LOCUS       pXT184_addgene          7968 bp ds-DNA     circular     29-MAY-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    5665..5894
                     /label="sgRNA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             110..121
                     /label="RBS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      5545..5604
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             1886..2716
                     /label="ShTnsC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             2713..3216
                     /label="ShTniQ"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     gene            128..1882
                     /label="ShTnsB"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    5630..5664
                     /label="J23119"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    6226..6330
                     /label="ampR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      7286..7968
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             6529..7188
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5919..5946
                     /label="terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        79..109
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    5895..5918
                     /label="LguI golden gate sites"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             3607..5526
                     /label="ShCas12jk"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tttttacggt tcctggcctt ttgctggcct tttgcACGCC AAGCTCCAAA ACGATCTCAA
       61 GAAGATCATC TTATTAAttt tacactttat gcttccggct cgtatgttga aagaggagaa
      121 aggatctatg aacagtcagc aaaatcctga tttagctgtt catcccttgg caattcctat
      181 ggaaggctta ctaggagaaa gtgctacaac tcttgagaag aatgtaattg ccacacaact
      241 ctcagaggaa gcccaagtaa agctagaggt aatccaaagt ttactggaac cctgcgatcg
      301 cacaacttat gggcaaaagt tgcgggaagc agcagagaaa ctaaatgtat cgttgcgaac
      361 ggtacaaagg ttggtgaaaa actgggaaca agatggctta gtcggactca ctcaaacaag
      421 tagggctgat aaaggaaaac accgcattgg tgagttttgg gaaaacttca ttaccaaaac
      481 ctacaaggag ggtaacaagg gaagtaaacg tatgacccct aaacaagttg ctctcagagt
      541 cgaggctaaa gcccgtgaat taaaagactc taagccgccc aattacaaaa ccgtgttacg
      601 ggtattagca cccattttgg aaaagcaaca aaaagccaag agtatccgca gtcctggttg
      661 gagaggaact acgctttcgg ttaaaacccg tgaaggaaaa gatttatcgg ttgattacag
      721 taaccatgtt tggcaatgtg accatacccg cgtggatgtg ttgctggtag atcaacatgg
      781 tgaaatttta agtcgtccct ggctaacaac agtaattgat acttactctc gttgcattat
      841 gggtatcaac ttgggctttg atgcacccag ttctggggta gtagcattag cgttacgcca
      901 tgcaattcta ccaaagcgtt acggttccga gtacaaactg cattgtgagt ggggaaccta
      961 tggaaaacca gaacattttt atactgatgg cggtaaagac tttcgctcta accacttgag
     1021 tcagattggg gcgcaattgg gatttgtctg tcatttacgc gatcgccctt ctgaaggtgg
     1081 agtagtagaa cgtcccttca aaacattaaa tgaccaacta ttttcaacgc ttcctgggta
     1141 caccggatct aatgtgcagg aacgcccaga agatgcagag aaggacgcaa gacttacttt
     1201 gcgagaacta gaacagttac ttgtgcgtta catcgtagat cgttacaacc aaagtattga
     1261 tgcgcggatg ggcgaccaaa cgcgctttga gcgttgggaa gcaggattgc ctacagtgcc
     1321 agtaccaata ccagaacgag atttggatat ttgtttaatg aagcagtcac ggcgcactgt
     1381 gcaaagaggt ggttgtttgc agtttcagaa tttaatgtat cggggggaat atttggcagg
     1441 ttatgccgga gaaactgtca acttaaggtt tgaccccaga gacattacaa caattttggt
     1501 ttatcgccag gaaaacaatc aggaagtatt tctgactcgc gctcacgctc aaggtttgga
     1561 gacagagcaa ctggcattag atgaggctga ggcagcaagt cgcagactcc gtaccgcagg
     1621 gaaaactatc agtaaccaat cattattgca agaagttgtt gaccgcgatg ctcttgtcgc
     1681 taccaagaaa agccgtaagg agcgtcaaaa attggaacag actgttttgc gatctgctgc
