >= 1 hr.
                     at 25�.	Add Proteinase K for 10 min at 37�.	Transform
                     into DH5a or TOP10 E. coli or any non-F' episome bearing
                     strain.	Select Entry Clones on agar plates with
                     antibiotic kanamycin."
                     /label="Gateway Cloning Description"
     CDS             complement(10914..11622)
                     /label="mCherry+STOP"
     misc_feature    11625..>11631
                     /label="attR2"
     misc_recomb     11625..11645
                     /label="attB2"
     misc_feature    <11632..11645
                     /label="attL2"
     unsure          11637^11638
                     /Design_Description="If necessary, linearize attB
                     sequence CDC26 at a unique REN site outside the attB
                     region. Ethanol precipitate and resuspend.	Mix linearized
                     attB sequence CDC26 and pDONR Vector pDONR221 in BP
                     buffer and TE buffers.	Thaw, vortex and add BP Clonase
                     enzyme. Incubate >= 1 hr. at 25�.	Add Proteinase K for 10
                     min at 37�.	pDONR Vector and attB sequence carry
                     identical resistance markers or pDONR vector is not
                     adequately annotated.	Check annotation and if necessary
                     use a different vector or construct the Entry Clone from
                     a pDONR with different resistance."
                     /label="Gateway Cloning Description"
     CDS             complement(11650..12552)
                     /label="RcCHAD"
ORIGIN
        1 TTTGTACAAA CTTGCCTGTT AATCAGAAAA ACTCAGATTA ATCGACAAAT TCGATCGCAC
       61 AAACTAGAAA CTAACACCAG ATCTAGATAG AAATCACAAA TCGAAGAGTA ATTATTCGAC
      121 AAAACTCAAA TTATTTGAAC AAATCGGATG ATATCTATGA AACCCTAATC GAGAATTAAG
      181 ATGATATCTA ACGATCAAAC CCAGAAAATC GTCTTCGATC TAAGATTAAC AGAATCTAAA
      241 CCAAAGAACA TATACGAAAT TGGGATCGAA CGAAAACAAA ATCGAAGATT TTGAGAGAAT
      301 AAGGAACACA GAAATTTACC TTGATCACGG TAGAGAGAAT TGAGAGAAAG TTTTTAAGAT
      361 TTTGAGAAAT TGAAATCTGA ATTGTGAAGA AGAAGAGCTC TTTGGGTATT GTTTTATAGA
      421 AGAAGAAGAA GAAAAGACGA GGACGACTAG GTCACGAGAA AGCTAAGGCG GTGAAGCAAT
      481 AGCTAATAAT AAAATGACAC GTGTATTGAG CGTTGTTTAC ACGCAAAGTT GTTTTTGGCT
      541 AATTGCCTTA TTTTTAGGTT GAGGAAAAGT ATTTGTGCTT TGAGTTGATA AACACGACTC
      601 GTGTGTGCCG GCTGCAACCA CTTTGACGCC GTTTATTACT GACTCGTCGA CAACCACAAT
