LOCUS       pUC19-t1                8696 bp ds-DNA     circular     29-MAY-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             3892..4446
                     /label="AcTniQ"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    7055..7091
                     /label="DR"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    7020..7054
                     /label="J23119"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             4605..6533
                     /label="AcCas12k"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(2860..2887)
                     /label="R"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        1166..1196
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        4556..4586
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             3084..3935
                     /label="AcTnsC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             7988..8647
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     RBS             1197..1208
                     /label="RBS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    7092..7122
                     /label="FnPSP1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    7130..7166
                     /label="DR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     RBS             4587..4598
                     /label="RBS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    7685..7789
                     /label="ampR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             1215..3080
                     /label="AcTnsB"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      49..731
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
       61 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      121 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      181 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      241 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      301 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      361 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      421 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      481 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      541 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      601 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      661 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      721 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      781 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      841 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      901 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      961 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     1021 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     1081 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctccaaaacg
     1141 atctcaagaa gatcatctta ttaattttac actttatgct tccggctcgt atgttgaaag
     1201 aggagaaagg atctatggca gacgaagaat ttgaatttac tgaaggaacg acgcaagttc
     1261 cagatgctat tttgcttgac aagagtaatt ttgtggtaga tccatcccaa attattctgg
     1321 caacgtcgga tagacataaa ctgacattta atctaatcca gtggcttgct gaatctccca
     1381 accgcactat taagtctcag agaaaacagg cagttgcaaa tacccttgat gtttctactc
     1441 gccaggtgga acgtcttctc aagcaatacg atgaagacaa gttaagagag acagcaggaa
     1501 tagaacgagc cgataaggga aaatatcgag ttagcgaata ttggcaaaac ttcatcacaa
     1561 caatctatga aaagagtctg aaagaaaaac atccaatatc accagcatcc atagttcgtg
     1621 aagtgaagcg acacgcaatt gtggatcttg aacttaagct aggagaatat cctcatcaag
     1681 ccactgttta tagaatttta gatcctttaa tcgagcaaca gaaacggaaa acaagagtta
     1741 gaaatccggg ttcgggatct tggatgacag tagtaacacg agatggagag ttacttaggg
     1801 ctgactttag taaccaaatt attcagtgtg accatactaa attggatgtt cgcatagttg
     1861 ataatcatgg caatttactg tctgatcgtc cttggctaac tactattgtg gatacttttt
     1921 caagctgtgt tgttggtttt cgcttatgga ttaaacaacc cggttctaca gaggtggctt
     1981 tagctttaag acacgctatt ttacctaaaa actaccctga agattatcaa cttaataagt