     1741 tgttgatgaa agtaatagag aatccttgcc ttctcaaata gttgaaccag atgaagtgga
     1801 atctacagaa acggttcact ctcaatacga agacattgag gtgtgggact atgaacaact
     1861 tcgtgaagaa tatgggtttt aaacaatgac agaagctcag gcgatcgcca agcagttggg
     1921 tggggtaaaa ccggatgatg agtggttaca agctgaaatt gctcgtctca agggtaagag
     1981 cattgtgcct ttacagcagg taaaaactct ccatgattgg ttagatggca agcgcaaggc
     2041 aagaaaatct tgccgagtag ttggggaatc gagaactggc aagacagttg cttgtgatgc
     2101 ctacagatac aggcacaaac ctcagcagga agctggacga cctccaactg tgcctgtcgt
     2161 ttatattcga cctcaccaaa aatgtggccc caaggatttg tttaaaaaga ttactgagta
     2221 cctcaagtat cgggtaacaa aagggactgt atctgatttt cgagatagga cgatagaagt
     2281 actcaagggt tgtggcgtag agatgctaat tattgatgaa gctgaccgtc tcaagcctga
     2341 aacttttgct gatgtgcgag atattgccga agatttagga attgctgtgg tactggtagg
     2401 aacagaccgt ttggatgcgg taattaagcg ggatgagcag gttctcgaac gctttcgggc
     2461 gcatcttcgc tttggtaaat tgtcgggaga ggattttaag aacaccgtag aaatgtggga
     2521 acaaatggtt ttgaaactgc cagtatcttc taatctaaag agcaaggaga tgctacggat
     2581 tctcacgtca gcaactgaag gctacattgg tcgccttgat gagattctta gggaagctgc
     2641 aattcgttcc ttatcaagag gattgaagaa gattgacaag gctgttttac aggaagtagc
     2701 taaggagtac aaatgataga agcaccagat gttaaacctt ggctattctt gattaaaccc
     2761 tatgaagggg aaagcctgag ccactttctt ggcaggttca gacgtgccaa ccatttatcc
     2821 gcaagtggat tgggtacttt ggcaggaatt ggtgctatag tggcacgttg ggaaagattt
     2881 cattttaatc ctcgccctag tcagcaagaa ttggaagcga tcgcatctgt agtagaagtg
     2941 gatgctcaaa ggttagccca gatgttaccg cctgctggag tgggaatgca gcatgagcca
     3001 attcgcttgt gtggggcttg ttatgccgag tcgccttgtc accgaattga atggcagtac
     3061 aagtcggtgt ggaagtgcga tcgccatcaa ctcaagattt tagcaaagtg tccaaactgt
     3121 caagcacctt ttaaaatgcc tgcgctgtgg gaggatgggt gctgtcacag atgtaggatg
     3181 ccgtttgcag aaatggcaaa gctacagaag gtttgaCTGC AGtgataaaa ccagaaaaag
     3241 gtgtgaaatt aactaagtcc ctgaattgat ctggttgtcc aaaaaatttg tgcgatcgca
     3301 tggcaagatt attcctacta ttgatgtggc gtgagtcgga taacttgctc taatgGGATC
     3361 CCGGTTAATA CCAATAGATC AGATATGAGT AATAAACATT ACGACCTTTA CCTGTGGTAT
     3421 TCACTGTTGG CACGACAACA CCTTTATCGC GCCAATACTG TAGCTGACGA CGCGAACAAT
     3481 TAGTGATTTG AGCCGCTTCT GTGCTTGTGT AGAATTTTCC TTCCATAGAA ATCATCCTAC
     3541 AGAAGTAGTT TTTTAAATTT ACATTTTTTA AAAGCACATT ATGTATTCAT GTGATATTAT
     3601 TTTTTtatga gtcaaataac tattcaagct cgacttattt cctttgaatc aaaccgccaa
     3661 caactctgga agttgatggc agatttaaac acgccgttaa ttaacgaact gctttgccag
     3721 ttaggtcaac accccgactt cgagaagtgg caacaaaagg gtaaactccc gtctaccgtt
     3781 gtgagccagt tatgtcaacc tctcaaaact gaccctcgct ttgcaggtca gcccagccgt
     3841 ttatatatgt cggcaattca tattgtggac tacatctaca agtcctggct ggctatacag
     3901 aaacggcttc aacagcagct agatggaaag acgcgctggc tagaaatgct caatagcgat
     3961 gctgaattag tagaacttag tggtgacact ttagaggcta ttcgtgtcaa agctgctgaa
     4021 attttggcaa tagctatgcc agcatctgag tcagatagcg cttcacctaa agggaaaaaa
     4081 ggtaaaaagg agaaaaaacc ctcatcttct agccctaagc gtagtttatc caagacatta
     4141 tttgacgctt accaagaaac ggaagatatc aagagccgta gcgccatcag ctacctgtta
     4201 aaaaatggct gcaaacttac tgacaaagaa gaagattcag aaaaatttgc taaacgtcgt
     4261 cgtcaagttg aaatccaaat tcaaaggctt accgaaaagt taataagtcg gatgcctaaa
     4321 ggtcgagatt tgaccaatgc taaatggttg gagacactct tgactgctac aaccactgtt
     4381 gctgaagaca acgcccaagc caaacgctgg caggatattc tgttaactcg atcaagttct
     4441 ctcccattcc cccttgtttt tgaaaccaac gaggatatgg tttggtcaaa gaatcaaaag
     4501 ggtaggctgt gtgttcactt caatggctta agcgatttaa tttttgaggt gtactgcggc
     4561 aatcgtcaac ttcactggtt tcaacgcttc ctagaagacc aacagactaa acgcaaaagc
