LOCUS       Exported                5993 bp ds-DNA     circular SYN 21-FEB-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Groudine Lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 22, 2019 from SnapGene 4.3.8.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      complement(43..631)
                     /direction=LEFT
                     /label=Rep_Origin_1
                     /note="Col E1 origin of replication"
                     /note="color: #ff9966"
     primer_bind     complement(83..102)
                     /label=LJT183
                     /note="color: black; sequence: cacctctgacttgagcgtcg"
     gene            complement(806..1593)
                     /label=beta lactamase
                     /note="beta lactamase (amp resistance)"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     893..912
                     /label=LJT245
                     /note="color: black; sequence: acgatacgggagggcttacc"
     primer_bind     894..915
                     /label=LJT068
                     /note="color: black; sequence: cgatacgggagggcttaccatc"
     primer_bind     complement(897..918)
                     /label=LJT067
                     /note="color: black; sequence: ccagatggtaagccctcccgta"
     primer_bind     complement(924..943)
                     /label=LJT129
                     /note="color: black; sequence: tcgcggtatcattgcagcac"
     primer_bind     1697..1719
                     /label=LJT151
                     /note="color: black; sequence: CAGGGTTATTGTCTCATGAGCGG"
     primer_bind     complement(1792..1816)
                     /label=pKO Vector.REV
                     /note="color: black; sequence: ccatggtcatagctgtttgacgtca"
     primer_bind     complement(1794..1826)
                     /label=LJT943
                     /note="color: black; sequence: 
                     ggtaccaatgccatggtcatagctgtttgacgt"
     misc_feature    1800..1815
                     /label=M13 reverse primer
                     /label=M13 reverse primer (-24)
                     /note="Reverse Primer"
                     /note="color: #3366cc; direction: RIGHT"
     primer_bind     1807..1845
                     /label=LJT936
                     /note="color: black; sequence: 
                     atgaccatggcattggtaccattatctacattcctcccc"
     gene            1814..1816
                     /gene="CBX5"
                     /gene_synonym="HEL25; HP1; HP1A"
                     /label=CBX5
                     /note="chromobox 5; Derived by automated computational 
                     analysis using gene prediction method: BestRefSeq."
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:23468"
                     /db_xref="HGNC:HGNC:1555"
                     /db_xref="HPRD:05131"
                     /db_xref="MIM:604478"
     misc_feature    1817..2815
                     /label=Mouse Npm1 Left H.A.
                     /note="color: #00ffff"
     gene            2586..2816
                     /gene="Npm1"
                     /gene_synonym="B23; NO38; Npm"
                     /label=Npm1
                     /note="nucleophosmin 1; Derived by automated computational 
                     analysis using gene prediction method: BestRefSeq."
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:18148"
                     /db_xref="MGI:MGI:106184"
     mRNA            2586..2816
                     /gene="Npm1"
                     /gene_synonym="B23; NO38; Npm"
                     /product="nucleophosmin 1, transcript variant 2"
                     /label=Npm1 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /note="/transcript_id=NM_001252260.1"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:18148"
                     /db_xref="MGI:MGI:106184"
     mRNA            2586..2816
                     /gene="Npm1"
                     /gene_synonym="B23; NO38; Npm"
                     /product="nucleophosmin 1, transcript variant 1"
                     /label=Npm1 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /note="/transcript_id=NM_008722.3"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:18148"
                     /db_xref="MGI:MGI:106184"
     mRNA            2586..2816
                     /gene="Npm1"
                     /gene_synonym="B23; NO38; Npm"
                     /product="nucleophosmin 1, transcript variant 3"
                     /label=Npm1 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /note="/transcript_id=NM_001252261.1"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:18148"
                     /db_xref="MGI:MGI:106184"
     primer_bind     complement(2801..2826)
                     /label=LJT937
                     /note="color: black; sequence: tactcggtggccatgaggtggaggcg"
     primer_bind     2806..2831
                     /label=LJT938
                     /note="color: black; sequence: ccacctcatggccaccgagtacaagc"
     gene            2812..2816
                     /gene="CBX5"
                     /gene_synonym="HEL25; HP1; HP1A"
                     /label=CBX5
                     /note="chromobox 5; Derived by automated computational 
                     analysis using gene prediction method: BestRefSeq."