     2041 cttgggatgt atgtggacac ccctatcaat atttttttac tgatggtggt aaagattttc
     2101 gctcaaaaca tctcaaagct attggtaaga aattaggatt tcagtgtgaa ttacgcgatc
     2161 gcccaccgga aggtggtatt gtggaacgga ttttcaaaac tattaatact caagttctca
     2221 aagagttacc tggttataca ggggcaaatg ttcaggaacg cccagaaaat gcagagaaag
     2281 aagcctgttt aactattcag gatttggata agattctcgc tagtttcttt tgtgatatct
     2341 ataatcacga gccttatcct aaagagcctc gtgatacgag atttgaacgc tggtttaagg
     2401 gtatgggagg aaaactacct gaacctttgg atgagcgaga attagatatt tgtttgatga
     2461 aagaagccca acgagttgtt caagctcatg gatctattca atttgaaaac ctgatttatc
     2521 ggggagaatt tctcaaagca cataaaggtg aatatgtaac gctgagatat gatccagatc
     2581 atatcctgag tttatatatc tacagtggtg aaactgatga taatgcagga gaatttttgg
     2641 gttatgctca tgccgttaat atggataccc atgatttaag tatagaagaa ttaaaagccc
     2701 tgaataaaga gagaagtaat gctcgtaagg agcattttaa ctatgatgct ttattagcat
     2761 tgggtaaacg taaagaactt gtagaggaac ggaaagagga taaaaaggca aaaagaaact
     2821 cagaacaaaa gcgtctccgt tctgcatcca agaaaaattc caatgttatt gaactacgca
     2881 aaagtaggac ttccaaatct ttgaagaaac aagaaaatca ggaagtttta ccagagagaa
     2941 tttccaggga agaaatcaag cttgagaaga tagaacagca accacaggaa aatctatcag
     3001 cttcacctaa cactcaagaa gaagagagac ataagttagt tttctctaac cgtcaaaaaa
     3061 atttgaacaa gatttggtaa attatggcgc aacctcaact tgcaactcaa tctattgttg
     3121 aagtcctagc cccaaggtta gacatcaaag ctcaaattgc taaaactatt gatattgaag
     3181 agatttttag agcttgtttt atcactactg atcgggcttc ggaatgcttc agatggttag
     3241 atgaattgcg tattctcaaa caatgtggtc gaatcattgg accaagaaat gtgggaaaaa
     3301 gcagagccgc gcttcactat cgagatgagg ataaaaaacg agtttcctat gtaaaggctt
     3361 ggtctgcatc gagttctaag cggctatttt cacaaatcct gaaggatatt aatcatgctg
     3421 caccaacagg taaacgacag gatttacgtc caagattagc gggtagtctg gaactatttg
     3481 gattggaatt ggtgattata gataatgcgg aaaatcttca aaaagaagca ctgctagact
     3541 tgaaacaact ttttgaagag tgtaatgttc ctattgtttt agctggaggt aaggagttag
     3601 atgatctttt acacgattgt gatttgttga ctaatttccc aacactctat gagtttgaac
     3661 ggttggaata tgatgatttc aaaaaaacat taactacaat tgaattggat gttttatctc
     3721 ttccagaagc atctaattta gctgagggca atatttttga gattttagca gttagtacag
     3781 aagcacgaat gggaatttta atcaagatac taactaaggc tgttttacat tctctcaaaa
     3841 atggatttca ccgagttgat gaaagtattt tagaaaaaat tgctagtcgt tatggcacaa
     3901 aatatattcc tctcaaaaac agaaataggg attgatgaag atgatgaaat tcgcccaaag
     3961 ttaggctatg ttgaacctta tgaggaggag agtattagtc attatctagg gcgtttgcga
     4021 cggtttaagg ctaacagcct accgtcagga tactctttgg gaaaaattgc tggactcggt
     4081 gcaatgattt cacgttggga gaagctttat ttcaatcctt ttcctactct acaagagttg
     4141 gaggctttgt cctctgtggt gggagttaat gcagatagat taatagaaat gctcccctct
     4201 cagggaatga cgatgaagcc tagaccaatt aggttatgtg gggcttgtta tgcagaatct
     4261 ccttgtcatc ggattgagtg gcagtgtaag gatagaatga aatgcgatcg ccacaattta
     4321 cgtttattaa taaaatgtac taattgtgaa actcctttcc cgattcccgc agattgggtt
     4381 aaaggtcaat gtcctcattg ttccctgcct tttgcaaaga tggcgaaaag gcaaaggcgt
     4441 gattagattg tgggaataag gccattatta cagagtgctt tgcaattctt acggagagat
     4501 ttgcgatcgc acctcagtta caaacaagaa agagatgtga acaccacgga aattatttac
     4561 actttatgct tccggctcgt atgttgaaag aggagaaagg atctatgagc gttatcacaa
     4621 ttcaatgtcg cttggttgct gaagaagaca gcctccgtca actatgggaa ttgatgagtg
     4681 aaaaaaatac accattcatc aatgaaattt tgctacagat aggaaaacac ccagaatttg
     4741 aaacctggct agaaaaaggt agaataccgg ctgaattact caaaacactg ggtaactccc
     4801 tgaaaactca agaacctttt actggacaac ctggacgttt ttacacctca gcgattactt
     4861 tagtggatta tctgtataaa tcctggtttg ctttacagaa acgcagaaag cagcaaatag
     4921 aagggaaaca gcgttggcta aaaatgctca aaagtgatca agaacttgag caagaaagtc
     4981 aatctagctt agaagtaatc cgtaataaag ccactgaact ttttagcaaa tttacccctc
     5041 agtccgatag cgaagcgctc cgtaggaatc aaaatgacaa acagaaaaag gtaaaaaaga
     5101 ctaaaaaatc cacaaaaccg aaaacatctt caattttcaa aattttttta agcacttacg
     5161 aagaagcgga agaacctctt actcgttgcg ctcttgcata tctactcaaa aataactgtc