     4621 aaaaatcagc attctagcgg cttgttcaca ctcagaaatg gtcatctagt ttggcttgaa
     4681 ggtgagggta aaggggaacc ttggaatctt caccacttga ccctttactg ctgtgttgac
     4741 aatcgcttgt ggacagagga gggaacagaa atcgttcgcc aagagaaagc agatgaaatt
     4801 actaaattca tcacaaacat gaagaagaaa agcgatctaa gcgatacaca gcaagctttg
     4861 attcaacgta aacaatcaac acttactcga ataaacaatt cctttgagcg tcctagccaa
     4921 cccctttatc aaggtcaatc acacattttg gttggagtaa gcctgggact agaaaaacct
     4981 gccacagtag cagtagtaga tgcgatcgcc aacaaagtct tggcttaccg gagtattaaa
     5041 caattacttg gcgacaatta cgaactgcta aatcgccaga gacgacaaca gcagtaccta
     5101 tctcacgaac gccacaaagc acaaaaaaac ttctctccca atcaatttgg agcatctgag
     5161 ttagggcaac atatagacag attattagct aaagcaattg tagcgttagc gagaacctac
     5221 aaagctggca gtattgtctt gcccaagtta ggggatatgc gggaggttgt ccaaagtgaa
     5281 attcaagcta tagcagaaca aaaatttccc ggttatattg aaggtcagca aaaatatgcc
     5341 aaacagtacc gggttaatgt tcatcggtgg agctacggca gattaattca aagcattcaa
     5401 agtaaagcag ctcaaacagg aattgtgatt gaggagggaa aacaacctat tcgaggtagt
     5461 ccccacgaca aagcaaagga attagcactt tctgcttaca atctccgcct aactaggcga
     5521 agttaaGTAA GGATCTCCAG GCATCAAATA AAACGAaagg ctcagtcgaa agactgggcc
     5581 tttcgtttta tctgttgttt gtcggtgaac gctctctact agaGAGACCC tgacagctag
     5641 ctcagtccta ggtataatgc tagcATATTA ATAGCGCCGC AATTCATGCT GCTTGCAGCC
     5701 TCTGAATTTT GTTAAATGAG GGTTAGTTTG ACTGTATAAA TACAGTCTTG CTTTCTGACC
     5761 CTGGTAGCTG CTCACCCTGA TGCTGCTGTC AATAGACAGG ATAGGTGCGC TCCCAGCAAT
     5821 AAGGGCGCGG ATGTACTGCT GTAGTGGCTA CTGAATCACC CCCGATCAAG GGGGAACCCT
     5881 AAATGGGTTG AAAGgaagag cgagatctcg agctcttcgg ctcaccttcg ggtgggcctt
     5941 tctgcgtggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     6001 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     6061 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     6121 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     6181 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6241 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6301 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6361 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6421 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6481 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6541 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6601 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6661 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6721 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6781 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6841 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6901 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6961 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7021 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7081 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7141 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7201 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7261 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7321 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7381 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7441 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7501 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7561 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7621 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7681 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7741 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7801 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7861 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7921 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcc
//