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:23468"
                     /db_xref="HGNC:HGNC:1555"
                     /db_xref="HPRD:05131"
                     /db_xref="MIM:604478"
     mRNA            2812..2816
                     /gene="CBX5"
                     /gene_synonym="HEL25; HP1; HP1A"
                     /product="chromobox 5, transcript variant 3"
                     /label=CBX5 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq.
                     transcript_id=NM_012117.2"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:23468"
                     /db_xref="HGNC:HGNC:1555"
                     /db_xref="HPRD:05131"
                     /db_xref="MIM:604478"
     mRNA            2812..2816
                     /gene="CBX5"
                     /gene_synonym="HEL25; HP1; HP1A"
                     /product="chromobox 5, transcript variant 2"
                     /label=CBX5 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq.
                     transcript_id=NM_001127321.1"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:23468"
                     /db_xref="HGNC:HGNC:1555"
                     /db_xref="HPRD:05131"
                     /db_xref="MIM:604478"
     mRNA            2812..2816
                     /gene="CBX5"
                     /gene_synonym="HEL25; HP1; HP1A"
                     /product="chromobox 5, transcript variant 1"
                     /label=CBX5 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq.
                     transcript_id=NM_001127322.1"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:23468"
                     /db_xref="HGNC:HGNC:1555"
                     /db_xref="HPRD:05131"
                     /db_xref="MIM:604478"
     misc_feature    2812..2815
                     /label=Human Hp1a Left H.A.
                     /note="color: #ffff99"
     misc_feature    2812
                     /label=Kozak
                     /note="color: #a6acb3"
     CDS             2813..2816
                     /codon_start=1
                     /gene="CBX5"
                     /gene_synonym="HEL25; HP1; HP1A"
                     /product="chromobox protein homolog 5"
                     /label=CBX5
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS8875.1"
                     /db_xref="GeneID:23468"
                     /db_xref="HGNC:HGNC:1555"
                     /db_xref="HPRD:05131"
                     /db_xref="MIM:604478"
                     /protein_id="NP_001120793.1"
                     /translation="M"
     CDS             2813..2816
                     /codon_start=1
                     /gene="CBX5"
                     /gene_synonym="HEL25; HP1; HP1A"
                     /product="chromobox protein homolog 5"
                     /label=CBX5
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS8875.1"
                     /db_xref="GeneID:23468"
                     /db_xref="HGNC:HGNC:1555"
                     /db_xref="HPRD:05131"
                     /db_xref="MIM:604478"
                     /protein_id="NP_001120794.1"
                     /translation="M"
     CDS             2813..2816
                     /codon_start=1
                     /gene="CBX5"
                     /gene_synonym="HEL25; HP1; HP1A"
                     /product="chromobox protein homolog 5"
                     /label=CBX5
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS8875.1"
                     /db_xref="GeneID:23468"
                     /db_xref="HGNC:HGNC:1555"
                     /db_xref="HPRD:05131"
                     /db_xref="MIM:604478"
                     /protein_id="NP_036249.1"
                     /translation="M"
     CDS             2813..2816
                     /codon_start=1
                     /gene="Npm1"
                     /gene_synonym="B23; NO38; Npm"
                     /product="nucleophosmin isoform 2"
                     /label=Npm1
                     /note="isoform 2 is encoded by transcript variant 2; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /note="color: #993366"
                     /db_xref="GeneID:18148"
                     /db_xref="MGI:MGI:106184"
                     /protein_id="NP_001239189.1"
                     /translation="M"
     CDS             2813..2816
                     /codon_start=1
                     /gene="Npm1"
                     /gene_synonym="B23; NO38; Npm"
                     /product="nucleophosmin isoform 1"
                     /label=Npm1
                     /note="isoform 1 is encoded by transcript variant 1; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS24532.1"
                     /db_xref="GeneID:18148"
                     /db_xref="MGI:MGI:106184"
                     /protein_id="NP_032748.1"
                     /translation="M"
     CDS             2813..2816
                     /codon_start=1
                     /gene="Npm1"
                     /gene_synonym="B23; NO38; Npm"
                     /product="nucleophosmin isoform 3"
                     /label=Npm1
                     /note="isoform 3 is encoded by transcript variant 3; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS56765.1"
                     /db_xref="GeneID:18148"
                     /db_xref="MGI:MGI:106184"
                     /protein_id="NP_001239190.1"
                     /translation="M"
     CDS             2816..3412
                     /codon_start=3
                     /label=puroR
                     /note="color: #008000"
                     /translation="ATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    2816
                     /label=Human Hp1a Right H.A.