      661 TTCTAACGGT CGTCATAAGA TCCAGCCGTT GAGATTTAAC GATCGTTACG ATTTATATTT
      721 TTTTAGCATT ATCGTTTTAT TTTTTAAATA TACGGTGGAG CTGAAAATTG GCAATAATTG
      781 AACCGTGGGT CCCACTGCAT TGAAGCGTAT TTCGTATTTT CTAGAATTCT TCGTGCTTTA
      841 TTTCTTTTCC TTTTTGTTTT TTTTTGCCAT TTATCTAATG CAAGTGGGCT TATAAAATCA
      901 GTGAATTTCT TGGAAAAGTA ACTTCTTTAT CGTATAACAT ATTGTGAAAT TATCCATTTC
      961 TTTTAATTTT TTAGTGTTAT TGGATATTTT TGTATGATTA TTGATTTGCA TAGGATAATG
     1021 ACTTTTGTAT CAAGTTGGTG AACAAGTCTC GTTAAAAAAG GCAAGTGGTT TGGTGACTCG
     1081 ATTTATTCTT GTTATTTAAT TCATATATCA ATGGATCTTA TTTGGGGCCT GGTCCATATT
     1141 TAACACTCGT GTTCAGTCCA ATGACCAATA ATATTTTTTC ATTAATAACA ATGTAACAAG
     1201 AATGATACAC AAAACATTCT TTGAATAAGT TCGCTATGAA GAAGGGAACT TATCCGGTCC
     1261 TAGATCATCA GTTCATACAA ACCTCCATAG AGTTCAACAT CTTAAACAAG AATATCCTGA
     1321 TCTGAAGAAT GTGGAGGCTT TAGTCCCTTG GATACTTGGG AGGCTGTGGA AGAACAGAAA
     1381 CGAGCTGGTG CTTAAAGGGA GGGAATTTGG AACCAATGAG GTATTAGTAA GGACACAAGA
     1441 AGATGCAGAT GAGTGGATTA GAAGGAAAGA GGCTCAGAAT GTAAGGAAAG CACCAACCAC
     1501 AACACCGTCG GGACAGACGA GAAACATGGG AAGCTCCACC ACAATCTTGG GTTAAGTGCA
     1561 ACTTTGATGG GGCATGGCCA ACAGAAGGAT TAAAATGTGG CTTAGGGTGG GTGCTTCGCG
     1621 ATCATACAGG GAAGGTGTTA TGGTTAGGTG CAAGAGCTGT GGTAAAAGTA AGAAGCGTGC
     1681 TGGAAGTAGA AGTGGAGGCT CTTAGATGGG CTGTGCTGTC ATTATCCCGA TTCAATTATA
     1741 GGAAGATCAT TTTTGAGGTG GATTCTCAGC AACTTGTGTC TTTGGTTACA TGAAAGTTAT
     1801 GCTTGTCAAG TCTCAATCCA ATTATCCAAG ACATAAAGTA TCTACTTAGC AAGTTTGAGG
     1861 ATTTTATGCT TGTGCATACA AGCCGAGAAG GAAATGGAGT GGCAGATAGA ATAGCTAAGG
     1921 AATCTCTTTC TTTTGAGAAT TATGATCTAA AGTTGTATTC TATTGTACCA ATTTGGGTTA
     1981 AAAGCTCTGT TGAGCTAGAC TAGCAACTTT TCTATACAAA GTTGATAGCT TGGCGTAATC
     2041 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC GGGCCCAAGC TTAGCTTGAG
     2101 CTTGGATCAG ATTGTCGTTT CCCGCCTTCA GTTTAAACTA TCAGTGTTTG ACAGGATATA
     2161 TTGGCGGGTA AACCTAAGAG AAAAGAGCGT TTATTAGAAT AACGGATATT TAAAAGGGCG
     2221 TGAAAAGGTT TATCCGTTCG TCCATTTGTA TGTGCATGCC AACCACAGGG TTCCCCTCGG
     2281 GATCAAAGTA CTTTGATCCA ACCCCTCCGC TGCTATAGTG CAGTCGGCTT CTGACGTTCA
     2341 GTGCAGCCGT CTTCTGAAAA CGACATGTCG CACAAGTCCT AAGTTACGCG ACAGGCTGCC
     2401 GCCCTGCCCT TTTCCTGGCG TTTTCTTGTC GCGTGTTTTA GTCGCATAAA GTAGAATACT
     2461 TGCGACTAGA ACCGGAGACA TTACGCCATG AACAAGAGCG CCGCCGCTGG CCTGCTGGGC
     2521 TATGCCCGCG TCAGCACCGA CGACCAGGAC TTGACCAACC AACGGGCCGA ACTGCACGCG
     2581 GCCGGCTGCA CCAAGCTGTT TTCCGAGAAG ATCACCGGCA CCAGGCGCGA CCGCCCGGAG
