LOCUS       Exported                5815 bp ds-DNA     circular SYN 29-JUN-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 22, 2019 from SnapGene 4.3.8.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..1719
                     /label=CAG\promoter
                     /note="color: #aaaaaa"
     primer_bind     257..276
                     /label=LJT234
                     /note="color: black; sequence: GCCAAGTACGCCCCCTATTG"
     primer_bind     727..746
                     /label=LJT100
                     /note="color: black; sequence: ctgactgaccgcgttactcc"
     primer_bind     783..810
                     /label=LJT185
                     /note="color: black; sequence: 
                     gctgtaattagcgcttggtttaatgacg"
     primer_bind     complement(794..815)
                     /label=LJT186
                     /note="color: black; sequence: cgagccgtcattaaaccaagcg"
     CDS             1731..2753
                     /codon_start=1
                     /label=SB100X\CDS
                     /note="color: #993366"
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    2763..3305
                     /label=rabbit\globin\poly\A
                     /note="color: #aaaaaa"
     primer_bind     complement(3361..3383)
                     /label=LJT194
                     /note="color: black; sequence: cttccggctcgtatgttgtgtgg"
     primer_bind     complement(3390..3408)
                     /label=LJT181
                     /note="color: black; sequence: GGCACCCCAGGCTTTACAC"
     primer_bind     complement(3573..3591)
                     /label=LJT333
                     /note="color: black; sequence: ttagtcagccatggggcgg"
     primer_bind     3573..3591
                     /label=LJT332
                     /note="color: black; sequence: ccgccccatggctgactaa"
     primer_bind     complement(4105..4124)
                     /label=LJT183
                     /note="color: black; sequence: cacctctgacttgagcgtcg"
     primer_bind     4915..4934
                     /label=LJT245
                     /note="color: black; sequence: acgatacgggagggcttacc"
     primer_bind     4916..4937
                     /label=LJT068
                     /note="color: black; sequence: cgatacgggagggcttaccatc"
     primer_bind     complement(4919..4940)
                     /label=LJT067
                     /note="color: black; sequence: ccagatggtaagccctcccgta"
     primer_bind     complement(4946..4965)
                     /label=LJT129
                     /note="color: black; sequence: tcgcggtatcattgcagcac"
     primer_bind     5719..5741
                     /label=LJT151
                     /note="color: black; sequence: CAGGGTTATTGTCTCATGAGCGG"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgccacc atgggaaaat
     1741 caaaagaaat cagccaagac ctcagaaaaa gaattgtaga cctccacaag tctggttcat
     1801 ccttgggagc aatttccaaa cgcctggcgg taccacgttc atctgtacaa acaatagtac
     1861 gcaagtataa acaccatggg accacgcagc cgtcataccg ctcaggaagg agacgcgttc
     1921 tgtctcctag agatgaacgt actttggtgc gaaaagtgca aatcaatccc agaacaacag
     1981 caaaggacct tgtgaagatg ctggaggaaa caggtacaaa agtatctata tccacagtaa
     2041 aacgagtcct atatcgacat aacctgaaag gccactcagc aaggaagaag ccactgctcc
     2101 aaaaccgaca taagaaagcc agactacggt ttgcaactgc acatggggac aaagatcgta
     2161 ctttttggag aaatgtcctc tggtctgatg aaacaaaaat agaactgttt ggccataatg
     2221 accatcgtta tgtttggagg aagaaggggg aggcttgcaa gccgaagaac accatcccaa
     2281 ccgtgaagca cgggggtggc agcatcatgt tgtgggggtg ctttgctgca ggagggactg
     2341 gtgcacttca caaaatagat ggcatcatgg acgccgtgca gtatgtggat atattgaagc
     2401 aacatctcaa gacatcagtc aggaagttaa agcttggtcg caaatgggtc ttccaacacg
     2461 acaatgaccc caagcatact tccaaagttg tggcaaaatg gcttaaggac aacaaagtca
     2521 aggtattgga gtggccatca caaagccctg acctcaatcc tatagaaaat ttgtgggcag
     2581 aactgaaaaa gcgtgtgcga gcaaggaggc ctacaaacct gactcagtta caccagctct
     2641 gtcaggagga atgggccaaa attcacccaa attattgtgg gaagcttgtg gaaggctacc
     2701 cgaaacgttt gacccaagtt aaacaattta aaggcaatgc taccaaatac taggcggccg
     2761 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     2821 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     2881 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     2941 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     3001 gaatgagtat ttggtttaga gtttggcaac atatgccata tgctggctgc catgaacaaa
     3061 ggtggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     3121 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     3181 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     3241 gactactccc agtcatagct gtccctcttc tcttatgaag atccctcgac ctgcagccca
     3301 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     3361 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     3421 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     3481 cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     3541 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3601 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     3661 tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta
     3721 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3781 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga
     3841 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     3901 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3961 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4021 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4081 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4141 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4201 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     4261 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4321 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4381 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4441 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4501 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4561 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4621 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4681 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4741 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4801 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4861 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4921 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4981 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     5041 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     5101 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     5161 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5221 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5281 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5341 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5401 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5461 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5521 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5581 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5641 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5701 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5761 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctg
//
