LOCUS       Exported               15392 bp ds-DNA    circular SYN 13-OCT-2016
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pC13N-dCas9-BFP-KRAB (151217 seq ver #G8)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 28, 2019 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15392
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    9..42
                     /bound_moiety="Cre recombinase"
                     /note="lox2272"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     promoter        52..1666
                     /note="CAG Promoter"
     intron          1673..1766
                     /note="Mini-Intron"
     misc_feature    1759..1773
                     /note="Feature 30"
     misc_feature    1769..1774
                     /note="Bsrg1 site used for cloning"
     misc_feature    1775..1780
                     /note="part of primer"
     misc_feature    1781..6955
                     /note="PCR product"
     misc_feature    1781..1801
                     /note="Feature 27"
     CDS             1787..6955
                     /codon_start=1
                     /label="dCas9-HA-NLS-BFP-KRAB"
                     /note="dCas9-HA-NLS-BFP-KRAB"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGDAYPYDVPDYASLGSGSPKKKRKVEDPKKKRKVDG
                     IGSGSNGSSGSSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGG
                     PLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDT
                     SLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGG
                     SHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPS
                     KLGHKLNGGGGGMDAKSLTAWSRTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENY
                     KNLVSLGYQLTKPDVILRLEKGEEP"
     misc_feature    1787..5890
                     /note="dCas9"
     misc_feature    5894..5920
                     /label="HA"
                     /note="HA"
     misc_feature    5939..5959
                     /label="SV40 NLS"
                     /note="SV40 NLS"
     misc_feature    5966..5986
                     /note="SV40 NLS"
     misc_feature    6935..6955
                     /note="Feature 31"
     misc_feature    6957..6971
                     /note="Feature 32"
     misc_feature    6965..7491
                     /label="Rabbit beta-globin polyA signal"
                     /note="Rabbit beta-globin polyA signal"
     protein_bind    complement(7498..7531)
                     /bound_moiety="Cre recombinase"
                     /note="loxP511"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
     misc_feature    7564..8774
                     /label="chick cHS4 insulator"
                     /note="chick cHS4 insulator"
     misc_feature    8783..9578
                     /label="C13T1-RA"
                     /note="C13T1-RA"
     CDS             9675..9743
                     /label="LacZ alpha"
                     /note="LacZ alpha"
     CDS             10095..10904
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      11082..11670
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11958..11979
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11994..12024
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    12032..12048
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    12093..12924
                     /label="C13-LA"
                     /note="C13-LA"
     protein_bind    12933..12966
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    12967..12992
                     /note="Splice acceptor"
     CDS             13016..13069
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             13073..13864
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    13899..14123
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    14124..14157
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    14158..15368
                     /label="chick cHS4 insulator(1)"
                     /note="chick cHS4 insulator(1)"
ORIGIN
        1 cggtcgacat aacttcgtat aggatacttt atacgaagtt atcaattcag gctagttatt
       61 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      121 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      181 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      241 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      301 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      361 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
      421 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
      481 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc
      541 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
      601 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
      661 ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac gctgccttcg
      721 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
      781 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
      841 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
      901 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      961 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     1021 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     1081 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     1141 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
     1201 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
     1261 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     1321 ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg
     1381 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
     1441 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa
     1501 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
     1561 ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg
     1621 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
     1681 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
     1741 gctgtctcat cattttggca aagaattgtg tacaactagt gccaccatgg acaagaagta
     1801 cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta
     1861 caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa
     1921 gaacctgatc ggcgccctgc tgttcgacag cggagaaaca gccgaggcca cccggctgaa
     1981 gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat
     2041 cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt
     2101 cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga
     2161 ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag
     2221 caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg
     2281 gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt
     2341 catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg
     2401 cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct
     2461 gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggcaacctga ttgccctgag
     2521 cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca
     2581 gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca
     2641 gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat
     2701 cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata
     2761 cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga
     2821 gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct acatcgatgg
     2881 cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg
     2941 caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt
     3001 cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg
     3061 gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac
     3121 cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat
     3181 gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg
     3241 cgccagcgcc cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga
     3301 gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtaca acgagctgac
     3361 caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa
     3421 aaaagccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa
     3481 agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga
     3541 tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga
     3601 cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact
     3661 gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga
     3721 caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa
     3781 gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc
     3841 cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa
     3901 agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc
     3961 caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga
     4021 cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag
     4081 agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga
     4141 agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca
     4201 gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga
     4261 ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gacgctatcg tgcctcagag
     4321 ctttctgaag gacgactcca tcgataacaa agtgctgact cggagcgaca agaaccgggg
     4381 caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcgcca
     4441 gctgctgaat gccaagctga ttacccagag gaagttcgac aatctgacca aggccgagag
     4501 aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg
     4561 gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga
     4621 gaacgacaaa ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga
     4681 tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca
     4741 cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga
     4801 aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag
     4861 cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt
     4921 tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac
     4981 aaacggcgaa acaggcgaga tcgtgtggga taagggccgg gactttgcca ccgtgcggaa
     5041 agtgctgtct atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt
     5101 cagcaaagag tctatcctgc ccaagaggaa cagcgacaag ctgatcgcca gaaagaagga
     5161 ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt
     5221 ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg
     5281 gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa
     5341 gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga
     5401 gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga
     5461 actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct
     5521 gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc acaaacacta
     5581 cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc
     5641 taatctggac aaggtgctga gcgcctacaa caagcacaga gacaagccta tcagagagca
     5701 ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa
     5761 gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc
     5821 caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct
     5881 gggaggcgac gcctatccct atgacgtgcc cgattatgcc agcctgggca gcggctcccc
     5941 caagaaaaaa cgcaaggtgg aagatcctaa gaaaaagcgg aaagtggacg gcattggtag
     6001 tgggagcaac ggcagcagcg gatccagcga gctgattaag gagaacatgc acatgaagct
     6061 gtacatggag ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa
     6121 gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt
     6181 cgccttcgac atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac
     6241 ccagggcatc cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt
     6301 caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg
     6361 ctgcctcatc tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat
     6421 gcagaagaaa acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg
     6481 cctggaaggc agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa
     6541 catcaagacc acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta
     6601 ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca
     6661 gcacgaggtg gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa
     6721 tggcggtggc ggagggatgg atgctaagtc actaactgcc tggtcccgga cactggtgac
     6781 cttcaaggat gtatttgtgg acttcaccag ggaggagtgg aagctgctgg acactgctca
     6841 gcagatcgtg tacagaaatg tgatgctgga gaactataag aacctggttt ccttgggtta
     6901 tcagcttact aagccagatg tgatcctccg gttggagaag ggagaagagc cctagacgcg
     6961 tgaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     7021 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     7081 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     7141 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     7201 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     7261 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     7321 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     7381 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     7441 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat caccggtata
     7501 acttcgtata atgtatacta tacgaagtta tggtacctta attaagttaa catgcatagt
     7561 actgagctca cggggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc
     7621 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca
     7681 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
     7741 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa
     7801 aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc ctgggttgca
     7861 aaggagcaca gtgctcatcc agatccaacc ccctgctatg tgcagggtca tcaaccagca
     7921 gcccaggctg cccagagcca catccagcct ggccttgaat gcctgcaggg atggggcatc
     7981 cacagcctcc ttgggcaacc tgttcagtgc gtcaccaccc tctgggggaa aaactgcctc
     8041 ctcatatcca acccaaacct cccctgtctc agtgtaaagc cattccccct tgtcctatca
     8101 agggggagtt tgctgtgaca ttgttggtct ggggtgacac atgtttgcca attcagtgca
     8161 tcacggagag gcagatcttg gggataagga agtgcaggac agcatggacg tgggacatgc
     8221 aggtgttgag ggctctggga cactctccaa gtcacagcgt tcagaacagc cttaaggata
     8281 agaagatagg atagaaggac aaagagcaag ttaaaaccca gcatggagag gagcacaaaa
     8341 aggccacaga cactgctggt ccctgtgtct gagcctgcat gtttgatggt gtctggatgc
     8401 aagcagaagg ggtggaagag cttgcctgga gagatacagc tgggtcagta ggactgggac
     8461 aggcagctgg agaattgcca tgtagatgtt catacaatcg tcaaatcatg aaggctggaa
     8521 aagccctcca agatccccaa gaccaacccc aacccaccca ccgtgcccac tggccatgtc
     8581 cctcagtgcc acatccccac agttcttcat cacctccagg gacggtgacc cccccacctc
     8641 cgtgggcagc tgtgccactg cagcaccgct ctttggagaa ggtaaatctt gctaaatcca
     8701 gcccgaccct cccctggcac aacgtaaggc cattatctct catccaactc caggacggag
     8761 tcagtgagaa tattgtttaa actcctcatc cttccaacat aaatatattt tgggattata
     8821 tcaacattca atgttactta aagtgacctt gtaaatattt tcacaactga gccatgtttg
     8881 atttgtatac ttatgtttac tttactgttt ttcctgaagt taataattgc cttgaattta
     8941 tttatttctt taaaaatgtt tcattactca ggactgtagt ttacattacg attctttgtg
     9001 ttatacagtt gatgggtttc ttttctttct taatttcttt aaaaaataga gatggggtct
