LOCUS       Exported                8094 bp ds-DNA     circular SYN 12-JUN-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Groudine Lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, May 22, 2019 from SnapGene 4.3.8.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     49..65
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        74..94
                     /label=T7 promoter
                     /label=T7
                     /note="color: #a6acb3"
     promoter        75..93
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     modified_base   115..118
                     /label=TATA
                     /note="color: #a6acb3"
     CDS             complement(119..345)
                     /label=IR/DR(L)\Lmut44
                     /note="IR/DR(L) appears to have a spontaneous mutation from
                     A to T at bp 44 (happens to revert back to consensus) [the 
                     bp designation corresponds to the original defined IR/DR(L)
                     from Lo to Li, not the plasmid]"
                     /note="color: #993366; direction: LEFT"
     primer_bind     complement(380..421)
                     /label=LJT525
                     /note="color: black; sequence: 
                     accggagcggatctagcttctaaagccatgacatcattttct"
     primer_bind     complement(396..421)
                     /label=LJT528
                     /note="color: black; sequence: accggagcggatctagcttctaaagc"
     primer_bind     407..429
                     /label=LJT526
                     /note="color: black; sequence: tagatccgctccggtgcccgtca"
     promoter        414..1595
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     intron          644..1586
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     primer_bind     complement(1557..1603)
                     /label=LJT527
                     /note="color: black; sequence: 
                     gcgtcgagtcacgacacctgaaatggaagaaaaaaactttgaaccac"
     primer_bind     complement(1575..1603)
                     /label=LJT529
                     /note="color: black; sequence: 
                     gcgtcgagtcacgacacctgaaatggaag"
     primer_bind     1589..1613
                     /label=LJT524
                     /note="color: black; sequence: gtcgtgactcgacgccaccatgACG"
     primer_bind     complement(1596..1617)
                     /label=LJT327
                     /note="color: black; sequence: AGTACGTCATggtggcgtcgag"
     primer_bind     1598..1619
                     /label=LJT328
                     /note="color: black; sequence: cgacgccaccATGACGTACTTC"
     CDS             1608..3332
                     /codon_start=1
                     /label=OsTIR
                     /note="color: #993366"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     1640..1659
                     /label=LJT163
                     /note="color: black; sequence: CATCTTCAGCTTCCTGCCGG"
     primer_bind     1673..1691
                     /label=LJT215
                     /note="color: black; sequence: CAACACGGTCTCGCTCGTC"
     primer_bind     1698..1719
                     /label=LJT164
                     /note="color: black; sequence: GTGTGGTACGAGATCGAGAGGC"
     primer_bind     complement(1803..1822)
                     /label=LJT216
                     /note="color: black; sequence: GGCTTCCCCTTCACCGTGAG"
     primer_bind     1824..1843
                     /label=LJT211
                     /note="color: black; sequence: CACTTCGCCGACTTCAACCT"
     primer_bind     complement(1938..1956)
                     /label=LJT212
                     /note="color: black; sequence: GGCTCTCGTCGGACACCAC"
     primer_bind     complement(1988..2010)
                     /label=LJT223
                     /note="color: black; sequence: AGCTGATAAGAACAAGAGCCCTG"
     primer_bind     complement(2190..2211)
                     /label=LJT165
                     /note="color: black; sequence: CAAGTCTCTCCAGTGAACCAGC"
     primer_bind     2303..2323
                     /label=LJT166
                     /note="color: black; sequence: GGAGGATTTGGGGACAGGGAA"
     primer_bind     2813..2832
                     /label=LJT320
                     /note="color: black; sequence: TGAGCCAGGGAAGCCTGATG"
     primer_bind     3065..3086
                     /label=LJT330
                     /note="color: black; sequence: CCCGTTTGGTGATGCTGCACTC"
     primer_bind     3170..3189
                     /label=LJT319
                     /note="color: black; sequence: TGCGTCAAAGATGCCGATGC"
     primer_bind     complement(3297..3317)
                     /label=LJT331
                     /note="color: black; sequence: ATTTGGTGCATCATCCCTCGC"
     misc_feature    3362..3958
                     /label=IRES
                     /note="color: #aaaaaa; direction: RIGHT"
     primer_bind     complement(3632..3651)
                     /label=LJT338
                     /note="color: black; sequence: cctgcaaagggtcgctacag"
     primer_bind     3710..3729
                     /label=LJT337
                     /note="color: black; sequence: atacacctgcaaaggcggca"
     CDS             3959..4759
                     /codon_start=1
                     /label=NEO
                     /note="color: #aaaaaa"
                     /translation="MMGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3962..4759
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3977..3997)
                     /label=LJT321
                     /note="color: black; sequence: agaacctgcgtgcaatccatc"
     primer_bind     complement(4130..4150)
                     /label=LJT336
                     /note="color: black; sequence: gtcctgcagttcattcagggc"
     primer_bind     4130..4150
                     /label=LJT334
                     /note="color: black; sequence: GCCCTGAATGAACTGCAGGAC"
     primer_bind     4357..4375
                     /label=LJT237
                     /note="color: black; sequence: CCCATTCGACCACCAAGCG"
     primer_bind     4652..4671
                     /label=LJT335
                     /note="color: black; sequence: GAAGAGCTTGGCGGCGAATG"
     polyA_signal    4784..5011
                     /label=BGH\polyA
                     /note="color: #aaaaaa"
     primer_bind     complement(4795..4812)
                     /label=LJT063
                     /note="color: black; sequence: tagaaggcacagtcgagg"
     polyA_signal    4801..5008
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(4953..4970)
                     /label=LJT101