     2641 CTGGCCAGGA TGCTTGACCA CCTACGCCCT GGCGACGTTG TGACAGTGAC CAGGCTAGAC
     2701 CGCCTGGCCC GCAGCACCCG CGACCTACTG GACATTGCCG AGCGCATCCA GGAGGCCGGC
     2761 GCGGGCCTGC GTAGCCTGGC AGAGCCGTGG GCCGACACCA CCACGCCGGC CGGCCGCATG
     2821 GTGTTGACCG TGTTCGCCGG CATTGCCGAG TTCGAGCGTT CCCTAATCAT CGACCGCACC
     2881 CGGAGCGGGC GCGAGGCCGC CAAGGCCCGA GGCGTGAAGT TTGGCCCCCG CCCTACCCTC
     2941 ACCCCGGCAC AGATCGCGCA CGCCCGCGAG CTGATCGACC AGGAAGGCCG CACCGTGAAA
     3001 GAGGCGGCTG CACTGCTTGG CGTGCATCGC TCGACCCTGT ACCGCGCACT TGAGCGCAGC
     3061 GAGGAAGTGA CGCCCACCGA GGCCAGGCGG CGCGGTGCCT TCCGTGAGGA CGCATTGACC
     3121 GAGGCCGACG CCCTGGCGGC CGCCGAGAAT GAACGCCAAG AGGAACAAGC ATGAAACCGC
     3181 ACCAGGACGG CCAGGACGAA CCGTTTTTCA TTACCGAAGA GATCGAGGCG GAGATGATCG
     3241 CGGCCGGGTA CGTGTTCGAG CCGCCCGCGC ACGTCTCAAC CGTGCGGCTG CATGAAATCC
     3301 TGGCCGGTTT GTCTGATGCC AAGCTGGCGG CCTGGCCGGC CAGCTTGGCC GCTGAAGAAA
     3361 CCGAGCGCCG CCGTCTAAAA AGGTGATGTG TATTTGAGTA AAACAGCTTG CGTCATGCGG
     3421 TCGCTGCGTA TATGATGCGA TGAGTAAATA AACAAATACG CAAGGGGAAC GCATGAAGGT
     3481 TATCGCTGTA CTTAACCAGA AAGGCGGGTC AGGCAAGACG ACCATCGCAA CCCATCTAGC
     3541 CCGCGCCCTG CAACTCGCCG GGGCCGATGT TCTGTTAGTC GATTCCGATC CCCAGGGCAG
     3601 TGCCCGCGAT TGGGCGGCCG TGCGGGAAGA TCAACCGCTA ACCGTTGTCG GCATCGACCG
     3661 CCCGACGATT GACCGCGACG TGAAGGCCAT CGGCCGGCGC GACTTCGTAG TGATCGACGG
     3721 AGCGCCCCAG GCGGCGGACT TGGCTGTGTC CGCGATCAAG GCAGCCGACT TCGTGCTGAT
     3781 TCCGGTGCAG CCAAGCCCTT ACGACATATG GGCCACCGCC GACCTGGTGG AGCTGGTTAA
     3841 GCAGCGCATT GAGGTCACGG ATGGAAGGCT ACAAGCGGCC TTTGTCGTGT CGCGGGCGAT
     3901 CAAAGGCACG CGCATCGGCG GTGAGGTTGC CGAGGCGCTG GCCGGGTACG AGCTGCCCAT
     3961 TCTTGAGTCC CGTATCACGC AGCGCGTGAG CTACCCAGGC ACTGCCGCCG CCGGCACAAC
     4021 CGTTCTTGAA TCAGAACCCG AGGGCGACGC TGCCCGCGAG GTCCAGGCGC TGGCCGCTGA
     4081 AATTAAATCA AAACTCATTT GAGTTAATGA GGTAAAGAGA AAATGAGCAA AAGCACAAAC
     4141 ACGCTAAGTG CCGGCCGTCC GAGCGCACGC AGCAGCAAGG CTGCAACGTT GGCCAGCCTG
     4201 GCAGACACGC CAGCCATGAA GCGGGTCAAC TTTCAGTTGC CGGCGGAGGA TCACACCAAG
     4261 CTGAAGATGT ACGCGGTACG CCAAGGCAAG ACCATTACCG AGCTGCTATC TGAATACATC
     4321 GCGCAGCTAC CAGAGTAAAT GAGCAAATGA ATAAATGAGT AGATGAATTT TAGCGGCTAA
     4381 AGGAGGCGGC ATGGAAAATC AAGAACAACC AGGCACCGAC GCCGTGGAAT GCCCCATGTG
     4441 TGGAGGAACG GGCGGTTGGC CAGGCGTAAG CGGCTGGGTT GTCTGCCGGC CCTGCAATGG
     4501 CACTGGAACC CCCAAGCCCG AGGAATCGGC GTGACGGTCG CAAACCATCC GGCCCGGTAC
