LOCUS       pAcGFP-C1               4760 bp ss-DNA     circular SYN 28-Sep-2011
DEFINITION  .
FEATURES             Location/Qualifiers
     regulatory      1..590
                     /note="CMV promoter"
     frag            592..1311
                     /date="02-11#-2016"
                     /left_cutter="NheI (7)"
                     /left_end="5CTAG"
                     /note="8 to 727 of mNeonGreenHsOpt-NheIORFBepEISpeI"
                     /right_cutter="BspEI (727)"
                     /right_end="5CCGG"
     frag            <592..602
                     /date="29-9#-2016"
                     /note=" [Split]"
     CDS             592..1311
                     /note="Humanized mNeonGreen"
     frag            597..>602
                     /date="26-8#-2016"
                     /note="619 to 649 of pCRY2(#NLS)-mCherryN1"
     frag            603..>1310
                     /date="29-9#-2016"
                     /note="1 to 711 of mNeonGreenHsOpt"
     frag            1311
                     /date="29-9#-2016"
                     /note="2462 to 2478 of CIB1-wos-BspEI-Spe-Rv [Split]"
     frag            <1312..1373
                     /note="1237 to 2037 of AcGFP-G_ORF"
     frag            <1312..>1373
                     /note="1237 to 2081 of pUB-Pur-AcGFP-G [Split]"
     CDS             <1312..>1320
                     /gene="AcGFP-[GGGS]x3"
                     /note=""
     frag            <1369..>1373
                     /note="2 to 800 of AcGFP-C1_ORF.seq [Split]"
     CDS             1369..1431
                     /note="MCS"
BASE COUNT  1180 A  1202 C  1238 G  1140 T  0 OTHER
ORIGIN
        1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG
       61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
      121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA
      181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
      241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA
      301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
      361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG
      421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG
      481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT
      541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCCA
      601 CCATGGTCAG CAAAGGCGAA GAAGACAACA TGGCATCACT GCCAGCGACA CACGAACTTC
      661 ATATTTTCGG CTCCATCAAT GGTGTGGACT TCGATATGGT TGGACAGGGT ACTGGGAACC
      721 CTAACGATGG ATATGAGGAG TTGAACTTGA AGAGTACCAA GGGCGATCTT CAGTTTTCAC
      781 CTTGGATTCT CGTACCTCAT ATAGGTTATG GCTTTCATCA ATATCTGCCC TATCCTGACG
      841 GTATGTCACC TTTCCAAGCA GCTATGGTTG ACGGGAGCGG TTATCAGGTT CACCGGACAA
      901 TGCAGTTTGA AGATGGAGCC TCTTTGACCG TAAATTATCG GTATACTTAT GAGGGCAGCC
      961 ATATTAAAGG CGAGGCTCAA GTTAAGGGGA CTGGGTTCCC CGCGGATGGT CCCGTAATGA
     1021 CAAACAGTCT GACGGCTGCA GACTGGTGCC GCTCTAAGAA GACATATCCA AATGACAAGA
     1081 CGATTATTAG CACTTTCAAG TGGTCATACA CTACAGGAAA TGGTAAACGC TATAGGAGCA
     1141 CTGCCCGGAC CACGTATACT TTCGCGAAGC CGATGGCGGC AAATTATTTG AAAAACCAAC
     1201 CGATGTATGT GTTCAGAAAG ACAGAACTCA AGCATTCTAA AACTGAACTG AACTTCAAAG
     1261 AGTGGCAGAA AGCCTTTACT GATGTAATGG GCATGGACGA ACTCTATAAG TCCGGACTCA
     1321 CCGGTGGAGG CGGTTCAGGC GGAGGTGGCT CTGGCGGTGG CGGATCGAGA TCTCGAGCTC
     1381 AAGCTTCGAA TTCTGCAGTC GACGGTACCG CGGGCCCGGG ATCCACCGGA TCTAGATAAC
     1441 TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC
     1501 ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG
     1561 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT
     1621 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA CGCGTAAATT
     1681 GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT
     1741 AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG
     1801 TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC
     1861 AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA
     1921 AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA
     1981 TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA
     2041 GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC
     2101 GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC
     2161 GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA
     2221 TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTCCTGAGGC GGAAAGAACC
     2281 AGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA
     2341 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC
     2401 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC
     2461 TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT
     2521 GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA
     2581 AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AGATCGATCA AGAGACAGGA
     2641 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG
     2701 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC
     2761 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT
     2821 GCCCTGAATG AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT
     2881 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC
     2941 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC
     3001 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC
     3061 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG
     3121 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG
     3181 GCGAGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT
     3241 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG
     3301 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA
     3361 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC
     3421 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC
     3481 AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT
     3541 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA
     3601 TGCTGGAGTT CTTCGCCCAC CCTAGGGGGA GGCTAACTGA AACACGGAAG GAGACAATAC
     3661 CGGAAGGAAC CCGCGCTATG ACGGCAATAA AAAGACAGAA TAAAACGCAC GGTGTTGGGT
     3721 CGTTTGTTCA TAAACGCGGG GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT ACCCCACCGA
     3781 GACCCCATTG GGGCCAATAC GCCCGCGTTT CTTCCTTTTC CCCACCCCAC CCCCCAAGTT
     3841 CGGGTGAAGG CCCAGGGCTC GCAGCCAACG TCGGGGCGGC AGGCCCTGCC ATAGCCTCAG
     3901 GTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG
     3961 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT
     4021 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG
     4081 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC
     4141 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA
     4201 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA
     4261 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC
     4321 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG
     4381 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC
     4441 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG
     4501 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT
     4561 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT
     4621 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
     4681 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA
     4741 ACCGTATTAC CGCCATGCAT
//
