LOCUS       pNapin-dsRed            8953 bp    DNA     circular     25-JAN-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|709694667|
COMMENT     VNTDBDATE|-12131462|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pNapin-dsRed|
COMMENT     VNTAUTHORNAME|JZ|
FEATURES             Location/Qualifiers
     CDS             7659..8336
                     /vntifkey="4"
                     /label=dsRed
     promoter        6507..7658
                     /vntifkey="29"
                     /label=Napin\Promoter
     misc_feature    6173..6198
                     /vntifkey="21"
                     /label=T-DNA\Left\Border
     misc_feature    8867..8892
                     /vntifkey="21"
                     /label=T-DNA\Right\Border
     misc_feature    complement(980..1980)
                     /vntifkey="21"
                     /label=pVS1\STA
     CDS             complement(4957..5748)
                     /vntifkey="4"
                     /label=Kanamycin\R
     rep_origin      complement(4383..4663)
                     /vntifkey="33"
                     /label=Replication\Origin\pBR322
     rep_origin      complement(2573..3573)
                     /vntifkey="33"
                     /label=pVS1\Replication\Origin
     terminator      8337..8589
                     /vntifkey="43"
                     /label=NOS?
     misc_feature    7090..7110
                     /vntifkey="21"
                     /label=Napinseq_A
     misc_feature    8476..8498
                     /vntifkey="21"
                     /label=Napinseq_C
     misc_feature    complement(5209..5229)
                     /vntifkey="21"
                     /label=NapinSeq_E
     misc_feature    complement(4493..4513)
                     /vntifkey="21"
                     /label=NapinSeq_F
     misc_feature    complement(3836..3856)
                     /vntifkey="21"
                     /label=NapinSeq_G
     misc_feature    complement(3146..3166)
                     /vntifkey="21"
                     /label=NapinSeq_H
     misc_feature    complement(2423..2442)
                     /vntifkey="21"
                     /label=NapinSeq_I
     misc_feature    complement(1760..1780)
                     /vntifkey="21"
                     /label=NapinSeq_J
     misc_feature    complement(1052..1071)
                     /vntifkey="21"
                     /label=NapinSeq_K
     misc_feature    complement(337..356)
                     /vntifkey="21"
                     /label=NapinSeq_L
     misc_feature    complement(8638..8660)
                     /vntifkey="21"
                     /label=M13/pUC\Forward
     misc_feature    complement(5901..5921)
                     /vntifkey="21"
                     /label=NapinSeq_D
BASE COUNT     2246 a      2341 c      2272 g      2094 t 
ORIGIN
        1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct 
       61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca 
      121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt 
      181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca 
      241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga 
      301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca 
      361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg 
      421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca 
      481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg 
      541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg 
      601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg 
      661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga 
      721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga 
      781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg 
      841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac 
      901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac 
      961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt 
     1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg 
     1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt 
     1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca 
     1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc 
     1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg 
     1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa 
     1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc 
     1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg 
     1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc 
     1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa 
     1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag 
     1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac 
     1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc 
     1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta 
     1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca 
     1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc 
     1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca 
     2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa 
     2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc 
     2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc 
     2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga 
     2281 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga 
     2341 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg 
     2401 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc 
     2461 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc 
     2521 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg 
     2581 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca 
     2641 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact 
     2701 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa 
     2761 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga 
     2821 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt 
     2881 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga 
     2941 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga 
     3001 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct 
     3061 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct 
     3121 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg 
     3181 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc 
     3241 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt 
     3301 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca 
     3361 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga 