                     /note="color: black; sequence: cttgctgtcctgccccac"
     CDS             5084..5311
                     /label=IR/DR(R)\Rmut13,6130,143,150
                     /note="IR/DR(R) has PCR induced mutations at bps 13 (T to A
                     in the Ri), deleting bp 130 (6T), 143 (C to T) and 150 (C 
                     to A) [these bp designations correspond to the original 
                     defined IR/DR(R) from Ri to Ro, not the plasmid]"
                     /note="color: #993366; direction: RIGHT"
     modified_base   5312..5315
                     /label=TATA
                     /note="color: #a6acb3"
     primer_bind     complement(5352..5371)
                     /label=LJT008
                     /note="color: black; sequence: ttaaccctcactaaagggaa"
     promoter        complement(5354..5373)
                     /label=T3 promoter
                     /label=T3
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(5355..5373)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(5394..5410)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     promoter        complement(5398..5520)
                     /label=LacZ\promoter promoter
                     /label=LacZ\promoter
                     /note="color: #a6acb3; direction: LEFT"
     protein_bind    5418..5434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     complement(5437..5459)
                     /label=LJT194
                     /note="color: black; sequence: cttccggctcgtatgttgtgtgg"
     promoter        complement(5442..5472)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 5442 .. 5448 / #ffffff / -10
                        2: 5449 .. 5466 / #ffffff
                        3: 5467 .. 5472 / #ffffff / -35"
     primer_bind     complement(5466..5484)
                     /label=LJT181
                     /note="color: black; sequence: GGCACCCCAGGCTTTACAC"
     protein_bind    5487..5508
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      5614..6494
                     /label=ColE\Ori
                     /note="color: #a6acb3"
     rep_origin      complement(5796..6384)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(5836..5855)
                     /label=LJT183
                     /note="color: black; sequence: cacctctgacttgagcgtcg"
     CDS             complement(6555..7415)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6555 .. 7346 / #ccffcc
                        2: 7347 .. 7415 / #ccffcc / signal sequence
                     Cleavage site after base 7346"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(6558..7415)
                     /label=Amp
                     /note="color: #993366; direction: LEFT"
     primer_bind     6646..6665
                     /label=LJT245
                     /note="color: black; sequence: acgatacgggagggcttacc"
     primer_bind     6647..6668
                     /label=LJT068
                     /note="color: black; sequence: cgatacgggagggcttaccatc"
     primer_bind     complement(6650..6671)
                     /label=LJT067
                     /note="color: black; sequence: ccagatggtaagccctcccgta"
     primer_bind     complement(6677..6696)
                     /label=LJT129
                     /note="color: black; sequence: tcgcggtatcattgcagcac"
     promoter        complement(7416..7520)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     7450..7472
                     /label=LJT151
                     /note="color: black; sequence: CAGGGTTATTGTCTCATGAGCGG"
     rep_origin      7546..8002
                     /label=F1\Ori
                     /note="color: #a6acb3"
     rep_origin      7547..8002
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     8013..8032
                     /label=LJT131
                     /note="color: black; sequence: ttcaggctgcgcaactgttg"
     primer_bind     join(8090..8094,1..17)
                     /label=LJT130
                     /note="color: black; sequence: tgctgcaaggcgattaagttgg"
ORIGIN
        1 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
       61 ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctcgga tccctataca
      121 gttgaagtcg gaagtttaca tacacttaag ttggagtcat taaaactcgt ttttcaacta
      181 ctccacaaat ttcttgttaa caaacaatag ttttggcaag tcagttagga catctacttt
      241 gtgcatgaca caagtcattt ttccaacaat tgtttacaga cagattattt cacttataat
      301 tcactgtatc acaattccag tgggtcagaa gtttacatac actaagttga ctgtgccttt
      361 aaacagcttg gaaaattcca gaaaatgatg tcatggcttt agaagctaga tccgctccgg
      421 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      481 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      541 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      601 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      661 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      721 gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
      781 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
      841 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
      901 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
      961 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     1021 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     1081 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     1141 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     1201 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     1261 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     1321 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     1381 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     1441 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     1501 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     1561 ttcaaagttt ttttcttcca tttcaggtgt cgtgactcga cgccaccatg acgtacttcc
     1621 cggaggaggt ggtggagcac atcttcagct tcctgccggc gcagcgcgac cgcaacacgg
     1681 tctcgctcgt ctgcaaggtg tggtacgaga tcgagaggct gagccgccgc ggcgtcttcg
     1741 tgggcaactg ctacgccgtg cgcgccggcc gcgtcgccgc gcggttcccc aacgtgcggg
     1801 cgctcacggt gaaggggaag ccccacttcg ccgacttcaa cctcgtgccc cccgactggg
     1861 gcggctacgc ggggccgtgg atcgaggcgg ccgcgagggg atgccacggc ctggaggagc
     1921 tcaggatgaa gcggatggtg gtgtccgacg agagcctcga gctgctggct cgctcgttcc
     1981 cgcggttcag ggctcttgtt cttatcagct gcgaggggtt cagcactgac gggctagccg
     2041 ccgtcgcgag ccattgcaag cttctgaggg agttggattt gcaggaaaat gaagtggagg
     2101 atcgagggcc taggtggctt tcctgcttcc ctgattcctg cacatcactt gtctcattga
