LOCUS       Exported                7016 bp ds-DNA     circular SYN 20-OCT-2013
DEFINITION  -.
ACCESSION   -
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 22, 2019 from SnapGene 4.3.8.1
            https://www.snapgene.com
COMMENT     This file is created by Vector NTI http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          674..1628
                     /organism="Mus musculus"
                     /chromosome="7"
                     /strain="C57BL/6J"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:10090"
     source          3723..4794
                     /organism="Mus musculus"
                     /chromosome="7"
                     /strain="C57BL/6J"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:10090"
     rep_origin      138..444
                     /label=F1\origin
                     /note="f1 (+) origin"
                     /note="color: #a6acb3"
     primer_bind     603..619
                     /label=M13 For (-20)
                     /label=M13\Universal\primer\(-20)
                     /note="color: #a6acb3; direction: RIGHT"
     gene            674..1628
                     /gene="Srcap"
                     /label=Srcap
                     /note="Derived by automated computational analysis using 
                     gene prediction method: GNOMON. Supporting evidence 
                     includes similarity to: 4 mRNAs, 76 ESTs, 3 Proteins"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:100043597"
                     /db_xref="MGI:2444036"
     CDS             1626..1628
                     /codon_start=1
                     /gene="Srcap"
                     /product="helicase SRCAP"
                     /label=Srcap
                     /note="Derived by automated computational analysis using 
                     gene prediction method: GNOMON."
                     /note="color: #993366"
                     /db_xref="GI:309268993"
                     /db_xref="GeneID:100043597"
                     /db_xref="MGI:2444036"
                     /protein_id="XP_003084784.1"
                     /translation="M"
     CDS             1630..2225
                     /codon_start=1
                     /label=puroR
                     /note="color: #008000"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             2226..2289
                     /codon_start=1
                     /label=T2A
                     /note="color: #008000"
                     /translation="SGEGRGSLLTCGDVEENPGPM"
     CDS             2292..3008
                     /codon_start=1
                     /label=sfGFP
                     /label=sfGFP-TetRx2
                     /note="color: #008000"
                     /translation="SVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTITFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             3018..3698
                     /codon_start=1
                     /label=Arabidopsis IAA17 AID domain (codon optimized)
                     /note="color: #a6acb3"
                     /translation="GSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNNE
                     PANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGG
                     PEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMNE
                     RKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAMEK
                     CKSRA"
     gene            3723..4794
                     /gene="Srcap"
                     /label=Srcap
                     /note="Derived by automated computational analysis using 
                     gene prediction method: GNOMON. Supporting evidence 
                     includes similarity to: 4 mRNAs, 76 ESTs, 3 Proteins"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:100043597"
                     /db_xref="MGI:2444036"
     CDS             3723..3773
                     /codon_start=1
                     /gene="Srcap"
                     /product="helicase SRCAP"
                     /label=Srcap
                     /note="Derived by automated computational analysis using 
                     gene prediction method: GNOMON."
                     /note="color: #993366"
                     /db_xref="GI:309268993"
                     /db_xref="GeneID:100043597"
                     /db_xref="MGI:2444036"
                     /protein_id="XP_003084784.1"
                     /translation="QSSPSTAHPQLPILQTQ"
     primer_bind     complement(4866..4881)
                     /label=M13 rev (-24)
                     /label=M13\Reverse\Primer\(-24)
                     /note="color: #a6acb3; direction: LEFT"
     promoter        4875..4996
                     /label=Promoter_P_1 promoter
                     /label=Promoter_P_1
                     /note="lac promoter"
                     /note="color: #a6acb3"
     rep_origin      5216..5883
                     /label=Replication\origin
                     /note="pUC origin"
                     /note="color: #a6acb3"
     CDS             complement(5998..6891)
                     /label=bla
                     /note="color: #993366; direction: LEFT"
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gagtgagata gggcttctat tgtagcccag gctgtcctgg aactctttct
      721 ggagaccagg ctagcctcaa actaacagag atccacttac ctctgtctcc tgagtgctga
      781 ggttaaaggc atgtaccatg aatgcttagc atatttattc ttttgaaatg agatcttact
      841 ttgtgtttga ggctggtctc taatgcctat gttaaaacaa tcatctccaa tgtcccaaat
      901 agctgtgaat attggcatat acagatatca agatatgtct gtctaagcaa gttcttacag
      961 gtatttgata cagagtaagt atctaagaca tagggataga tagtatgttg agaaagggtc
     1021 tccgagtcaa tgcatagcca aagatgaact tgaacttctg atcttccttt acctctcaag
     1081 cttttggatt gaaggtgtgt gccaccctgc tggttttatg cagggttgag gattgaaccc
     1141 ttcttgggtg ctaggccgag ccagctgagc tacctccata tccctgaaaa tagttcttaa
     1201 ccccagcatt gggagctgag gcaggaggat tgtaagttcc aggccagtct ggatggtaga
     1261 ataagaccct aattgtctca aaaacaatta ttttaatagc ctttaatttt atttctactc
     1321 cttttctttt cctctctctc tctctctttc cttctttctt tctttctttc tttctttctt
     1381 tttttctttc tttctttctt tctttttatc caactgcagg tactccagag gctggtgaac
     1441 ctgagcggag gctgggacgc cctggtgggc cccgggccct ggaaggcagg tcccggtggc