                     /note="color: #ffff99"
     misc_feature    2816
                     /label=Mouse Npm1 Right H.A.
                     /note="color: #00ffff"
     primer_bind     complement(2818..2842)
                     /label=LJT009
                     /note="color: black; sequence: Gcgcaccgtgggcttgtactcggtg"
     primer_bind     2823..2840
                     /label=LJT344
                     /note="color: black; sequence: agtacaagcccacggtgc"
     primer_bind     complement(2924..2941)
                     /label=LJT139
                     /note="color: black; sequence: gtggcggtccggatcgac"
     primer_bind     complement(2959..2980)
                     /label=LJT232
                     /note="color: black; sequence: cgtgaggaagagttcttgcagc"
     primer_bind     3321..3339
                     /label=LJT096
                     /note="color: black; sequence: ccttctacgagcggctcgg"
     primer_bind     3381..3398
                     /label=LJT243
                     /note="color: black; sequence: gcacctggtgcatgaccc"
     CDS             3413..3476
                     /codon_start=1
                     /label=T2A
                     /note="color: #008000"
                     /translation="SGEGRGSLLTCGDVEENPGPM"
     CDS             3479..4195
                     /codon_start=1
                     /label=sfGFP
                     /label=sfGFP-TetRx2
                     /note="color: #008000"
                     /translation="SVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTITFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3542..3564)
                     /label=LJT346
                     /note="color: black; sequence: ctgaacttgtggccgtttacgtc"
     primer_bind     3542..3564
                     /label=LJT345
                     /note="color: black; sequence: gacgtaaacggccacaagttcag"
     primer_bind     3817..3835
                     /label=LJT097
                     /note="color: black; sequence: gaagttcgagggcgacacc"
     primer_bind     3942..3961
                     /label=LJT235
                     /note="color: black; sequence: CCGACAAGCAGAAGAACGGC"
     CDS             4205..4885
                     /codon_start=1
                     /product="Arabidopsis IAA17 AID domain (codon optimized)"
                     /label=IAA17 AID domain
                     /note="color: #ff0000"
                     /translation="GSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNNE
                     PANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGG
                     PEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMNE
                     RKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAMEK
                     CKSRA"
     primer_bind     complement(4211..4233)
                     /label=LJT011
                     /note="color: black; sequence: GTCTCGCGCAGGTTCAGTTCCAC"
     primer_bind     4388..4407
                     /label=LJT098
                     /note="color: black; sequence: AGCAAAGAGAAGTCCGCCTG"
     primer_bind     4480..4499
                     /label=LJT144
                     /note="color: black; sequence: GAACGTGATGGTGTCCTGCC"
     primer_bind     4859..4881
                     /label=LJT010
                     /note="color: black; sequence: GCTATGGAAAAGTGCAAGAGCAG"
     primer_bind     complement(4891..4919)
                     /label=LJT939
                     /note="color: black; sequence: 
                     tcgagtcttcgGATCCAGAGCCTCCAGAC"
     primer_bind     4900..4936
                     /label=LJT940
                     /note="color: black; sequence: 
                     CTCTGGATCcgaagactcgatggatatggacatgagt"
     gene            4910..5909
                     /gene="Npm1"
                     /gene_synonym="B23; NO38; Npm"
                     /label=Npm1
                     /note="nucleophosmin 1; Derived by automated computational 
                     analysis using gene prediction method: BestRefSeq."
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:18148"
                     /db_xref="MGI:MGI:106184"
     misc_feature    4910..5909
                     /label=Mouse Npm1 Right H.A.