     2161 attttgcctg catcaaaggg gaggttaatg ctggttcact ggagagactt gttagcaggt
     2221 ccccaaacct gcggagtttg aggctgaatc gatctgtatc ggtagataca cttgcaaaga
     2281 tactactgcg tacccctaac ttggaggatt tggggacagg gaatttgaca gatgacttcc
     2341 aaactgagtc ctactttaag cttaccagtg ctctggagaa atgcaagatg ttgaggagtt
     2401 tgtctggatt ctgggatgct tctcctgttt gcctgtcatt tatctacccc ctgtgtgctc
     2461 aactgacagg attgaacttg agctatgccc ccacacttga tgcttctgac cttacaaaaa
     2521 tgattagccg ctgtgtgaag ctccaacgcc tttgggtact ggattgtatc tcggacaaag
     2581 gcttgcaagt ggtggcctcc agttgcaaag acttgcaaga actcagggta tttccatcag
     2641 atttctacgt agctggttat tctgcagtga cagaggaggg acttgttgca gtatccttgg
     2701 gctgtccaaa actgaactca ctactgtact tctgtcacca aatgactaat gctgcactag
     2761 ttactgtcgc caagaactgt ccaaatttca cacgattcag actttgtatt cttgagccag
     2821 ggaagcctga tgttgtgaca agccaaccat tagatgaagg ctttggagct attgttcgtg
     2881 agtgcaaggg attacaacgt ttgtcaatat ctggtcttct cacagacaaa gttttcatgt
     2941 atattgggaa atatgcaaaa caacttgaga tgctttctat agcatttgct ggtgacagtg
     3001 ataagggtat gatgcatgtt atgaatggat gcaagaattt aaggaaactg gagataagag
     3061 atagcccgtt tggtgatgct gcactcttgg ggaattttgc taggtacgag acaatgcgat
     3121 ccctttggat gtcatcttgc aatgtcacgt taaaggggtg ccaagtcctt gcgtcaaaga
     3181 tgccgatgct caatgttgag gtcataaatg agcgggatgg tagcaatgaa atggaggaaa
     3241 accatggaga tctgcctaaa gtggagaaat tatatgtgta ccgcacaact gctggggcga
     3301 gggatgatgc accaaatttt gttaaaatcc tatgatctag caagtcgaca ctagcaagtc
     3361 gggctgcatc tagggcggcc aattccgccc ctctcccccc cacccaacgt tactggccga
     3421 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     3481 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     3541 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     3601 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     3661 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     3721 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     3781 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     3841 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     3901 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgccaccat
     3961 gatgggaatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4021 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     4081 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     4141 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     4201 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     4261 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     4321 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     4381 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     4441 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     4501 cgacggcgac gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     4561 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     4621 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     4681 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     4741 tcttgacgag ttcttctgac tcgacaaggc cgcctcggcc agctcgctga tcagcctcga
     4801 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     4861 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     4921 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     4981 gggaagacaa tagcaggcat gctggggatg gaagcttgtg gaaggctact cgaaatgttt
     5041 gacccaagtt aaacaattta aaggcaatgc taccaaatac taattgagtg tatgtaaact
     5101 tctgacccac tgggaatgtg atgaaagaaa taaaagctga aatgaatcat tctctctact
     5161 attattctga tatttcacat tcttaaaata aagtggtgat cctaactgac ctaagacagg
     5221 gaatttttac taggattaaa tgtcaggaat tgtgaaaaag tgagtttaaa tgtatttggc
     5281 taaggtgtat gtaaacttcc gacttcaact gtatagggat cctctagcta gagtcgatcg
     5341 accagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
     5401 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     5461 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     5521 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     5581 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5641 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5701 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5761 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5821 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5881 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5941 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6001 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6061 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6121 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6181 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     6241 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6301 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6361 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6421 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6481 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6541 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6601 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6661 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6721 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6781 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6841 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6901 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6961 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7021 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7081 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7141 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7201 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7261 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7321 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7381 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7441 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7501 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc
     7561 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     7621 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     7681 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7741 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     7801 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7861 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     7921 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     7981 aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc
     8041 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctg
//