     1501 tggtggccca gaatgaggcc agctcccagc atgccctgca gccggacacc agccactcgg
     1561 cctgcagcaa cggtgataac ccagtcattc ttcaggcatc cagcaaggag cctgggagtg
     1621 ggaccatggc caccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     1681 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     1741 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     1801 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     1861 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     1921 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     1981 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     2041 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     2101 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     2161 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     2221 gtgccagcgg tgaaggcaga ggctctctcc tcacatgtgg ggatgttgaa gaaaatccag
     2281 gtcccatgtc tagcgtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     2341 tcgagctgga cggcgacgta aacggccaca agttcagcgt gcgcggcgag ggcgagggcg
     2401 atgccaccaa cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     2461 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     2521 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     2581 gcaccatcac cttcaaggac gacggcacct acaagacccg cgccgaggtg aagttcgagg
     2641 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     2701 tcctggggca caagctggag tacaacttca acagccacaa cgtctatatc accgccgaca
     2761 agcagaagaa cggcatcaag gccaacttca agatccgcca caacgtggag gacggcagcg
     2821 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     2881 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg
     2941 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactcacggc atggacgagc
     3001 tgtacaaggg aggtaccggc agcgtggaac tgaacctgcg cgagacagag ctgtgcctgg
     3061 gactgcctgg cggcgataca gtggctcctg tgaccggcaa caagagaggc ttcagcgaga
     3121 cagtggacct gaagctgaac ctgaacaacg agcccgccaa caaagagggc agcaccaccc
     3181 acgacgtcgt gaccttcgac agcaaagaga agtccgcctg ccccaaggac cccgccaagc
     3241 ctcctgctaa agctcaggtc gtgggatggc ctcccgtgcg gagctacaga aagaacgtga
     3301 tggtgtcctg ccagaagtcc tctggcggcc ctgaggctgc cgccttcgtg aaagtgtcta
     3361 tggatggcgc cccttacctg agaaagatcg acctgagaat gtacaagagc tacgacgagc
     3421 tgagcaacgc cctgagcaac atgttcagca gcttcacaat gggcaagcac ggcggcgagg
     3481 aaggcatgat cgacttcatg aacgagcgga agctgatgga cctcgtgaac agctgggact
     3541 acgtgcccag ctacgaggac aaggacggcg actggatgct cgtgggagat gtgccctggc
     3601 ccatgttcgt ggacacatgc aagagactga gactgatgaa gggcagcgac gccatcggcc
     3661 tggcccccag agctatggaa aagtgcaaga gcagagccgg agggtctgga ggctctggat
     3721 cccagagcag cccctccact gctcatcctc agctcccaat cctacagaca caggtttgaa
     3781 agtggggaag gttcctatga ttatttctat gagctaattc atgcctttga aacaagtgat
     3841 gttgcaggtc ttcttaccca cctaagttta gactctcttt agttctcaga tagattgggg
     3901 agtaacatgt caagcctaat tttttcttct ttaatttggt tttctgagat aagagtttcc
     3961 ccacctactg tcttggaact tgctctgtac accaggctgg ccttgaactc agatccattt
     4021 gtctctgact tcagatactt aacttctact taacataata atgccgattt ccaagagtac
     4081 atatcttgaa aggtgtgctg gcgcgtgcct ttagcgtgcc tttagtcaca gcactcaggc
     4141 agaggcaggc agatctctat gagataaggc cagtaaacac tatatagtga gagcctgtct
     4201 tttttttttt tttttttttt tttggttttt cgagacaggg tttctctgtg tagccctggc
     4261 tgttctgaaa ctcactttgt agactaggct ggcctcgaac tcagaaatcc gcctgcctct
     4321 gcctcccaag tgctgggatt aaaggcgtga gccaacactg cctagcctga gatcctatct
     4381 ttaaaaaaaa gacacatgga ctggtgagat ggctcagtgg tttagagcac tcatggctct
     4441 tccagagttc aattcccagc aaccacatgg tggctcacaa ccatctgtaa taggattcag
     4501 tgacctcttc tgatatgtct gaagagagca cagtgtactc acatacataa aaatcttcaa
     4561 aaaattatta aagaaaagaa aaaagacaca ggtttttctt accataagga ggtctaggct
     4621 aagccagaag gtggtggtgc atgcctttaa tcccagtatt taggaggcag aggcagttca
     4681 aggccagctt ggactacaga gcaagttcta gacagccaag gctacccaga gacaccctga
     4741 cgtaaaataa tgaaaaacga aagaaagaga ggtctaggtt aggccgtaaa tggagcggcc
     4801 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattt cgagcttggc
     4861 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4921 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4981 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5041 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5101 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5161 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5221 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5281 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5341 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5401 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5461 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5521 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5581 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5641 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5701 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5761 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5821 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5881 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5941 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6001 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6061 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6121 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6181 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6241 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6301 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6361 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6421 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6481 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6541 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6601 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6661 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6721 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6781 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6841 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6901 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6961 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgc
//