     4561 AAATCGGCGC GGCGCTGGGT GATGACCTGG TGGAGAAGTT GAAGGCCGCG CAGGCCGCCC
     4621 AGCGGCAACG CATCGAGGCA GAAGCACGCC CCGGTGAATC GTGGCAAGCG GCCGCTGATC
     4681 GAATCCGCAA AGAATCCCGG CAACCGCCGG CAGCCGGTGC GCCGTCGATT AGGAAGCCGC
     4741 CCAAGGGCGA CGAGCAACCA GATTTTTTCG TTCCGATGCT CTATGACGTG GGCACCCGCG
     4801 ATAGTCGCAG CATCATGGAC GTGGCCGTTT TCCGTCTGTC GAAGCGTGAC CGACGAGCTG
     4861 GCGAGGTGAT CCGCTACGAG CTTCCAGACG GGCACGTAGA GGTTTCCGCA GGGCCGGCCG
     4921 GCATGGCCAG TGTGTGGGAT TACGACCTGG TACTGATGGC GGTTTCCCAT CTAACCGAAT
     4981 CCATGAACCG ATACCGGGAA GGGAAGGGAG ACAAGCCCGG CCGCGTGTTC CGTCCACACG
     5041 TTGCGGACGT ACTCAAGTTC TGCCGGCGAG CCGATGGCGG AAAGCAGAAA GACGACCTGG
     5101 TAGAAACCTG CATTCGGTTA AACACCACGC ACGTTGCCAT GCAGCGTACG AAGAAGGCCA
     5161 AGAACGGCCG CCTGGTGACG GTATCCGAGG GTGAAGCCTT GATTAGCCGC TACAAGATCG
     5221 TAAAGAGCGA AACCGGGCGG CCGGAGTACA TCGAGATCGA GCTAGCTGAT TGGATGTACC
     5281 GCGAGATCAC AGAAGGCAAG AACCCGGACG TGCTGACGGT TCACCCCGAT TACTTTTTGA
     5341 TCGATCCCGG CATCGGCCGT TTTCTCTACC GCCTGGCACG CCGCGCCGCA GGCAAGGCAG
     5401 AAGCCAGATG GTTGTTCAAG ACGATCTACG AACGCAGTGG CAGCGCCGGA GAGTTCAAGA
     5461 AGTTCTGTTT CACCGTGCGC AAGCTGATCG GGTCAAATGA CCTGCCGGAG TACGATTTGA
     5521 AGGAGGAGGC GGGGCAGGCT GGCCCGATCC TAGTCATGCG CTACCGCAAC CTGATCGAGG
     5581 GCGAAGCATC CGCCGGTTCC TAATGTACGG AGCAGATGCT AGGGCAAATT GCCCTAGCAG
     5641 GGGAAAAAGG TCGAAAAGGT CTCTTTCCTG TGGATAGCAC GTACATTGGG AACCCAAAGC
     5701 CGTACATTGG GAACCGGAAC CCGTACATTG GGAACCCAAA GCCGTACATT GGGAACCGGT
     5761 CACACATGTA AGTGACTGAT ATAAAAGAGA AAAAAGGCGA TTTTTCCGCC TAAAACTCTT
     5821 TAAAACTTAT TAAAACTCTT AAAACCCGCC TGGCCTGTGC ATAACTGTCT GGCCAGCGCA
     5881 CAGCCGAAGA GCTGCAAAAA GCGCCTACCC TTCGGTCGCT GCGCTCCCTA CGCCCCGCCG
     5941 CTTCGCGTCG GCCTATCGCG GCCGCTGGCC GCTCAAAAAT GGCTGGCCTA CGGCCAGGCA
     6001 ATCTACCAGG GCGCGGACAA GCCGCGCCGT CGCCACTCGA CCGCCGGCGC CCACATCAAG
     6061 GCACCCTGCC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG
     6121 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG
     6181 TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA
     6241 GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC
     6301 GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC TCTTCCGCTT
     6361 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
     6421 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
     6481 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
     6541 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