     5221 aaattagtga actggatgaa aacccagaag aatttaccag aaataagcgc agaaaagaaa
     5281 tagaaattga gcgattaaaa gatcaactcc aaagtcgcat ccccaaaggt agagatttga
     5341 caggagaaga atggttagaa accttagaaa ttgccacctt caatgttccg caaaatgaaa
     5401 atgaagcaaa agcatggcaa gcagcacttt taagaaaaac tgctaatgtt ccctttcctg
     5461 tagcttatga atctaacgag gatatgacat ggttaaagaa tgataaaaat cgtctctttg
     5521 tacggttcaa tggcttggga aaacttactt ttgagattta ctgcgataag cgtcatttgc
     5581 actacttcca acgcttttta gaggatcaag aaattctacg caatagtaaa aggcagcact
     5641 caagcagttt gtttactcta cgctcaggaa gaatagcttg gttgccaggt gaagaaaaag
     5701 gtgaacattg gaaagtaaat caactaaatt tttattgttc tttagatact cgaatgctga
     5761 ctaccgaagg aactcaacag gtagttgagg agaaagttac agcaattacc gaaattttaa
     5821 ataaaacaaa acagaaagat gatctcaacg ataaacaaca agcttttatt actcgtcagc
     5881 aatcaacact agctcgaatt aataaccctt ttcctcgtcc cagtaaacct aattatcaag
     5941 gtaaatcttc tatcctcata ggtgttagtt ttggactaga aaaaccagtc acagtagcag
     6001 tcgtagatgt tgttaaaaat aaagttatag cttatcgcag tgtcaaacaa ctacttggtg
     6061 aaaactataa tcttctgaat cgtcagcgac aacaacagca acgcctatct cacgaacgcc
     6121 acaaagccca aaaacaaaat gcacccaact cttttggtga atctgaatta ggacaatatg
     6181 tggatagatt gttagcagat gcaattattg cgatcgctaa aaaatatcaa gctggcagta
     6241 tagttttacc caaactccgc gatatgcgag agcaaatcag cagtgaaatt caatccagag
     6301 cagaaaatca atgccctggt tacaaagaag gccaacaaaa atacgccaaa gaatatcgaa
     6361 taaacgttca tcgctggagt tatggacgat taatcgagag tatcaaatcc caagcagcac
     6421 aagctggaat tgcaattgaa actggaaaac agtcaatcag aggcagtcca caagaaaaag
     6481 cacgagattt agccgtcttt acttaccaag aacgtcaagc tgcgctaatt tagtttacta
     6541 atccgaacct tgacaatata atatggatac aacagcgccg tagttcatgc tccttggagt
     6601 ctctgtacta tgaaaaatct ggcttagttt ggcagttgga agactgtcat gctttctgag
     6661 cctggtagct gcccgcttct gatgctgctg tcgcaagaca ggataggtgc gctcccagca
     6721 ataaggagta aggcttttag ccatagtcgt tatttataac gatgtggatt tccacagtgg
     6781 tggctactga atcaccccct tcgtcggggg aatcctccca aatctttttt ttggcgaacc
     6841 ataagcgggg tcaaaaaccc tggggatctg ccaaaacctt gaatcccttg tccagtattt
     6901 atttgactca tttgaagagt gatgaatgtc ctctattgag agcaaaaaaa cagatttttt
     6961 aacaggtttg ccaaaatcgc atctggaaac ctgtattggc aagggtctag acgggcgcgt
     7021 tgacagctag ctcagtccta ggtataatgc tagcgtttca actaccatcc cgactagggg
     7081 tgggttgaaa gGAGAAGTCA TTTAATAAGG CCACTGTTAA AActccaaag tttcaactac
     7141 catcccgact aggggtgggt tgaaagcggc cgacgcgctg ggctacgtca attcactggc
     7201 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     7261 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     7321 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     7381 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     7441 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     7501 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     7561 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     7621 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     7681 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7741 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7801 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7861 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7921 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7981 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8041 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8101 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8161 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8221 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8281 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8341 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8401 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8461 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8521 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8581 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8641 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaac
//