                     /note="color: #00ffff"
     mRNA            4910..4964
                     /gene="Npm1"
                     /gene_synonym="B23; NO38; Npm"
                     /product="nucleophosmin 1, transcript variant 2"
                     /label=Npm1 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /note="/transcript_id=NM_001252260.1"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:18148"
                     /db_xref="MGI:MGI:106184"
     mRNA            4910..4964
                     /gene="Npm1"
                     /gene_synonym="B23; NO38; Npm"
                     /product="nucleophosmin 1, transcript variant 1"
                     /label=Npm1 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /note="/transcript_id=NM_008722.3"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:18148"
                     /db_xref="MGI:MGI:106184"
     mRNA            4910..4964
                     /gene="Npm1"
                     /gene_synonym="B23; NO38; Npm"
                     /product="nucleophosmin 1, transcript variant 3"
                     /label=Npm1 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /note="/transcript_id=NM_001252261.1"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:18148"
                     /db_xref="MGI:MGI:106184"
     CDS             4910..4964
                     /codon_start=1
                     /gene="Npm1"
                     /gene_synonym="B23; NO38; Npm"
                     /product="nucleophosmin isoform 2"
                     /label=Npm1
                     /note="isoform 2 is encoded by transcript variant 2; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /note="color: #993366"
                     /db_xref="GeneID:18148"
                     /db_xref="MGI:MGI:106184"
                     /protein_id="NP_001239189.1"
                     /translation="EDSMDMDMSPLRPQNYLF"
     CDS             4910..4964
                     /codon_start=1
                     /gene="Npm1"
                     /gene_synonym="B23; NO38; Npm"
                     /product="nucleophosmin isoform 1"
                     /label=Npm1
                     /note="isoform 1 is encoded by transcript variant 1; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS24532.1"
                     /db_xref="GeneID:18148"
                     /db_xref="MGI:MGI:106184"
                     /protein_id="NP_032748.1"
                     /translation="EDSMDMDMSPLRPQNYLF"
     CDS             4910..4964
                     /codon_start=1
                     /gene="Npm1"
                     /gene_synonym="B23; NO38; Npm"
                     /product="nucleophosmin isoform 3"
                     /label=Npm1
                     /note="isoform 3 is encoded by transcript variant 3; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS56765.1"
                     /db_xref="GeneID:18148"
                     /db_xref="MGI:MGI:106184"
                     /protein_id="NP_001239190.1"
                     /translation="EDSMDMDMSPLRPQNYLF"
     gene            4910
                     /gene="CBX5"
                     /gene_synonym="HEL25; HP1; HP1A"
                     /label=CBX5
                     /note="chromobox 5; Derived by automated computational 
                     analysis using gene prediction method: BestRefSeq."
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:23468"
                     /db_xref="HGNC:HGNC:1555"
                     /db_xref="HPRD:05131"
                     /db_xref="MIM:604478"
     mRNA            4910
                     /gene="CBX5"
                     /gene_synonym="HEL25; HP1; HP1A"
                     /product="chromobox 5, transcript variant 3"
                     /label=CBX5 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq.
                     transcript_id=NM_012117.2"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:23468"
                     /db_xref="HGNC:HGNC:1555"
                     /db_xref="HPRD:05131"
                     /db_xref="MIM:604478"
     mRNA            4910
                     /gene="CBX5"
                     /gene_synonym="HEL25; HP1; HP1A"
                     /product="chromobox 5, transcript variant 2"
                     /label=CBX5 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq.
                     transcript_id=NM_001127321.1"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:23468"
                     /db_xref="HGNC:HGNC:1555"
                     /db_xref="HPRD:05131"
                     /db_xref="MIM:604478"
     mRNA            4910
                     /gene="CBX5"
                     /gene_synonym="HEL25; HP1; HP1A"
                     /product="chromobox 5, transcript variant 1"
                     /label=CBX5 mRNA
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq.