     6601 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
     6661 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
     6721 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
     6781 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
     6841 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
     6901 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
     6961 GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
     7021 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
     7081 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
     7141 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGCATG
     7201 ATATATCTCC CAATTTGTGT AGGGCTTATT ATGCACGCTT AAAAATAATA AAAGCAGACT
     7261 TGACCTGATA GTTTGGCTGT GAGCAATTAT GTGCTTAGTG CATCTAATCG CTTGAGTTAA
     7321 CGCCGGCGAA GCGGCGTCGG CTTGAACGAA TTTCTAGCTA GACATTATTT GCCGACTACC
     7381 TTGGTGATCT CGCCTTTCAC GTAGTGGACA AATTCTTCCA ACTGATCTGC GCGCGAGGCC
     7441 AAGCGATCTT CTTCTTGTCC AAGATAAGCC TGTCTAGCTT CAAGTATGAC GGGCTGATAC
     7501 TGGGCCGGCA GGCGCTCCAT TGCCCAGTCG GCAGCGACAT CCTTCGGCGC GATTTTGCCG
     7561 GTTACTGCGC TGTACCAAAT GCGGGACAAC GTAAGCACTA CATTTCGCTC ATCGCCAGCC
     7621 CAGTCGGGCG GCGAGTTCCA TAGCGTTAAG GTTTCATTTA GCGCCTCAAA TAGATCCTGT
     7681 TCAGGAACCG GATCAAAGAG TTCCTCCGCC GCTGGACCTA CCAAGGCAAC GCTATGTTCT
     7741 CTTGCTTTTG TCAGCAAGAT AGCCAGATCA ATGTCGATCG TGGCTGGCTC GAAGATACCT
     7801 GCAAGAATGT CATTGCGCTG CCATTCTCCA AATTGCAGTT CGCGCTTAGC TGGATAACGC
     7861 CACGGAATGA TGTCGTCGTG CACAACAATG GTGACTTCTA CAGCGCGGAG AATCTCGCTC
     7921 TCTCCAGGGG AAGCCGAAGT TTCCAAAAGG TCGTTGATCA AAGCTCGCCG CGTTGTTTCA
     7981 TCAAGCCTTA CGGTCACCGT AACCAGCAAA TCAATATCAC TGTGTGGCTT CAGGCCGCCA
     8041 TCCACTGCGG AGCCGTACAA ATGTACGGCC AGCAACGTCG GTTCGAGATG GCGCTCGATG
     8101 ACGCCAACTA CCTCTGATAG TTGAGTCGAT ACTTCGGCGA TCACCGCTTC CCCCATGATG
     8161 TTTAACTTTG TTTTAGGGCG ACTGCCCTGC TGCGTAACAT CGTTGCTGCT CCATAACATC
     8221 AAACATCGAC CCACGGCGTA ACGCGCTTGC TGCTTGGATG CCCGAGGCAT AGACTGTACC
     8281 CCAAAAAAAC ATGTCATAAC AAGAAGCCAT GAAAACCGCC ACTGCGCCGT TACCACCGCT
     8341 GCGTTCGGTC AAGGTTCTGG ACCAGTTGCG TGACGGCAGT TACGCTACTT GCATTACAGC
     8401 TTACGAACCG AACGAGGCTT ATGTCCACTG GGTTCGTGCC CGAATTGATC ACAGGCAGCA
     8461 ACGCTCTGTC ATCGTTACAA TCAACATGCT ACCCTCCGCG AGATCATCCG TGTTTCAAAC
     8521 CCGGCAGCTT AGTTGCCGTT CTTCCGAATA GCATCGGTAA CATGAGCAAA GTCTGCCGCC
     8581 TTACAACGGC TCTCCCGCTG ACGCCGTCCC GGACTGATGG GCTGCCTGTA TCGAGTGGTG