     9061 tactatatta cccaggctgg tcttgaagtc ctgggctcaa gtgatcttcc tgtctcagcc
     9121 taccaagtag ctgagactat aggtgcaaaa aagccactat acctggctag tttacaggtt
     9181 ttaacaaatg cattatgcca cgtatccatt attacaggat cacacaagat attttcatta
     9241 ccctgaaagc atccctgtgt tccaccaatt catcctgcct ccatgagccg ctggcaacca
     9301 ctgatctcta tagttttgcc ttttctaaaa tgtcatataa ttggaatcat acagtctgta
     9361 gcattttcag actagctttt aaaatttggc aatatgcatt taaggttcct ccttaaatgt
     9421 gaagggcata gccaatgtgg cttgatagct catttctttt tattggtgaa tatttcattg
     9481 tctggatgtt ccacagtttg tttatccatt cacctattca atttgctttt tttctgtgta
     9541 tctatcacta attcaatact ggactctcca acagagccgt ttaaacactg gccgtcgttt
     9601 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     9661 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     9721 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     9781 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     9841 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     9901 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     9961 cgtcatcacc gaaacgcgcg atgcagctct ggcccgtgtc tcaaaatctc tgatgttaca
    10021 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
    10081 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
    10141 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
    10201 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
    10261 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
    10321 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
    10381 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
    10441 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
    10501 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
    10561 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
    10621 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
    10681 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
    10741 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
    10801 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
    10861 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
    10921 ggttgtaaca ttattcagat tgggcttgat ttaaaacttc atttttaatt taaaaggatc
    10981 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    11041 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    11101 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    11161 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    11221 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    11281 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    11341 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    11401 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    11461 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    11521 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    11581 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    11641 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    11701 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    11761 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    11821 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
    11881 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
    11941 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
    12001 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
    12061 aacagctatg accatgatta cgccgcggcc gcagcaggag aatcatgcca atgggccaat
    12121 atacattctg acccacagtt tcataataaa ataaaatggt tgtggttgta agccactatg
    12181 tttcagagtg gtttgttaca cagcaataaa taactaatat agtaggcata ccatcaagtc
    12241 caaagtaggt agagaagaat gtaaatagca gagcaaaaca gcatgactgg tggctgggag
    12301 gcttaaaact gggacaggat cagagtcatg aaagaagtca aagaaatggt tcagaagtaa
    12361 ggctgagact gacttacaaa agctgaaagt ccctttaagt tggtgtttgg tgcattggca
    12421 ggggcaggta tggtgactta aaagagccat gctcaacaag atcaagcaca acacaatcac
    12481 gggtcacccc agcagacctt agcgagtcta gccatttctt tggtggtggt cacagtcatg
    12541 cttcagccca gtttccactt ggacaaatgg tacatatttt caatgagatg aaaattaaga
    12601 tacaatccat gtgctcagag agtgatcaca gctctgacta aacactgtgc cccaaagtgt
    12661 tgaggaattg ggaaaaccta gctgagttag tggtctcttt tctgttacaa taaagctcat
    12721 aatgaaaatt agccttcttt gttcttcccc aagtctttct ttctagacga aactactttc
    12781 aactgtttta acttccttac tgttaacttc catatttcta gatagtatgg tcagactgtt
    12841 atttcttgac ttttaaatgt aagatattat ctactgactt ccttctatgt aagatgagga
    12901 ttgagctctc ttacccttct cccagcggcc gcataacttc gtatagcata cattatacga
    12961 agttatctga cctcttctct tcctcccaca gggcctcgag agatctggca gcggagaggg
    13021 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta ggattgaaca
    13081 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
    13141 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
    13201 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
    13261 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
    13321 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
    13381 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
    13441 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
    13501 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
    13561 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
    13621 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
    13681 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
    13741 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
    13801 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
    13861 ctaacacctg gtgcatgacc cgcaagcccg gtgcctgact gtgccttcta gttgccagcc
    13921 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
    13981 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
    14041 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
    14101 tggggatgcg gtgggctcta tggataactt cgtatagcat acattatacg aagttatgag
    14161 ctcacgggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
    14221 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
    14281 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
    14341 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct
    14401 ttaggctgaa agagagattt agaatgacag aatcatagaa cggcctgggt tgcaaaggag
    14461 cacagtgctc atccagatcc aaccccctgc tatgtgcagg gtcatcaacc agcagcccag
    14521 gctgcccaga gccacatcca gcctggcctt gaatgcctgc agggatgggg catccacagc
    14581 ctccttgggc aacctgttca gtgcgtcacc accctctggg ggaaaaactg cctcctcata
    14641 tccaacccaa acctcccctg tctcagtgta aagccattcc cccttgtcct atcaaggggg
    14701 agtttgctgt gacattgttg gtctggggtg acacatgttt gccaattcag tgcatcacgg
    14761 agaggcagat cttggggata aggaagtgca ggacagcatg gacgtgggac atgcaggtgt
    14821 tgagggctct gggacactct ccaagtcaca gcgttcagaa cagccttaag gataagaaga
    14881 taggatagaa ggacaaagag caagttaaaa cccagcatgg agaggagcac aaaaaggcca
    14941 cagacactgc tggtccctgt gtctgagcct gcatgtttga tggtgtctgg atgcaagcag
    15001 aaggggtgga agagcttgcc tggagagata cagctgggtc agtaggactg ggacaggcag
    15061 ctggagaatt gccatgtaga tgttcataca atcgtcaaat catgaaggct ggaaaagccc
    15121 tccaagatcc ccaagaccaa ccccaaccca cccaccgtgc ccactggcca tgtccctcag
    15181 tgccacatcc ccacagttct tcatcacctc cagggacggt gaccccccca cctccgtggg
    15241 cagctgtgcc actgcagcac cgctctttgg agaaggtaaa tcttgctaaa tccagcccga
    15301 ccctcccctg gcacaacgta aggccattat ctctcatcca actccaggac ggagtcagtg
    15361 agaatattgg cgcgccgaat tctcgcgacg at
//