                     transcript_id=NM_001127322.1"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:23468"
                     /db_xref="HGNC:HGNC:1555"
                     /db_xref="HPRD:05131"
                     /db_xref="MIM:604478"
     CDS             4910
                     /codon_start=1
                     /gene="CBX5"
                     /gene_synonym="HEL25; HP1; HP1A"
                     /product="chromobox protein homolog 5"
                     /label=CBX5
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS8875.1"
                     /db_xref="GeneID:23468"
                     /db_xref="HGNC:HGNC:1555"
                     /db_xref="HPRD:05131"
                     /db_xref="MIM:604478"
                     /protein_id="NP_001120793.1"
                     /translation=""
     CDS             4910
                     /codon_start=1
                     /gene="CBX5"
                     /gene_synonym="HEL25; HP1; HP1A"
                     /product="chromobox protein homolog 5"
                     /label=CBX5
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS8875.1"
                     /db_xref="GeneID:23468"
                     /db_xref="HGNC:HGNC:1555"
                     /db_xref="HPRD:05131"
                     /db_xref="MIM:604478"
                     /protein_id="NP_001120794.1"
                     /translation=""
     CDS             4910
                     /codon_start=1
                     /gene="CBX5"
                     /gene_synonym="HEL25; HP1; HP1A"
                     /product="chromobox protein homolog 5"
                     /label=CBX5
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS8875.1"
                     /db_xref="GeneID:23468"
                     /db_xref="HGNC:HGNC:1555"
                     /db_xref="HPRD:05131"
                     /db_xref="MIM:604478"
                     /protein_id="NP_036249.1"
                     /translation=""
     misc_feature    4910
                     /label=Human Hp1a Right H.A.
                     /note="color: #ffff99"
     primer_bind     complement(5881..5919)
                     /label=LJT941
                     /note="color: black; sequence: 
                     ggggcgcgccctatagatgtgatccccaaaagccttgat"
     primer_bind     5900..5921
                     /label=LJT942
                     /note="color: black; sequence: acatctatagggcgcgcccctc"
     misc_feature    complement(5910..5993)
                     /label=Misc_Feature_1
                     /note="scrambler polylinker"
                     /note="color: #66cc99; direction: LEFT"
     primer_bind     5910..5922
                     /label=pKO Vector.FOR
                     /note="color: black; sequence: ggcgcgcccctcg"
     gene            5910..5911
                     /gene="CBX5"
                     /gene_synonym="HEL25; HP1; HP1A"
                     /label=CBX5
                     /note="chromobox 5; Derived by automated computational 
                     analysis using gene prediction method: BestRefSeq."
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:23468"
                     /db_xref="HGNC:HGNC:1555"
                     /db_xref="HPRD:05131"
                     /db_xref="MIM:604478"
     primer_bind     complement(5970..5986)
                     /label=M13 Universal
                     /note="color: #009966; direction: LEFT"
ORIGIN
        1 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
       61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
      241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      481 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
      841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
      901 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
      961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcaa
     1801 acagctatga ccatggcatt ggtaccatta tctacattcc tcccccaaat tcttaaatat
     1861 gctagctcca tgttgagata aaaagattag ggccgctgtc agaaagcacc caacgctgag
     1921 ggatgttcgt caggattcat tttaaagcgc taattcccag ccgaagattc gtattaaggc
     1981 tcatgaattt ctaaaaaagc gataggcctt catttggaat gatgaagacg gcctgcgttt
     2041 tctcagttct gcacgctcta aagttttaga aagatgctct ttggaggcga ctgactgggt
     2101 ttctctttgg tccccaaatg acataaagct gataattttc gttttgaaaa tgctgcctca
     2161 gaaaagaggt gcaaaagcat ggctgcgggg aaagtagcct agatccagcc actccgccct
     2221 gggctcctga agctacgtga aaacgaagag caagccttcg agcaagccaa atcctgaggt
