LOCUS Exported 6746 bp ds-DNA circular SYN 04-APR-2018 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6746) AUTHORS HMS Neurobiology TITLE Direct Submission JOURNAL Exported Apr 4, 2018 from SnapGene 4.1.7 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6746 /organism="recombinant plasmid" /mol_type="other DNA" misc_feature complement(167..786) /label=pBR322_origin misc_feature complement(941..1801) /label=Ampicilin misc_feature complement(1843..1871) /label=Ampr promoter misc_feature 2458..2645 /label=ITR misc_feature 2649..3585 /label=CAG (CBA) CDS 3595..3696 /codon_start=1 /label=iGlucose N-terminal leader /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRW" misc_feature 3607..3627 /label=His6Gly /note="His6Gly" misc_feature 3628..3657 /label=T7 gene10 leader /note="T7 gene10 leader" misc_feature 3661..3687 /label=Xpress epitope /note="Xpress epitope" CDS 3697..4677 /codon_start=1 /label=TtGBP 1- 326 /note="TtGBP 1- 326" /translation="GSKLEIFSWWAGDEGPALEALIRLYKQKYPGVEVINATVTGGAGV NARAVLKTRMLGGDPPDTFQVAAGMELIGTWVVANRMEDLSALFRQEGWLQAFPKGLID LISYKGGIWSVPVNIHRSNVMWYLPAKLKEWGVNPPRTWDEFLATCQTLKQKGLEAPLA LGENWTQQHLWESVALAVLGPDDWNNLWNGKLKFTDPKAVRAWEVFGRVLDCANKDAAG LSWQQAVDRVVQGKAAFNVMGDWAAGYMTTTLKLKPGTDFAWAPSPGTQGVFMMLSDSF GLPKGAKNRQNAINWLRLVGSKEGQDTFNPLKGSIAARLDSDPSKY" misc_feature 3697..3735 /label=s.Synth.42.1 /note="s.Synth.42.1" CDS 4687..5421 /codon_start=1 /label=cpmT-Sapphire /note="cpmT-Sapphire" /translation="FTAHNVYIMADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIG DGPVLLPDNHYLSIQSKLSKDPNEKRDHMVLLEFVTAAGITHGMDELYKGGTGGSMVSK GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVT TFSYGVMVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVN RIELKGIDFKEDGNILGHKLEYN" misc_feature 5428..5634 /label=TtGBP 326-392 /note="TtGBP 326-392" misc_feature 5650..6237 /label=WPRE misc_feature complement(6276..6395) /label=SV40_PA_terminator misc_feature complement(6503..6692) /label=ITR ORIGIN 1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta 1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt 2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg 2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag 2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag 2641 gaagagtacc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 2701 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 2761 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 2821 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 2881 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc 2941 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg 3001 gggcgggggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg 3061 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt 3121 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag 3181 tcgctgcgac gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc 3241 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg 3301 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc 3361 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcggggct gtccgcgggg 3421 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg 3481 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg 3541 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattggtac caccatgcgg 3601 ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt 3661 cgggatctgt acgacgatga cgataaggac cgatggggat ccaaactgga aatttttagc 3721 tggtgggccg gcgatgaagg cccggccctg gaagccctga ttcgcctgta taaacagaaa 3781 tatccgggcg tggaggtgat taacgccacc gtgaccggcg gcgccggcgt gaacgcccgc 3841 gccgtgctga aaacccgcat gctgggcggc gatccgccgg atacctttca ggtggcagcc 3901 ggcatggaac tgattggcac ctgggtggtg gccaaccgca tggaagacct gagcgccctg 3961 tttcgccagg aaggctggct gcaagccttt ccgaaaggcc tgattgatct gattagctat 4021 aaaggcggca tttggagcgt gccggtgaac attcatcgca gcaacgtgat gtggtatctg 4081 ccggccaaac tgaaagaatg gggcgtgaac ccgccgcgca cctgggatga atttctggcc 4141 acctgccaga ccctgaaaca gaaaggcctg gaagccccgc tggccctggg cgaaaactgg 4201 acccagcagc atctgtggga aagcgtggcc ctggctgtgc tgggcccgga tgattggaac 4261 aacctgtgga acggcaaact gaaatttacc gatccgaaag ccgtgcgcgc ctgggaagtg 4321 tttggccgcg tgctggattg cgccaacaaa gatgccgccg gcctgagctg gcagcaggcc 4381 gtggatcgcg tggtgcaggg caaagccgcc tttaacgtga tgggcgattg ggccgccggc 4441 tatatgacca ccaccctgaa actgaaaccg ggcaccgatt ttgcctgggc cccgagcccg 4501 ggcacccagg gcgtgtttat gatgctgagc gatagctttg gcctgccgaa aggcgccaaa 4561 aaccgccaga acgccattaa ctggctgcgc ctggtgggca gcaaagaagg ccaggatacc 4621 tttaacccgc tgaaaggcag cattgccgcc cgcctggatt ctgatccgag taaatatccc 4681 gattctttca ctgctcacaa cgtgtatatc atggctgaca aacagaagaa cggtatcaag 4741 gccaacttca agatccgcca caacatcgaa gacggcggcg ttcagctggc tgatcactac 4801 cagcagaaca ccccaatcgg cgacggccct gttctgctgc ctgacaacca ctacttaagc 4861 atccagtcta aactgtccaa agacccaaac gagaagcgcg accacatggt tctgctggaa 4921 tttgttaccg ccgctggcat cacccacggc atggacgaac tgtacaaggg cggcaccggg 4981 ggatctatgg tttccaaagg cgaggaactg ttcactggtg ttgttcctat cctggttgaa 5041 ctggacggcg acgttaacgg ccacaaattc agcgttagcg gggaaggcga aggagacgct 5101 acctacggca agctgactct gaagttcatc tgcaccactg gcaagctgcc tgttccttgg 5161 cctaccctgg ttactacctt ctcctatggt gttatggttt tcgccagata tcctgaccac 5221 atgaagcagc acgacttctt caagagcgcc atgcctgagg gctacgttca ggaacgcacc 5281 atctttttca aggacgacgg caactataag acacgcgctg aggttaagtt cgagggcgac 5341 actctggtta accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 5401 ggccataagc ttgaatataa cgagcccaac gcctatggtc agagtgcaat gagagattgg 5461 cgcagcaaca gaattgtggg cagcctggtg gcaggcgccg tggccccgga aagctttatg 5521 agccagtttg gcaccgtgat ggaaattttt ctgcaaaccc gcaacccgca ggccgccgcc 5581 aacgccgccc aggccattgc cgatcaggtg ggcctgggcc gcctgggcca gtaagactag 5641 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 5701 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 5761 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 5821 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 5881 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 5941 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 6001 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg 6061 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 6121 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 6181 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg 6241 ataccgtcga cccgggcggc cgcttcgagc agacatgata agatacattg atgagtttgg 6301 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat 6361 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca 6421 ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta 6481 caaatgtggt aaaatcgata aggatcttcc tagagcatgg ctacgtagat aagtagcatg 6541 gcgggttaat cattaactac aaggaacccc tagtgatgga gttggccact ccctctctgc 6601 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc 6661 gggcggcctc agtgagcgag cgagcgcgca gctgcattaa tgaatcggcc aacgcgcggg 6721 gagaggcggt ttgcgtattg ggcgct //