     3421 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa 
     3481 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa 
     3541 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc 
     3601 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg 
     3661 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc 
     3721 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc 
     3781 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg 
     3841 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 
     3901 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca 
     3961 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca 
     4021 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 
     4081 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 
     4141 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 
     4201 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 
     4261 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 
     4321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 
     4381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 
     4441 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 
     4501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 
     4561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 
     4621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 
     4681 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 
     4741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 
     4801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 
     4861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 
     4921 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata 
     4981 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata 
     5041 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt 
     5101 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa 
     5161 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt 
     5221 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc 
     5281 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc 
     5341 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc 
     5401 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag 
     5461 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag 
     5521 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac 
     5581 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc 
     5641 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta 
     5701 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct 
     5761 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc 
     5821 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg 
     5881 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca 
     5941 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt 
     6001 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag 
     6061 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat 
     6121 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga 
     6181 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt 
     6241 taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg 
     6301 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag 
     6361 ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg 
     6421 ggaactactc acacattatt atggagaaac tcgagaggaa gcttgcatgc tgcaggtcga 
     6481 ctctagagga tccccgggac cgagctcgaa ttcgattcgg agctctcttc atcggtgatt 
     6541 gattccttta aagacttatg tttcttatct tgcttctgag gcaagtattc agttaccagt 
     6601 taccacttat attctgactt tctgactgca tcctcatttt tccaacattt taaatttcat 
     6661 attggctgaa tgcttcttct ttgaggaaga aacaattcag atggcagaaa tgtatcaacc 
     6721 aatgcatata tacaaatgta cctcttgttc tcaaaacatc tatcggatgg ttccatttgc 
     6781 tttgtcatcc aattagtgac tactttatat tattcactcc tttttattac tatttcatgg 
     6841 aggttgccat gtacattata tttgtaagga tgacgctatt gagcgttttt cttcaatttt 
     6901 ctttatttta gacatgggta tgaaatgtgt gttagagttg ggttgaatga gatatacgtt 
     6961 caagtgaagt ggcataccgt tgtcgagtaa ggatgaccta cccattcttg agacaaatgt 
     7021 tacattttag tatcagagta aaatgtgtac ctataactca aattcgattg acatgtatcc 
     7081 attcaacata aaattaaacc agcctgcacc tgcatccaca tttcaagtat tttcaaaccg 
     7141 ttcggctcct atccaccggg tgtaacaaga cggattccga atttggaaga ttttgactca 
     7201 aattcccaat ttatattgac cgtgactaaa tcaactttaa cttctataat tctgattaag 
     7261 ctcccaattt atattcccaa cggcactacc tccaaaattt atagactctc atcccctttt 
     7321 aaaccaactt agtaaacgct ctttttttta attttatgaa gttaagtttt taccttgttt 
     7381 ttaaaaagaa tcgttcataa gatgccatgc cagaacatta gctacacgtt acacatagca 
     7441 tgcagccgcg gagaattgtt tttcttcgcc acttgtcact cccttcaaac acctaagagc 
     7501 ttctctctca cagcacacac atacaatcac atgcgtgcat gcattattac acgtgatcgc 
     7561 catgcaaatc tcctttatag tctataaatt aactcatccg cttcactctt tactcaaacc 
     7621 aaaactcatc aatacaaaca agattaaaaa cataccccat gcggtcttcc aagaatgtta 
     7681 tcaaggagtt catgaggttt aaggttcgca tggaaggaac ggtcaatggg cacgagtttg 
     7741 aaatagaagg cgaaggagag gggaggccat acgaaggcca caataccgta aagcttaagg 
     7801 taaccaaggg gggacctttg ccatttgctt gggatatttt gtcaccacaa tttcagtatg 
     7861 gaagcaaggt atatgtcaag caccctgccg acataccaga ctataaaaag ctgtcatttc 
     7921 ctgaaggatt taaatgggaa agggtcatga actttgaaga cggtggcgtc gttactgtaa 
     7981 cccaggattc cagtttgcag gatggctgtt tcatctacaa ggtcaagttc attggcgtga 
     8041 actttccttc cgatggacct gttatgcaaa agaagacaat gggctgggaa gccagcactg 
     8101 agcgtttgta tcctcgtgat ggcgtgttga aaggagagat tcataaggct ctgaagctga 
     8161 aagacggtgg tcattaccta gttgaattca aaagtattta catggcaaag aagcctgtgc 
     8221 agctaccagg gtactactat gttgactcca aactggatat aacaagccac aacgaagact 
     8281 atacaatcgt tgagcagtat gaaagaaccg agggacgcca ccatctgttc ctttaagatc 
     8341 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga 
     8401 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga 
     8461 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga 
     8521 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt 
     8581 tactagatcg gatcctctag agtcgacctg caggcatgca agcttggcac tggccgtcgt 
     8641 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca 
     8701 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca 
     8761 gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc 
     8821 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga 
     8881 aaagagcgtt tattagaata acggatattt aaaagggcgt gaaaaggttt atccgttcgt 
     8941 ccatttgtat gtg 
//