     2281 gattatgctt gttccaggcc tacggggaac ggagggtgga gaaaagagac gctctgtggt
     2341 tccctggctg cagccgccat ctttcagggt gggctgcgca cgctctcggc cctgggcccg
     2401 cgagcacgag cggagaagac gtggaagccc tgaaaaccca gagcgaggcc acaaagagaa
     2461 aacgcgcctg cgcttgggcg acagtagtgg agggacgggg ccagagacga acgcgcgtgc
     2521 gcagtgagag cccgccaagc acctggagga ggaggggggc gaggtaccta ggcgtgagga
     2581 tgaaaggcgc acgcgcaaaa gcgcgactgt gcggggagtg ggagggatcc agaggaggct
     2641 cgcggaggca ggactagcgc agatctcgcg agatctcctg cgaccatata tacgtgcgga
     2701 gagcccgcgt cctttccttg gcgtgattcc gtcctgcgcg tctgttctgt ggaacaggag
     2761 gcagttgttt tccgtccggc ttctcccaca ccgaagtgcg cgcctccacc tcatggccac
     2821 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     2881 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     2941 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     3001 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     3061 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     3121 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     3181 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     3241 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     3301 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     3361 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagcggtga
     3421 aggcagaggc tctctcctca catgtgggga tgttgaagaa aatccaggtc ccatgtctag
     3481 cgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     3541 cgacgtaaac ggccacaagt tcagcgtgcg cggcgagggc gagggcgatg ccaccaacgg
     3601 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     3661 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     3721 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcacctt
     3781 caaggacgac ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     3841 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     3901 gctggagtac aacttcaaca gccacaacgt ctatatcacc gccgacaagc agaagaacgg
     3961 catcaaggcc aacttcaaga tccgccacaa cgtggaggac ggcagcgtgc agctcgccga
     4021 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     4081 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     4141 gctggagttc gtgaccgccg ccgggatcac tcacggcatg gacgagctgt acaagggagg
     4201 taccggcagc gtggaactga acctgcgcga gacagagctg tgcctgggac tgcctggcgg
     4261 cgatacagtg gctcctgtga ccggcaacaa gagaggcttc agcgagacag tggacctgaa
     4321 gctgaacctg aacaacgagc ccgccaacaa agagggcagc accacccacg acgtcgtgac
     4381 cttcgacagc aaagagaagt ccgcctgccc caaggacccc gccaagcctc ctgctaaagc
     4441 tcaggtcgtg ggatggcctc ccgtgcggag ctacagaaag aacgtgatgg tgtcctgcca
     4501 gaagtcctct ggcggccctg aggctgccgc cttcgtgaaa gtgtctatgg atggcgcccc
     4561 ttacctgaga aagatcgacc tgagaatgta caagagctac gacgagctga gcaacgccct
     4621 gagcaacatg ttcagcagct tcacaatggg caagcacggc ggcgaggaag gcatgatcga
     4681 cttcatgaac gagcggaagc tgatggacct cgtgaacagc tgggactacg tgcccagcta
     4741 cgaggacaag gacggcgact ggatgctcgt gggagatgtg ccctggccca tgttcgtgga
     4801 cacatgcaag agactgagac tgatgaaggg cagcgacgcc atcggcctgg cccccagagc
     4861 tatggaaaag tgcaagagca gagccggagg gtctggaggc tctggatccg aagactcgat
     4921 ggatatggac atgagtcctc ttaggcctca gaactacctt ttcggtaact gactggaggg
     4981 ggaagcgtgt gcagggcctg gtgatggggg ggggggcggg agcccagccg aggcctggcg
     5041 tgtggggaac gcggcgtagg tggaggccgg agagcaagga ggaatctccc aggccttaga
     5101 agcagcggcc tgaacactag gaaagccgcc ggaaaatggg gcgggagcgg cccagcctgt
     5161 agcctcgggt gttaggagcg gccggccggc cggcgagggt gcgaccttgc ctgtggcgcg
     5221 aacttgagag gcgtttctcc tgtgcacgaa agcccgcacg gtcgcttccg agctcctttt
     5281 tttcctcttg tgttgagcca cggcaggcgg cacgggagaa aggggcctgt ggagcgaggc
     5341 gaggcctacc tccgccctgt tggggaggat acatctgcag gcgctgggct ttccaggggc
     5401 cgcatcttgt attctgtggc gaccgagggc ttcgcccagt aacggggaat ggtccgagca
     5461 ggcgccctga ggcagaaggg gcagcaaccc ctaggagcac gtggttgcca cgtggttggg
     5521 ggttagggag ggggtgtggg ctctacatcc gggctctccg actctagcca accttcagcc
     5581 gtgggatctt gtggcttagt taaaagttct gggagggaaa tgtttatttc atagcccgtg
     5641 gcgtatgggt ttttgagatc tttgttcata tctttcagta tccacagtga tggagccccc
     5701 cctttttaat ttgcctctgg taggcggagt cagcatcacg ttgcagatag ctgtactgat
     5761 ggcccacgtg gcttaatgtt aggagtaccc ttaaatcatc tacctggctc tatctagaac
     5821 tactttttct tggggtgggg agagggaagg atctgttgaa cagacaagat tcgctttagc
     5881 atcaaggctt ttggggatca catctatagg gcgcgcccct cgagctagaa gatctcctag
     5941 aggtacccta gagaattcct agagttaaca ctggccgtcg ttttaccggt ccg
//