     8641 ATTTTGTGCC GAGCTGCCGG TCGGGGAGCT GTTGGCTGGC TGGTGGCAGG ATATATTGTG
     8701 GTGTAAACAA ATTGACGCTT AGACAACTTA ATAACACATT GCGGACGTTT TTAATGTACT
     8761 GAATTAACGC CGAATTGAAT TATCAGCTTG CATGCCGGTC GATCTAGTAA CATAGATGAC
     8821 ACCGCGCGCG ATAATTTATC CTAGTTTGCG CGCTATATTT TGTTTTCTAT CGCGTATTAA
     8881 ATGTATAATT GCGGGACTCT AATCATAAAA ACCCATCTCA TAAATAACGT CATGCATTAC
     8941 ATGTTAATTA TTACATGCTT AACGTAATTC AACAGAAATT ATATGATAAT CATCGCAAGA
     9001 CCGGCAACAG GATTCAATCT TAAGAAACTT TATTGCCAAA TGTTTGAACG ATCTGCTTGA
     9061 CTCTAGGGAA TTAATTCCTG AATCACTGCG ACCGGCCCTC CCGCGACCCA GCCGAGCGAG
     9121 CTTAGCGAAC TGTGGACGAG AACTGTGCCA CCAAGCGTAA GGCCGTTCTC TCGCATTTGC
     9181 CTTGCTAGGC TCGCGCGAGT TGCTGGCTGA GGCGTTCTCG AAATCAGCTC TTGTTCGGTC
     9241 GGCATCTACT CTATTCCTTT GCCCTCGGAC GAGTGCTGGG GCGTCGGTTT CCACTATCGG
     9301 CGAGTACTTC TACACAGCCA TCGGTCCAGA CGGCCGCGCT TCTGCGGGCG ATTTGTGTAC
     9361 GCCCGACAGT CCCGGCTCCG GATCGGACGA TTGCGTCGCA TCGACCCTGC GCCCAAGCTG
     9421 CATCATCGAA ATTGCCGTCA ACCAAGCTCT GATAGAGTTG GTCAAGACCA ATGCGGAGCA
     9481 TATACGCCCG GAGCCTTGGC GATCCTGCAA GCTCCGGATG CCTCCGCTCG AAGTAGCGCG
     9541 TCTGCTGCTC CATACAAGCC AACCACGGCC TCCAGAAGAA GATGTTGGCG ACCTCGTATT
     9601 GGGAATCCCC GAACATCGCC TCGCTCCAGT CAATGACCGC TGTTATGCGG CCATTGTCCG
     9661 TCAGGACATT GTTGGAGCCG AATCCGCGTG CACGAGTGCC GGACTTCGGG GCAGTCCTCG
     9721 CCCAAAGCAT CAGCTCATCG AGAGCCTGCG CGACGGACGC ACTGACGGTG TCGTCCATCA
     9781 CAGTTTGCCA GTGATACACA TGGGGATCAG CAATCGCGCA TATGAAATCA CGCCATGTAG
     9841 TGTATTGACC GATTCCTTGC GGTCCGAATG GGCCGAACCC GCTCGTCTGG CTAAGATCGG
     9901 CCGCAGCGAT AGCATCCATA GCCTCCGCGA CCGGCTGAAG AACAGCGGGC AGTTCGGTTT
     9961 CAGGCAGGTC TTGCAACGTG ACACCCTGTG CACGGCGGGA GATGCAATAG GTCAGGCTCT
    10021 CGCTGAACTC CCCAATGTCA AGCACTTCCG GAATCGGGAG CGCGGCCGAT GCAAAGTGCC
    10081 GATAAACATA ACGATCTTTG TAGAAACCAT CGGCGCAGCT ATTTACCCGC AGGACATATC
    10141 CACGCCCTCC TACATCGAAG CTGAAAGCAC GAGATTCTTC GCCCTCCGAG AGCTGCATCA
    10201 GGTCGGAGAC GCTGTCGAAC TTTTCGATCA GAAACTTCTC GACAGACGTC GCGGTGAGTT
    10261 CAGGCTTTTT CATAGGGGGG ATTCGAGTTG AGAGTGAATA TGAGACTCTA ATTGGATACC
    10321 GAGGGGAATT TATGGAACGT CAGTGGAGCA TTTTTGACAA GAAATATTTG CTAGCTGATA
    10381 GTGACCTTAG GCGACTTTTG AACGCGCAAT AATGGTTTCT GACGTATGTG CTTAGCTCAT
    10441 TAAACTCCAG AAACCCGCGG CTCAGTGGCT CCTTCAACGT TGCGGTTCTG TCAGTTCCAA
    10501 ACGTAAAACG GCTTGTCCCG CGTCATCGGC GGGGGTCATA ACGTGACTCC CTTAATTCTC
    10561 CGCTCATGAT CGATAATTCG AGCTCTCCCA TATGGTCGAC CTGCAGGTCA CTGGATTTTG
    10621 GTTTTAGGAA TTAGAAATTT TATTGATAGA AGTATTTTAC AAATACAAAT ACATACTAAG
    10681 GGTTTCTTAT ATGCTCAACA CATGAGCGAA ACCCTATAAG AACCCTAATT CCCTTATCTG
    10741 GGAACTACTC ACACATTATT CTGGAGAAAA ATAGAGAGAG ATAGATTTGT AGAGAGAGAC
    10801 TGGTGATTTT TGCGGACTCT AGCATGGCCG CGGCTAGTCA GTTAGATCGT TTTCCCAGTC
    10861 ACGACGTTGT AAAACGACGG CCAGTGAATT ATCAACTATG TATAATAAAG TTGCTTACTT
    10921 GTACAGCTCG TCCATGCCGC CGGTGGAGTG GCGGCCCTCG GCGCGTTCGT ACTGTTCCAC
    10981 GATGGTGTAG TCCTCGTTGT GGGAGGTGAT GTCCAACTTG ATGTTGACGT TGTAGGCGCC
    11041 GGGCAGCTGC ACGGGCTTCT TGGCCTTGTA GGTGGTCTTG ACCTCAGCGT CGTAGTGGCC
    11101 GCCGTCCTTC AGCTTCAGCC TCTGCTTGAT CTCGCCCTTC AGGGCGCCGT CCTCGGGGTA
    11161 CATCCGCTCG GAGGAGGCCT CCCAGCCCAT GGTCTTCTTC TGCATTACGG GGCCGTCGGA
    11221 GGGGAAGTTG GTGCCGCGCA GCTTCACCTT GTAGATGAAC TCGCCGTCCT GCAGGGAGGA
    11281 GTCCTGGGTC ACGGTCACCA CGCCGCCGTC CTCGAAGTTC ATCACGCGCT CCCACTTGAA
    11341 GCCCTCGGGG AAGGACAGCT TCAAGTAGTC GGGGATGTCG GCGGGGTGCT TCACGTAGGC
    11401 CTTGGAGCCG TACATGAACT GAGGGGACAG GATGTCCCAG GCGAAGGGCA GGGGGCCACC
    11461 CTTGGTCACC TTCAGCTTGG CGGTCTGGGT GCCCTCGTAG GGGCGGCCCT CGCCCTCGCC
    11521 CTCGATCTCG AACTCGTGGC CGTTCACGGA GCCCTCCATG TGCACCTTGA AGCGCATGAA
    11581 CTCCTTGATG ATGGCCATGT TATCCTCCTC GCCCTTGCTC ACAGCCACTT TGTACAAGAA
    11641 AGCTGGGTAG TGACGTAACT GTGGTGCACG TAATGCGTGC AATCCCGCGC GAATTGCATC
    11701 CAGGTCTGCC GGGCGCATTG CCTGCTGGGC CAGTAAGTAC CCGCGAGCGA ACTGTACGGC
    11761 TTCTGCGGCT TGAGGTTGTT GTTGGGCTAA TTCCTGTAAT AAACGTCCCG CTACTACCAA
    11821 GTCGTTATGG CGTCCAAGTT CCTCCTGGGT TGCTGCCAAA GCTTTCAGAT ATGCACGGGT
    11881 TGATTTTGAG CGATACAAAC CATGGAAGAA CTCTAAAGCA TAGCGACCAC GCTTGGTGGC
    11941 GATGCGGGTA CGGTGAGCGG ATGCTGCGTC ATCGTCCTGC ATGCGAGCTG CACGCTTCAA
    12001 TAAGCTTTTG TGAAGATGTT GCATGATTTG ACGAGAGAAA TGAGCGGCTG ACCCGTCTAA
    12061 TAATGGGGCT GTTTCAAGCA TCCAAGCTCC CAGCGTTAAC ATCAAACGTG TGTAACGTGG
    12121 CGATAACAGA GCCTGTGCAG CTGCATGACG CTTTGCCTGG GCAATTTTTT GTACTAATGC
    12181 ATTAAGCTCC AACAAGCCGT TGGCCCCCGC AGGCGCTCCA TCGATACGTG GCAGTGTAGA
    12241 TGCCAGCAGA ACATCCCAAT CACGCGCTGC CCCCAGCTCC GTTCCCAGCC AAGAAATATC
    12301 ATCCTGAAGG GCAGGCGGGC ATGGCGCGAC TGCGTCAAAT AATTTTAAAG CAGAACGAAG
    12361 GCGACGGACT CCAACACGCA TTTGGTGCAG CGTTTCGGGG TCATGCCCTT CAATAACCGC
    12421 GACCTCATTA CGCTGAATGT GTTCTAAGCA GTTTCCCAGG ATCGCAGGAA ACGCTTCACT
    12481 CAGAGCGGCG TCGGCGTCAA GTGCTAATGC TTGAGCGCGG AATGGGGCAT GCCCTGTCTC
    12541 GCGAACAAGC ATTAAGCCTG CTTT
//
